RESUMEN
The study aimed to evaluate in vitro antioxidant, anticholinesterase and antidiabetic properties of Ouratea hexasperma (A. St.-Hil.) Baill. The inflorescence methanol extract and the ethyl acetate fraction of leaves and stems reported the highest Relative Antioxidant Capacity Index (RACI), whereas the dichloromethane fraction of leaves was the best inhibitor of α-amylase and α-glucosidase. Trans-3-O-methyl-resveratrol-2-C-ß-glucoside, lithospermoside, 2,5-dimethoxy-p-benzoquinone, lup-20(30)-ene-3ß,28-diol, 7-O-methylgenistein, apigenin and luteolin and amentoflavone were isolated from O. hexasperma. Resveratrol derivative was isolated for the first time in Ochnaceae family. Luteolin, followed by apigenin, reported the highest Relative Antioxidant Capacity Index and they were also the best inhibitors of α-glucosidase enzyme.
Asunto(s)
Antioxidantes/farmacología , Inhibidores de la Colinesterasa/farmacología , Hipoglucemiantes/farmacología , Ochnaceae/química , Antioxidantes/química , Apigenina/aislamiento & purificación , Apigenina/farmacología , Inhibidores de la Colinesterasa/química , Evaluación Preclínica de Medicamentos/métodos , Inhibidores de Glicósido Hidrolasas/farmacología , Hipoglucemiantes/química , Luteolina/aislamiento & purificación , Luteolina/farmacología , Metanol/química , Extractos Vegetales/química , Hojas de la Planta/química , alfa-Amilasas/antagonistas & inhibidoresRESUMEN
The present study deals with the evaluation of antidiabetic activities of Fagioli di Sarconi beans (Phaseolus vulgaris), including 21 ecotypes protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (southern Italy). For this purpose, α-glucosidase and α-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of Verdolino extracts exhibited the highest α-glucosidase and α-amylase inhibitory activity with IC50=1.1±0.1µg/ml and IC50=19.3±1.1µg/ml, respectively. Phytochemical compound screening of all Fagioli di Sarconi beans performed by flow injection-electrospray ionization-ultrahigh resolution mass spectrometry (uHRMS) and based on the calculation of elemental formulas from accurate m/z values, was helpful to annotate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most likely responsible for their biological activity.