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1.
Int Immunopharmacol ; 133: 112103, 2024 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-38648713

RESUMEN

BACKGROUND: Sepsis is often accompanied by multiple organ dysfunction, in which the incidence of cardiac injury is about 60%, and is closely related to high mortality. Recent studies have shown that Golgi stress is involved in liver injury, kidney injury, and lung injury in sepsis. However, whether it is one of the key mechanisms of sepsis-induced cardiomyopathy (SIC) is still unclear. The aim of this study is to investigate whether Golgi stress mediates SIC and the specific mechanism. METHODS: Sepsis model of male C57BL/6J mice was established by cecal ligation and puncture. To observe the effect of Golgi stress on SIC, mice were injected with Golgi stimulant (Brefeldin A) or Golgi inhibitor (Glutathione), respectively. The 7-day survival rate of mice were recorded, and myocardial injury indicators including cardiac function, myocardial enzymes, myocardial pathological tissue score, myocardial inflammatory factors, and apoptosis were detected. The morphology of Golgi was observed by immunofluorescence, and the Golgi stress indices including GM-130, GOLPH3 and Goligin97 were detected by WB and qPCR. RESULTS: After CLP, the cardiac function of mice was impaired and the levels of myocardial enzymes were significantly increased. Golgi stress was accompanied by increased myocardial inflammation and apoptosis. Moreover, the expressions of morphological proteins GM-130 and Golgin97 were decreased, and the expression of stress protein GOLPH3 was increased. In addition, Brefeldin A increased 7-day mortality and the above indicators in mice. The use of glutathione improves all of the above indicators. CONCLUSION: Golgi stress mediates SIC, and the inhibition of Golgi stress can improve SIC by inhibiting apoptosis and inflammation.


Asunto(s)
Apoptosis , Brefeldino A , Cardiomiopatías , Aparato de Golgi , Ratones Endogámicos C57BL , Sepsis , Animales , Apoptosis/efectos de los fármacos , Masculino , Sepsis/complicaciones , Sepsis/tratamiento farmacológico , Aparato de Golgi/metabolismo , Aparato de Golgi/efectos de los fármacos , Cardiomiopatías/etiología , Cardiomiopatías/tratamiento farmacológico , Ratones , Brefeldino A/farmacología , Inflamación/tratamiento farmacológico , Modelos Animales de Enfermedad , Glutatión/metabolismo , Miocardio/patología , Miocardio/metabolismo , Proteínas de la Membrana/metabolismo , Proteínas de la Membrana/genética , Humanos
2.
Shock ; 61(3): 424-432, 2024 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-38320216

RESUMEN

ABSTRACT: Background: Sepsis-induced liver injury leads to extensive necroptosis in hepatocytes, which is the main factor of liver dysfunction. This study aims to investigate the protective effect of dexmedetomidine (DEX) on septic liver and to explore whether its molecular mechanism is related to the modulation of necroptosis. Methods: The model of septic liver injury was induced by cecal ligation and puncture (CLP) in rats. DEX and necrostatin-1(Nec-1), a specific antagonist of necroptosis, were administered 1 h before CLP. The levels of arterial blood gas, serum aspartate aminotransferase, and alanine aminotransferase were measured at 6, 12 and 24 h after CLP. The survival rate was observed 24 h after CLP. Liver pathological changes and apoptosis, the contents of IL-6 and TNF-α in liver tissue homogenates, the ROS content in liver tissue, and the expression levels of RIP1, RIP3, MLKL, and HMGB1 were detected. Results: At 6, 12, and 24 h after CLP, the levels of aspartate aminotransferase, and alanine aminotransferase levels increased, and liver enzyme levels gradually increased with the progression of sepsis. In arterial blood gas analysis, P a O 2 gradually decreased and lactic acid concentration gradually increased during these three periods. The morphological impairment of liver tissues, increased apoptosis, elevated inflammatory factors (IL-6 and TNF-α), increased ROS level, and necroptosis components (RIP1, RIP3, MLKL, and HMGB1) were all observed in sepsis rats. However, these injuries can be ameliorated by pretreatment with DEX. Meanwhile, Nec-1 pretreatment also reduced the expression of RIP1, RIP3, MLKL, HMGB1, and ROS level. Conclusion: Our study suggests that DEX alleviates septic liver injury, and the mechanism is associated with the inhibition of necroptosis.


Asunto(s)
Dexmedetomidina , Proteína HMGB1 , Sepsis , Ratas , Animales , Dexmedetomidina/farmacología , Dexmedetomidina/uso terapéutico , Factor de Necrosis Tumoral alfa/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Interleucina-6 , Necroptosis , Alanina Transaminasa , Sepsis/complicaciones , Sepsis/tratamiento farmacológico , Sepsis/metabolismo , Aspartato Aminotransferasas
3.
Int J Mol Sci ; 24(14)2023 Jul 11.
Artículo en Inglés | MEDLINE | ID: mdl-37511084

RESUMEN

Target biomarkers for H2 at both the protein and genome levels are still unclear. In this study, quantitative proteomics acquired from a mouse model were first analyzed. At the same time, functional pathway analysis helped identify functional pathways at the protein level. Then, bioinformatics on mRNA sequencing data were conducted between sepsis and normal mouse models. Differential expressional genes with the closest relationship to disease status and development were identified through module correlation analysis. Then, common biomarkers in proteomics and transcriptomics were extracted as target biomarkers. Through analyzing expression quantitative trait locus (eQTL) and genome-wide association studies (GWAS), colocalization analysis on Apoa2 and sepsis phenotype was conducted by summary-data-based Mendelian randomization (SMR). Then, two-sample and drug-target, syndrome Mendelian randomization (MR) analyses were all conducted using the Twosample R package. For protein level, protein quantitative trait loci (pQTLs) of the target biomarker were also included in MR. Animal experiments helped validate these results. As a result, Apoa2 protein or mRNA was identified as a target biomarker for H2 with a protective, causal relationship with sepsis. HDL and type 2 diabetes were proven to possess causal relationships with sepsis. The agitation and inhibition of Apoa2 were indicated to influence sepsis and related syndromes. In conclusion, we first proposed Apoa2 as a target for H2 treatment.


Asunto(s)
Apolipoproteína A-II , Diabetes Mellitus Tipo 2 , Lesión Pulmonar , Sepsis , Animales , Ratones , Biomarcadores , Predisposición Genética a la Enfermedad , Estudio de Asociación del Genoma Completo , Genómica , Hidrógeno/farmacología , Hidrógeno/uso terapéutico , Polimorfismo de Nucleótido Simple , Proteómica , Sepsis/tratamiento farmacológico , Sepsis/genética , Apolipoproteína A-II/genética , Apolipoproteína A-II/metabolismo
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