RESUMEN
In rodent models of traumatic brain injury (TBI), both Interleukin-1ß (IL-1ß) and tumor necrosis factor-α (TNFα) levels increase early after injury to return later to basal levels. We have developed and characterized a rat mild fluid percussion model of TBI (mLFP injury) that results in righting reflex response times (RRRTs) that are less than those characteristic of moderate to severe LFP injury and yet increase IL-1α/ß and TNFα levels. Here we report that blockade of IL-1α/ß and TNFα binding to IL-1R and TNFR1, respectively, reduced neuropathology in parietal cortex, hippocampus, and thalamus and improved outcome. IL-1ß binding to the type I IL-1 receptor (IL-1R1) can be blocked by a recombinant form of the endogenous IL-1R antagonist IL-1Ra (Kineret). TNFα binding to the TNF receptor (TNFR) can be blocked by the recombinant fusion protein etanercept, made up of a TNFR2 peptide fused to an Fc portion of human IgG1. There was no benefit from the combined blockades compared with individual blockades or after repeated treatments for 11 days after injury compared with one treatment at 1 hr after injury, when measured at 6 hr or 18 days, based on changes in neuropathology. There was also no further enhancement of blockade benefits after 18 days. Given that both Kineret and etanercept given singly or in combination showed similar beneficial effects and that TNFα also has a gliotransmitter role regulating AMPA receptor traffic, thus confounding effects of a TNFα blockade, we chose to focus on a single treatment with Kineret.
Asunto(s)
Antiinflamatorios no Esteroideos/uso terapéutico , Lesiones Encefálicas/tratamiento farmacológico , Lesiones Encefálicas/metabolismo , Receptores de Citocinas/metabolismo , Animales , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Lesiones Encefálicas/patología , Proteínas de Unión al Calcio/metabolismo , Modelos Animales de Enfermedad , Etanercept/uso terapéutico , Regulación de la Expresión Génica/efectos de los fármacos , Proteína Antagonista del Receptor de Interleucina 1/uso terapéutico , Masculino , Proteínas de Microfilamentos/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Actividad Motora/efectos de los fármacos , Proteína Básica de Mielina/metabolismo , Vaina de Mielina/efectos de los fármacos , Vaina de Mielina/patología , Fosfopiruvato Hidratasa/metabolismo , Ratas , Ratas Sprague-Dawley , Reflejo/efectos de los fármacos , Reflejo/fisiología , Factores de TiempoRESUMEN
There are age-associated cognitive and cholinergic deficits in the neurotrophin-dependent cholinergic basal forebrain neurons (CBFNs). There are also increases in the activity of the transcription factor NF-kappaB in the aged rodent brain that may reflect chronic enhancement of stress response signaling. We used partial immunolesions (PIL) to CBFN to examine the role of endogenous NGF on choline acetyltransferase (ChAT) activity and NGF-mediated NF-kappaB alteration after cholinergic deafferentation. We injected 192 IgG-saporin, an immunotoxin selectively taken up by neurotrophin receptor p75(NTR)-bearing neurons, into lateral ventricles, followed by infusions of anti-NGF to assess NF-kappaB, ChAT and NGF responses to PIL after anti-NGF infusion. Treatment with anti-NGF decreased ChAT activity by 17-34% in the cortex, hippocampus, and olfactory bulb and PIL decreased ChAT activity by 47-73%. Changes in AChE activity levels paralleled those observed for ChAT after PIL. NGF protein levels in the olfactory bulb, but not the cortex or hippocampus, increased significantly after PIL treatment. Infusion of anti-NGF abolished the PIL-induced eight-fold NGF increase in CNS. NF-kappaB binding activity to the IgG-kappaB and ChAT specific NF-kappaB consensus sequences, increased in the cortex but not hippocampus after PIL followed by anti-NGF infusion. It is likely that immunolesion-induced changes in ambient NGF levels may perturb NF-kappaB activity.
