A study on the antioxidant, anti-inflammatory, and xanthine oxidase inhibitory activity of the Artemisia vulgaris L. extract and its fractions.

ETHNOPHARMACOLOGICAL RELEVANCE: Vietnamese people use mugwort (Artemisia vulgaris L.) to treat arthritis and gout. Our previous research shows that mugwort contains flavonoids, and its extract possesses antibacterial and anti-inflammatory activities. However, no publications have been on the xanthine oxidase inhibitory activity of mugwort and acute anti-inflammatory activity in vivo. AIM OF THE STUDY: The study aimed to verify the antioxidant, xanthine oxidase inhibitory, and anti-inflammatory capabilities of mugwort extract in vitro and in vivo, isolate phyto-compounds from potential bioactive fractions, and then evaluate their potential in inhibiting xanthine oxidase. METHODS: According to established methods, the extract and the active flavonoids were obtained using different chromatographic techniques. DPPH, ABTS, reducing power, and H2O2 elimination were used to evaluate antioxidant activity. The model of LPS-induced RAW264.7 cells was used to measure the inhibition of NO production. The carrageenan-induced paw oedema model was used to assess acute inflammation in mice. In vitro, xanthine oxidase inhibition assay was applied to investigate the effects of extract/compounds on uric acid production. Chemical structures were identified by spectral analysis. RESULTS: The assessment of the acute inflammatory model in mice revealed that both the 96% ethanol and the 50% ethanol extracts significantly decreased oedema in the mice's feet following carrageenan-induced inflammation. 96% ethanol extract exhibited a better reduction in oedema at the low dose. The analysis revealed that the ethyl acetate fraction had the highest levels of total polyphenols and flavonoids. Additionally, this fraction demonstrated significant antioxidant activity in various assays, such as DPPH, ABTS, reducing power, and H2O2 removal. Furthermore, it displayed the most potent inhibition of xanthine oxidase, an anti-inflammatory activity. Five phytochemicals were isolated and determined from the active fraction such as luteolin (1), rutin (2), apigenin (3), myricetin (4), and quercetin (5). Except for rutin, the other compounds demonstrated the ability to inhibit effective xanthine oxidase compared to standard (allopurinol). Moreover, quercetin (5) inhibited NO production (IC50 21.87 µM). CONCLUSION: The results indicate that extracts from A. vulgaris effectively suppressed the activity of xanthine oxidase and exhibited antioxidant and anti-inflammatory properties, potentially leading to a reduction in the production of uric acid in the body and eliminating ROS. The study identified mugwort extract and bioactive compounds derived from Artemisia vulgaris, specifically luteolin, apigenin, and quercetin, as promising xanthine oxidase inhibitors. These findings suggest that further development of these compounds is warranted. At the same time, the above results also strengthen the use of mugwort to treat gout disease in Vietnam.

Hydrological impacts of future climate and land use/cover changes in the Lower Mekong Basin: a case study of the Srepok River Basin, Vietnam.

This study presents hydrological impacts of future climate change (CC) and land use/cover change (LUCC) for the Srepok River Basin (SRB) in the Vietnam's Central Highlands. The hydrology cycle of this basin was reproduced using Soil and Water Assessment Tool (SWAT) allowing an evaluation of hydrological responses to CC and LUCC. Future climate scenarios of the 2015-2100 period under Representative Concentration Pathways (RCP) 4.5 simulated by five General Circulation Models (GCMs) and LUCC scenario in 2050 were developed. Compared to the reference scenario (1980-2005), future LUCC increases the streamflow (0.25%) and surface runoff (1.2%) and reduces the groundwater discharge (2.1%). Climate change may cause upward trends in streamflow (0.1 to 2.7%), surface runoff (0.4 to 4.3%), and evapotranspiration (0.8 to 3%), and a change in the groundwater discharge (- 1.7 to 0.1%). The combination of CC and LUCC increases the streamflow (0.2 to 2.8%), surface runoff (1.6 to 5.6%), and evapotranspiration (1.0 to 3.1%), and reduces the groundwater discharge (1.5 to 2.7%) with respect to the reference scenario. Moreover, the results noted that the water scarcity may happen in the dry-seasonal months.

