New Species and First Record of Dannella Edmunds 1959 (Ephemeroptera: Ephemerellidae) from the Eastern Palaearctic.

A new species, Dannella daurica sp. nov. is described from the Far East of Russia, based on larvae. Characters of this new species agree with the diagnosis of Dannella Edmunds 1959 in the restricted sense. Comparison of the larva of Dannella daurica sp. nov. with other known larvae of Dannella is given. Until now, the genus Dannella was known only from the Nearctic Region. This is the first report of this genus from the East Palaearctic Region.

Environmental protection measures in mineral resource development: case study of a gold-bearing deposit in the Russian Far East.

In the Russian mining industry, the recent social and economic processes inevitably affect environmental safety and the social security of all those affected by mining. Thus, this study aimed to evaluate the technogenic impacts of a mining company on the environment. Measures were developed and implemented to ensure ecological safety and social security during a mineral resource development project in the southern part of the Russian Far East. This study analysed global experiences in this regard and carried out field research with the aim of establishing an inventory of plants and animals (terrestrial and aquatic), showing that technogenesis produced new specific landforms, e.g. quarries and dumps that replaced natural landforms. The main ecologically negative impacts of the mining operations in the region were the movement of mountain masses, changes in forms of erosion, and destruction of mountain ranges with the formation of dispersed clastic fractions of large specific surface areas, which determine exomorphodynamic processes, e.g. deflation, suffusion, and landslides. A general assessment of the biota status and natural water quality within the boundaries of influence of the developed deposit was presented, and a set of measures was recommended for environmental protection and ensuring the rational use of natural resources during mining operations. Moreover, the necessity of creating effective mining and environmental monitoring systems was supported. A 'Map of the Ecological State of Gold Mining Development in the Albazino Territory' was compiled for the first time, pinpointing areas undergoing various degrees of environmental stress. Changes in the forested areas within the territory of the mining allotment were forecast using the forest cover of the study area as the baseline.

Baetis majus sp. nov., new species of mayfly (Ephemeroptera: Baetidae) from Far East of Russia.

A new species, Baetis majus Tiunova sp. nov., is described and illustrated based on larvae and reared adults discovered in the Russian Far East. The differential identification of this species was determined by the characteristics of other representatives of the genus Baetis Leach, including subgenera Baetis Leach and Tenuibaetis Kang Yang from Eastern and Western Palaearctic, Nearctic and Oriental regions. In addition to morphological studies, DNA barcoding of the described species with average intraspecific K2P distances to nearest neighbours is documented. We reconstructed the phylogenetic relationships of all available cytochrome c oxidase subunit I (COI) sequences of the subgenera of Baetis and Tenuibaetis from four regions. Bayesian analysis using 47 morphological characters additional to partial COI sequences did not allow to determine the species-group of the Baetis genus to which the described species belongs.

Description of the larva of Cinygmula levanidovi Tshernova amp; Belov, 1982(Ephemeroptera, Heptageniidae) with redescription of the male adult from the Russian Far East.

The larvae, male and female imagines, and eggs of Cinygmula levanidovi Tshernova Belov 1982 are described based on reared specimens from the Russian Far East. The larvae, female imago and eggs are described and illustrated for the first time. The larva of C. levanidovi is similar to the one of C. hirasana Imanishi, 1935 and C. kurenzovi (Bajkova, 1965). However, it can be distinguished from these species and from all other Far Eastern Cinygmula by the shape of its tergalius I, which has a heart-shape and bears a single short gill filament. Tergalius I of C. hirasana and C. kurenzovi possess a similar shape, but there are no gill filaments on the first and the other tergalii.

Baetis (Rhodobaetis) molecularis sp. nov., a new mayfly species (Ephemeroptera: Baetidae) from the Russian Far East.