Asunto(s)
Colina O-Acetiltransferasa/metabolismo , FN-kappa B/metabolismo , Factor de Crecimiento Nervioso/metabolismo , Neuronas/enzimología , Acetilcolinesterasa/metabolismo , Animales , Anticuerpos Monoclonales/farmacología , Biomarcadores , Colinérgicos/farmacología , Proteínas de Unión al ADN/metabolismo , Desnervación , Electroforesis , Inmunotoxinas/farmacología , Microinyecciones , N-Glicosil Hidrolasas , Factor de Crecimiento Nervioso/inmunología , Prosencéfalo/citología , Unión Proteica/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Proteínas Inactivadoras de Ribosomas Tipo 1 , SaporinasRESUMEN
Cholinergic basal forebrain neurons (CBFNs) retrogradely transport neurotrophins released in the hippocampus and cortex as part of a general response to injury in a process that is impaired in the aged rodent and can be spared by the exogenous addition of pharmacological doses of nerve growth factor (NGF). This observation suggests that components of stress response signal transduction pathways in the aged CNS can be exogenously activated. The extent and mechanism of the endogenous stimulation of NGF in response to injury can be mimicked via treatment with 192 IgG-saporin of rat CNS, an immunolesion model. Here we report on the use of a conditioning lesion paradigm to determine if repeated partial immunolesions have a conditioning effect on the immunolesion-induced increases in NGF protein or decreases in choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity. We report that chronic repeated immunolesions, as used here, were not as effective as a one time equivalent immunolesion in terms of induced NGF protein increases or decreasing ChAT and AChE activity in the hippocampus and cortex. Thus, chronic lesions resulting in cholinergic impairment typical of the aged CNS may differ from acute toxic models as a result of desensitization due to a conditioning effect of chronic subthreshold lesioning events in the CNS.
Asunto(s)
Adaptación Fisiológica/efectos de los fármacos , Anticuerpos Monoclonales/farmacología , Corteza Cerebral/fisiopatología , Colinérgicos/farmacología , Hipocampo/fisiopatología , Inmunotoxinas/farmacología , Estrés Fisiológico/fisiopatología , Acetilcolinesterasa/análisis , Acetilcolinesterasa/metabolismo , Adaptación Fisiológica/inmunología , Envejecimiento/fisiología , Animales , Núcleo Basal de Meynert/citología , Corteza Cerebral/citología , Colina O-Acetiltransferasa/análisis , Colina O-Acetiltransferasa/metabolismo , Banda Diagonal de Broca/citología , Hipocampo/citología , Masculino , N-Glicosil Hidrolasas , Degeneración Nerviosa/inducido químicamente , Degeneración Nerviosa/fisiopatología , Factor de Crecimiento Nervioso/farmacología , Neuronas/química , Neuronas/enzimología , Neuronas/inmunología , Células PC12 , Ratas , Ratas Sprague-Dawley , Receptor de Factor de Crecimiento Nervioso/análisis , Proteínas Inactivadoras de Ribosomas Tipo 1 , Saporinas , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Estrés Fisiológico/inmunologíaRESUMEN
Oxidative stress has been linked to neuronal cell death resulting from either acute insults due to ischemia, trauma, excitotoxicity, or chronic neurodegenerative diseases. Cholinergic basal forebrain neurons (CBFNs) compete for nerve growth factor (NGF) synthesized in the hippocampus and cortex via retrograde transport. NGF affects CBFN survival and cholinergic function via activation of the NF-kappaB transcription factor and this signaling pathway appears to be impaired in aged rats. Here, we demonstrate that activation of NF-kappaB in basal forebrain primary culture via treatment with hydrogen peroxide or TNF-alpha is predominantly restricted to CBFNs, and that NF-kappaB activation appears to mostly affect p65 translocation to the nucleus, but not the p50 subunit. These results are consistent with NF-kappaB activation being a part of recovery processes after acute oxidative stress. Since p50 or p49 (also called p52) binding to promoter sites does not stimulate transcription - both p50 and p49 lack an activating domain - and p65 does contain an activating domain and thus can act as a transcription enhancer, differential translocation of different NF-kappaB dimers can act as repressors of constitutive activity or enhancers. These results are in agreement with the hypothesis that p50/p65 is the active trans-activating species of NF-kappaB, as compared to p50/p50 homodimers which bind to NF-kappaB binding sites but do not trans-activate promoters. Our results also suggest that selective activation of different NF-kappaB dimer species may have regulatory significance in neuronal responses to acute or chronic insults to CNS. Thus, increased p65 translocation could have enhancing effects while increased p50 translocation could have a repressor role. Manipulation of the types of NF-kappaB species being translocated could provide a basis for therapeutic strategies.
