[THE DEVICE REGISTRATION OF IMMUNE CHROMATOGRAPHIC EXPRESS-TESTS].

The development of sector of "fast-testing" i.e. test-systems permitting carrying out analysis during home visit of doctor or at primary examination of patient without any additional devices and reagents is predominant tendency in international practice. The immunochromatography is an effective technical solution in out-laboratory diagnostic, which nowadays is actively applied in controlling hundreds of diagnostically significant markers of infectious diseases, metabolic and functional disorders. However, common immunochromatography is focused on qualitative visual evaluation of results of study i.e. conclusion on presence or absence of coloration of particular zones of test-band. Therefore, the technical solutions retaining such merits of immunochromatography as expressness and technical simplicity and at the same time providing objectivity of diagnostic and increasing its informativeness are extremely in demand. The review considers main methodical solutions and tendencies of their practical implementation targeted at device documentation, processing and interpretation of results of immunochromatography analysis. The optical systems of registration in visible area of spectrum dominating in assortment of modern detectors are presented. The new solutions oriented on working with fluorescent, magnetic and electroconductive markers are presented too. The perspectives of further development of this direction are characterized including application as detectors of domestic communication devices and formation of cloud data bases for storage and processing of information concerning results of examinations.

Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling.

Nanodispersed gold is widely used as a marker in different analytical systems. For such purposes, it is usually obtained by the reduction of salts. This work studied the potential analytical applications of nanodispersed gold obtained by laser ablation because gold produced with this method has no chemical coating. The nanoparticles produced were characterized by transmission electron microscopy and spectrophotometry. The average size of the particles was 24.5 nm. Concentration dependences of antibody immobilization on ablative gold were obtained. With the use of antibody-conjugated nanoparticles, an immunochromatographic system was constructed for the detection of zearalenone mycotoxin. This immunoassay was characterized by a detection limit of 0.1 ng/ml antigen with an assay duration of only 15 min, which is on par with current test systems comprising nanodispersed gold obtained by chemical reduction. The simplicity of ablative dispersing makes this a prospective method for the labeling of various antibodies for analytical use.

[Immunochemical methods of mycotoxin analysis (review)].

The review is devoted to comparative characterization of immunochemical methods of detection of mycotoxin, which belongs to one of the priority groups of the food contaminants. It has been shown that the high specificity and the possibility of mycotoxin detection in low concentrations combined with existent diverse equipment allow for considering the immunochemical methods of analysis to be the most promising for wide practical application. The analytical characteristics of the existent developments are presented; the merits and demerits of the different kinds of immunoanalytical systems are compared.