RESUMEN
The target of rapamycin (TOR) kinase pathway regulates various biological processes, including translation, synthesis of ribosomal proteins, and transcription of rRNA. The ribosomal protein S6 (RPS6) is one of the well known downstream components of the TOR pathway. Ribosomal proteins have been known to have diverse functions in regulating cellular metabolism as well as protein synthesis. So far, however, little is known about other possible role(s) of RPS6 in plants, besides being a component of the 40 S ribosomal subunit and acting as a target of TOR. Here, we report that RPS6 may have a novel function via interaction with histone deacetylase 2B (AtHD2B) that belongs to the plant-specific histone deacetylase HD2 family. RPS6 and AtHD2B were localized to the nucleolus. Co-expression of RPS6 and AtHD2B caused a change in the location of both RPS6 and AtHD2B to one or several nucleolar spots. ChIP analysis suggests that RPS6 directly interacts with the rRNA gene promoter. Protoplasts overexpressing both AtHD2B and RPS6 exhibited down-regulation of pre-18 S rRNA synthesis with a concomitant decrease in transcription of some of the ribosomal proteins, suggesting their direct role in ribosome biogenesis and plant development. This is consistent with the mutation in rps6b that results in reduction in 18 S rRNA transcription and decreased root growth. We propose that the interaction between RPS6 and AtHD2B brings about a change in the chromatin structure of rDNA and thus plays an important role in linking TOR signaling to rDNA transcription and ribosome biogenesis in plants.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Nucléolo Celular/metabolismo , Genes de Plantas/fisiología , Genes de ARNr/fisiología , Fosfatidilinositol 3-Quinasas/metabolismo , ARN de Planta/metabolismo , ARN Ribosómico 18S/biosíntesis , Arabidopsis/citología , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Nucléolo Celular/genética , ADN de Plantas/genética , ADN de Plantas/metabolismo , ADN Ribosómico/genética , ADN Ribosómico/metabolismo , Epigénesis Genética/fisiología , Histona Desacetilasas/genética , Histona Desacetilasas/metabolismo , Fosfatidilinositol 3-Quinasas/genética , Regiones Promotoras Genéticas/fisiología , Protoplastos/citología , Protoplastos/metabolismo , ARN de Planta/genética , ARN Ribosómico 18S/genética , Transcripción Genética/fisiologíaRESUMEN
Legume-Rhizobium spp. symbiosis requires signaling between the symbiotic partners and differential expression of plant genes during nodule development. Previously, we cloned a gene encoding a putative ß-carotene hydroxylase (GmBCH1) from soybean (Glycine max) whose expression increased during nodulation with Bradyrhizobium japonicum. In this work, we extended our study to three GmBCHs to examine their possible role(s) in nodule development, as they were additionally identified as nodule specific, along with the completion of the soybean genome. In situ hybridization revealed the expression of three GmBCHs (GmBCH1, GmBCH2, and GmBCH3) in the infected cells of root nodules, and their enzymatic activities were confirmed by functional assays in Escherichia coli. Localization of GmBCHs by transfecting Arabidopsis (Arabidopsis thaliana) protoplasts with green fluorescent protein fusions and by electron microscopic immunogold detection in soybean nodules indicated that GmBCH2 and GmBCH3 were present in plastids, while GmBCH1 appeared to be cytosolic. RNA interference of the GmBCHs severely impaired nitrogen fixation as well as nodule development. Surprisingly, we failed to detect zeaxanthin, a product of GmBCH, or any other carotenoids in nodules. Therefore, we examined the possibility that most of the carotenoids in nodules are converted or cleaved to other compounds. We detected the expression of some carotenoid cleavage dioxygenases (GmCCDs) in wild-type nodules and also a reduced amount of zeaxanthin in GmCCD8-expressing E. coli, suggesting cleavage of the carotenoid. In view of these findings, we propose that carotenoids such as zeaxanthin synthesized in root nodules are cleaved by GmCCDs, and we discuss the possible roles of the carotenoid cleavage products in nodulation.
