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1.
Int J Hyg Environ Health ; 247: 114071, 2023 01.
Artículo en Inglés | MEDLINE | ID: mdl-36446273

RESUMEN

Per- and polyfluoroalkyl substances (PFASs) are a highly persistent, mobile, and bioaccumulative class of chemicals, of which emissions into the environment result in long-lasting contamination with high probability for causing adverse effects to human health and the environment. Within the European Biomonitoring Initiative HBM4EU, samples and data were collected in a harmonized way from human biomonitoring (HBM) studies in Europe to derive current exposure data across a geographic spread. We performed mixture risk assessments based on recent internal exposure data of PFASs in European teenagers generated in the HBM4EU Aligned Studies (dataset with N = 1957, sampling years 2014-2021). Mixture risk assessments were performed based on three hazard-based approaches: the Hazard Index (HI) approach, the sum value approach as used by the European Food Safety Authority (EFSA) and the Relative Potency Factor (RPF) approach. The HI approach resulted in the highest risk estimates, followed by the RPF approach and the sum value approach. The assessments indicate that PFAS exposure may result in a health risk in a considerable fraction of individuals in the HBM4EU teenager study sample, thereby confirming the conclusion drawn in the recent EFSA scientific opinion. This study underlines that HBM data are of added value in assessing the health risks of aggregate and cumulative exposure to PFASs, as such data are able to reflect exposure from different sources and via different routes.


Asunto(s)
Monitoreo Biológico , Fluorocarburos , Adolescente , Humanos , Medición de Riesgo , Inocuidad de los Alimentos , Bioacumulación
2.
Toxicol In Vitro ; 59: 1-11, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-30946968

RESUMEN

At a joint workshop organized by RIVM and BfR, international experts from governmental institutes, regulatory agencies, industry, academia and animal welfare organizations discussed and provided recommendations for the development, validation and implementation of innovative 3R approaches in regulatory toxicology. In particular, an evolutionary improvement of our current approach of test method validation in the context of defined approaches or integrated testing strategies was discussed together with a revolutionary approach based on a comprehensive description of the physiological responses of the human body to chemical exposure and the subsequent definition of relevant and predictive in vitro, in chemico or in silico methods. A more comprehensive evaluation of biological relevance, scientific validity and regulatory purpose of new test methods and assessment strategies together with case studies that provide practical experience with new approaches were discussed as essential steps to build up the necessary confidence to facilitate regulatory acceptance.


Asunto(s)
Toxicología/métodos , Alternativas a las Pruebas en Animales , Animales , Agencias Gubernamentales , Regulación Gubernamental , Humanos , Medición de Riesgo , Pruebas de Toxicidad/métodos , Toxicología/legislación & jurisprudencia
3.
Toxicol In Vitro ; 50: 62-74, 2018 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-29501630

RESUMEN

This report describes the proceedings of the BfR-RIVM workshop on validation of alternative methods which was held 23 and 24 March 2017 in Berlin, Germany. Stakeholders from governmental agencies, regulatory authorities, universities, industry and the OECD were invited to discuss current problems concerning the regulatory acceptance and implementation of alternative test methods and testing strategies, with the aim to develop feasible solutions. Classical validation of alternative methods usually involves one to one comparison with the gold standard animal study. This approach suffers from the reductionist nature of an alternative test as compared to the animal study as well as from the animal study being considered as the gold standard. Modern approaches combine individual alternatives into testing strategies, for which integrated and defined approaches are emerging at OECD. Furthermore, progress in mechanistic toxicology, e.g. through the adverse outcome pathway approach, and in computational systems toxicology allows integration of alternative test battery results into toxicity predictions that are more fine-tuned to the human situation. The road towards transition to a mechanistically-based human-focused hazard and risk assessment of chemicals requires an open mind towards stepping away from the animal study as the gold standard and defining human biologically based regulatory requirements for human hazard and risk assessment.


Asunto(s)
Alternativas a las Pruebas en Animales/métodos , Medición de Riesgo/métodos , Pruebas de Toxicidad/métodos , Animales , Agencias Gubernamentales , Humanos , Reproducibilidad de los Resultados
4.
Andrology ; 4(4): 662-72, 2016 07.
Artículo en Inglés | MEDLINE | ID: mdl-27152447

RESUMEN

Migration of chemicals from packaging materials to foods may lead to human exposure. Polyfluoroalkyl substances (PFAS) can be used in technical mixtures (TMs) for use in food packaging of paper and board, and PFAS have been detected in human serum and umbilical cord blood. The specific structures of the PFAS in TMs are often unknown, but polyfluorinated alkyl phosphate esters (PAPs) have been characterized in TMs, food packaging, and in food. PAPs can be metabolized into fluorotelomer alcohols (FTOHs) and perfluoroalkyl carboxylic acids (PFCAs). Some PFAS have endocrine activities, highlighting the need to investigate these effects. Herein, we studied the endocrine activity of less characterized PFAS, including short-chain PFCAs and FTOHs, PAPs, and TMs of unknown chemical composition. Long-chain PFCAs were also included. We applied seven assays covering effects on estrogen, glucocorticoid, androgen, and peroxisome proliferator-activated receptor (PPAR) activity, as well as steroidogenesis in vitro and ex vivo. In general, PAPs, FTOHs, TMs, and long-chain PFCAs showed estrogenic activity through receptor activation and/or increasing 17ß-estradiol levels. Furthermore, short- and long-chain PFCAs activated PPARα and PPARγ. Collectively, this means that (i) PAPs, FTOHs, and PFCAs exhibit endocrine activity through distinct and sometimes different mechanisms, (ii) two out of three tested TMs exhibited estrogenic activity, and (iii) short-chain FTOHs showed estrogenic activity and short-chain PFCAs generally activate both PPARα and PPARγ with similar potency and efficacy as long-chain PFCAs. In conclusion, several new and divergent toxicological targets were identified for different groups of PFAS.


