Predictive Modeling Analysis for the Quality Indicators of Matsutake Mushrooms in Different Transport Environments.

Matsutake mushrooms, known for their high value, present challenges due to their seasonal availability, difficulties in harvesting, and short shelf life, making it crucial to extend their post-harvest preservation period. In this study, we developed three quality predictive models of Matsutake mushrooms using three different methods. The quality changes of Matsutake mushrooms were experimentally analyzed under two cases (case A: Temperature control and sealing measures; case B: Alteration of gas composition) with various parameters including the hardness, color, odor, pH, soluble solids content (SSC), and moisture content (MC) collected as indicators of quality changes throughout the storage period. Prediction models for Matsutake mushroom quality were developed using three different methods based on the collected data: multiple linear regression (MLR), support vector regression (SVR), and an artificial neural network (ANN). The comparative results reveal that the ANN outperforms MLR and SVR as the optimal model for predicting Matsutake mushroom quality indicators. To further enhance the ANN model's performance, optimization techniques such as the Levenberg-Marquardt, Bayesian regularization, and scaled conjugate gradient backpropagation algorithm techniques were employed. The optimized ANN model achieved impressive results, with an R-Square value of 0.988 and an MSE of 0.099 under case A, and an R-Square of 0.981 and an MSE of 0.164 under case B. These findings provide valuable insights for the development of new preservation methods, contributing to the assurance of a high-quality supply of Matsutake mushrooms in the market.

Modeling Impacts of Urbanization on Winter Boundary Layer Meteorology and Aerosol Pollution in the Central Liaoning City Cluster, China.

The influence of urbanization on the frequent winter aerosol pollution events in Northeast China is not fully understood. The Weather Research and Forecasting Model with Chemistry (WRF-Chem) coupled with urban canopy (UC) models was used to simulate the impact of urbanization on an aerosol pollution process in the Central Liaoning city cluster (CLCC), China. To investigate the main mechanisms of urban expansion and UC on the winter atmospheric environment and the atmospheric diffusion capacity (ADC) in the CLCC, three simulation cases were designed using land-use datasets from different periods and different UC schemes. A comparative analysis of the simulation results showed that the land-use change (LU) and both LU and UC (LUUC) effects lead to higher surface temperature and lower relative humidity and wind speed in the CLCC by decreasing surface albedo, increasing sensible heat flux, and increasing surface roughness, with a spatial distribution similar to the distribution of LU. The thermal effect leads to an increase in atmospheric instability, an increase in boundary layer height and diffusion coefficient, and an increase in the ADC. The LU and LUUC effects lead to a significant decrease in near-surface PM2.5 concentrations in the CLCC due to changes in meteorological conditions and ADC within the boundary layer. The reduction in surface PM2.5 concentrations due to the LU effect is stronger at night than during daytime, while the LUUC effect leads to a greater reduction in surface PM2.5 concentrations during the day, mainly due to stronger diffusion and dilution caused by the effect of urban turbulence within different levels caused by the more complex UC scheme. In this study, the LU and LUUC effects result in greater thermal than dynamic effects, and both have a negative impact on surface PM2.5 concentrations, but redistribute pollutants from the lower urban troposphere to higher altitudes.

Formation and evolution of secondary particulate matter during heavy haze pollution episodes in winter in a severe cold climate region of Northeast China.

The formation and evolution of sulfate (SO42-) and nitrate (NO3-) secondary contaminants under different stages of pollution episodes and different meteorological and emission conditions were compared, based on the simultaneous observation of fine particulate matter (PM2.5) and its chemical components in four heavy haze pollution episodes at 14 sampling sites in a severe cold climate region of Northeast China in winter from 2017 to 2019. The results yielded two main findings. (1) Nitrate formation during the day was mainly due to the combination of high emissions and high relative humidity (RH, 50-90%), high temperature (T, 0 to 5 °C), high atmospheric oxidizability (ozone (O3) and nitrous acid (HONO) concentrations), and high ammonia (NH3) concentrations. Nitrate was formed by a gas-phase homogeneous reaction of the hydroxyl radical (OH·) with nitrogen dioxide (NO2), sulfur dioxide (SO2), and ammonia (NH3). (2) The main differences in SO42- formation between Northeast China and other regions were that the gas-phase oxidation process played an important role. This was mainly a result of the promotion of the gas-phase oxidation of SO42- due to the high oxidizing ability and the suppression of the aqueous reaction due to the low Ts in winter and low-sulfur coal emissions. Sulfate formation mostly occurred through an aqueous phase reaction in winter, but the highest yield and the fastest production capacity were produced by the gas-phase reaction.

