RESUMEN
New inhibitors of the Na+-I- cotransporter of the thyroid in bovine thyroid slices and in bovine plasma membrane vesicles have been investigated. They include: (1) econazole; (2) 5-(N,N-hexamethylene)amiloride (HMA); and (3) dysidenin. In both systems, the kinetics of iodide transport yielded apparent Km values of 39 and 14 microM respectively. The possible interaction of each of these inhibitors with the iodide site of the Na+-I- cotransporter was tested by performing detailed transport kinetics analysis at varying iodide concentrations and at 150 mM NaCl. Econazole induced a non-competitive inhibition while dysidenin and HMA gave a mixed type of inhibition. The Ki values for dysidenin and econazole, computed from Dixon plots, were 5 and 2 microM respectively while the Ki value for HMA could not be determined. Each inhibition was reversible, indicating the absence of covalent binding of the inhibitor to the Na+-I- cotransporter.