Asunto(s)
FN-kappa B/metabolismo , Neuronas/metabolismo , Estrés Oxidativo/fisiología , Prosencéfalo/citología , Acetilcolinesterasa/análisis , Animales , Anticuerpos , Astrocitos/química , Células Cultivadas , Fibras Colinérgicas/enzimología , Femenino , Proteína Ácida Fibrilar de la Glía/análisis , Peróxido de Hidrógeno/farmacología , FN-kappa B/análisis , FN-kappa B/inmunología , Factor de Crecimiento Nervioso/farmacología , Neuronas/efectos de los fármacos , Neuronas/ultraestructura , Oxidantes/farmacología , Estrés Oxidativo/efectos de los fármacos , Embarazo , Ratas , Ratas Sprague-Dawley , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
Rat pheochromocytoma (PC12) cells exhibit apoptotic cell death when deprived of serum and can be rescued by nerve growth factor (NGF). We characterized AP-1 DNA binding activity in PC12 cells after serum deprivation in the presence or absence of NGF or other neurotrophic agents. There was a decline in AP-1 DNA binding activity concomitant with apoptosis in PC12 cells after serum deprivation. Treatment of serum-deprived PC12 with NGF induced persistent AP-1 binding activity that was blocked by the Trk receptor inhibitor K252a. PC12 cells treated with dibutyryl cyclic AMP or insulin also displayed increased AP-1 DNA binding activity. While NGF somewhat increased c-Fos and c-Jun protein levels transiently, it had a more robust and persistent stimulatory effect on Jun B protein levels. AP-1 transcriptional activity increased after NGF, dibutyryl cAMP, or insulin treatment under serum free conditions. Curcumin, which inhibits AP-1 activity, blocked the NGF-mediated rescue. These results would suggest that the rescue of serum-deprived PC12 cells from apoptosis requires increasing endogenous levels of specific Fos/Jun components of AP-1.
Asunto(s)
Apoptosis , Factor de Crecimiento Nervioso/farmacología , Células PC12/metabolismo , Factor de Transcripción AP-1/metabolismo , Animales , Apoptosis/efectos de los fármacos , Bucladesina/farmacología , Carbazoles/farmacología , Medio de Cultivo Libre de Suero , Curcumina/farmacología , ADN/metabolismo , Expresión Génica/efectos de los fármacos , Genes jun/genética , Alcaloides Indólicos , Insulina/farmacología , Células PC12/citología , Proteína Quinasa C/antagonistas & inhibidores , RatasRESUMEN
Apoptosis in the nervous system is a necessary event during the development of the nervous system and is also present after genotoxic events, be they chronic as in aging or more acute after trauma and ischemia. Apoptotic events reflect an interplay between intrinsic signaling events that rely on cytokines, neurotransmitters, and growth factors and responses to extrinsic events that increase levels of radical oxygen species. Both intrinsically and extrinsically driven signal-transduction pathways act via transcription factors that regulate the coordinated timely expression of stress-response genes as part of a decision-making process that can commit cells to apoptosis or survival. Here we discuss the role of two transcription factors that participate in apoptosis in the nervous system: the activator protein AP-1 and nuclear factor kappaB.
Asunto(s)
Apoptosis/fisiología , Degeneración Nerviosa/fisiopatología , Neuronas/citología , Transducción de Señal/fisiología , Animales , HumanosRESUMEN
PC12 cells are known to undergo programmed cell death (apoptosis) when they are deprived of serum. Nerve growth factor (NGF) rescues PC12 cells from serum deprivation-induced apoptosis. In the present study, we examined the effects of retinoic acid (RA), a classic morphogen, on apoptosis in PC12 cells after serum deprivation and NGF-mediated rescue. In naive PC12 cells, all trans-RA treatment induced cell death in the presence of NGF. RA also abolished the protective effects of dibutyryl cyclic AMP or insulin under serum-free conditions. The death process was accompanied by nuclear condensation and DNA fragmentation, typical of apoptosis. In addition, RA also increased the extent of apoptosis in PC12 cells after serum deprivation. Cycloheximide, an inhibitor of protein synthesis, did not abolish the effects of RA on serum-deprived PC12 cells. RA also decreased thymidine incorporation and proliferation in NGF-treated PC12 cells. Furthermore, although the total DNA binding activity of the AP-1 transcription factor was not changed after RA treatment, RA decreased a specific AP-1 transcriptional activity. It is surprising that differentiated PC12 cells resisted the toxic effects of RA. These data suggest that RA might function as an endogenous inducer of apoptosis during neural differentiation by a mechanism distinct from that of serum deprivation.