Multi-Year Variation of Ozone and Particulate Matter in Northeast China Based on the Tracking Air Pollution in China (TAP) Data.

With the rapid development of economy and urbanization acceleration, ozone (O3) pollution has become the main factor of urban air pollution in China after particulate matter. In this study, 90th percentile of maximum daily average (MDA) 8 h O3 (O3-8h-90per) and PM2.5 data from the Tracking Air Pollution in China (TAP) dataset were used to determine the mean annual, seasonal, monthly, and interannual distribution of O3-8h-90per and PM2.5 concentrations in Northeast China (NEC). The O3-8h-90per concentration was highest in Liaoning (>100 µg/m3), whereas the highest PM2.5 concentration was observed mainly in urban areas of central Liaoning and the Harbin−Changchun urban agglomeration (approximately 60 µg/m3). The O3-8h-90per concentrations were highest in spring and summer due to more intense solar radiation. On the contrary, the PM2.5 concentration increased considerably in winter influenced by anthropogenic activities. In May and June, the highest monthly mean O3-8h-90per concentrations were observed in central and western Liaoning, about 170−180 µg/m3, while the PM2.5 concentrations were the highest in January, February, and December, approximately 100 µg/m3. The annual mean O3-8h-90per concentration in NEC showed an increasing trend, while the PM2.5 concentration exhibited an annual decline. By 2020, the annual mean O3-8h-90per concentration in southern Liaoning had increased considerably, reaching 120−130 µg/m3. From the perspective of city levels, PM2.5 and O3-8h-90per also showed an opposite variation trend in the 35 cities of NEC. The reduced tropospheric NO2 column is consistent with the decreasing trend of the interannual PM2.5, while the increased surface temperature could be the main meteorological factor affecting the O3-8h-90per concentration in NEC. The results of this study enable a comprehensive understanding of the regional and climatological O3-8h-90per and PM2.5 distribution at distinct spatial and temporal scales in NEC.

Multilevel air quality evolution in Shenyang: Impact of elevated point emission reduction.

Visibility observed at different altitudes is favorable to understand the causes of air pollution. We conducted 4-years of observations of visibility at 2.8 and 60 m and particulate matter (PM) concentrations from 2015 to 2018 in Shenyang, a provincial city in Northeast China. The results indicated that visibility increased with the increasing height in winter (especially at night), and decreased with height in summer (especially at the daytime). PM concentration exhibited opposite vertical variation to visibility, reflecting that visibility degrades with the increase of aerosol concentration in the air. The radiosonde meteorological data showed that weak turbulence in the planetary boundary layer (PBL) in winter favored aerosols' accumulation near the surface. Whereas in summer, unstable atmospheric conditions, upper-level moister environment, and regional transport of air pollutants resulted in the deterioration of upper-level visibility. Inter-annual variation in the two-level visibility indicated that the upper-level visibility improved more significantly than low-level visibility, much likely due to the reduction in emission of elevated point sources in Shenyang. Our study suggested that strengthening the control of surface non-point emissions is a promising control strategy to improve Shenyang air quality.

Climatology and trends of aerosol optical depth with different particle size and shape in northeast China from 2001 to 2018.

