RESUMEN
An emerging therapeutic approach for Duchenne muscular dystrophy is the transplantation of autologous myogenic progenitor cells genetically modified to express dystrophin. The use of this approach is challenged by the difficulty in maintaining these cells ex vivo while keeping their myogenic potential, and ensuring sufficient transgene expression following their transplantation and myogenic differentiation in vivo. We investigated the use of the piggyBac transposon system to achieve stable gene expression when transferred to cultured mesoangioblasts and into murine muscles. Without selection, up to 8% of the mesoangioblasts expressed the transgene from 1 to 2 genomic copies of the piggyBac vector. Integration occurred mostly in intergenic genomic DNA and transgene expression was stable in vitro. Intramuscular transplantation of mouse Tibialis anterior muscles with mesoangioblasts containing the transposon led to sustained myofiber GFP expression in vivo. In contrast, the direct electroporation of the transposon-donor plasmids in the mouse Tibialis muscles in vivo did not lead to sustained transgene expression despite molecular evidence of piggyBac transposition in vivo. Together these findings provide a proof-of-principle that piggyBac transposon may be considered for mesoangioblast cell-based therapies of muscular dystrophies.
Asunto(s)
Tratamiento Basado en Trasplante de Células y Tejidos/métodos , Cromosomas Artificiales Bacterianos/metabolismo , Técnicas de Transferencia de Gen , Músculo Esquelético/metabolismo , Animales , Diferenciación Celular , Células Cultivadas , Cromosomas Artificiales Bacterianos/genética , Femenino , Vectores Genéticos/genética , Vectores Genéticos/metabolismo , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Ratones , Ratones Endogámicos C57BL , Ratones Desnudos , Microscopía Fluorescente , Músculo Esquelético/citología , Músculo Esquelético/patología , Distrofia Muscular de Duchenne/patologíaRESUMEN
AIM: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. MATERIALS & METHODS: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patients and healthy volunteers, as well as mice models, and Young's modulus stiffness values were determined. RESULTS: Fibers displaying abnormally low mechanical stability were detected in biopsies from patients affected by 11 distinct muscle diseases, and Young's modulus values were commensurate to the severity of the disease. Abnormal myofiber resistance was also observed from consulting patients whose muscle condition could not be detected or unambiguously diagnosed otherwise. DISCUSSION & CONCLUSION: This study provides a proof-of-concept that atomic force microscopy yields a quantitative read-out of human muscle function from clinical biopsies, and that it may thereby complement current muscular dystrophy diagnosis.
Asunto(s)
Microscopía de Fuerza Atómica/métodos , Músculo Esquelético/patología , Distrofias Musculares/patología , Adolescente , Adulto , Anciano , Animales , Fenómenos Biomecánicos , Niño , Módulo de Elasticidad , Femenino , Humanos , Masculino , RatonesRESUMEN
BACKGROUND: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. RESULTS: In this study, we devised vectors allowing a tight regulation of transgene expression in mice from such non-viral vectors using a doxycycline-controlled network of activator and repressor proteins. Using these vectors, we demonstrate proper physiological response as consequence of the induced expression of two therapeutically relevant proteins, namely erythropoietin and utrophin. Kinetic studies showed that the induction of transgene expression was only transient, unless epigenetic regulatory elements termed Matrix Attachment Regions, or MAR, were inserted upstream of the regulated promoters. Using episomal plasmid rescue and quantitative PCR assays, we observed that similar amounts of plasmids remained in muscles after electrotransfer with or without MAR elements, but that a significant portion had integrated into the muscle fiber chromosomes. Interestingly, the MAR elements were found to promote plasmid genomic integration but to oppose silencing effects in vivo, thereby mediating long-term expression. CONCLUSIONS: This study thus elucidates some of the determinants of transient or sustained expression from the use of non-viral regulated vectors in vivo.
Asunto(s)
Regulación de la Expresión Génica , Técnicas de Transferencia de Gen , Vectores Genéticos , Regiones de Fijación a la Matriz , Plásmidos/genética , Transgenes , Animales , Doxiciclina/farmacología , Epigénesis Genética , Eritropoyetina/genética , Eritropoyetina/metabolismo , Femenino , Humanos , Cinética , Ratones , Ratones Endogámicos C57BL , Modelos Animales , Músculos/metabolismo , Utrofina/genética , Utrofina/metabolismoRESUMEN
To date two closely related interleukin-1ß genes (IL-1ß1 and IL-ß2) have been found in salmonids. The cloning of trout and salmon IL-1ß3, and a salmon IL-1ß4 pseudogene reveals that two types of IL-1ß genes exist in teleost species. Type I teleost IL-1ß genes, including salmonid IL-1ß3, share a similar 6 coding exon structure as in tetrapods. Type II teleost IL-1ß genes, e.g. salmonid IL-1ß1-2, lack one or two coding exons at their 5'-end, and share higher identities within this subgroup than within the type I subgroup. Both types of IL-1ß genes have been found in species of Salmoniformes, Perciformes and Beloniformes, suggesting the divergence occurred early in teleost evolution. Trout IL-1ß3 is highly expressed in ovary suggesting a role in reproduction. A relatively high constitutive expression in gills, spleen and kidney and the up-regulation by PAMPs, proinflammatory cytokines and viral infection suggests IL-1ß3 also has a role in inflammation and host defence.