Asunto(s)
Corteza Suprarrenal/efectos de los fármacos , Disruptores Endocrinos/farmacología , Hidrocarburos Fluorados/farmacología , Corteza Suprarrenal/citología , Corteza Suprarrenal/metabolismo , Línea Celular , Estradiol/metabolismo , Humanos , Progesterona/metabolismo , Testosterona/metabolismo
5.
Toxicol Sci ; 152(1): 244-56, 2016 07.
Artículo en Inglés | MEDLINE | ID: mdl-27122241

RESUMEN

Parabens comprise a group of preservatives commonly added to cosmetics, lotions, and other consumer products. Butylparaben has estrogenic and antiandrogenic properties and is known to reduce sperm counts in rats following perinatal exposure. Whether butylparaben exposure can affect other endocrine sensitive endpoints, however, remains largely unknown. In this study, time-mated Wistar rats (n = 18) were orally exposed to 0, 10, 100, or 500 mg/kg bw/d of butylparaben from gestation day 7 to pup day 22. Several endocrine-sensitive endpoints were adversely affected. In the 2 highest dose groups, the anogenital distance of newborn male and female offspring was significantly reduced, and in prepubertal females, ovary weights were reduced and mammary gland outgrowth was increased. In male offspring, sperm count was significantly reduced at all doses from 10 mg/kg bw/d. Testicular CYP19a1 (aromatase) expression was reduced in prepubertal, but not adult animals exposed to butylparaben. In adult testes, Nr5a1 expression was reduced at all doses, indicating persistent disruption of steroidogenesis. Prostate histology was altered at prepuberty and adult prostate weights were reduced in the high dose group. Thus, butylparaben exerted endocrine disrupting effects on both male and female offspring. The observed adverse developmental effect on sperm count at the lowest dose is highly relevant to risk assessment, as this is the lowest observed adverse effect level in a study on perinatal exposure to butylparaben.


Asunto(s)
Disruptores Endocrinos/toxicidad , Exposición Materna , Parabenos/toxicidad , Efectos Tardíos de la Exposición Prenatal , Animales , Animales Recién Nacidos , Aromatasa/genética , Aromatasa/metabolismo , Relación Dosis-Respuesta a Droga , Femenino , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Edad Gestacional , Masculino , Glándulas Mamarias Animales/efectos de los fármacos , Glándulas Mamarias Animales/patología , Ovario/efectos de los fármacos , Ovario/patología , Embarazo , Próstata/efectos de los fármacos , Próstata/patología , Ratas Wistar , Recuento de Espermatozoides , Factor Esteroidogénico 1/genética , Factor Esteroidogénico 1/metabolismo , Testículo/efectos de los fármacos , Testículo/metabolismo , Testículo/patología
6.
Toxicol Appl Pharmacol ; 266(1): 132-42, 2013 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-23142464

RESUMEN

Polyfluoroalkyl phosphate surfactants (PAPS) are widely used in food contact materials (FCMs) of paper and board and have recently been detected in 57% of investigated materials. Human exposure occurs as PAPS have been measured in blood; however knowledge is lacking on the toxicology of PAPS. The aim of this study was to elucidate the effects of six fluorochemicals on sex hormone synthesis and androgen receptor (AR) activation in vitro. Four PAPS and two metabolites, perfluorooctanoic acid (PFOA) and 8:2 fluorotelomer alcohol (8:2 FTOH) were tested. Hormone profiles, including eight steroid hormones, generally showed that 8:2 diPAPS, 8:2 monoPAPS and 8:2 FTOH led to decreases in androgens (testosterone, dehydroepiandrosterone, and androstenedione) in the H295R steroidogenesis assay. Decreases were observed for progesterone and 17-OH-progesterone as well. These observations indicated that a step prior to progestagen and androgen synthesis had been affected. Gene expression analysis of StAR, Bzrp, CYP11A, CYP17, CYP21 and CYP19 mRNA showed a decrease in Bzrp mRNA levels for 8:2 monoPAPS and 8:2 FTOH indicating interference with cholesterol transport to the inner mitochondria. Cortisol, estrone and 17ß-estradiol levels were in several cases increased with exposure. In accordance with these data CYP19 gene expression increased with 8:2 diPAPS, 8:2 monoPAPS and 8:2 FTOH exposures indicating that this is a contributing factor to the decreased androgen and the increased estrogen levels. Overall, these results demonstrate that fluorochemicals present in food packaging materials and their metabolites can affect steroidogenesis through decreased Bzrp and increased CYP19 gene expression leading to lower androgen and higher estrogen levels.


Asunto(s)
Fluorocarburos/metabolismo , Fluorocarburos/toxicidad , Embalaje de Alimentos , Hormonas Esteroides Gonadales/antagonistas & inhibidores , Hormonas Esteroides Gonadales/biosíntesis , Caprilatos/metabolismo , Caprilatos/toxicidad , Línea Celular Tumoral , Exposición a Riesgos Ambientales/efectos adversos , Humanos , Masculino , Progesterona/antagonistas & inhibidores , Progesterona/biosíntesis , Testosterona/antagonistas & inhibidores , Testosterona/biosíntesis
7.
Int J Androl ; 31(2): 170-7, 2008 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-18067565