Aerosol generated from the economic development and extensive urbanization in northeast China (NEC) could influence aerosol optical properties and affect the regional air quality. The level 3 aerosol optical depth (AOD) of different particle size and shape (spherical or nonspherical) obtained by Multiangle Imaging Spectroradiometer (MISR) version 23 were used to estimate their seasonal, annual, and decadal distribution and contribution in NEC from 2001 to 2018. The highest AOD of approximately 0.3 was found in the central Liaoning urban agglomeration, and the lowest AOD occurred in the mountainous area of NEC; the proportion of spherical AOD in NEC region was more than 90%. The contribution of large AOD was higher in spring, ranging from 28.8% to 29.8%. In spring and summer, small and medium AODs were concentrated in central Liaoning (approximately 0.2-0.3 and 0.06-0.08, respectively). The annual variation in the AOD of different particle size was significantly higher in Liaoning than in Jilin and Heilongjiang. The annual proportions of small and spherical AODs were approximately 60% and 90%, respectively. The annual occurrence of clean conditions with AOD < 0.05 was most common in northern Heilongjiang (approximately 20%). In NEC, the annual occurrence frequencies of 0.05 < AOD < 0.15 and AOD > 0.6 were the highest (approximately 50%) and the lowest (less than 1%), respectively. Interdecadal AOD revealed a positive trend from 2001 to 2008 and a negative trend from 2009 to 2018. The frequency of occurrence trend at different AOD levels also changed from positive to negative between these two periods. The findings in this study are based on the first aerosol retrieval of the newly released MISR in NEC. The results provide a comprehensive understanding of the regional and climatological aerosol extinction with different AOD of size and shape as well as various level bins in NEC.

Chronic toxicity study in Sprague-Dawley rats on transgenic rice T1c-19 with cry1C* gene.

In the present study, a novel transgenic rice line T1c-19 carrying cry1C* gene was evaluated in Sprague-Dawley (SD) rats by a 52-week feeding study, aiming at determining its unintended effects. The rice T1c-19 and its parental rice were prepared at a level of up to 60.75% in the growth diet and 66.75% in the maintenance diet, respectively. AIN-93 diet was used as a nutritional control. All the diets were nutritionally balanced. Each group, with 48 rats of both genders, was fed the corresponding diet for 52 weeks. The results of clinical signs, body weight and food consumption of the transgenic rice group were comparable to those of the parental rice group. Clinical measurements were made on weeks 13, 26 and 52, and statistical significances were observed in several hematological and serum biochemical indices between the two rice groups and were not considered as treatment-related. The terminal histopathological examination showed some spontaneous lesions in all groups with no significant difference among them. Taken together, the results of the present 52-week chronic toxicity study of transgenic rice T1c-19 exerted no unintended adverse effects on SD rats.

Acute and Subchronic Oral Toxicity Study of Gardenia Yellow E500 in Sprague-Dawley Rats.

OBJECTIVE: This study was conducted to evaluate the acute and subchronic toxicity of gardenia yellow, a natural colorant widely used in China and other Asian countries. An acute toxicity test was performed in S-D rats of both genders and the lethal dose (LD50) of per oral gardenia yellow was estimated to be more than 15.0 g/kg·bw. In the subchronic study, gardenia yellow was orally administered to rats by gavage at doses of 0, 0.50, 1.50 and 4.50 g/kg·bw/day for 90 days followed by a recovery period of 28 days. No appreciable toxic-related changes were observed in the 0.50 g/kg·bw/day group. When the animals received gardenia yellow at 1.50 g/kg·bw/day or more, body weight loss was observed, and pigments began to deposit in several vital organs, resulting in significant changes of several hematological and biochemical indicators related to the nutritional status of the body, liver and kidney function, more severe in the high dose group. In the recovery period, the alterations of the clinical symptoms and parameters were relieved a lot. Based on the results of the current study, the no observed adverse effect level (NOAEL) of gardenia yellow E500 in rats was set to be 0.50 g/kg·bw/day.

MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4.

