Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.

Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel. Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence. Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures. Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared. Grains were tempered with added moisture (0%, 3%, or 6%) prior to steam conditioning for 15, 30, or 45 min. No two- or three-way interactions were observed. Adding moisture improved starch availability (linear; P < 0.01), and tended to improve ISDMD (linear, P = 0.06). Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min. Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01). Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively. Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen). Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01). Hot carcass weight was 6 kg greater for EFC cattle (P <0.01) than CON. No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13). Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33). Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03). High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.

Effects of quality grade and intramuscular location on beef semitendinosus muscle fiber characteristics, NADH content, and color stability.

The objective of this study was to determine the impact of quality grade and steak location on color stability of semitendinosus (ST) steaks during a 9-d refrigerated study. Twenty-one ST muscles (12 Choice and 9 Select) were purchased from a commercial beef packing plant and fabricated into twelve 2.54-cm thick steaks per muscle. Steaks 1, 6, and 12 were designated for immunohistochemistry while remaining steak locations of proximal (steaks 2 to 4), middle (5 to 8), and distal (9 to 11) were randomly assigned to 0, 4, or 9 d of simulated retail display. Surface color attributes of day-9 steaks were recorded daily by a visual color panel and spectrophotometer. On days 0, 4, and 9 of display, steaks were analyzed for metmyoglobin reducing ability (MRA) and oxygen consumption (OC). Grade × day of display (DOD) interactions were detected for L*, a*, surface oxymyoglobin (OMb) and metmyoglobin (MMb) percentages, and visual panel surface redness and discoloration scores (P ≤ 0.02); however, no Grade × DOD interactions were observed for MRA or OC (P > 0.17). There were location main effect (LOC) × DOD interactions for L*, a*, surface MMb, visual panel surface redness and discoloration, and MRA (P ≤ 0.02). Distal steaks had lower L* values compared with the other locations (P < 0.01), which coincided with steaks being rated visually darker red (P < 0.01). Proximal steaks had greater a* values and had less surface discoloration than middle steaks (P < 0.05), which had an increased percentage of surface MMb (P ≤ 0.04). Distal and proximal steaks had increased MRA compared with middle steaks on days 0 and 4 (P < 0.05), and distal steaks had greater OC than the other locations throughout display (P < 0.01). There were fewer type I fibers at the proximal end with a greater percentage located at the middle and distal ends, and an increased percentage of type IIX fibers at the middle and proximal locations (P ≤ 0.01). Less type IIA fibers were detected at the middle LOC compared with the other two locations (P < 0.10). Larger type I, IIA, and IIX fibers were located at the proximal and middle locations compared with the distal LOC (P < 0.01). ST color and color-stability characteristics were influenced by DOD and LOC, which may partially be explained by differences in fiber types among locations.

Elevated concentrations of crude glycerin in diets for beef cattle: feedlot performance, carcass traits, and ruminal metabolism1.

Three experiments were conducted to evaluate the inclusion of crude glycerin (CG) in diets for beef cattle. In Exp. 1, 4 ruminally cannulated steers were fed diets with 0 or 15% CG (DM basis), to evaluate DM disappearance, VFA profiles, and gas production. There was a tendency for an interaction (P = 0.06) between diet fed to donor animals and substrate fed to in vitro system, and digestion was increased when CG was added to cultures with ruminal fluid from CG-fed animals. Total VFA were unaffected by diets or by substrate incubated. The CG increased production of propionate, butyrate, and valerate (P < 0.01) while the gas production was unaffected (P = 0.16). In Exp. 2, 24 crossbred heifers (334.4 ± 0.9 kg BW) were fed the same diets as Exp. 1, for 35 d. Fecal grab samples were collected 3 times daily on day 7, 21, and 35, to evaluate total tract digestibilities of DM, OM, and NDF. The CG improved digestibility of diet OM (P = 0.04), and DM followed a similar trend (P = 0.06), while the NDF digestibility was unaffected (P = 0.29). In Exp. 3, crossbred heifers (n = 374; 375.8 ± 36.1 kg BW) were used to evaluate feedlot performance and carcass traits when fed diets with 0, 7.5, or 15% CG, with or without added 0.3% salt. Heifers were assigned to 25 pens and were harvested on day 125. Removing salt from CG-based diets did not impact performance (P = 0.50). The CG did not influence average daily gain (P = 0.27), but decreased DM intake (P = 0.003), USDA Yield Grade (P = 0.01), and improved feed efficiency (P = 0.03), while tended to decrease USDA prime carcasses (P = 0.10). Carcass weight (P = 0.24), Longissimus muscle area (P = 0.63), and kidney, pelvic, heart fat (P = 0.59) were unaffected by CG. Twelfth-rib fat was less for heifers fed 15% CG without salt compared with the other treatments (P = 0.005), while marbling was less for heifers fed CG diets compared with the control-fed animals (linear, P = 0.004; quadratic, P = 0.02). In conclusion, GC can replace dry-rolled corn in diets for beef heifers when fed at 15% of diet DM, improving OM digestion, increasing ruminal propionate and butyrate without affecting greenhouse gas emissions. Feeding up to 15% CG improves feed efficiency but depresses marbling and tends to decrease Quality Grade. Removing supplemental salt from CG-diets has no impact on performance or carcass traits.