Asunto(s)
Glycine max/fisiología , Oxigenasas de Función Mixta/genética , Oxigenasas de Función Mixta/metabolismo , Proteínas de Plantas/metabolismo , Nódulos de las Raíces de las Plantas/enzimología , Arabidopsis/genética , Citosol/enzimología , Dioxigenasas/metabolismo , Escherichia coli/genética , Regulación de la Expresión Génica de las Plantas , Fijación del Nitrógeno/genética , Oxidorreductasas/metabolismo , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Plastidios/enzimología , Protoplastos/metabolismo , Interferencia de ARN , Nódulos de las Raíces de las Plantas/genética , Xantófilas/análisis , ZeaxantinasRESUMEN
Symbiotic nodule formation on legume roots is characterized with a series of developmental reprograming in root tissues, including extensive proliferation of cortical cells. We examined a possible involvement of the target of rapamycin (TOR) pathway, a central regulator of cell growth and proliferation in animals and yeasts, during soybean nodule development. Our results show that transcription of both GmTOR and its key downstream effector, GmS6K1, are activated during nodulation, which is paralleled with higher kinase activities of these gene products as well. RNAi-mediated knockdown of GmS6K1 impaired the nodule development with severely reduced nodule weight and numbers. In addition, expression of a few nodulins including leghemoglobin was also decreased, and consequently nitrogen fixation was found to be reduced by half. Proteomic analysis of the GmS6K1-RNAi nodules identified glutamine synthetase (GS), an essential enzyme for nitrogen assimilation in nodules, as one of the proteins that are significantly down regulated. These results appear to provide solid evidence for a functional link between GmS6K1 and nodule development.
Asunto(s)
Glycine max/enzimología , Proteínas de Plantas/genética , Interferencia de ARN , Proteínas Quinasas S6 Ribosómicas 90-kDa/genética , Nódulos de las Raíces de las Plantas/enzimología , Expresión Génica , Técnicas de Silenciamiento del Gen , Proteínas de Plantas/metabolismo , ARN Interferente Pequeño/genética , Proteínas Quinasas S6 Ribosómicas 90-kDa/metabolismo , Nódulos de las Raíces de las Plantas/genética , Nódulos de las Raíces de las Plantas/crecimiento & desarrollo , Transducción de Señal , Glycine max/genética , Glycine max/crecimiento & desarrollo , Serina-Treonina Quinasas TOR/genética , Serina-Treonina Quinasas TOR/metabolismoRESUMEN
The role of Arabidopsis S6 Kinase 1 (AtS6K1), a downstream target of TOR kinase, in controlling plant growth and ribosome biogenesis was characterized after generating transgenic plants expressing AtS6K1 under auxin-inducible promoter. Down regulation of selected cell cycle regulatory genes upon auxin treatment was observed in the transgenic plants, confirming the negative regulatory role of AtS6K1 in the plant cell cycle progression reported earlier. Callus tissues established from these transgenic plants grew to larger cell masses with more number of enlarged cells than untransformed control, demonstrating functional implication of AtS6K1 in the control of plant cell size. The observed negative correlation between the expression of AtS6K1 and the cell cycle regulatory genes, however, was completely reversed in protoplasts generated from the transgenic plants expressing AtS6K1, suggesting a possible existence of dual regulatory mechanism of the plant cell cycle regulation mediated by AtS6K1. An alternative method of kinase assay, termed "substrate-mediated kinase pull down", was employed to examine the additional phosphorylation on other domains of AtS6K1 and verified the phosphorylation of both amino- and carboxy-terminal domains, which is a novel finding regarding the phosphorylation target sites on plant S6Ks by upstream regulatory kinases. In addition, this kinase assay under the stress conditions revealed the salt- and sugar-dependencies of AtS6K1 phosphorylations.