PURPOSE: miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. METHODS: Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dual-luciferase reporter assay was used to analyze the direct binding between miR-497-5p and 3'-untranslated region of CBX4. Cell viability was measured with MTT assay. Flow cytometry was performed to detect cell cycle distribution. RESULTS: Here, using bioinformatics methods we firstly found that miR-497-5p regulated cervical carcinoma proliferation by targeting polycomb chromobox4 (CBX4). Expression of miR-497-5p in cervical carcinoma tissues was negatively correlated with CBX4. A binding region of miR-497-5p in 3'-untranslated region of CBX4 was predicted. Further experiments confirmed that miR-497-5p directly targeted CBX4. Besides, RNA interference of CBX4 inhibited cervical cancer cell proliferation, arrested cells at S phase and reduced the expression of CDK2 and Cyclin A2 proteins. The use of miR-497-5p inhibitor compromised CBX4 interference RNAs induced cycle arrest of cervical cancer cells. Cells co-transfected with miR-497-5p inhibitors and CBX4 interference RNAs had a higher proliferation rate than CBX4 inference RNA-transfected cells. CONCLUSION: All together, the present study demonstrates that miR-497-5p inhibits cervical cancer cells proliferation by directly targeting CBX4.

Greenhouse gas mitigation potential of balanced fertilization cropland under double-cropping systems: a case study in Shaanxi province, China.

Reducing agricultural greenhouse gas (GHG) emissions is attracting increasing attention. Balanced fertilization (BF) of cropland has been widely promoted and applied and has great potential to reduce GHG emissions. This study assesses GHG mitigation of BF cropland systems including winter wheat and summer maize double-cropping system (wheat-maize) and winter oilseed rape (Brassica napus) and rice double-cropping system (rape-rice) in Shaanxi province, China. We determined the boundaries, scenarios, leakage, and sources of GHG mitigation and developed a measurement system for GHG mitigation under these cropping systems for BF farmland. In the measurement system, except for the changes in nitrogen fertilizer rates, soil carbon storage, mechanical fuel consumption, and fertilizer management mode (paddy), change in crop yield was recommended as a primary source of GHG mitigation. The BF cropland areas of wheat-maize and rape-rice were 2818.89 ha and 1671.73 ha, respectively. The use of BF reduced the GHG emissions of wheat-maize by 1.15 tCO2 equivalent (CO2e) ha-1 per year and the emissions of rape-rice by 1.05 tCO2e ha-1 per year. The BF cropland produced 5007.6 tCO2e per year. Our results do not only provide a reference for the assessment of GHG mitigation on BF cropland under double-cropping systems, but also will be helpful for improving the methodology of GHG mitigation on BF cropland.

ZAP-70 in chronic lymphocytic leukemia: A meta-analysis.

BACKGROUND: Recent studies have reported that zeta-chain-associated protein kinase 70 (ZAP-70) expression plays a prognostic role in chronic lymphocytic leukemia (CLL). However, these results remain controversial. Thus, we performed a meta-analysis to clarify the prognostic value of ZAP-70 expression in CLL. MATERIALS AND METHODS: Relevant studies were searched in PubMed, Embase, Cochrane library, and Web of Science up to January 2018. Clinicopathological features and prognostic data were extracted from the studies. We pooled estimates and 95% confidence intervals (CIs) and estimated the heterogeneity of studies using Mantel-Haenszel or DerSimonian and Laird method. RESULTS: Twelve studies that included 1956 patients with CLL were eligible for inclusion. The pooled results revealed that increased ZAP-70 expression was significantly associated with poor overall survival (hazard ratio [HR] = 2.48, 95% CI: 1.72-3.59, P = 0.019, I2 = 53.0%) and event-free survival (HR = 4.17, 95% CI: 2.17-8.01, P = 0.014, I2 = 68.2%) in a random-effects model with significant heterogeneity. Clinicopathological analysis demonstrated that ZAP-70 expression was significantly associated with unmutated immunoglobulin heavy-chain genes, CD38 expression, serum ß-2 microglobulin, and lymphocyte doubling time. CONCLUSIONS: Our findings indicated that ZAP-70 was a strong prognostic biomarker for patients with CLL.

Gestational age and childhood leukemia: A meta-analysis of epidemiologic studies.

