[Genome-wide identification of functional noncoding RNAs,].

Non-coding RNAs (ncRNAs) are functional transcripts that do not encode proteins. They are involved in many regulation pathways. The only general characteristic shared by many (but not all) known RNAs is folding into complex shapes that are crucial to function and thus should be conserved. This fact can be used for genome-wide prediction of ncRNAs. Our approach is based on computing of local base pairing probabilities and further maximizing of probability for a segment with the use of Nussinoff algorithm. We showed that it allows to efficiently predict known ncRNA and possibly some new ncRNAs.

[The role of epsilon 2/epsilon 3/epsilon 4 polymorphism of the apolipoprotein E gene in the development of dislipoproteinemia and its influence on the efficacy of the hypolipidemic therapy].

Apolipoprotein E (apoE) isoforms have different affinity to lipoprotein (LP) receptors and lipids. In comparison with the "normal" apoE3 the apoE2 affinity to receptors is strictly decreased influencing its association with hypoholesterolemia and accumulation of LP of very-low density in the plasma. The apoE4 is characterized by the increased affinity to LP receptors and is associated with hyperholesterolemia (HCHL). In the homozygotes on allele E2 the gender, age, obesity, diabetes and some other factors have an influence on conversion of hypoholesterolemia to type Ill hyperlipidemia. The ApoE4 association with HCHL may be due to its impaired recycling in hepatocytes. The ApoE isoforms influence the hypolipidemic therapy efficacy: statins and physical training were more effective in epsilon2 allele carriers and probucol and low-fat diet had the maximal effect in epsilon4 allele carriers.