A new species Baetis (Rhodobaetis) molecularis sp. nov. is described and illustrated based on larvae and reared adults from the Far East of Russia. The differential diagnosis of this species is provided with regard to other representatives of the subgenus Rhodobaetis Jacob, 2003 from East Palaearctic and Nearctic Regions. A dataset including novel and publicly available COI mtDNA sequences of 16 species of Rhodobaetis has been assembled to provide a reference dataset for DNA barcoding. The comparison between Baetis (Rhodobaetis) molecularis sp. nov. and other species produced K2P genetic distances of 0.201 in average, values well above those associated with intraspecific variation. The closest species was Baetis foemina McDonough with a K2P distance value 0.114. A Bayesian phylogeny of available Rhodobaetis is also provided.

Baetis pentaphyllus sp. nov., a new species of mayfly (Ephemeroptera: Baetidae) from the Russian Far East.

A new species, Baetis pentaphyllus sp. nov., is described on the basis of larvae from the Far East of Russia (type locality Bolshoi Garmakan River). Larvae of Baetis pentaphyllus sp. nov. may be distinguished from other Baetis species by the presence of only five pairs of tergalii on segments III-VII. The mitochondrial COI sequence obtained from the described species was compared with the data present in GeneBank and BOLD. The DNA barcodes allowed discrimination of B. pentaphyllus sp. nov. from other species of Baetis with available sequence data. The average interspecific K2P distances were 10-15%, which are values well above those associated with intraspecific variation. COI sequences as well as 36 morphological larval characters were analysed using Bayesian inference to relate the described species to the recognized species-groups of the Baetis genus. B. pentaphyllus sp. nov formed a sister clade to B. vardarensis + B. lutheri which belong to the Baetis lutheri species-group.

A new species of Paraleptophlebia Lestage, 1917 (Ephemeroptera: Leptophlebiidae) and a re-description of adults for Paraleptophlebia westoni Imanishi, 1937 with the first description of the larva, from the Russian Far East.

Male, female imagoes and eggs of Paraleptophlebia kunashirica sp. nov. and the larva, male and female imagoes of Paraleptophlebia westoni are described based on material from the Russian Far East. Comparison of the male imago of Paraleptophlebia kunashirica sp. nov. and the larva and male imago of Paraleptophlebia westoni with other known larvae and imagoes of Paraleptophlebia are given. Paraleptophlebia westoni larvae may be distinguished from congeners by characters associated with the mouthparts, such as the ratio of the length of the maxillary and labial palp segments, shape of hypopharynx, and structure of the claw. Male imagoes of Paraleptophlebia kunashirica sp. nov. and Paraleptophlebia westoni сan be distinguished between themselves and other representatives close to them in the form of the ventral appendages and the apex of the penis lobes.

New species of Ameletus Eaton, 1885 from the Russian Far East with notes on Ameletus camtschaticus Ulmer 1927 (Ephemeroptera: Ameletidae).

The male imagoes, larvae, and eggs of Ameletus allengaensis sp. nov. and Ameletus sirotskii sp. nov. from the Russian Far East are described. Based on the structure of the male genitalia, the imago and larvae of A. allengaensis sp. nov. and A. sirotskii sp. nov. are similar to those of A. camtschaticus, but the discovery of these new species and separation from A. camtschaticus were confirmed by studies of the morphology of the larvae and male imago, as well as molecular analysis. Identity of various developmental stages of the new species were confirmed by analysis of the mitochondrial gene cytochrome oxidase 1 (COI) DNA barcode, which was also used to reconstruct the phylogenetic relationships within the genus Ameletus. The intraspecific sequence divergence based on the Kimura-2-parameter (K2P) distance ranged from 0.0-2.5%, whereas the interspecific sequence divergence based on the K2P distance ranged from 6.2-7.9% within A. sirotskii sp. nov., A. allengaensis sp. nov. and A. camtschaticus. Male imagoes of A. allengaensis sp. nov., A. sirotskii sp. nov., and A. camtschaticus can be distinguished by the size and location of small denticles on the ventral plate of the penis. The larvae of A. allengaensis sp. nov. differ from those of A. sirotskii sp. nov. by the size of gills I and II. In A. allengaensis sp. nov., gill I is almost twice as small as gill II; in A. sirotskii sp. nov., gill I is only slightly smaller than gill II. Both new species differ from A. camtschaticus by gill II, which does not have an anal rib on the anal margin.