RESUMEN

The endocrine-disrupting potential of four commonly used azole fungicides, propiconazole, tebuconazole, epoxiconazole and ketoconazole, were tested in two short-term in vivo studies. Initially, the antiandrogenic effects of propiconazole and tebuconazole (50, 100 and 150 mg/kg body weight/day each) were examined in the Hershberger assay. In the second study, pregnant Wistar rats were dosed with propiconazole, tebuconazole, epoxiconazole or ketoconazole (50 mg/kg/day each) from gestational day (GD) 7 to GD 21. Caesarian sections were performed on dams at GD 21. Tebuconazole and propiconazole demonstrated no antiandrogenic effects at doses between 50 and 150 mg/kg body weight/day in the Hershberger assay. In the in utero exposure toxicity study, ketoconazole, a pharmaceutical to treat human fungal infections, decreased anogenital distance and reduced testicular testosterone levels, demonstrating a demasculinizing effect on male fetuses. Tebuconazole, epoxiconazole and ketoconazole induced a high-frequency of post-implantation loss, and both ketoconazole and epoxiconazole caused a marked increase in late and very late resorptions. Overall the results show that many of the commonly used azole fungicides act as endocrine disruptors in vivo, although the profile of action in vivo varies. As ketoconazole is known to implicate numerous endocrine-disrupting effects in humans, the concern for the effects of the other tested azole fungicides in humans is growing.


Asunto(s)
Antifúngicos/toxicidad , Azoles/toxicidad , Disruptores Endocrinos/toxicidad , Animales , Estradiol/metabolismo , Femenino , Feto/efectos de los fármacos , Hormona Folículo Estimulante/sangre , Hormona Luteinizante/sangre , Masculino , Exposición Materna , Embarazo , Progesterona/sangre , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Testosterona/sangre , Tiroxina/sangre
8.
Food Addit Contam ; 20(6): 583-95, 2003 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-12881133

RESUMEN

The CALUX (chemically activated luciferase expression) bioassay based on rat hepatoma (H4IIE) cells is a sensitive assay for the detection of Ah receptor agonists like 2,3,7,8-substituted chlorinated dibenzo-p-dioxins and dibenzofurans and related PCBs. In this paper, the assay was optimized and applied for monitoring levels of dioxins in human milk samples. Combination effects of dioxin-like compounds were evaluated by testing potential mechanisms of interaction between seven of the major dioxin-like compounds in human milk using the isobole method. Results showed that the compounds acted additively, indicating that the usual assumption of additivity in the risk assessment process is valid. In general the relative potencies (REPs) of the single agents were in accordance with their TEFs assigned by the World Health Organisation, except for the mono-ortho-substituted PCB118 that had a 40-fold lower REP in CALUX. The total dioxin-like activity was determined in 16 Danish human milk samples and was in the range 20.5-55.8 pg TEQ g(-1) fat. These values were compared with TEQs obtained from GC/MS analysis (range 14.8-43.6 pg TEQ-g(-1) fat) that overall were a little lower than CALUX TEQs. The results obtained with the bioassay when testing milk extracts fractionated into dioxins/furans, non-ortho PCB and mono/di-ortho PCB fractions indicated that the correlation between the bioassay and the chemical analyses depends primarily on the Ah receptor activity observed in the mono/di-ortho PCB fraction.


Asunto(s)
Bioensayo/métodos , Dioxinas/análisis , Leche Humana/química , Animales , Interacciones Farmacológicas , Cromatografía de Gases y Espectrometría de Masas , Humanos , Neoplasias Hepáticas Experimentales/patología , Ratas , Reproducibilidad de los Resultados , Células Tumorales Cultivadas
9.
Food Addit Contam ; 19 Suppl: 13-28, 2002.
Artículo en Inglés | MEDLINE | ID: mdl-11962701

RESUMEN

Food-contact materials, including paper, have to comply with a basic set of criteria concerning safety. This means that paper for food contact should not give rise to migration of components, which can endanger human health. The objectives of this pilot study were, first, to compare paper of different qualities as food-contact materials and to perform a preliminary evaluation of their suitability, from a safety point of view, and, second, to evaluate the use of different in vitro toxicity tests for screening of paper and board. Paper produced from three different categories of recycled fibres (B-D) and a raw material produced from virgin fibres (A) were obtained from industry, and extracts were examined by chemical analyses and diverse in vitro toxicity test systems. The products tested were either based on different raw materials or different treatments were applied. Paper category B was made from 40% virgin fibres, 40% unprinted cuttings from newspapers, and 20% de-inked newspapers and magazines. Paper categories C and D were based on newspapers and magazines. However, paper D was de-inked, whereas C was not. To identify constituents of the papers with a potential to migrate into foodstuff, samples of the paper products were extracted with either 99% ethanol or water. Potential migrants in the extracts were identified and semiquantified by GC-IR-MS or GC-HRMS. In parallel to the chemical analyses, a battery of four different in vitro toxicity tests with different endpoints were applied to the same extracts. (1) a cytotoxicity test using normal human skin fibroblasts. The test was based on measurements of the reduction of resazurin to resorufin by cellular redox processes and used as a screening test for acute or general toxicity; (2) a Salmonella/microsome assay (Ames test) as a screening test for mutagenic and potentially carcinogenic compounds; (3) a recombinant yeast cell bioassay as a screening test for compounds with oestrogenic activity; (4) an aryl hydrocarbon (Ah)-receptor assay (CALUX assay) as a screening test for compounds with dioxin-like activity. In addition, the papers were testedfor microbial content and, in general, the microbiological load was quite low. The following microorganisms were counted and identified on both surface and homogenized pulp samples: the total number of aerobic bacteria, the number of aerobic and anaerobic spore formers, the number of Bacillus cereus/thuringiensis, and the number of yeast and moulds. The chemical analyses showed a significantly higher amount and different composition pattern of chemicals extracted with ethanol compared with water. Analyses of the ethanol extracts showed a distinctly smaller number and lower concentrations of chemicals in extracts prepared from sample A compared with extracts of samples B-D. The compounds identified in B-D were similar, but the amounts were lower in B compared with C and D. In accordance with the chemical analyses, the water extracts were less cytotoxic than the ethanol extracts. The extract prepared from virgin fibres was less cytotoxic than the extracts prepared from paper made from recycled fibres, and extracts prepared from C was the most cytotoxic. None of the extracts showed mutagenic activity. No conclusion about the oestrogenic activity could be made, because all extracts were cytotoxic to the test organism (yeast cells). Ethanol extracts of A and B showed a negligible positive response in the Ah-receptor assay at the highest nontoxic concentration, whereas C and D showed a more pronounced effect with C being the most potent. A comparable weak effect of water extracts of samples B-D was observed, too. However, the active compound(s) was not identified by chemical analyses.


