Seasonal hypoxia enhances sediment iron-bound phosphorus release in a subtropical river reservoir.

Dams worldwide commonly accelerate the eutrophication of reservoirs. While the seasonal hypoxia in deep reservoirs is widely acknowledged, there is limited research on its impact on benthic phosphorus (P) cycling and P fraction release from the reservoir sediments. Here we show that seasonal hypoxia enhances sediment P release and P fluxes at the sediment-water interface (SWI) which might alter P dynamics in deep reservoirs. We conducted a detailed measurement of sediment P fractions through the SEDEX approach, combined with a labile P gradient analysis using the diffusive gradients in thin films (DGT) technique to understand P cycling patterns in sediments during the transition period from spring (oxic) to late summer (hypoxic) conditions. The sediment P pool was predominantly composed of iron-bound phosphorus (Fe-P, 76-80 %), primarily due to the widespread occurrence of lateritic red soil (rich in Fe2O3/MnO2) in subtropical areas. More organic-P was observed in summer compared to spring. A significant increase in labile P occurred at the depth of 0-4 cm and 0-1 cm in spring and summer, respectively, where sediment P release was primarily governed by the reduction of Fe-P and the generation of S2-. A higher apparent fluxes of phosphate across the SWI were observed in summer characterized by higher temperature and lower oxygen levels. The current results suggest that seasonal hypoxia was a crucial factor affecting P cycling and diffusion in deep reservoirs. These findings present important implications for the ecology and management of the watershed-coast ecosystem.

Storm-induced nitrogen transport via surface runoff, interflow and groundwater in a pomelo agricultural watershed, southeast China.

The storm-induced export of nitrogen (N) from agricultural watersheds significantly impacts aquatic ecosystems, yet the mechanisms of source supply and transport behind N species remain unclear. Here, we investigated the hydrological factors influencing the timing and magnitude of river N species export in a Chinese pomelo agricultural watershed. We conducted continuous observations of watershed hydrology, N species, and their isotopic ratios along a soil-groundwater-river continuum during two storm events in 2018-2019. We found the export flux of river NO3-N covers ∼80% of the total N flux during storms, and the rest for other N species. Our results further revealed distinct pathways and timing of N transport among different N species, especially between ammonium N (NH4-N) and nitrate N (NO3-N). NH4-N in stormflow predominantly originates from sewage and soil leachate, rapidly transported via surface runoff and interflow. Orchard fertilization (contributed 41-56% based on SIAR analysis) was the major source of river NO3-N, which underwent initial dilution via surface runoff and subsequently became enriched through delayed discharge of soil leachate and groundwater. The variations in timing and magnitude of N transport between storms can be explained by antecedent conditions such as precipitation, soil N pools, and storm size. These findings emphasize the hydrological controls on N export from agricultural watersheds, and highlight the variations in source supply and transport pathways among different N species. The insights gained from this study hold significance for managing agricultural pollution and restoring impaired aquatic systems.

Relay Catalysis for Highly Selective Conversion of Methanol to Ethylene in Syngas.

The precise C-C coupling is a challenging goal in C1 chemistry. The conversion of methanol, a cheap and easily available C1 feedstock, into value-added and largely demanded olefins has been playing a game-changing role in the production of olefins. The current methanol-to-olefin (MTO) process, however, suffers from limited selectivity to a specific olefin. Here, we present a relay-catalysis route for the high-selective conversion of methanol to ethylene in syngas (H2/CO) typically used for methanol synthesis. A bifunctional catalyst composed of selectively dealuminated H-MOR zeolite and ZnO-TiO2, which implemented methanol carbonylation with CO to acetic acid and selective acetic acid hydrogenation to ethylene in tandem, offered ethylene selectivity of 85% at complete methanol conversion at 583 K. The selective removal of Brønsted acid sites in the 12-membered ring channel of H-MOR favors the selectivity of acetic acid in CH3OH carbonylation. The high capabilities of ZnO-TiO2 in the adsorption of acetic acid and the activation of H2 play key roles in selective hydrogenation of acetic acid to ethylene. Our work provides a promising relay-catalysis strategy for precise C-C coupling of C1 to C2 molecules.

Pollution abatement reducing the river N2O emissions although it is partially offset by a warming climate: Insights from an urbanized watershed study.

Global nitrogen (N) pollution has resulted in increased river nitrous oxide (N2O) emissions, which contribute to climate change. However, little is known about how pollution abatement conversely reduces river N2O production in a warming climate. Here, field observations and microcosmic experiments were conducted in a coastal urbanized watershed (S.E. China) to explore the interactive effect of changing nitrate and temperature on river sediment denitrification (DNF) and N2O production. The results showed that urban river reaches (UR) with higher organic carbon content and denitrifying gene abundance in sediments have a greater DNF rate, nitrate removal efficiency (NRE), and N2O concentration than agricultural river reaches (AR). Microcosmic incubation suggested that the DNF rate and associated N2O production decreased under low nitrate addition, wherein the NRE increased. The scenario simulation illustrated a nonlinear response of N2O production to nitrate removal (i.e., ΔN2O/ΔNO3-N) from both UR and AR sediments at a given temperature, and the DNF rate and N2O production increased with increasing temperature. An increase in temperature by 1 degree Celsius would offset 18.75% of the N2O reduction by nitrate removal via DNF. These findings implied that watershed pollution abatement undoubtedly contributes to the reduction in global river N2O emissions although it is partially offset by extra N2O production caused by global warming.