Effects of supplemental zinc sulfate on growth performance, carcass characteristics, and antimicrobial resistance in feedlot heifers.

Effects of supplemental Zn as Zn sulfate on feedlot performance, carcass traits, and antimicrobial resistance were evaluated using 480 crossbred heifers (BW = 385 kg ± 13.08) in a randomized complete block design. Heifers were blocked by BW and randomly assigned within block to diets with 0, 30, 60, or 90 mg supplemental Zn/kg DM. Heifers were housed in dirt-surfaced pens (20 animals per pen; 6 pens per treatment) equipped with fence-line feed bunks and automatic water fountains. Heifers were fed once daily to ensure ad libitum intake. Plasma was collected on day 0 from five randomly selected heifers per pen and repeated on days 63 and 115 to determine plasma Zn concentrations. Random samples of freshly voided feces were collected from the surface of each pen the day of harvest to determine antibiotic resistance. Heifers were transported on day 144 to a commercial abattoir where hot carcass weight (HCW) and incidence of liver abscesses were recorded at harvest and HCW, dressed yield, ribeye area, 12th rib fat, quality and yield grades were recorded after 36 h of refrigeration. Plasma Zn concentration increased (P = 0.02) linearly in response to increasing concentrations of dietary Zn. Final BW and ADG were unaffected by supplementation (P ≥ 0.29). Quantified levels of resistance to ceftriaxone and tetracycline among fecal Escherichia coli were not impacted (P > 0.05) by dietary zinc concentrations. Increasing Zn concentrations tended to decrease (linear effect, P = 0.07) DMI, resulting in a linear (P = 0.03) and tendency for quadratic (P = 0.12) improvement in feed efficiency with increasing Zn concentration. No differences were detected for HCW, dressed yield, ribeye area, 12th rib fat, percentages of carcasses grading Select or Choice, or yield grade (P > 0.53), but added Zn tended to affect percentage of carcasses that graded Prime, peaking at 60 mg/kg added Zn (quadratic effect, P = 0.07). In vitro fermentations were performed using ruminal fluid cultures containing 0, 30, 60, 90, 120, or 150 mg Zn/kg substrate DM to determine impact of Zn on gas production, VFA concentrations, and in vitro DM disappearance (IVDMD). There were no effects of Zn on in vitro gas production, IVDMD, or most VFA (P > 0.15), but isovalerate decreased linearly in response to added Zn (P = 0.05). Supplementing finishing heifers up to 60 mg Zn/kg diet DM improved feed efficiency compared to other treatments.

Effect of alpha-tocopherol acetate and ascorbic acid on performance, carcass traits, and incidence and severity of liver abscesses in feedlot cattle.

