RESUMEN
The effects of fractions partitioned from the ethanol extract of Acorus calamus L. (AC) on adipocyte differentiation were investigated using cultured mouse 3T3-L1 preadipocytes. The degree of differentiation was evaluated by measuring the cellular triglycerides and protein expression of the glucose transporter GLUT4 in 3T3-L1 cells. The ethyl acetate fraction of the AC extract (ACE) was found to enhance adipocyte differentiation as did rosiglitazone. The results of further fractionation of ACE indicated that the active fraction does not consist of beta-asarone, which is a toxic component of this plant. This finding suggests that ACE has potential insulin-sensitizing activity like rosiglitazone, and may improve type 2 diabetes.
Asunto(s)
Acorus/química , Adipocitos/efectos de los fármacos , Anisoles/química , Diferenciación Celular/efectos de los fármacos , Extractos Vegetales/farmacología , Células 3T3-L1 , Acetatos/química , Adipocitos/citología , Adipocitos/metabolismo , Derivados de Alilbenceno , Animales , Relación Dosis-Respuesta a Droga , Transportador de Glucosa de Tipo 4/metabolismo , Ratones , Estructura Molecular , Extractos Vegetales/química , Rosiglitazona , Tiazolidinedionas/farmacología , Triglicéridos/metabolismoRESUMEN
OBJECTIVE: To isolate and determine the chemical constituents from Ranunculus japonicus. METHOD: Compounds were isolated by column chromatography, and identified by MS, NMR data. RESULT: Nine compounds were obtained andelucidated as scoparone (1), tricin (2), protocatechuic acid (3), luteolin (4), anemonin (5), scopoletin (6), 5-hydroxy-6, 7-dimethoxyflavone (7), 5-hydroxy-7, 8-dimethoxyflavone (8), ternatolide (9), respectively. CONCLUSION: Compounds 1-9 were isolated from R. japonicus for the first time while compounds 7, 8 were separated from Ranunculus genus first time.