Asunto(s)
Contaminación de Alimentos/análisis , Embalaje de Alimentos , Papel , Pruebas de Toxicidad/métodos , Bioensayo/métodos , Muerte Celular , Equipo Reutilizado , Estrógenos/análisis , Etanol , Cromatografía de Gases y Espectrometría de Masas/métodos , Humanos , Pruebas de Mutagenicidad/métodos , Proyectos Piloto , Receptores de Hidrocarburo de Aril/análisis
10.
Toxicology ; 163(1): 29-38, 2001 May 28.
Artículo en Inglés | MEDLINE | ID: mdl-11376863

RESUMEN

During the last decade, the possible effects of xenobiotics on male reproductive health have resulted in great concern. More recently, evidence of antiandrogen effect in vivo by certain chemicals has been reported. The classical Hershberger in vivo assay determining organ weight changes can be improved by measuring hormone levels as well as determining changes in gene expression of androgen-responsive genes. A real-time RT-PCR method using LightCycler technology (Roche) suitable for quantitative determination of gene expression is described. The technique combines rapid thermocycling with online fluorescence detection of PCR product formation. In this study, investigation of expression of prostate specific binding protein polypeptide C3 (PBP C3) and testosterone-repressed prostatic message 2 (TRPM-2) in the ventral prostate was performed in 60-days-old castrated Wistar rats treated daily with testosterone with or without addition of flutamide or vinclozolin for 7 days in total. We show that we can quantify the level of gene expression by use of LightCycler technology, supported by changes in reproductive organ weights as well as in hormone levels, and that analysis of gene expression levels is an even more sensitive endpoint.


Asunto(s)
Antagonistas de Andrógenos/farmacología , Próstata/efectos de los fármacos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Antagonistas de Andrógenos/toxicidad , Proteína de Unión a Andrógenos/biosíntesis , Proteína de Unión a Andrógenos/genética , Animales , Clusterina , Flutamida/farmacología , Fungicidas Industriales/farmacología , Expresión Génica/efectos de los fármacos , Glicoproteínas/biosíntesis , Glicoproteínas/genética , Hormona Luteinizante/metabolismo , Masculino , Chaperonas Moleculares/biosíntesis , Chaperonas Moleculares/genética , Tamaño de los Órganos/efectos de los fármacos , Oxazoles/farmacología , Próstata/anatomía & histología , Próstata/metabolismo , Prostateína , Ratas , Ratas Wistar , Secretoglobinas , Testosterona/farmacología , Pruebas de Toxicidad/métodos , Uteroglobina
11.
Toxicology ; 158(3): 141-53, 2001 Feb 14.
Artículo en Inglés | MEDLINE | ID: mdl-11275356

RESUMEN

Polychlorinated biphenyls (PCBs) are ubiquitous environmental persistent contaminants giving rise to potential health hazard. Some PCBs exert dioxin-like activities mediated through the aryl hydrocarbon receptor. Although reports on interaction with other nuclear receptors are sparce, some congeners are hypothesized to possess endocrine disruptive potential. Here we present evidence that the three PCBs most abundant in biological extracts, 2,2',3'4,4',5-hexachlorobiphenyl (PCB#138), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB#153), and 2,2',3,4,4',5,5'-heptachlorobiphenyl (PCB#180) have pleiotropic effects on the estrogen- and androgen-receptor. In MCF-7 cells a slightly increased cell proliferation was observed at low concentrations (1-10 nM) in cells co-treated with 0.01 nM 17beta-Estradiol, whereas the compounds inhibited cell growth significantly at 1 and 10 microM. In reporter gene (ERE-tk-CAT) analysis the three congeners exhibited a significantly estrogen receptor-ligand mediated decrease of the chloramphenicol transferase activity in both control and 10 nM 17beta-estradiol induced MCF-7 cells. In addition, PCB#138 elicited a dose-dependent antagonistic effect on androgen receptor activity in transiently co-transfected Chinese Hamster Ovary cells with an IC(50), of 6.2 microM. In summary, this study indicate that the di-ortho, multiple-chloro substituted biphenyls, PCB#138, PCB#153 and PCB#180, can compete with the binding of the natural ligand to two nuclear receptors and thus possess the ability to interfere with sexual hormone regulated processes.