Liver abscesses (LA) in cattle negatively affect feedlot performance by decreasing ADG, feed intake, and G:F ratio. Abscessed livers are condemned and abdominal adhesions associated with LA can result in extensive carcass trimming during harvest, further compounding adverse economic impact. Given regulatory changes pertaining to the use of in-feed antibiotics in cattle production, there is growing interest in alternatives to antibiotics for LA control. The objective of this study was to evaluate the use of antioxidants, crystalline ascorbate (AOX), and alpha-tocopherol acetate, for mitigation of LA in feedlot cattle. Yearling crossbred heifers (n = 392; initial BW 481 ± 9.4 kg) were blocked by previous treatment and allocated randomly to 24 dirt-surfaced feedlot pens (10 m × 35 m) with 14 heifers/pen. Heifers were weighed, implanted with Component TE-200 implants, and placed into feeding pens. Finishing diets consisted of 60% steam-flaked corn, 30% wet corn gluten feed, 8% alfalfa, and 2% supplement (DM basis) that provided 300 mg/d monensin, and either 200 IU/d alpha-tocopherol acetate (CTL) or 2,000 IU/d alpha-tocopherol acetate plus 500 mg/d crystalline AOX. Heifers were fed once daily ad libitum for 94 d, then weighed and transported 450 km to a commercial abattoir for harvest. Hot carcass weight and incidence/severity of LA were determined the day of harvest, and carcass traits were evaluated following 36 h of refrigeration. Compared to CTL, feeding AOX tended to decrease DMI (10.66 vs. 10.31 kg/d; P = 0.08) and improve G:F (0.1204 vs. 0.1254; P = 0.12), but did not impact ADG, incidence of LA (25.6 vs. 23.5% for CTL and AOX, respectively), HCW (828.4 vs. 830.5 kg for CTL and AOX, respectively), or other carcass traits (P > 0.20). In conclusion, feeding antioxidants are not a viable alternative to decrease incidence of LA in finishing cattle.

Effects of intermittent feeding of tylosin phosphate during the finishing period on feedlot performance, carcass characteristics, antimicrobial resistance, and incidence and severity of liver abscesses in steers.

Liver abscesses (LA) are a source of economic loss for feedlot cattle feedlots, and the 2017 veterinary feed directive has restricted further use of tylosin phosphate to prevention and control of LA. Our objective was to evaluate effects of intermittent tylosin phosphate feeding on incidence and severity of LA in feedlot cattle and presence of total antimicrobial-resistant Enterococcus spp. Steers (n = 312, 411.4 ± 6.71 kg) were blocked by initial BW and randomly assigned to a treatment group. Treatments included a negative control group (no tylosin phosphate throughout the finishing period), a positive control group (tylosin phosphate fed continuously throughout the finishing period), and a group that received tylosin phosphate off-label by feeding the drug on a repeated intermittent basis (1 wk on, 2 wk off). Steers were housed in 24 soil-surfaced pens with 13 steers per pen. Body weights of cattle were obtained every 28 d and at the end of 119 d the steers were weighed and harvested at a commercial abattoir. Fecal samples were collected on days 0, 21, and 118 to characterize antimicrobial-resistant Enterococcus spp. Total LA percentage was greater (P = 0.012) for the no tylosin phosphate treatment compared with the other treatments, but did not differ between the continuous tylosin phosphate treatment and the intermittently fed tylosin phosphate treatment (P = 0.716). No difference was observed among treatments for ADG (P = 0.21), DMI (P = 0.28), or G:F (P = 0.75). Marbling score was lower (P = 0.022) for tylosin phosphate treatment when compared with both intermittent treatment and continuous tylosin phosphate treatment. Enterococcus spp. bacterial counts did not differ by treatment group over time (P > 0.05); however, there was a strong period effect for macrolide resistance among all groups (P < 0.01), suggesting an important environmental component as cattle were first placed in pens and then progressed through the feeding period. We conclude that feeding tylosin phosphate intermittently during the finishing phase decreases the total percentage of LA and maintains feedlot performance and carcass characteristics to the same extent as feeding tylosin phosphate throughout the finishing phase; furthermore, we hypothesize that enteric antimicrobial resistance is a result of longer term antibiotic usage in a particular environment rather than a direct short-term result of the treatment during any given feeding period.