[The information value of clinico-hematologic criteria for the early diagnosis of acute radiation sickness in pig-tailed macaques]. / Informativnost' kliniko-gematologicheskikh kriteriev rannei diagnostiki ostroi luchevoi bolezni u obez'ian makakov-lapunderov.

Ten pig-tailed monkeys (Macaca nemestrina) were subjected to 60Co radiation at a dose of 6.0-6.5 Gy and a dose rate of 1.2 Gy/min. Acute radiation sickness has developed in the monkeys causing their death on the 16-20 day. In spite of this, the initial reaction was weakly expressed and according to its manifestation it was impossible to evaluate severity and possible outcome of the lesion. At an early stage of the disease (6-24 hours) insufficient was uranin fluorescence in blood plasma, but more informative were the changes in adhesive properties of leukocytes the dynamics of lymphocytes (lymphopenia), reticulocytes (reticulocytopenia) and shifts in reticulograms (increased per cent of juvenile forms).

[Changes in DNA and surface properties of peripheral blood leukocytes in monkeys after total gamma irradiation]. / Izmeneniia DNK i poverkhnostnykh svoistv leikotsitov perifericheskoi krovi obez'ian pri obshchem gamma-obluchenii.

Changes of Macaca nemestrina and Rhesus blood DNA have been studied up to 5 days after the total uniform gamma-irradiation in doses 6.2 and 6.5 Gy. The content of nucleotide ATrich DNA has been evaluated in the fractions of leucocytes with the various surface adherence properties. The dynamics of the content nucleotide AT-DNA and blood leucocytes were similar at the both monkey species. The evaluation of structural state DNA in the blood nucleotide with the fluorescent dyes (ethidium bromide and 4; 6-diamidine-2-phenylindole) demonstrated that the important changes in the polynucleotide structure occurred from 6 to 24 h after irradiation and maintained up to 5 days. Adhesive capacity changes were reversible but they preceded the DNA structural changes. At 24 h postirradiation non-adhesive cells with relative higher AT-DNA content were found.

[Cytogenetic study of the bone marrow and peripheral blood lymphocytes in monkeys in long-term gamma irradiation]. / Tsitogeneticheskoe issledovanie kostnogo mozga i limfotsitov perifericheskoi krovi u obez'ian, perenesshikh prolongirovannoe gamma-obluchenie.

A comparative study of chromosome structures of bone marrow and peripheral blood cells has been carried out in 8 rhesus macaques (2-7 years of age), 4 of which survived after prolonged low-capacity (3.87 microA/kg) gamma irradiation, the total dose being 7.97 Gy (LD50/60). It has been established that prolonged low-capacity gamma irradiation was of a high mutagenic activity. Various tissues of irradiated monkeys showed differences in the frequency (4 months) and types (4-33 months) of aberrations within the period of 4 to 33 months following irradiation. Mutagenic effect characteristic of the early period after the irradiation was retained in the peripheral blood of irradiated monkeys within the period of observation.

[Dynamics of the chromosome aberrations in the bone marrow cells of monkeys after prolonged irradiation]. / Dinamika aberatsii khromosom v kletkakh kostnogo mozga obez'ian posle prolongirovannogo oblucheniia.

The effect of prolonged gamma-irradiation with doses of low intensity (3.87 microA/kg) was studied in the bone marrow cells of monkeys (Macaca rhesus). The cumulative dose was 214.14-221.88 mC/kg. Statistically significant differences in the frequency of chromosome aberrations and the percentage of polyploid bone marrow cells, as compared to the level of spontaneous aberrations, were observed during 42 months following the exposure.