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1.
Photodiagnosis Photodyn Ther ; 44: 103840, 2023 Dec.
Article En | MEDLINE | ID: mdl-37866443

BACKGROUND: Photodynamic therapy (PDT) is a potential treatment for port-wine stains (PWS), but its effects on intraocular pressure (IOP) have not been reported. This study evaluated the efficacy of PDT for facial PWS and analyzed the changes in IOP before and after treatment. METHODS: Data from 32 patients with facial PWS who underwent single PDT treatment at our department were collected. The patients were divided into three groups based on the location of the PWS. Group A (15 cases) involved the eyelid of the eye being measured; Group B (10 cases) was located near the eyes but did not involve the measured eyelid; and Group C (7 cases) was situated on the face but not near the eyes. IOP measurements were taken before and after treatment, and the efficacy and changes in IOP were analyzed. RESULTS: The overall efficacy rates of single PDT were 84.37 %, demonstrating superior efficacy for the pink type, age < 6 years, and skin lesions < 10 cm2 (P < 0.05). The higher IOP was observed on the side with eyelid involvement of PWS (P < 0.001). The IOP of the affected side in Group A decreased by 2.13 ± 2.10 mmHg on average after treatment, which was statistically significant compared with the other two groups (P<0.05). CONCLUSIONS: Eyelid involvement in PWS increases the risk of elevated IOP. Hemoporfin-mediated PDT can reduce the IOP in patients with PWS involving the eyelid within a safe range. PDT for facial PWS is considered to be safe and effective.


Glaucoma , Photochemotherapy , Port-Wine Stain , Humans , Child , Photosensitizing Agents/pharmacology , Photosensitizing Agents/therapeutic use , Port-Wine Stain/drug therapy , Port-Wine Stain/pathology , Photochemotherapy/methods , Intraocular Pressure , Glaucoma/drug therapy
2.
Methods Mol Biol ; 2111: 21-33, 2020.
Article En | MEDLINE | ID: mdl-31933195

The recent launch of mass cytometry or cytometry by time of flight (CyTOF) has revolutionized flow cytometry. Similar to fluorescence flow cytometry, a key challenge for CyTOF is to analyze samples of limited amount or very rare cell populations under various experimental settings. Here we describe a carrier strategy that significantly reduces the required sample amount without losing analytical resolution. We were able to detect as few as 5 × 104 human peripheral blood mononuclear cells (PBMCs) using this method. This simple method thus enables the maximal usage of valuable clinical samples.


Antibodies/metabolism , Flow Cytometry/instrumentation , Leukocytes, Mononuclear/immunology , Metals, Heavy/chemistry , Animals , Antibodies/chemistry , Cell Line , Humans , Leukocyte Count , Mice , Staining and Labeling
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