Asunto(s)
Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Factores de Crecimiento Nervioso/farmacología , Células PC12/efectos de los fármacos , Tretinoina/farmacología , Animales , Proteínas Sanguíneas/farmacología , Diferenciación Celular/efectos de los fármacos , División Celular/efectos de los fármacos , Núcleo Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Medio de Cultivo Libre de Suero/farmacología , ADN/efectos de los fármacos , ADN/metabolismo , Fragmentación del ADN , Células PC12/citología , Células PC12/fisiología , Ratas , Factor de Transcripción AP-1/efectos de los fármacos , Factor de Transcripción AP-1/metabolismo , Transcripción Genética/efectos de los fármacosRESUMEN
The aim of the present study was to examine the effects of nerve growth factor on brain cholinergic function after a partial immunolesion to the rat cholinergic basal forebrain neurons (CBFNs) by 192 IgG-saporin. Two weeks after intraventricular injections of 1.3 micrograms of 192 IgG-saporin, about 50% of CBFNs were lost which was associated with 40-60% reductions of choline acetyltransferase (ChAT) and high-affinity choline uptake (HACU) activities throughout the basal forebrain cholinergic system. Two groups of lesioned animals received intraventricular transplantations of mouse 3T3 fibroblasts retrovirally transfected with either the rat NGF gene (3T3NGF+) or the retrovirus alone (3T3NGF-) and were sacrificed eight weeks later. In vivo production of NGF by 3T3NGF+ cells was confirmed by NGF immunohistochemistry on the grafts and NGF immunoassay on cerebrospinal fluid (CSF) samples. Both ChAT and HACU activities returned to normal control levels in the basal forebrain and cortex after 3T3NGF+ transplants, whereas no recovery was observed in 3T3NGF- transplanted animals. There was a 25% increase in the size of remaining CBFNs and an increased staining intensity for NGF immunoreactivity in these cells after NGF treatments. Acetylcholinesterase (AChE) histochemistry revealed that the optical density of AChE-positive fibers in the cerebral cortex and hippocampus were reduced by about 60% in immunolesioned rats which were completely restored by 3T3NGF+ grafts. In addition, decreases in growth-associated protein (GAP)-43 immunoreactivity after immunolesion and increases in synaptophysin immunoreactivity after 3T3NGF+ grafts were observed in the hippocampus. Our results further confirm the notion that transfected NGF-secreting cells are useful in long-term in vivo NGF treatment and NGF can upregulate CBFN function. They also highly suggest that NGF induces terminal sprouting from remaining CBFNs.