Comparative Study of the Effect of Snake Venoms on the Growth of Ciliates Tetrahymena pyriformis: Identification of Venoms with High Antiprotozoal Activity.

To search for compounds with antiprotozoal activity, effects of snake venoms on the ciliates Tetrahymena pyriformis was studied. T. pyriformis from subkingdom of Protozoa, including the protozoal pathogens, was used as a model organism to select the venoms that are the most active against parasitic protozoans. Various concentrations of venoms were added to the cells, and the cells that survived after 24 h were counted. Among the six snake species from the Viperidae family, the venom of the viper Vipera berus, which completely killed the cells at 49 µg/mL, was the most active. Among four species from the Elapidae family, the previously studied cobra venoms containing cytotoxins with strong antiprotozoal activity as well as the venom of krait Bungarus multicinctus (10 µg/mL) were the most active. The venoms of the pit vipers and Nikolsky's viper did not show any activity at 12.5 mg/mL. Thus, the venoms of V. berus and B. multicinctus are promising for the isolation of new antiprotozoal compounds.

New Plant Species Showing Antiprotozoian Activity.

The effects of extracts of ten plant species from Russia and five species from Vietnam on the growth and survival of ciliates Tetrahymena pyriformis were studied. T. pyriformis belongs to the subkingdom Protozoa, which also includes pathogens of protozoan infections. Extraction of dried plants was carried out with acidic and alkaline aqueous solutions, as well as with an aqueous ethanol. Various amounts of extracts were added to the ciliate cells, and the number of cells survived after incubation for 1 and 24 h was recorded. We found that our samples of several plants, including wormwood, harmala, and licorice, similarly to those studied earlier, exhibit antiprotozoal activity, which may indicate that the secondary metabolites are the same in plants from different regions. Using the ciliate T. pyriformis as a model organism, the presence of antiprotozoal activity in extracts of lilac, chondrilla, cinquefoil, hop, and elm was shown for the first time.

The effects of climate variability and land-use change on streamflow and nutrient loadings in the Sesan, Sekong, and Srepok (3S) River Basin of the Lower Mekong Basin.

This paper aimed at examining the climate variability and land-use change effects on streamflow and pollutant loadings, namely total suspended sediment (TSS), total nitrogen (T-N), and total phosphorus (T-P), in the Sesan, Sekong, and Srepok (3S) River Basin in the period 1981-2010. The well-calibrated and validated Soil and Water Assessment Tool (SWAT) was used for this purpose. Compared to the reference period, climate variability was found to be responsible to a 1.00% increase in streamflow, 2.91% increase in TSS loading, 11.35% increase in T-N loading, and 19.12% reduction in T-P loading for the whole basin. With regard to the effect of land-use change (LUC), streamflow, TSS, T-N, and T-P loadings increased by 0.01%, 3.70%, 10.12%, and 10.94%, respectively. Therefore, the combination of climate variability and LUC showed amplified increases in streamflow (1.03%), TSS loading (7.09%), and T-N loading (25.05%), and a net effect of decreased T-P loading (10.35%). Regarding the Sekong and Srepok River Basins, the streamflow, TSS, T-N and T-P showed stronger responses to climate variability compared to LUC. In case of the Sesan River Basin, LUC had an effect on water quantity and quality more strongly than the climate variability. In general, the findings of this work play an essential role in providing scientific information to effectively support decision makers in developing sustainable water resources management strategies in the study area.

Therapeutic efficacy and safety of chamomile for state anxiety, generalized anxiety disorder, insomnia, and sleep quality: A systematic review and meta-analysis of randomized trials and quasi-randomized trials.

