RESUMEN
Using electrophysiology, the effect of nicotinic acetylcholine receptor (nAChR) ligands on acetylcholine-induced depolarization in the neurons of Helix lucorum snail was studied. It was found that the α-conotoxin PnIA [R9, L10], a selective antagonist of α7 nAChR, and α-cobratoxin (antagonist of α7 and muscle-type nAChR) suppressed neuronal depolarization. Fluorescence microscopy showed staining of the neurons with fluorescently labeled α-bungarotoxin; this staining was reduced by pretreatment with α-cobratoxin. Induced depolarization was also suppressed by α-conotoxin RgIA, a selective inhibitor of α9 nAChR. In contrast to Lymnaea stagnalis nAChR, which are weakly sensitive to neurotoxin II and α-conotoxin GI, antagonists of muscle-type nAChR, H. lucorum receptors were most effectively inhibited by these antagonists. The results obtained, as well as the previously found sensitivity of the receptors studied in this work to muscarinic receptor ligands, indicate an unusual atypical pharmacological profile of H. lucorum nAChR.
Asunto(s)
Neuronas/metabolismo , Receptores Colinérgicos/metabolismo , Receptor Nicotínico de Acetilcolina alfa 7/metabolismo , Acetilcolina/metabolismo , Animales , Sitios de Unión , Compuestos Bicíclicos Heterocíclicos con Puentes/farmacología , Bungarotoxinas/metabolismo , Caracoles Helix , Ligandos , Microscopía Confocal , Microscopía Fluorescente , Neurotoxinas/metabolismo , Piridinas/farmacología , Transducción de SeñalRESUMEN
The influence of femtosecond laser pulses on the proliferative activity of HaCaT keratinocytes and mesenchymal stromal cells (MSC) rats was studied. The growth media was exposed by laser pulses with wavelength 590 nm and duration 30 fs. The dependence of proliferative activity of cells on the dose was showed in the range 6-4299 J/cm2. Proliferative activity was assessed by the number of cells after 1 day after exposure. For both cell cultures obtained similar dose dependence: an increase in cell proliferation (32-54% for HaCaT and 19% for MSK) occurs when using lower doses, while higher doses no changes the rate of proliferation of cells. Conducted physical and chemical analysis found no increase in the concentration of active forms of oxygen in the culture medium. The impact of femtosecond laser pulses has led to the generation in culture medium acoustic oscillations in the range of 0.5 to 6.0 kHz. It is assumed that the increase in proliferative activity of cells, can be caused by mechanical effects of acoustic waves generated in the environment of optical breakdown in the focus of the laser radiation.