Asunto(s)
Trasplante de Células/fisiología , Ventrículos Cerebrales/fisiología , Factores de Crecimiento Nervioso/metabolismo , Neuronas/fisiología , Sistema Nervioso Parasimpático/citología , Prosencéfalo/citología , Células 3T3 , Animales , Anticuerpos Monoclonales/toxicidad , Ventrículos Cerebrales/citología , Colina/metabolismo , Colina O-Acetiltransferasa/metabolismo , Colinérgicos/toxicidad , Proteína GAP-43 , Inmunotoxinas/toxicidad , Masculino , Glicoproteínas de Membrana/biosíntesis , Ratones , N-Glicosil Hidrolasas , Factores de Crecimiento Nervioso/genética , Proteínas del Tejido Nervioso/biosíntesis , Sistema Nervioso Parasimpático/fisiología , Prosencéfalo/fisiología , Ratas , Ratas Sprague-Dawley , Proteínas Inactivadoras de Ribosomas Tipo 1 , Saporinas , Sinaptofisina/biosíntesisRESUMEN
Programmed cell death, the intrinsic form of apoptosis, plays an integral role in those neurodegenerative events associated with age-related neuropathology. Neurotrophins (NTs), such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and NT-3, are required for survival of certain neurons, and thus their clinical use to counteract age- and pathology-associated neurodegeneration has been suggested, although mechanistic descriptions for NT cell rescue from apoptosis are not definitive. Here we attempted to isolate the individual actions of high-affinity tyrosine kinase (Trk) receptors and p75NGFR, the common low-affinity NT receptor, in NT rescue of apoptotic PC12 cells. Our results showed that whereas inhibiting Trk receptor phosphorylation abolishes NGF rescue of serum-deprived PC12 cells from apoptosis, TrkA suppression with antisense oligonucleotides did not. Also, although BDNF did not rescue naive serumless PC12 cells, which lack the BDNF-specific TrkB receptor, it significantly increased survival of TrkA-suppressed serum-starved PC12 cells. These data confirm the hypothesis that binding of any NT to Trk-free p75NGFR-bearing cells blocks apoptosis but also suggest that if Trk receptors are expressed, prohibiting Trk phosphorylation also blocks NT-mediated rescue from apoptosis.
Asunto(s)
Factores de Crecimiento Nervioso/fisiología , Células PC12/fisiología , Proteínas Proto-Oncogénicas/antagonistas & inhibidores , Proteínas Tirosina Quinasas Receptoras/antagonistas & inhibidores , Receptores de Factor de Crecimiento Nervioso/antagonistas & inhibidores , Animales , Apoptosis/fisiología , Secuencia de Bases , Factor Neurotrófico Derivado del Encéfalo/farmacología , Carbazoles/farmacología , Supervivencia Celular/efectos de los fármacos , Medio de Cultivo Libre de Suero , Alcaloides Indólicos , Datos de Secuencia Molecular , Factores de Crecimiento Nervioso/farmacología , Neurotrofina 3 , Oligonucleótidos Antisentido/genética , Oligonucleótidos Antisentido/farmacología , Células PC12/efectos de los fármacos , Ratas , Receptor trkARESUMEN
We used the patch clamp technique to study the effect of acetyl-L-carnitine arginyl amide (ALCAA) and of nerve growth factor (NGF) on availability of L-type Ca2+ channels in rat pheochromocytoma (PC12) cells maintained in defined medium. Channel availability was measured as number of channels in the patch x the probability of opening (n.Po). In patches from control cells, cells exposed to NGF (10 ng/ml) for six days, and cells exposed to ALCAA (1 mM) for six days, n.Po, measured during 200-240 ms pulses to -10 mV (holding potential, -60 mV), was 0.102 +/- 0.089 (5 cells), 0.173 +/- 0.083 (5 cells), and 0.443 +/- 0.261 (7 cells), respectively. The 4.3-fold increase for the ALCAA-treated cells was significantly different from control (P < 0.05), whereas that for the NGF-treated cells was not. For the same conditions, the maximum number of superimposed openings at -10 mV was 1.3 +/- 0.5 (6 cells), 1.6 +/- 0.5 (8 cells), and 3.3 +/- 1.8 (8 cells), with the value for the ALCAA-treated cells being significantly different from control (P < 0.001). Additional analysis showed that the distribution of channel open times, the time constants, and the voltage dependence of activation were not changed by prolonged exposure to ALCAA. Short-term exposure to both ALCAA as well as to the parent compound, acetyl-L-carnitine (ALCAR), did not cause an increase but rather a decrease in n.Po, and this short-term effect of both compounds was blocked by neomycin, an inhibitor of phospholipase C.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Canales de Calcio/efectos de los fármacos , Carnitina/farmacología , Células PC12/metabolismo , Animales , Carnitina/análogos & derivados , Células Cultivadas , Potenciales Evocados , Neomicina/farmacología , Técnicas de Placa-Clamp , RatasRESUMEN
Senescence of the central nervous system is characterized by a progressive loss of neurons that can result in physiological and behavioral impairments. Reduction in the levels of central neurotrophic factors or of neurotrophin receptors may be one of the causes of the onset of these degenerative events. Thus, a proper therapeutic approach would be to increase support to degenerating neurons with trophic factors or to stimulate endogenous neurotrophic activity. Here we report that acetyl-L-carnitine arginine amide (ST-857) is able to stimulate neurite outgrowth in rat pheochromocytoma PC12 cells in a manner similar to that elicited by nerve growth factor (NGF). Neurite induction by ST-857 requires de novo mRNA synthesis and is independent of the action of several common trophic factors. The integrity of the molecular structure of ST-857 is essential for its activity, as the single moieties of the molecule have no effect on PC12 cells, whether they are tested separately or together. Also, minor chemical modifications of ST-857, such as the presence of the arginine moiety at a position other than the amino one, completely abolish its neuritogenic effect. Lastly, the presence of ST-857 in the culture medium competes with the high affinity NGF binding in a dose dependent fashion. These results, although preliminary, are suggestive of a possible role for ST-857 in the development of therapeutic strategies to counteract degenerative diseases of the CNS.
