Changes in the abundance and diversity of bacterial and archaeal communities at different depths in a eutrophic freshwater lake in southwestern Mexico.

Bacteria and archaea play a fundamental role in the biogeochemical cycles of organic matter, pollutants, and nutrients to maintain the trophic state of aquatic ecosystems. However, very little is known about the composition patterns of microbial communities in vertical distribution (water column) in freshwater lakes and their relationship with the physicochemical properties of water. "La Encantada" lake in the Lagunas de Montebello National Park (LMNP) is a site of interest due to the anthropogenic impact received and the little information about it. In this study, 3 sites were evaluated; samples were collected using 0-15 m deep water columns and analyzed using Illumina MiSeq sequencing technology based on the 16S rRNA gene. The physical parameters of pH, temperature, dissolved oxygen, electrolytic conductivity, and PO-4 were determined. The results revealed clear differences in the microbial composition of the water throughout the column; the most abundant phyla in bacterial communities were Proteobacteria (23.2%), Cyanobacteria (17.3%), and Bacteroidetes (17.2%), and for archaea were Crenarchaeota (35.9%) and Euryarchaeota (33.2%). PICRUSt metabolic inference analysis revealed that the main functional genes were related to cellular processes and biodegradation of xenobiotics, indicating an increasing trend of contaminants and residual discharges that may act as a precursor to alter microbial communities and stability of the lakes. At depths of 10 and 15 m, the microbial diversity was greater; likewise, the correlation between the physicochemical parameters and the microbial communities at the genus level showed that Chlorobaculum, Desulfomonile, and Candidatus Xiphinematobacter were favored by an increase in dissolved phosphates and by the decrease in pH and temperature. These results highlight that the microbial communities exhibit variation in their composition due to the effect of depth and physicochemical parameters, which could play a role as biological factors in the trophic states of a lake.

Prokaryotic diversity across a pH gradient in the "El Chichón" crater-lake: a naturally thermo-acidic environment.

The "El Chichón" crater-lake in Mexico is a thermo-acidic environment whose microorganisms have been scarcely studied. In this study, we surveyed the prokaryotic communities by amplicon sequencing of the 16S rRNA gene considering samples of sediment and water collected within a pH/temperature gradient (pH 1.9-5.1, 38-89 °C). Further, we interpreted these results within a physicochemical context. The composition of the microbial assemblage differed significantly between the sediments and water. Sediment communities were different in the site with the highest temperature and lower pH value compared to the other ones sampled, while those in the water were relatively similar at all points. Most of the genera found were related to Alicyclobacillus, Acinetobacter, Bacillus, Mesoaciditoga, Methanothermobacter, Desulfitobacterium, Therminicanus, Kyrpidia, Paenibacillus, Thermoanaerobacterium, and Gelria. Some of these genera are known by their thermo-acidic tolerant capacities with flexible metabolisms to use diverse electron donor/acceptors (S or Fe), while others are thermo(acid)philes that mainly occur in the most extreme sites of the lake. These results show the presence of a microbial community adapted to the changing conditions of a very dynamic crater-lake, that include chemoorganotrophs and chemolithotrophs.

Evaluation of temperature, pH and nutrient conditions in bacterial growth and extracellular hydrolytic activities of two Alicyclobacillus spp. strains.

Extremophile bacteria have developed the metabolic machinery for living in extreme temperatures, pH, and high-salt content. Two novel bacterium strains Alicyclobacillus sp. PA1 and Alicyclobacillus sp. PA2, were isolated from crater lake El Chichon in Chiapas, Mexico. Phylogenetic tree analysis based on the 16SrRNA gene sequence revealed that the strain Alicyclobacillus sp. PA1 and Alicyclobacillus sp. PA2 were closely related to Alicyclobacillus species (98% identity and 94.73% identity, respectively). Both strains were Gram variable, and colonies were circular, smooth and creamy. Electron microscopy showed than Alicyclobacillus sp. PA1 has a daisy-like form and Alicyclobacillus sp. PA2 is a regular rod. Both strains can use diverse carbohydrates and triglycerides as carbon source and they also can use organic and inorganic nitrogen source. But, the two strains can grow without any carbon or nitrogen sources in the culture medium. Temperature, pH and nutrition condition affect bacterial growth. Maximum growth was produced at 65 °C for Alicyclobacillus sp. PA1 (0.732 DO600) at pH 3 and Alicyclobacillus sp. PA2 (0.725 DO600) at pH 5. Inducible extracellular extremozyme activities were determined for ß-galactosidase (Alicyclobacillus sp. PA1: 88.07 ± 0.252 U/mg, Alicyclobacillus sp. PA2: 51.57 ± 0.308 U/mg), cellulose (Alicyclobacillus sp. PA1: 141.20 ± 0.585 U/mg, Alicyclobacillus sp. PA2: 51.57 ± 0.308 U/mg), lipase (Alicyclobacillus sp. PA1: 138.25 ± 0.600 U/mg, Alicyclobacillus sp. PA2: 175.75 ± 1.387 U/mg), xylanase (Alicyclobacillus sp. PA1: 174.72 ± 1.746 U/mg, Alicyclobacillus sp. PA2: 172.69 ± 0.855U/mg), and protease (Alicyclobacillus sp. PA1: 15.12 ± 0.121 U/mg, Alicyclobacillus sp. PA2: 15.33 ± 0.284 U/mg). These results provide new insights on extreme enzymatic production on Alicyclobacillus species.