Asunto(s)
Arabidopsis/metabolismo , Ciclo Celular/genética , Células Vegetales/metabolismo , Desarrollo de la Planta/fisiología , Proteínas de Plantas/metabolismo , Proteínas Quinasas S6 Ribosómicas/metabolismo , Arabidopsis/efectos de los fármacos , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Ciclo Celular/efectos de los fármacos , Aumento de la Célula/efectos de los fármacos , Tamaño de la Célula/efectos de los fármacos , ADN Ribosómico/genética , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Ácidos Indolacéticos/farmacología , Fosforilación , Células Vegetales/efectos de los fármacos , Células Vegetales/enzimología , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas , Protoplastos/efectos de los fármacos , Protoplastos/metabolismo , Proteínas Quinasas S6 Ribosómicas/genética , Ribosomas/efectos de los fármacos , Ribosomas/genética , Ribosomas/metabolismo , Transcripción Genética/efectos de los fármacosRESUMEN
The cell wall is a critical extracellular structure that provides protection and structural support in plant cells. To study the biological function of the cell wall and the regulation of cell wall resynthesis, we examined cellular responses to enzymatic removal of the cell wall in rice (Oryza sativa) suspension cells using proteomic approaches. We find that removal of cell wall stimulates cell wall synthesis from multiple sites in protoplasts instead of from a single site as in cytokinesis. Nucleus DAPI stain and MNase digestion further show that removal of the cell wall is concomitant with substantial chromatin reorganization. Histone post-translational modification studies using both Western blots and isotope labeling assisted quantitative mass spectrometry analyses reveal that substantial histone modification changes, particularly H3K18(AC) and H3K23(AC), are associated with the removal and regeneration of the cell wall. Label-free quantitative proteome analyses further reveal that chromatin associated proteins undergo dramatic changes upon removal of the cell wall, along with cytoskeleton, cell wall metabolism, and stress-response proteins. This study demonstrates that cell wall removal is associated with substantial chromatin change and may lead to stimulation of cell wall synthesis using a novel mechanism.
Asunto(s)
Pared Celular/metabolismo , Ensamble y Desensamble de Cromatina/fisiología , Histonas/metabolismo , Oryza/fisiología , Proteínas de Plantas/metabolismo , Acetilación , Secuencia de Aminoácidos , Western Blotting , Pared Celular/química , Microscopía Fluorescente , Datos de Secuencia Molecular , Oryza/metabolismo , Mapeo Peptídico , Extractos Vegetales/química , Procesamiento Proteico-Postraduccional , Proteómica , Protoplastos/química , Protoplastos/metabolismoRESUMEN
A leucine-rich repeat receptor-like kinase (LRR-RLK) encoded by one of the genes highly expressed in a specific stage of soybean seed development, referred to as GmLRK1, was identified and characterized. Examination of its kinase domain indicated that GmLRK1 may be a catalytically inactive atypical receptor kinase. An autophosphorylation assay confirmed that GmLRK1 is incapable of autophosphorylation in vitro. However, the phosphorylation of GmRLK1 could be induced after incubation with plant protein extracts, suggesting that some plant proteins may interact with GmLRK1 and phosphorylate the protein in vivo. Analyses of the expression profiles of GmLRK1 and its Arabidopsis ortholog At2g36570 revealed that they may be involved in regulation of more fundamental metabolic and/or developmental pathways, rather than a specialized developmental program such as seed development. Our results further indicate that the GmLRK1 and At2g36570 may play a role in the regulation of certain cellular processes that lead to cell elongation and expansion.