Hydrological connectivity affects nitrogen migration and retention in the land‒river continuum.

Land use change and excessive nitrogen (N) loading threaten the health of receiving water bodies worldwide. However, the role of hydrological connectivity in linking watershed land use, N biogeochemistry and river water quality remain unclear. In this study, we investigated 15 subwatersheds in the Jiulong River watershed (southeastern China) during a dry baseflow period in 2018, combined with 3‒year (2017-2019) nutrient monitoring in 5 subwatersheds to explore river N dynamics (dissolved nutrients, dissolved gases and functional genes) and their controlling factors at three hydrological connectivity scales, i.e., watershed, hydrologically sensitive areas (HSAs) and riparian zone. The results show that land use at HSAs (less than 20% of watershed area) and watershed scales contributed similarly to river N variation, indicating that HSAs are hotspots for transporting land N into river channels. In particular, the agricultural land was the main factor affecting river nitrate and nitrous oxide (N2O) concentrations, while the built-up land significantly affected river ammonium and nitrite. At the riparian zone scale, soils and sediments substantially influenced river N retention processes (i.e., nitrification and denitrification). Management and protection measures targeting HSAs and riparian zones are expected to efficiently reduce river N loading and improve water quality.

Impacts of a subtropical storm on nitrogen functional microbes and associated cycling processes in a river-estuary continuum.

Storms, in subtropical regions such as S.E. China, cause major changes in the physical and biogeochemical fluxes of anthropogenic N species through the river-estuary continuum to the coast. Two weeks continuous observations at a sampling station (Station E) in the upper Jiulong River Estuary (S.E. China) were conducted to track the changes of physical and biogeochemical parameters together with genomic identification of nitrogen cycling microbes through a complete storm event in June 2019. In conjunction with previous N flux measurements, it was found that there was greatly increased flux of N to and through the upper estuary during the storm. During the storm, the freshwater/brackish water boundary moved downstream, and previously deposited organic rich sediment was resuspended. During baseflow, anthropogenically derived ammonium was oxidised dominantly by the marine nitrifying (AOA) microbe Nitrosopelagicus. However, during the storm, the dominant ammonia-oxidizing archaea (AOA) at Station E changed to the riverine genus (Nitrosotenuis) while the marine genus, Nitrosopumilus decreased. At the same time the dominant ammonia-oxidizing bacteria (AOB) was still the marine genus (Nitrosomanas). Estuarine nitrifiers had higher abundance, weighted entropy and diversity during the Flood, suggesting that the high NH4-N and DO during the Rising period of the Flood resulted in a bloom of nitrifiers. The changing gene abundances of nitrifiers were reflected in changes in the concentration and isotopic composition of DIN confirming active nitrification in the oxygen-rich water column. During the storm the numbers of denitrifiers (narG, nirS and nod), DNRA (nrfA) and anammox (hzsB) were found in the water column increased, and the larger fraction was associated with the <22 µm free-living fraction. However it was not possible with the data obtained to estimate what fraction of these anaerobic bacteria were active in the dominantly oxic water column.

Prevalence of group B streptococcus colonization in pregnant women in Jiangsu, East China.

BACKGROUND: Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. However, GBS was infrequently reported in the developing world in contrast to western countries. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China, and revealed the difference of GBS infection between culture and PCR. METHODS: A total of 16,184 pregnant women at 34 to 37 weeks' gestation aged 16-47 years were recruited from Nanjing Kingmed Center for Clinical Laboratory. Nine thousand twenty-two pregnant women received GBS screening by PCR detection only. Seven thousand one hundred sixty-two pregnant women received GBS screening by bacterial culture and GBS-positive samples were tested for antibiotic resistance. RESULTS: The overall GBS positive rate was 8.7% by PCR and 3.5% by culture. Colonization rate was highest in the "25-29 years" age group. The 249 GBS-positive samples which detected by culture were all sensitive to penicillin. The prevalence of resistance to erythromycin, clindamycin, and levofloxacin was 77.5, 68.3, and 52.2%, respectively. CONCLUSIONS: This study revealed the data on the prevalence of GBS colonization in pregnant women at 34 to 37 weeks' gestation in Jiangsu, East China. It compared the difference of the sensitivity to detect GBS between PCR and culture. PCR was expected to become a quick method in pregnancy women conventional detection of GBS infection.

Stretchable and Superwettable Colorimetric Sensing Patch for Epidermal Collection and Analysis of Sweat.