Asunto(s)
Acetilcarnitina/análogos & derivados , Arginina/análogos & derivados , Factores de Crecimiento Nervioso/farmacología , Neuritas/efectos de los fármacos , Receptores de Factor de Crecimiento Nervioso/efectos de los fármacos , Acetilcarnitina/farmacología , Animales , Arginina/farmacología , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Colina O-Acetiltransferasa/metabolismo , Factores de Crecimiento Nervioso/metabolismo , Células PC12 , Ratas , Receptores de Factor de Crecimiento Nervioso/metabolismo , Estimulación Química , Transcripción Genética/efectos de los fármacosRESUMEN
Nerve growth factor (NGF) is a member of the neurotrophin family and is required for the survival and maintenance of peripheral sympathetic and sensory ganglia. In the CNS, NGF regulates cholinergic expression by basal forebrain cholinergic neurons. NGF also stimulates cellular resistance to oxidative stress in the PC12 cell line and protects PC12 cells from the toxic effects of reactive oxygen species. The hypothesis that NGF protection involves changes in antioxidant enzyme expression was tested by measuring its effects on catalase and glutathione peroxidase (GSH Px) mRNA expression in PC12 cells. NGF increased catalase and GSH Px mRNA levels in PC12 cells in a time- and dose-dependent manner. There was also a corresponding increase in the enzyme activities of catalase and GSH Px. Thus, NGF can provide cytoprotection to PC12 cells by inducing the free radical scavenging enzymes catalase and GSH Px.
Asunto(s)
Catalasa/metabolismo , Glutatión Peroxidasa/metabolismo , Factores de Crecimiento Nervioso/farmacología , Células PC12/metabolismo , Animales , Catalasa/genética , Relación Dosis-Respuesta a Droga , Glutatión Peroxidasa/genética , ARN Mensajero/metabolismo , RatasRESUMEN
Our hypothesis is that one cause of neuronal cell death and shrinkage in the aged central nervous system is an inability of neurons to maintain oxidant homeostasis in the face of increased levels of reactive oxygen species, decreased endogenous antioxidants, and impaired energy metabolism associated with physiological senescence, Alzheimer's, and Parkinson's diseases. Since treatment with nerve growth factor (NGF) reverses behavioral impairments in aged rats and stimulates cholinergic activity in the basal forebrain, while brain-derived neurotrophic factor appears to play a similar role in the striatum, we propose that neurotrophin-mediated cell-sparing reflects effects on oxidant homeostasis. Neurotrophins may play a similar cell-sparing role in hypoxic/ischemic injury to the nervous system, which also is mediated in part by reactive oxygen species. The degradation of one such species, H2O2, is catalyzed by catalase and glutathione peroxidase (GSH Px). The activity of the latter enzyme is dependent on glutathione reductase and the availability of NADPH for regeneration of reduced GSH. The GSH redox cycle is also regulated by enzymes of the hexose monophosphate shunt. NGF protects PC12 cells from H2O2 injury by stimulating the synthesis of antioxidant enzymes including catalase, GSH Px, glucose-6-phosphate dehydrogenase, and gamma-glutamylcysteine synthetase, the rate-limiting enzyme for glutathione synthesis. NGF also enhances recovery from the NAD+ losses occurring as a consequence of H2O2 treatment.