Asunto(s)
Contaminantes Ambientales/farmacocinética , Contaminantes Ambientales/toxicidad , Bifenilos Policlorados/farmacocinética , Bifenilos Policlorados/toxicidad , Receptores Androgénicos/fisiología , Receptores de Estrógenos/fisiología , Antagonistas de Receptores Androgénicos , Animales , Neoplasias de la Mama/genética , Neoplasias de la Mama/patología , Células CHO , División Celular/efectos de los fármacos , Cricetinae , Cricetulus , Estradiol/farmacología , Regulación Neoplásica de la Expresión Génica , Genes Reporteros/efectos de los fármacos , Humanos , Luciferasas/análisis , Receptores Androgénicos/genética , Receptores de Estrógenos/genética , Activación Transcripcional , Transfección , Células Tumorales Cultivadas
12.
Reprod Toxicol ; 15(5): 537-43, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11780961

RESUMEN

Alkylphenol ethoxylates are widely used non-ionic surfactants. Nonylphenol ethoxylate constitutes 82% of the production of all alkylphenol ethoxylates and the breakdown product of nonylphenol ethoxylate, nonylphenol (NP) has been shown to be estrogenic in both in vitro and in vivo screening assays. To determine the potential reproductive toxicity of NP, a one-generation in utero study was conducted. Rats were dosed from gestation day 11 through 18 with NP at 3, 15, or 75 mg/kg/day or diethylstilbestrol (DES) at 30 microg/kg/day. DES was used as a positive control. Both substances were given orally by gavage. Male offspring were sacrificed at postnatal day (PND) 11, 21, or 110 and reproductive parameters were evaluated. Pup birth weight and body weight and percent motile sperm at age of 110 day were significantly reduced by DES. The absolute weight of the right epididymis was significantly reduced in the DES group. The absolute weight of the right epididymis were also significantly decreased in the animals exposed to 75 or 15 mg/kg/day NP, effects which disappeared when organ weight was related to body weight. This study showed a dose-dependent effect of nonylphenol on male reproductive development at doses of 75 and 15 mg/kg bw/day based on absolute epididymal weight.


Asunto(s)
Estrógenos no Esteroides/toxicidad , Fenoles/toxicidad , Efectos Tardíos de la Exposición Prenatal , Reproducción/efectos de los fármacos , Animales , Peso al Nacer/efectos de los fármacos , Peso Corporal/efectos de los fármacos , Química Clínica , Dietilestilbestrol/toxicidad , Relación Dosis-Respuesta a Droga , Epidídimo/efectos de los fármacos , Epidídimo/patología , Femenino , Genitales/efectos de los fármacos , Hormona Luteinizante/sangre , Masculino , Tamaño de los Órganos/efectos de los fármacos , Embarazo , Ratas , Ratas Wistar , Maduración Sexual/efectos de los fármacos , Recuento de Espermatozoides , Motilidad Espermática/efectos de los fármacos , Tensoactivos/toxicidad , Pruebas de Toxicidad
13.
Chem Res Toxicol ; 13(12): 1214-22, 2000 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-11123961

RESUMEN

The use of recycled paper for the manufacture of food contact materials is widespread, but very little is known about the presence of potential contaminants in the paper. The purpose of this study was to assess the worst-case migration of estrogenic active compounds using extracts of paper for household use. Twenty different brands of kitchen rolls, nine of which were made from recycled paper and the remainder from virgin paper, were obtained from retail shops. Paper extracts were subjected to (a) determination of the total estrogenic activity by using an in vitro estrogen screen based on yeast cells stably transfected with the human estrogen receptor alpha and (b) chemical analysis and quantification by GC/MS, GC/FTIR/MS, and GC/FID for detection of a variety of estrogenic compounds. A marked estrogenic response was observed in nine of the extracts, seven of which were made from recycled paper and two from virgin paper. The chemical analysis revealed that extracts made from recycled paper contained levels of bisphenol A ranging from 0.6 to 24 mg/kg of kitchen roll, whereas extracts from virgin paper contained no bisphenol A or only negligible amounts. In contrast, 4-tert-octylphenol, 4-nonylphenols, and di-n-butyl and diisobutyl phthalate were present to a varying degree in both recycled and virgin paper with no apparent preferable distribution between the two paper types. The estrogenic response of the two extracts made from virgin paper appeared to be due partly to the presence of the preservative propyl paraben. Diisopropylnaphthalene, which turned out to be weakly estrogenic active in vitro (EC(50) = 53 microM), was detected in minor amounts in most of the extracts with the major part, ranging from 0.3 to 4.7 mg/kg of paper, found in recycled paper. Our findings that recycled kitchen rolls contain bisphenol A and other xenoestrogens may apply to other types of recycled paper used for food packaging and emphasize the importance of identifying this and other contaminants in recycled paper in general. These data indicate that bisphenol A may be useful as a purity indicator for recycled paper.


Asunto(s)
Equipo Reutilizado , Estrógenos no Esteroides/análisis , Productos Domésticos , Papel , Compuestos de Bencidrilo , Antagonistas de Estrógenos/análisis , Antagonistas de Estrógenos/farmacología , Estrógenos no Esteroides/farmacología , Cromatografía de Gases y Espectrometría de Masas , Técnicas In Vitro , Parabenos/análisis , Fenoles/análisis , Receptores de Estrógenos/efectos de los fármacos , Receptores de Estrógenos/genética , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Transfección
14.
Toxicol In Vitro ; 14(3): 227-34, 2000 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10806373