Stretchable and wearable sensors allow intimate integration with the human body for health and fitness monitoring. In addition to the acquisition of various physical parameters, quantitative analysis of chemical biomarkers present in sweat may provide vital insights into the physiological state of an individual. A widely investigated system utilizes electrochemical techniques for continuous monitoring of these biomarkers. The required supporting electronics and batteries are often challenging to form a deformable system. In this study, an intrinsically stretchable sensing patch is developed with compliant mechanical properties for conformal attachment to the skin and reliable collection of sweat. In these patches, superhydrophilic colorimetric assays consisting of thermoplastic polyurethane nanofiber textiles decorated with silica nanoparticles are assembled over a styrene-ethylene-butylene-styrene-based superhydrophobic substrate, thereby generating a large wettability contrast to efficiently concentrate the sweat. The system supports multiplexed colorimetric analysis of sweat to quantify pH and ion concentrations with images acquired using smartphones, in which the influence of ambient lighting conditions is largely compensated with a set of reference color markers. Successful demonstrations of insitu analysis of sweat after physical exercises effectively illustrate the practical suitability of the sensing patch, which is attractive for advanced health monitoring, clinical diagnostics, and competitive sports.

Tidal driven nutrient exchange between mangroves and estuary reveals a dynamic source-sink pattern.

Nitrogen (N) and phosphorus (P) are vital nutrients regulating mangrove productivity and coastal ecosystems. Understanding of the nutrient cycling and interaction between mangroves and estuary is limited. Here we show tidal-driven nutrient exchange and a dynamic source-sink pattern across the mangrove-estuary interface. Lateral nutrient fluxes were quantified based on hourly concentrations observed at a tidal creek outlet during 2016-2018 and water mass estimated by a hydrodynamic model (FVCOM). The results of nutrient fluxes suggested that mangroves always serve as a source of ammonium (NH4-N) and dissolved reactive P (DRP) to estuary, but as a strong nitrate sink (NO3-N). Dissolved organic components (DON and DOP) shifted from net efflux (source) in spring to net influx (sink) in summer, likely due to the changing balance of P input and biological and physicochemical processes. Mangroves decreased the overall loading of dissolved inorganic N (DIN), dissolved total N (DTN) and total P (TP) to the estuary. Nevertheless, the effluents (aquaculture wastewater and domestic sewage) discharged from the upstream area during ebb tide increased the export of nutrients, especially NH4-N and DRP, offsetting the role of mangrove on mitigating coastal eutrophication.

mRNA Expression of FGFR1 as Potential Marker for Predicting Prognosis of Surgical Resection of Small Cell Lung Cancer may be better than Protein Expression and Gene Amplification.

Purpose: Fibroblast growth factor receptor 1 (FGFR1) alterations have been described in many cancers, including lung cancer, but the role has not been elucidated specifically in small cell lung cancer (SCLC). The present study aimed to identify the frequency of FGFR1 alterations among Chinese patients with surgically resected SCLC and the association with the clinicopathological characteristics and the survival were also investigated. Methods: FGFR1 protein expression, FGFR1 amplification, FGFR1 mutations, and messenger RNA (mRNA) levels, were determined by immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR), respectively in primary tumors from 33 patients with resected SCLC. Results: 7/33(21.2%) of the specimens were positive for FGFR1 protein expression. FGFR1 amplification was identified in 4/28 cases (14.3%). If the cut-off value was determined to be 3.5, FGFR1 mRNA positivity was considered in 7/33 cases (21.2%). However, no mutation was detected in the 33 SCLC postoperative tissue specimens. No significant association was observed between FGFR1 protein expression or amplification and clinicalcharacteristics or prognosis. There was a distinct trend for mRNA level and poor prognosis, including recurrence-free survival (RFS) (p = 0.07) and overall survival (OS) (p= 0.08), but they did not reach statistical significance. Conclusions: As novel FGFR1-targeted therapies are developed, FISH, IHC, especially mRNA were detected, which should be considered as biomarkers of FGFR1 pathway dysregulation in SCLC.

EGFR exon 20 insertion mutations in non-small cell lung cancer.

Mutations in the epidermal growth factor receptor (EGFR) gene are the most common targetable genomic drivers of non-small cell lung cancer (NSCLC). 90% of the EGFR mutations comprise of EGFR exon 19 deletion and exon 21 L858R mutation, while EGFR exon 20 insertion (EGFR Ex20Ins) is the third most common type of EGFR mutation. Currently, studies on EGFR Ex20Ins are relatively scarce and limited. The frequency of EGFR Ex20Ins mutations in NSCLC was 1-10%. Patients harboring EGFR Ex20Ins exhibited similar clinical characteristics except for poorer prognosis as compared to patients with sensitizing mutations in EGFR. Conventional TKIs have poor efficacy in a majority of EGFR Ex20Ins subtypes. Chemotherapy remains the preferred treatment for advanced NSCLC patients harboring EGFR Ex20Ins. However, some novel inhibitors are considered as putative candidates. This review focuses on the structural and biochemical features, clinical characteristics, treatments, and prognosis of EGFR Ex20Ins in NSCLC.