Asunto(s)
Sistema Nervioso Central/fisiología , Metabolismo Energético/fisiología , Homeostasis/fisiología , Neurotransmisores/fisiología , Animales , Sistema Nervioso Central/metabolismo , HumanosRESUMEN
Stepwise selection in increasing H2O2 concentrations was used to obtain a PC12 cell variant designated HPR. This variant was stably resistant to H2O2 as compared with the parental PC12 cell line. HPR cells responded to nerve growth factor (NGF) by further enhancing H2O2 resistance. This variant was subcloned by limiting dilution to obtain the line referred to as HPR-C, which was stably resistant to H2O2 toxicity and retained NGF responses, including morphologic changes and further reduction of H2O2 toxicity. When compared with the parental PC12 line, the HPR-C subclone did not have higher levels of catalase or glutathione peroxidase (GSH Px) activity or mRNA expression (as assessed by PCR analysis of cDNA reverse transcribed from total cellular RNA). HPR-C cells retained the ability to respond to NGF treatment by increasing catalase and GSH Px activity and expression. These data suggest that the protective effects of conditioning lesions, unlike those of neurotrophins, are in part independent of changes in the activity or expression of antioxidant enzymes.
Asunto(s)
Neoplasias de las Glándulas Suprarrenales/tratamiento farmacológico , Catalasa/efectos de los fármacos , Glutatión Peroxidasa/efectos de los fármacos , Factores de Crecimiento Nervioso/farmacología , Feocromocitoma/tratamiento farmacológico , Neoplasias de las Glándulas Suprarrenales/enzimología , Animales , Supervivencia Celular/efectos de los fármacos , Resistencia a Medicamentos/fisiología , Peróxido de Hidrógeno , Células PC12 , Feocromocitoma/enzimología , RatasRESUMEN
Ionic channels in human cortical neurons have not been studied extensively. HCN-1 and HCN-1A cells, which recently were established as continuous cultures from human cortical tissue, have been shown by histochemical and immunochemical methods to exhibit a neuronal phenotype, but expression of functional ionic channels was not demonstrated. For the present study, HCN-1 and HCN-1A cells were cultured in Dulbecco's modified Eagle's medium with 15% fetal calf serum, in some cases supplemented with 10 ng/ml nerve growth factor, 10 microM forskolin, and 1 mM dibutyryl cyclic adenosine monophosphate to promote differentiation. Cells or membrane patches were voltage clamped using conventional patch clamp techniques. In HCN-1A cells, we identified a tetrodotoxin-sensitive Na+ current, two types of Ca2+ channel current, including L-type current and a second type that in some respects resembled N-type current, and four types of K+ current, including a delayed outward rectifier that showed voltage-dependent inactivation, two types of noninactivating Ca(2+)-activated K+ channels with slope conductances of 146 and 23 pS (K+i/K+o 145 mM/5 mM), and less frequently, a noninactivating, intermediate conductance channel that was not sensitive to internal Ca2+. When HCN-1A cells were examined after 3 days of exposure to differentiating agents, pronounced morphological changes were evident but no differences in ionic currents were apparent. HCN-1 cells also exhibited K+ and Ca2+ channel currents, but Na+ currents were not detected in these cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Corteza Cerebral/metabolismo , Canales Iónicos/efectos de los fármacos , Neuronas/metabolismo , Canales de Calcio/efectos de los fármacos , Canales de Calcio/metabolismo , Diferenciación Celular/efectos de los fármacos , Células Cultivadas , Corteza Cerebral/citología , Electrofisiología , Humanos , Canales de Potasio/efectos de los fármacos , Canales de Potasio/metabolismo , Canales de Sodio/efectos de los fármacos , Canales de Sodio/metabolismoRESUMEN
The HCN-1A clonal cell line, derived from the cortical tissue of a patient with unilateral megencephaly, was shown to differentiate into a mature neuronal-like state in the presence of the nerve growth factor, dibutyryl cyclic adenosine, 3',5'-monophosphate and either 1-isobutyl-3-methylxanthine or forskolin. Differentiation was assessed by measuring the percentage of cells that displayed branched, varicose processes that stained for synaptophysin. Treatment of cultures with a cocktail containing forskolin increased immunocytochemical staining for gamma aminobutyric (GABA), neurofilament protein and the nerve growth factor receptor species p75NGFR. Treatment with acetyl-L-carnitine alone had some effects on the cell morphology while acetyl-L-carnitine arginyl amide and nerve growth factor together increased the GABA content. Positive staining levels for the neurotransmitters gamma aminobutyric acid, glutamate, somatostatin, cholecystokinin and vasoactive intestinal polypeptide were measured quantitatively for HCN-1A under basal conditions.