RESUMEN

Many pesticides are able to block or activate the steroid hormone receptors and/or to affect the levels of sex hormones, thereby potentially affecting the development or expression of the male and female reproductive system or both. This emphasizes the relevance of screening pesticides for a wide range of hormone-mimicking effects. Twenty-two pesticides were tested for their ability to affect CYP19 aromatase activity in human placental microsomes using the classical [(3)H](2)O method. Prochloraz, imazalil, propioconazole, fenarimol, triadimenol, triadimefon (all fungicides), and dicofol (an acaricide) gave rise to a statistically significant inhibition of aromatase activity. The IC(50)s of prochloraz, imazalil, propioconazole fenarimol, triadimenol, and triadimefon were calculated from dose-response curves to be 0.04, 0.34, 6.5, 10, 21 and 32 microM, respectively. The IC(50) of dicofol was greater than 50 microM. The positive control 4-hydroxyandrostendione (1 microM) caused an inhibition of aromatase activity by 74%. The compounds, which did not affect the aromatase activity, were bromopropylate, chlorfenvinphos, chlorobenzilate, chlorpyrifos, diuron, heptachlor, iprodion, linuron, pentachlorphenol, procymidon, propyzamide, quintozen, tetrachlorvinphos and tetradifon. With the purpose of comparing the results for fenarimol obtained with the microsomal system with data from an intact cell system, an aromatase assay based on JEG-3 cells was established. 4-Hydroxyandrostendione (1 microM) inhibited the aromatase activity in JEG-3 cells by 94%. The IC(50) for fenarimol in this system was 2 microM, slightly lower than that observed in the microsomal system. For the first time, fenarimol has been demonstrated to inhibit aromatase activity in human tissues and, furthermore, propioconazole, triadimefon, and triadimenol were identified as weak aromatase inhibitors. In conclusion, seven out of 22 tested pesticides turned out to be weak to moderate aromatase inhibitors in vitro, indicating the relevance of elucidating the endocrine effects in vivo of these- compounds.


Asunto(s)
Inhibidores de la Aromatasa , Inhibidores Enzimáticos/toxicidad , Plaguicidas/toxicidad , Femenino , Feto/efectos de los fármacos , Humanos , Masculino , Pirimidinas/toxicidad , Reproducción/efectos de los fármacos
15.
Toxicology ; 145(2-3): 173-83, 2000 Apr 14.
Artículo en Inglés | MEDLINE | ID: mdl-10771140

RESUMEN

Nine structurally different polycyclic aromatic hydrocarbons (PAHs) were tested for their ability to either agonize or antagonize the human androgen receptor (hAR) in a sensitive reporter gene assay based on CHO cells transiently cotransfected with a hAR vector and an MMTV-LUC vector. Benz[a]anthracene (B[a]A), benzo[a]pyrene (B[a]P), fluoranthene, chrysene and 7,12-dimethylbenz[a]anthracene (DMBA) were acting as antiandrogens in vitro, resulting in IC(50) values of 3.2, 3.9, 4.6, 10.3 and 10.4 microM, respectively. Only at the highest concentration tested (10 microM), a slight inhibitory effect by pyrene, phenanthrene, and anthracene was observed. In contrast, dibenzo[a,h]anthracene (DB[a,h]A) gave rise to an agonistic effect, which was added upon the effect of the androgen receptor agonist R1881 (0.1 nM). The antiandrogenic responses by PAHs (10 microM) were found to be fully reversible, determined in the presence of increasing concentrations of R1881. No cytotoxic effects of the tested compounds were observed as determined either by metabolic reduction using AlamarBlue (up to 20 microM) or determined in cells transfected with a constitutively active hAR (up to 10 microM). The well-known ability of certain PAHs to activate the Ah receptor was assessed in H4IIE liver cancer cells, stably transfected with a luciferase reporter gene system. The positive control 2,3,7,8-tetrachlorodibenzodioxin (TCDD) caused a 13-14-fold induction of luciferase activity reaching maximum activity at 0.1 nM. DB[a,h]A, B[a]P, Chrysene, B[a]A and DMBA gave rise to a 4.5-fold induction of luciferase activity at 0.03, 0.4, 0.89, 3.06, and 9.27 microM, respectively, whereas fluoranthene, pyrene, phenanthrene and anthracene were without effect. In conclusion, no clear correlation between the antiandrogenic effects and the Ah receptor activation in vitro was seen. However, the Ah receptor agonists containing four or five aromatic rings (i.e. B [a] A, B [a] P, chrysene, DMBA) appeared to be the most potent antiandrogens (with the exception of DB [a, h] A), whereas those not able to activate the Ah receptor containing three or four aromatic rings (i.e. pyrene, phenanthrene, anthracene) displayed either very weak or no antiandrogenic effect at concentrations up to 10 microM (with the exception of fluoranthene which blocked the hAR at lower concentrations, but did not activate the Ah receptor).


Asunto(s)
Contaminantes Ambientales/toxicidad , Hidrocarburos Policíclicos Aromáticos/toxicidad , Receptores Androgénicos/efectos de los fármacos , Animales , Células CHO , Cricetinae , Relación Dosis-Respuesta a Droga , Humanos , Receptores de Hidrocarburo de Aril/efectos de los fármacos
16.
Mol Cell Endocrinol ; 152(1-2): 99-110, 1999 Jun 25.
Artículo en Inglés | MEDLINE | ID: mdl-10432228

RESUMEN

Rat Leydig cells contain a phospholipase D (PLD), which can be activated by vasopressin and phorbol ester. In order to clarify which Leydig cell organelles that express PLD activity, the subcellular localization of two differently regulated PLD activities was investigated by subcellular fractionation on a 40% (v/v) self-generating Percoll gradient. PLD activities in broken cells were estimated using radiolabeled didecanoylphosphatidylcholine as a substrate. Initial experiments revealed the presence of an oleate Mg2+ -activated PLD and a phosphatidylinositol 4,5-bisphosphate-activated PLD (PIP2-PLD) in the microsomal fraction of Leydig cells. The latter activity could be further stimulated by recombinant nonmyristoylated ADP ribosylating factor 1 (ARF1) plus GTPgammaS. The peak of oleate Mg2+ -PLD activity colocalized with the plasma membrane marker, whereas the highest specific activity of the PIP2-PLD activity was found in fractions with a slightly lower density than those containing the plasma membrane and trans-Golgi marker enzymes. In order to localize phorbol ester-stimulated PLD activity in intact Leydig cells, the cells were prelabeled with [14C]-palmitate and then stimulated for 15 min with 100 nM 4-beta-phorbol-12-myristate-13-acetate (PMA) in the presence of ethanol or butanol. The PLD product [14C]-phosphatidylethanol, expressed as the percentage of total labeled phospholipids in the fraction, was slightly increased in all Percoll fractions and showed a prominent peak in the fractions containing plasma membrane, trans-Golgi, and fractions of slightly lower density. The PMA-induced formation of [14C]-phosphatidylbutanol could be inhibited dose-dependently with brefeldin A suggesting that the activation of PLD by the phorbol ester was mediated by ARF.