Redescription of Paraleptophlebia falcula Traver 1934 with notes on status and composition of Paraleptophlebia Lestage 1917 and Neoleptophlebia Kluge 1997 (Ephemeroptera: Leptophlebiidae).

The taxon Paraleptophlebia Lestage 1917 is accepted here in generic rank, so rank of Neoleptophlebia Kluge 1997 is also raised from subgenus to genus, and the following new combinations are proposed: Neoleptophlebia adoptiva (McDunnough 1929) comb. n., N. assimilis (Banks 1914) comb. n., N. erratica (Kang & Yang 1994) comb. n., N. heteronea (McDunnough 1924) comb. n., N. japonica (Matsumura 1931) comb. n. (= Paraleptophlebia chocolata Imanishi 1937), N. mollis (Eaton 1871) comb. n., N. memorialis (Eaton 1884) comb. n., N. spina (Kang & Yang 1994) comb. n., N. swannanoa (Traver 1932) comb. n., N. temporalis (McDunnough 1926) comb. n., N. vladivostokica (Kluge 1982) comb. n. Larvae, male and female imagoes and eggs of Paraleptophlebia falcula Traver 1934 are described based on reared specimens from Siberia and Russian Far East; larvae and eggs are described for the first time. Characters of this species agree with diagnosis of Paraleptophlebia in the restricted sense; comparison of larva of P. falcula with other known larvae of Paraleptophlebia is given. Formerly this species was known as imagoes from limited area in Western Nearctic. According to the new data, it is widely distributed in northern part of Amphipacific Sector of Holarctic, and its area includes Polar Urals (Komi Republic), Siberia (Transbaikal Kray, Sakha Yakutia and Amurskaya Oblast' of Russia) and west of North America (Oregon, Idaho and Washington states of USA).

A new species, Procloeon monilistylus sp. n. (Ephemeroptera, Baetidae), from the Russian Far East.

A new, two-winged species Procloeon monilistylus sp. n. is described from the Russian Far East based on male and female imagoes reared from larvae. The larva differs from other species of Procloeon by having a deeply separated incisor and kinetodontium of the mandibles. The imago differs from all Baetidae (and mayflies in general) by the shape of the gonostyli. Formerly this species was known as "Cloeon (Centroptilum) sp. 1" sensu Kluge & Novikova 1992.

New species of Ameletus Eaton, 1885 and redescription of Ameletus longulus Sinichenkova, 1981 from the Russian Far East (Ephemeroptera: Ameletidae).

Ameletus khasanensis sp. nov. is described and Ameletus longulus Sinichenkova, 1981 is revalidated and redescribed based on material from the Russian Far East. The structure of the penis of both species is similar to that of Ameletus costalis (Matsumura 1931), a description and drawings of which are also presented in this paper. The male imago of A. khasanensis sp. nov. can be distinguished from that of A. costalis by the absence of spinules on the dorsal surface of the fore tibia. The male imago of A. longulus can be distinguished from A. khasanensis sp. nov. and A. costalis by the color of the forewings. The larvae of A. khasanensis sp. nov. differ from A. costalis in the length of the denticles on the right mandible, in the absence of the group of setae on the dorsal surface at the base of fore femora and the width/length ratio of gill VII. The larvae of A. longulus differ from A. khasanensis and A. costalis in the form and number of the denticles of the first comb-shaped seta of the maxilla and the number of setae on the dorsal surface of the base of the fore femora.

New species of Cinygmula McDunnough, 1933 and larval description of Cinygmula irina Tshernova & Belov, 1982 from the Russian Far East (Ephemeroptera: Heptageniidae).

Here, we describe Cinygmula tetramera, sp. nov. (Ephemeroptera: Heptageniidae), which has male imaginal forceps with three distal segments, based on male & female imagos reared from larvae. The structure of the penes of this species is similar to that of Cinygmula irina Tshernova & Belov, 1982, a description and drawing of which are also presented in this paper. The larvae of C. irina are described for the first time. We also describe the eggs of both species. Based on our data, egg characters provide useful data for species diagnoses in the genus Cinygmula.