Asunto(s)
Aumento de la Célula , Glycine max/citología , Glycine max/genética , Proteínas Serina-Treonina Quinasas/genética , Proteínas de Soja/genética , Secuencia de Aminoácidos , Arabidopsis/citología , Arabidopsis/enzimología , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , ADN Complementario/química , ADN Complementario/genética , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Regulación Enzimológica de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Hibridación in Situ , Datos de Secuencia Molecular , Mutación , Fosforilación , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Tirosina Quinasas Receptoras/genética , Proteínas Tirosina Quinasas Receptoras/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Semillas/enzimología , Semillas/genética , Semillas/crecimiento & desarrollo , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Proteínas de Soja/metabolismo , Glycine max/enzimologíaRESUMEN
Callose synthesis occurs at specific stages of plant cell wall development in all cell types, and in response to pathogen attack, wounding and physiological stresses. We determined the expression pattern of "upstream regulatory sequence" of 12 Arabidopsis callose synthase genes (CalS1-12) genes and demonstrated that different callose synthases are expressed specifically in different tissues during plant development. That multiple CalS genes are expressed in the same cell type suggests the possibility that CalS complex may be constituted by heteromeric subunits. Five CalS genes were induced by pathogen (Hyaloperonospora arabidopsis, previously known as Peronospora parasitica, the causal agent of downy mildew) or salicylic acid (SA), while the other seven CalS genes were not affected by these treatments. Among the genes that are induced, CalS1 and CalS12 showed the highest responses. In Arabidopsis npr1 mutant, impaired in response of pathogenesis related (PR) genes to SA, the induction of CalS1 and CalS12 genes by the SA or pathogen treatments was significantly reduced. The patterns of expression of the other three CalS genes were not changed significantly in the npr1 mutant. These results suggest that the high induction observed of CalS1 and CalS12 is Npr1 dependent while the weak induction of five CalS genes is Npr1 independent. In a T-DNA knockout mutant of CalS12, callose encasement around the haustoria on the infected leaves was reduced and the mutant was found to be more resistant to downy mildew as compared to the wild type plants.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Arabidopsis/microbiología , Regulación de la Expresión Génica de las Plantas , Glucosiltransferasas/genética , Peronospora/fisiología , Transducción de Señal , Acetatos/farmacología , Arabidopsis/efectos de los fármacos , Arabidopsis/genética , Ciclopentanos/farmacología , ADN Bacteriano/genética , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Glucanos/metabolismo , Glucosiltransferasas/metabolismo , Glucuronidasa/metabolismo , Modelos Genéticos , Mutación/genética , Especificidad de Órganos/efectos de los fármacos , Especificidad de Órganos/genética , Oxilipinas/farmacología , Peronospora/efectos de los fármacos , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Plantas Modificadas Genéticamente , Ácido Salicílico/farmacología , Transducción de Señal/efectos de los fármacos , Esporas/efectos de los fármacosRESUMEN
Building a cell plate during cytokinesis in plant cells requires the participation of a number of proteins in a multistep process. We previously identified phragmoplastin as a cell plate-specific protein involved in creating a tubulovesicular network at the cell plate. We report here the identification and characterization of a phragmoplastin-interacting protein, PHIP1, in Arabidopsis (Arabidopsis thaliana). It contains multiple functional motifs, including a lysine-rich domain, two RNA recognition motifs, and three CCHC-type zinc fingers. Polypeptides with similar motif structures were found only in plant protein databases, but not in the sequenced prokaryotic, fungal, and animal genomes, suggesting that PHIP1 represents a plant-specific RNA-binding protein. In addition to phragmoplastin, two Arabidopsis small GTP-binding proteins, Rop1 and Ran2, are also found to interact with PHIP1. The zinc fingers of PHIP1 were not required for its interaction with Rop1 and phragmoplastin, but they may participate in its binding with the Ran2 mRNA. Immunofluorescence, in situ RNA hybridization, and green fluorescent protein tagging experiments showed the association of PHIP1 with the forming cell plate during cytokinesis. Taken together, our data suggest that PHIP1 is a novel RNA-binding protein and may play a unique role in the polarized mRNA transport to the vicinity of the cell plate.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Citocinesis , Proteínas de Unión al GTP/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Unión al ARN/metabolismo , Proteínas ras/metabolismo , Arabidopsis/química , Proteínas de Arabidopsis/química , Guanosina Trifosfato/metabolismo , Proteínas de la Membrana/metabolismo , Estructura Terciaria de Proteína , Proteínas de Unión al ARN/química , Dedos de ZincRESUMEN