Asunto(s)
Células Intersticiales del Testículo/enzimología , Fosfolipasa D/metabolismo , Factor 1 de Ribosilacion-ADP , Factores de Ribosilacion-ADP , Animales , Brefeldino A/farmacología , Carcinógenos/farmacología , Proteínas de Unión al GTP/metabolismo , Glicerofosfolípidos/metabolismo , Células Intersticiales del Testículo/ultraestructura , Masculino , Orgánulos/metabolismo , Inhibidores de la Síntesis de la Proteína/farmacología , Ratas , Proteínas Recombinantes/metabolismo , Acetato de Tetradecanoilforbol/farmacología
17.
Toxicol Appl Pharmacol ; 155(2): 150-60, 1999 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-10053169

RESUMEN

Reports on increasing incidences in developmental abnormalities of the human male reproductive tract and the recent identifications of environmental chemicals with antiandrogenic activity necessitate the screening of a larger number of compounds in order to get an overview of potential antiandrogenic chemicals present in our environment. Thus, there is a great need for an effective in vitro screening method for (anti)androgenic chemicals. We have developed a rapid, sensitive, and reproducible reporter gene assay for detection of antiandrogenic chemicals. Chinese Hamster Ovary cells were cotransfected with the human androgen receptor expression vector and the mouse mammary tumour virus (MMTV)2-luciferase vector using the new nonliposomal transfection reagent FuGene. Stimulation of the cells for 24 h with the synthetic androgen receptor agonist, R1881 (10 nM), resulted in a 30- to 60-fold induction of luciferase activity. The classical antiandrogenic compounds hydroxy-flutamide, bicalutamide, spironolactone, and cyproterone acetate together with the pesticide(metabolite)s, vinclozolin, p,p'-DDE, and procymidone all potently inhibited the response to 0.1 nM R1881. Compared to the traditional calcium phosphate transfection method, this method has the advantage of being more feasible, as the assay can be scaled down to the microtiter plate format. Furthermore, the transfection reagent is noncytotoxic, allowing its addition together with the test compounds thereby reducing the hands-on laboratory time. This assay is a powerful tool for the efficient and accurate determination and quantification of the effects of antiandrogens on reporter gene transcription. To extend the application of FuGene, the reagent was shown to be superior compared to Lipofectin for transfecting MCF7 human breast cancer cells with an estrogen response element-luciferase vector. Thus, FuGene may prove to be valuable in diverse reporter gene assays involving transient transfections for screening of potential endocrine disruptors for (anti)androgenic and (anti)estrogenic properties.


Asunto(s)
Antagonistas de Andrógenos/toxicidad , Contaminantes Ambientales/toxicidad , Antagonistas de Estrógenos/toxicidad , Genes Reporteros , Transfección/métodos , Animales , Neoplasias de la Mama , Células CHO , Cricetinae , Evaluación Preclínica de Medicamentos/métodos , Humanos , Indicadores y Reactivos , Luciferasas/genética , Luciferasas/metabolismo , Virus del Tumor Mamario del Ratón/genética , Ratones , Fosfatidiletanolaminas/genética , Fosfatidiletanolaminas/metabolismo , Receptores Androgénicos/análisis , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Células Tumorales Cultivadas
18.
Food Addit Contam ; 16(12): 533-42, 1999 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-10789375

RESUMEN

Twenty pesticides were tested for their ability to activate the oestrogen receptor in vitro using an MCF7 cell proliferation assay and a Yeast Oestrogen Screen. The fungicides fenarimol, triadimefon, and triadimenol were identified as weak oestrogen receptor agonists, which at 10 microM induces a 2.0, 2.4, and 1.9-fold increase in proliferation of human MCF7 breast cancer cells (E3 clone). The relative proliferation efficiency (RPE) was 43-69%, indicating partial agonism at the oestrogen receptor. Several pesticides did not have any effect on the proliferation response after 6 days of exposure, including: chlorpyrifos, diuron, iprodion, linuron, pentachlorphenol, prochloraz, propioconazol, propyzamine, quintozen, tetrachorvinphos and tetradifon. Some pesticides resulted in a negligible proliferation response, which was not statistically significant under the present experimental conditions. These were: bromopropylate, chlorfenvinphos, chlorobenzilate, dicofol, heptachlor, and imazalil. Fenarimol and dicofol also gave rise to a positive oestrogenic response in yeast cells transfected with the oestrogen receptor alpha, whereas the remaining compounds resulted in a negative response due either to biological inactivity or cytotoxocity to the yeast cells. The EC50 for fenarimol was estimated to be 13 microM in the yeast cells, compared with an EC50 of 3 microM in the MCF7 cells, indicating higher sensitivity of the latter assay. No in vivo data for fenarimol, triadimefon or triadimenol have previously been published that support oestrogenic activity in the intact animal. Thus, from the present results we suggest that oestrogen receptor activation may not be an important mode of action for these compounds. The need to include at least two bioassays in a screening procedure and for combining in vitro and in vivo data is emphasized.