OBJECTIVE: An increasing amount of evidence shows that childhood leukemia is initiated in utero. Birth characteristics initiated in utero, such as gestational age, may play a role in leukemogenesis. The purpose of our meta-analysis is to explore the association between gestational age and childhood leukemia. METHODS: Relevant studies up to 21 April 2017 were collected by searching PubMed and EMBASE databases. Subgroup analysis, sensitivity analysis and publication bias assessment were conducted. RESULTS: A total of 13 studies were included. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) for preterm birth and postterm birth were 1.06 (0.98, 1.13) and 1.01 (0.90, 1.13) for childhood leukemia, 1.04 (0.97, 1.11) and 1.03 (0.95, 1.12) for acute lymphocytic leukemia (ALL), 1.20 (1.00, 1.44) and 1.20 (1.00, 1.43) for acute myeloid leukemia (AML), compared with full-term birth. Study type and study region were the reasons behind the heterogeneity. In subgroup analyses, the summary ORs with 95% CI for childhood leukemia and ALL were 1.23 (1.07, 1.41) and 1.21 (1.06, 1.39) for postterm birth in cohort studies. No significant changes in sensitivity analyses and no publication bias were observed in our analysis. CONCLUSION: Our results suggest that both preterm and postterm infants have an elevated risk of developing AML. In addition, postterm birth increased the risk of childhood leukemia and ALL in cohort studies. However, more studies are warranted to validate these results and explore the biologic mechanisms underlying these relationships.

The Relationship of PM Variation with Visibility and Mixing-Layer Height under Hazy/Foggy Conditions in the Multi-Cities of Northeast China.

The variations of visibility, PM-mass concentration and mixing-layer height (MLH) in four major urban/industry regions (Shenyang, Anshan, Benxi and Fushun) of central Liaoning in Northeast China are evaluated from 2009 to 2012 to characterize their dynamic effect on air pollution. The annual mean visibilities are about 13.7 ± 7.8, 13.5 ± 6.5, 12.8 ± 6.1 and 11.5 ± 6.8 km in Shenyang, Anshan, Benxi and Fushun, respectively. The pollution load (PM × MLH) shows a weaker vertical diffusion in Anshan, with a higher PM concentration near the surface. High concentrations of fine-mode particles may be partially attributed to the biomass-burning emissions from September in Liaoning Province and surrounding regions in Northeast China as well as the coal burning during the heating period with lower MLH in winter. The visibility on non-hazy fog days is about 2.5-3.0 times higher than that on hazy and foggy days. The fine-particle concentrations of PM2.5 and PM1.0 on hazy and foggy days are ~1.8-1.9 times and ~1.5 times higher than those on non-hazy foggy days. The MLH declined more severely during fog pollution than in haze pollution. The results of this study can provide useful information to better recognize the effects of vertical pollutant diffusion on air quality in the multi-cities of central Liaoning Province in Northeast China.

RhoA is essential for maintaining normal megakaryocyte ploidy and platelet generation.

RhoA plays a multifaceted role in platelet biology. During platelet development, RhoA has been proposed to regulate endomitosis, proplatelet formation, and platelet release, in addition to having a role in platelet activation. These processes were previously studied using pharmacological inhibitors in vitro, which have potential drawbacks, such as non-specific inhibition or incomplete disruption of the intended target proteins. Therefore, we developed a conditional knockout mouse model utilizing the CRE-LOX strategy to ablate RhoA, specifically in megakaryocytes and in platelets to determine its role in platelet development. We demonstrated that deleting RhoA in megakaryocytes in vivo resulted in significant macrothrombocytopenia. RhoA-null megakaryocytes were larger, had higher mean ploidy, and exhibited stiff membranes with micropipette aspiration. However, in contrast to the results observed in experiments relying upon pharmacologic inhibitors, we did not observe any defects in proplatelet formation in megakaryocytes lacking RhoA. Infused RhoA-null megakaryocytes rapidly released platelets, but platelet levels rapidly plummeted within several hours. Our evidence supports the hypothesis that changes in membrane rheology caused infused RhoA-null megakaryocytes to prematurely release aberrant platelets that were unstable. These platelets were cleared quickly from circulation, which led to the macrothrombocytopenia. These observations demonstrate that RhoA is critical for maintaining normal megakaryocyte development and the production of normal platelets.