TARGET OF RAPAMYCIN (TOR) kinase controls many cellular functions in eukaryotic cells in response to stress and nutrient availability and was shown to be essential for embryonic development in Arabidopsis thaliana. We demonstrated that Arabidopsis RAPTOR1 (a TOR regulatory protein) interacts with the HEAT repeats of TOR and that RAPTOR1 regulates the activity of S6 kinase (S6K) in response to osmotic stress. RAPTOR1 also interacts in vivo with Arabidopsis S6K1, a putative substrate for TOR. S6K1 fused to green fluorescent protein and immunoprecipitated from tobacco (Nicotiana tabacum) leaves after transient expression was active in phosphorylating the Arabidopsis ribosomal S6 protein. The catalytic domain of S6K1 could be phosphorylated by Arabidopsis 3-phosphoinositide-dependent protein kinase-1 (PDK1), indicating the involvement of PDK1 in the regulation of S6K. The S6K1 activity was sensitive to osmotic stress, while PDK1 activity was not affected. However, S6K1 sensitivity to osmotic stress was relieved by co-overexpression of RAPTOR1. Overall, these observations demonstrated the existence of a functional TOR kinase pathway in plants. However, Arabidopsis seedlings do not respond to normal physiological levels of rapamycin, which appears to be due its inability to bind to the Arabidopsis homolog of FKBP12, a protein that is essential for the binding of rapamycin with TOR. Replacement of the Arabidopsis FKBP12 with the human FKBP12 allowed rapamycin-dependent interaction with TOR. Since homozygous mutation in TOR is lethal, it suggests that this pathway is essential for integrating the stress signals into the growth regulation.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Quinasas S6 Ribosómicas/metabolismo , Transducción de Señal , Proteínas Quinasas Dependientes de 3-Fosfoinosítido , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Exones/genética , Expresión Génica , Regulación de la Expresión Génica de las Plantas , Datos de Secuencia Molecular , Presión Osmótica , Fosfatidilinositol 3-Quinasas , Fosforilación , Plantas Modificadas Genéticamente/anatomía & histología , Unión Proteica , Estructura Terciaria de Proteína , Transporte de Proteínas , Proteínas Quinasas S6 Ribosómicas/química , Proteína 1A de Unión a Tacrolimus/metabolismoRESUMEN
Nodules are formed on legume roots as a result of signaling between symbiotic partners and in response to the activities of numerous genes. We cloned fragments of differentially expressed genes in spot-inoculated soybean (Glycine max) roots. Many of the induced clones were similar to known genes related to oxidative stress, such as thioredoxin and beta-carotene hydroxylase. The deduced amino acid sequences of full-length soybean cDNAs for thioredoxin and beta-carotene hydroxylase were similar to those in other species. In situ RNA hybridization revealed that the thioredoxin gene is expressed on the pericycle of 2-d-old nodules and in the infected cells of mature nodules, suggesting that thioredoxin is involved in nodule development. The thioredoxin promoter was found to contain a sequence resembling an antioxidant responsive element. When a thioredoxin mutant of yeast was transformed with the soybean thioredoxin gene it became hydrogen peroxide tolerant. These observations prompted us to measure reactive oxygen species levels. These were decreased by 3- to 5-fold in 7-d-old and 27-d-old nodules, coincident with increases in the expression of thioredoxin and beta-carotene hydroxylase genes. Hydrogen peroxide-producing regions identified with cerium chloride were found in uninoculated roots and 2-d-old nodules, but not in 7-d-old and 27-d-old nodules. RNA interference-mediated repression of the thioredoxin gene severely impaired nodule development. These data indicate that antioxidants such as thioredoxin are essential to lower reactive oxygen species levels during nodule development.
