RESUMEN
We report experimental results on the inactivation of encephalomyocarditis virus, M13 bacteriophage, and Salmonella typhimurium by a visible femtosecond laser. Our results suggest that inactivation of virus and bacterium by a visible femtosecond laser involves completely different mechanisms. Inactivation of viruses by a visible femtosecond laser involves the breaking of hydrogen∕hydrophobic bonds or the separation of the weak protein links in the protein shell of a viral particle. In contrast, inactivation of bacteria is related to the damage of their DNAs due to irradiation of a visible femtosecond laser. Possible mechanisms for the inactivation of viruses and bacteria are discussed.
Asunto(s)
Bacteriófago M13/efectos de la radiación , Virus de la Encefalomiocarditis/efectos de la radiación , Láseres de Estado Sólido/uso terapéutico , Salmonella typhimurium/efectos de la radiación , Animales , Bovinos , Dicroismo Circular , ADN Viral/efectos de la radiación , Microscopía de Fuerza Atómica , Microscopía de Fluorescencia por Excitación Multifotónica , Albúmina Sérica Bovina/química , Albúmina Sérica Bovina/efectos de la radiación , Espectrometría de Fluorescencia , Espectrometría Raman , Virión/efectos de la radiaciónRESUMEN
Raman spectroscopy is used to study low-wave-number (