Asunto(s)
Clorofenoles/farmacología , Plaguicidas/farmacología , Receptores de Estrógenos/efectos de los fármacos , Línea Celular/efectos de los fármacos , Clorofenoles/metabolismo , Dinamarca , Sistema Endocrino/efectos de los fármacos , Humanos , Plaguicidas/metabolismo , Receptores de Estrógenos/metabolismo , Reproducción/efectos de los fármacos
19.
J Neurochem ; 69(2): 753-61, 1997 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-9231736

RESUMEN

Glutamate-induced formation of N-acylethanolamine (NAE) and N-acylphosphatidylethanolamine (NAPE) was studied in primary cultures of mouse neocortical neurons prelabeled with [14C] ethanolamine. The formation of these two lipids was dependent on the maturity of the cell culture; i.e., no glutamate-induced formation was seen in 2-day-old cultures, whereas glutamate induced a pronounced formation in 6-day-old cultures. The calcium ionophore A23187 (2 microM) stimulated, within 2 h, formation of NAPE in 2-day-old cultures (fourfold) as well as in 6-day-old cultures (eightfold). Glutamate exerted its effect via NMDA receptors as seen by the inhibitory action of the NMDA-selective receptor antagonists D-(-)-2-amino-5-phosphonovalerate and N-(1-(2-thienyl)cyclohexyl)piperidine and the lack of effect of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)/kainate-receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). In 6-day-old cultures, exposure to NMDA (100 microM for 24 h) induced a linear increase in the formation of NAPE and NAE as well as a 40-50% neuronal death, as measured by a decrease in cellular formazan formation [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay]. The increase in NAPE and NAE could be detected earlier than the neuronal death. Neither cyclic AMP, cyclic GMP, nitric oxide, protein kinase C, nor peroxidation appears to be involved in the formation of NAPE and NAE, as assessed by the use of different pharmacological agents. Exposure to 5 mM NaN3 for 8 h resulted in a >80% decrease in the cellular MTT staining and a pronounced linear increase in the formation of NAE and NAPE (reaching 25-30% of total labeling). [14C]Anandamide was also formed in [14C]arachidonic acid-labeled neurons exposed to NaN3. No NAPE formation was detected in A23187-stimulated mouse astrocytes, rat Leydig cells and cardiomyocytes, and several other cells. These results suggest that the glutamate-induced formation of NAPE and NAE was mediated by the NMDA receptor and the formation of these lipids may be associated with neuronal death.


Asunto(s)
Corteza Cerebral/metabolismo , Etanolaminas/metabolismo , Ácido Glutámico/farmacología , Neuronas/metabolismo , Fosfatidiletanolaminas/metabolismo , 2-Amino-5-fosfonovalerato/farmacología , 6-Ciano 7-nitroquinoxalina 2,3-diona/farmacología , Animales , Ácido Araquidónico/metabolismo , Ácidos Araquidónicos/metabolismo , Calcimicina/farmacología , Muerte Celular , Células Cultivadas , Corteza Cerebral/efectos de los fármacos , Embrión de Mamíferos , Endocannabinoides , Etanolamina , Ionóforos/farmacología , Ratones , Fenciclidina/análogos & derivados , Fenciclidina/farmacología , Alcamidas Poliinsaturadas , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Receptores de N-Metil-D-Aspartato/fisiología , Factores de Tiempo
20.
Cell Signal ; 9(2): 189-96, 1997 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-9113419

RESUMEN

In this paper we demonstrate for the first time that human placenta contains a cytosolic phospholipase D (PLD) activity. This activity had a pH optimum of 7.0 and was stimulated by PIP2 and inhibited by oleate. Furthermore, cytosolic PLD was stimulated by 30 microM GTP gamma S (6-14-fold) and by the small G proteins 1 microM mArf3 (2-fold) and 0.37 nM RhoA (2-fold). This is the first report to show RhoA activation of a cytosolic PLD. The activation by mArf3 was maintained after partial purification on DEAE Sepharose of the enzyme. We have previously reported the existence of a membrane-bound PLD from human placenta, which is stimulated by PIP2, but not by oleate (Vinggaard, A. M. & Hansen, H. S. (1995) Biochim. Biophys. Acta 1258, 169-176). Here we show that oleic acid and alpha-linolenic acid both dose-dependently inhibited solubilized membrane PLD (65% inhibition at 4 mM), whereas stearic acid (4 mM) had no effect. Thus, the presence of double bonds in the fatty acid is important for the inhibitory effect. Furthermore, placental membrane PLD was activated by 30 microM GTP gamma S (4-fold) and by mArf3 (1 microM) and RhoA (0.37 nM) by a factor of 3 and 2, respectively. The solubilized membrane phospholipase D was partially purified to a basal specific activity of 25-37 nmol/min/mg. This preparation was devoid of endogenous RhoA and Arf and could not be stimulated by GTP gamma S. However, mArf3 (1 microM) still activated this partially purified membrane PLD, whereas RhoA (0.37 nM) was not able to activate this PLD fraction. In conclusion, our results suggest that the human placenta contains a PLD that is located both in the cytosol and the membranes, and that is activated by PIP2, mArf3 and RhoA but inhibited by oleate.


Asunto(s)
Citosol/enzimología , Proteínas de Unión al GTP/fisiología , Fosfolipasa D/metabolismo , Placenta/enzimología , Factores de Ribosilacion-ADP , Membrana Celular/enzimología , Activación Enzimática , Guanosina 5'-O-(3-Tiotrifosfato)/farmacología , Humanos , Concentración de Iones de Hidrógeno , Ácido Oléico/farmacología , Fosfatidilinositol 4,5-Difosfato/farmacología , Fosfolipasa D/aislamiento & purificación , Transducción de Señal/fisiología , Ácido alfa-Linolénico/farmacología , Proteína de Unión al GTP rhoA
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