RESUMEN
Lichens are well-known biomonitors for semi-volatile pollutants, due to their ability to absorb and retain different chemical compounds such as Polycyclic Aromatic Hydrocarbons (PAHs), directly linked to levels in the atmosphere. Based on that, this paper proposes an analytical method capable of quantifying 16 EPA-PAHs from lichens found in an intertropical zone, as a natural alternative to typical capture methods, with the aim of monitoring atmospheres polluted by toxic compounds. An analytical protocol, including sample pre-treatment, followed by ultrasound extraction, clean-up in a chromatographic column, concentration and quantification by Gas Chromatography-Mass Spectrometry (GC-MS) using Selective Ion Monitoring has been developed. Additionally, a set of guidelines on lichen collection and sample handling is given, in order to achieve representative samples.â¢Limits of quantification (LOQ) and detection (LOD) varied from 2.0 to 16 µg/L and 1.0 to 5.0 µg/L, respectively. Calibration curves had correlation coefficients higher than 0.99 in all cases.â¢Validation of the method for determining PAHs concentration associated to 30 lichen samples collected along two roads, with high and low traffic volumes was carried out.â¢The method showed good performance according to the sources of PAHs, traffic patterns and gradient in roads.