Asunto(s)
Glycine max/crecimiento & desarrollo , Glycine max/metabolismo , Proteínas de Plantas/biosíntesis , Tiorredoxinas/biosíntesis , Secuencia de Aminoácidos , Secuencia de Bases , ADN Complementario/genética , ADN Complementario/aislamiento & purificación , ADN de Plantas/genética , ADN de Plantas/aislamiento & purificación , Genes de Plantas , Peróxido de Hidrógeno/farmacología , Oxigenasas de Función Mixta/genética , Datos de Secuencia Molecular , Proteínas de Plantas/genética , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Raíces de Plantas/microbiología , Regiones Promotoras Genéticas , Interferencia de ARN , Especies Reactivas de Oxígeno/metabolismo , Homología de Secuencia de Aminoácido , Transducción de Señal , Glycine max/genética , Simbiosis , Tiorredoxinas/genéticaRESUMEN
Callose (beta-1,3-glucan) is produced at different locations in response to biotic and abiotic cues. Arabidopsis contains 12 genes encoding callose synthase (CalS). We demonstrate that one of these genes, CalS5, encodes a callose synthase which is responsible for the synthesis of callose deposited at the primary cell wall of meiocytes, tetrads and microspores, and the expression of this gene is essential for exine formation in pollen wall. CalS5 encodes a transmembrane protein of 1923 amino acid residues with a molecular mass of 220 kDa. Knockout mutations of the CalS5 gene by T-DNA insertion resulted in a severe reduction in fertility. The reduced fertility in the cals5 mutants is attributed to the degeneration of microspores. However, megagametogenesis is not affected and the female gametes are completely fertile in cals5 mutants. The CalS5 gene is also expressed in other organs with the highest expression in meiocytes, tetrads, microspores and mature pollen. Callose deposition in the cals5 mutant was nearly completely lacking, suggesting that this gene is essential for the synthesis of callose in these tissues. As a result, the pollen exine wall was not formed properly, affecting the baculae and tectum structure and tryphine was deposited randomly as globular structures. These data suggest that callose synthesis has a vital function in building a properly sculpted exine, the integrity of which is essential for pollen viability.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Glucosiltransferasas/metabolismo , Polen/metabolismo , Arabidopsis/citología , Regulación de la Expresión Génica de las Plantas , Meiosis/fisiología , Polen/ultraestructuraRESUMEN
Living cells constantly adjust the composition and size of their membrane systems to accommodate the demands for the housekeeping activities, to expand and reduce cell size, and to commit the cell for division. Although it is well known that vesicles are the vehicles to deliver and retrieve lipids and proteins to and from the membranes, the mechanisms allowing vesicles to pinch off from membranes or fuse into a flat lipid bilayer have been poorly understood, particularly in plants. Recent studies on dynamins and dynamin-related proteins in animals and plants now allow new concepts in membrane dynamics to be considered.
Asunto(s)
Membrana Celular/fisiología , Vesículas Citoplasmáticas/fisiología , Transporte Biológico Activo/fisiología , Membrana Celular/ultraestructura , Dinaminas/fisiología , Endocitosis , ExocitosisRESUMEN
We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) domain and a proline-rich (PR) motif, characteristics of animal dynamins. All four proteins interacted in yeast two-hybrid assays with phragmoplastin, and showed different patterns of localization at the forming cell plate during cytokinesis. GFP-tagged DRP1A and DRP1C proteins were found to be associated with the cytoskeleton in G1 phase of the cell cycle. The distribution pattern of DRP1A was sensitive to propyzamid and insensitive to cytochalasin D, suggesting that DRP1A is associated with microtubules and not actin filaments. The association of DRP1A with microtubules was confirmed in vitro by spin-down assays. A GTPase-defective phragmoplastin acted as a dominant negative mutant, reduced transport of vesicles to the cell plate and formed dense tubule-like structures in the cell plate. We propose that DRP1 proteins may provide an anchor for Golgi-derived vesicles to attach to microtubules, which in turn direct the vesicles to the forming cell plate during cytokinesis. Whereas the DRP1 subfamily members are involved in tubulization of membranes, DRP2 may be involved in endocytosis and membrane recycling via clathrin-coated vesicles.