This systematic review and meta-analysis aimed to study the efficacy and safety of chamomile for the treatment of state anxiety, generalized anxiety disorders (GADs), sleep quality, and insomnia in human. Eleven databases including PubMed, Science Direct, Cochrane Central, and Scopus were searched to retrieve relevant randomized control trials (RCTs), and 12 RCTs were included. Random effect meta-analysis was performed by meta package of R statistical software version 3.4.3 and RevMan version 5.3. Our meta-analysis of three RCTs did not show any difference in case of anxiety (standardized mean difference = -0.15, 95% CI [-0.46, 0.16], P = 0.4214). Moreover, there is only one RCT that evaluated the effect of chamomile on insomnia and it found no significant change in insomnia severity index (P > 0.05). By using HAM-A scale, there was a significant improvement in GAD after 2 and 4 weeks of treatment (mean difference = -1.43, 95% CI [-2.47, -0.39], P = 0.007), (MD = -1.79, 95% CI [-3.14, -0.43], P = 0.0097), respectively. Noteworthy, our meta-analysis showed a significant improvement in sleep quality after chamomile administration (standardized mean difference = -0.73, 95% CI [-1.23, -0.23], P < 0.005). Mild adverse events were only reported by three RCTs. Chamomile appears to be efficacious and safe for sleep quality and GAD. Little evidence is there to show its effect on anxiety and insomnia. Larger RCTs are needed to ascertain these findings.

Low-molecular-weight compounds with anticoagulant activity from the scorpion Heterometrus laoticus venom.

Low-molecular-weight compounds with anticoagulant activity were isolated from the scorpion Heterometrus laoticus venom. The determination of the structure of the isolated compounds by nuclear magnetic resonance and mass spectrometry showed that one of the isolated compounds is adenosine, and the other two are dipeptides leucyl-tryptophan and isoleucyl-tryptophan. The anticoagulant properties of adenosine, which is an inhibitor of platelet aggregation, is well known, but its presence in scorpion venom is shown for the first time. The ability of leucyl-tryptophan and isoleucyl-tryptophan to slow down blood clotting and their presence in scorpion venom are also established for the first time.

Evaluating the impacts of climate and land-use change on the hydrology and nutrient yield in a transboundary river basin: A case study in the 3S River Basin (Sekong, Sesan, and Srepok).

Assessment of the climate and land-use change impacts on the hydrology and water quality of a river basin is important for the development and management of water resources in the future. The objective of this study was to examine the impact of climate and land-use change on the hydrological regime and nutrient yield from the 3S River Basin (Sekong, Srepok, and Sesan) into the 3S River system in Southeast Asia. The 3S Rivers are important tributaries of the Lower Mekong River, accounting for 16% of its annual flow. This transboundary basin supports the livelihoods of nearly 3.5 million people in the countries of Laos, Vietnam, and Cambodia. To reach a better understanding of the process and fate of pollution (nutrient yield) as well as the hydrological regime, the Soil and Water Assessment Tool (SWAT) was used to simulate water quality and discharge in the 3S River Basin. Future scenarios were developed for three future periods: 2030s (2015-2039), 2060s (2045-2069), and 2090s (2075-2099), using an ensemble of five GCMs (General Circulation Model) simulations: (HadGEM2-AO, CanESM2, IPSL-CM5A-LR, CNRM-CM5, and MPI-ESM-MR), driven by the climate projection for RCPs (Representative Concentration Pathways): RCP4.5 (medium emission) and RCP8.5 (high emission) scenarios, and two land-use change scenarios. The results indicated that the climate in the study area would generally become warmer and wetter under both emission scenarios. Discharge and nutrient yield is predicted to increase in the wet season and decrease in the dry. Overall, the annual discharge and nutrient yield is projected to increase throughout the twenty-first century, suggesting sensitivity in the 3S River Basin to climate and land-use change. The results of this study can assist water resources managers and planners in developing water management strategies for uncertain climate change scenarios in the 3S River Basin.