Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
Sci Rep ; 10(1): 16759, 2020 10 07.
Artículo en Inglés | MEDLINE | ID: mdl-33028865

RESUMEN

Cynomolgus monkeys (Macaca fascicularis; MF) are commonly used as nonhuman primate models for pharmaceutical product testing. In their habitat range, monkeys have close contact with humans, allowing the possibility of bidirectional transmission of tuberculosis (TB) between the two species. Although the intradermal tuberculin skin test (TST) is used for TB detection in MF, it has limitations. Herein, we established the mIGRA, combining human QuantiFERON-TB Gold-Plus and monkey IFN-γ ELISApro systems, and used it to investigate 39 captive MF who were cage-mates or lived in cages located near a monkey who died from the naturally TB infection. During a 12-month period of study, 14 (36%), 10 (26%), and 8 (21%) monkeys showed TB-positive results using the mIGRA, the TST, and TB culture, respectively. Among the 14 mIGRA-positive monkeys, 8 (57.1%) were TST-positive and 7 (50%) were culture-positive, indicating early TB detection in the latent and active TB stages with the mIGRA. Interestingly, 3 (37.5%) of the TST-negative monkeys were culture-positive. Our study showed that the mIGRA offers many advantages, including high sensitivity and high throughput, and it requires only one on-site visit to the animals. The assay may be used as a supplementary tool for TB screening in MF.


Asunto(s)
Ensayos de Liberación de Interferón gamma/veterinaria , Tuberculosis Latente/veterinaria , Prueba de Tuberculina/veterinaria , Tuberculosis/veterinaria , Animales , Tuberculosis Latente/diagnóstico , Macaca fascicularis , Tuberculosis/diagnóstico
2.
Mol Microbiol ; 36(5): 1156-66, 2000 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10844699

RESUMEN

The genes encoding GDP-mannose pyrophosphorylase from Saccharomyces cerevisiae (SRB1/PSA1) and Candida albicans (CaSRB1) were expressed under the control of the tightly regulated promoters of MET3 and CaMET3 respectively. Northern analysis showed that the addition of methionine effectively blocks the transcription of pMET3-SRB1/PSA1 and pCaMET3CaSRB1 expression cassettes, which had been integrated into the genomes of appropriate mutants. Methionine-mediated repression of CaSRB1 caused loss of viability in C. albicans, demonstrating that, as in S. cerevisiae, the gene is essential for growth. Depletion of GDP-mannose pyrophosphorylase had a highly pleiotropic effect in the two yeasts. The major phenotypes observed were lysis, failure of cell separation and/or cytokinesis, impaired bud growth and bud's site selection, clumping and flocculation, as well as increased sensitivity to a wide range of antifungal drugs and cell wall inhibitors, and impaired hyphal switching ability. These phenotypes resulted from defects in glycosylation, as demonstrated by reduced affinity for Alcian blue and sensitivity to hygromycin B. Our results provide new information about the roles of protein glycosylation in yeast and, in particular, the steps that require GDP-mannose in the fungal pathogen C. albicans.


Asunto(s)
Candida albicans/enzimología , Nucleotidiltransferasas/fisiología , Saccharomyces cerevisiae/enzimología , Candida albicans/genética , Candida albicans/crecimiento & desarrollo , Glicosilación , Mutagénesis , Nucleotidiltransferasas/genética , Fenotipo , Saccharomyces cerevisiae/genética
3.
Gene ; 149(2): 271-6, 1994 Nov 18.
Artículo en Inglés | MEDLINE | ID: mdl-7959001

RESUMEN

cDNA clones encoding giant catfish (Pangasianodon gigas) growth hormone (GH) have been isolated using a polymerase chain reaction (PCR) strategy. Pairwise combinations of degenerate and general primers allowed for the amplification of regions both 3' and 5' to the point of entry into the message. The amplified PCR products were cloned and sequenced. The cDNA sequence was found to encode a polypeptide of 200 amino acids (aa), including a putative signal peptide of 22 aa. The 5' and 3' untranslated regions of the message are 58 and 515 nucleotides long, respectively. The giant catfish GH displays the highest aa sequence homology with the carp GH, with 80% of sequence identity. Moreover, giant catfish GH has structural features in common with both mammalian and avian GH polypeptides, and also contains the domains of conserved sequence found in other GH.


Asunto(s)
Bagres/genética , Hormona del Crecimiento/biosíntesis , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Carpas/genética , Clonación Molecular/métodos , Cartilla de ADN , ADN Complementario , Hormona del Crecimiento/genética , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/métodos , ARN Mensajero/biosíntesis , Homología de Secuencia de Aminoácido
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...