Asunto(s)
Microtúbulos/metabolismo , Proteínas de Plantas/metabolismo , Actinas/metabolismo , Secuencia de Aminoácidos , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Secuencia de Bases , División Celular/fisiología , Línea Celular , Dimerización , GTP Fosfohidrolasas/genética , GTP Fosfohidrolasas/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas Fluorescentes Verdes , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Meiosis , Microscopía Confocal , Microscopía Electrónica , Mutación , Proteínas de Plantas/química , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Unión Proteica , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Transporte de Proteínas , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Esporas/genética , Nicotiana/citología , Nicotiana/genética , Nicotiana/ultraestructura , TransfecciónRESUMEN
Pro has been shown to play an important role in ameliorating environmental stress in plants and microorganisms, including heavy metal stress. Here, we describe the effects of the expression of a mothbean delta(1)-pyrroline-5-carboxylate synthetase (P5CS) gene in the green microalga Chlamydomonas reinhardtii. We show that transgenic algae expressing the mothbean P5CS gene have 80% higher free-Pro levels than wild-type cells, grow more rapidly in toxic Cd concentrations (100 microM), and bind fourfold more Cd than wild-type cells. In addition, Cd-K edge extended x-ray absorption fine structure studies indicated that Cd does not bind to free Pro in transgenic algae with increased Pro levels but is coordinated tetrahedrally by sulfur of phytochelatin. In contrast to P5CS-expressing cells, Cd is coordinated tetrahedrally by two oxygen and two sulfur atoms in wild-type cells. Measurements of reduced/oxidized GSH ratios and analyses of levels of malondialdehyde, a product of the free radical damage of lipids, indicate that free Pro levels are correlated with the GSH redox state and malondialdehyde levels in heavy metal-treated algae. These results suggest that the free Pro likely acts as an antioxidant in Cd-stressed cells. The resulting increased GSH levels facilitate increased phytochelatin synthesis and sequestration of Cd, because GSH-heavy metal adducts are the substrates for phytochelatin synthase.
Asunto(s)
Chlamydomonas reinhardtii/genética , Metales Pesados/toxicidad , Prolina/metabolismo , 1-Pirrolina-5-Carboxilato Deshidrogenasa , Algoritmos , Aminoaciltransferasas/metabolismo , Animales , Cadmio/administración & dosificación , Cadmio/toxicidad , Chlamydomonas reinhardtii/efectos de los fármacos , Chlamydomonas reinhardtii/metabolismo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Glutatión/metabolismo , Organismos Modificados Genéticamente , Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH/genética , Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH/metabolismo , Espectrometría por Rayos XRESUMEN
Cytokinesis in plant cells is more complex than in animals, as it involves building a cell plate as the final step in generating two cells. The cell plate is built in the center of phragmoplast by fusion of Golgi-derived vesicles. This step imposes an architectural problem where ballooning of the fused structures has to be avoided to create a plate instead. This is apparently achieved by squeezing the vesicles into dumbbell-shaped vesicle-tubule-vesicle (VTV) structures with the help of phragmoplastin, a homolog of dynamin. These structures are fused at their ends in a star-shaped body creating a tubulovesicular "honeycomb-like" structure sandwiched between the positive ends of the phragmoplast microtubules. This review summarizes our current understanding of various mechanisms involved in budding-off of Golgi vesicles, delivery and fusion of vesicles to initiate cell plate, and the synthesis of polysaccharides at the forming cell plate. Little is known about the molecular mechanisms involved in determining the site, direction, and the point of attachment of the growing cell plate with the parental cell wall. These gaps may be filled soon, as many genes that have been identified by mutations are analyzed and functions of their products are deciphered.