Asunto(s)
Acetilcarnitina/análogos & derivados , Arginina/análogos & derivados , Factores de Crecimiento Nervioso/farmacología , Neuronas/efectos de los fármacos , 1-Metil-3-Isobutilxantina/farmacología , Acetilcarnitina/farmacología , Animales , Arginina/farmacología , Química Encefálica/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Línea Celular , Colforsina/farmacología , Humanos , Inmunohistoquímica , Ratones , Proteínas de Neurofilamentos/metabolismo , Ácido gamma-Aminobutírico/inmunología , Ácido gamma-Aminobutírico/metabolismoRESUMEN
Neurotrophic factors regulate neuronal survival and neurite growth in development and following injury. Oxidative stress produced in neurons as a consequence of primary injury, or during reperfusion following ischemia, may contribute to cell death. Here, the effects of nerve growth factor (NGF) on the response to H2O2 injury were examined in the PC12 rat pheochromocytoma cell line. Specifically, the effect of NGF on cell viability after H2O2 injury was measured. Pretreatment with NGF enhanced survival after H2O2 treatment, as measured by Trypan blue dye exclusion, radiolabeled amino acid incorporation, tetrazolium salt reduction, or cytoplasmic enzyme release. One early event associated with H2O2 treatment was a rapid decrease in NAD+. Although initial decreases in NAD+ levels were similar in control and NGF-treated cells, the latter recovered more rapidly and extensively. The decline in total NAD observed after NGF treatment was almost equal in magnitude to the measured increase in NADP. Inhibition of poly(ADP-ribose) polymerase also enhanced viability following H2O2 injury. Treatment with both NGF and an inhibitor of this enzyme resulted in a greater reduction of H2O2 toxicity than was observed with either agent alone. These data suggest that NGF protection is multifactorial and that a significant component of the NGF effect is due to its regulatory role in the metabolism of pyridine nucleotides.
Asunto(s)
Muerte Celular/efectos de los fármacos , Peróxido de Hidrógeno/farmacología , NADP/metabolismo , NAD/metabolismo , Factores de Crecimiento Nervioso/farmacología , Animales , Benzamidas/farmacología , Colorantes , Espectroscopía de Resonancia Magnética , Oxidación-Reducción , Células PC12 , Poli Adenosina Difosfato Ribosa/antagonistas & inhibidores , Poli Adenosina Difosfato Ribosa/metabolismo , Ratas , Sales de Tetrazolio/metabolismo , Tiazoles/metabolismoRESUMEN
Acetyl-L-carnitine (ALCAR) prevents some deficits associated with aging in the central nervous system (CNS), such as the aged-related reduction of nerve growth factor (NGF) binding. The aim of this study was to ascertain whether ALCAR could affect the expression of an NGF receptor (p75NGFR). Treatment of PC12 cells with ALCAR increased equilibrium binding of 125I-NGF. ALCAR treatment also increased the amount of immunoprecipitable p75NGFR from PC12 cells. Lastly, the level of p75NGFR messenger RNA (mRNA) in PC12 was increased following ALCAR treatment. These results are in agreement with the hypothesis that there is a direct action of ALCAR on p75NGFR expression in aged rodent CNS.
Asunto(s)
Acetilcarnitina/farmacología , Células PC12/efectos de los fármacos , Receptores de Superficie Celular/efectos de los fármacos , Envejecimiento , Animales , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Factores de Crecimiento Nervioso/metabolismo , Células PC12/metabolismo , ARN Mensajero/aislamiento & purificación , Ratas , Receptores de Superficie Celular/aislamiento & purificación , Receptores de Superficie Celular/metabolismo , Receptores de Factor de Crecimiento NerviosoRESUMEN
We have demonstrated that treatment of rat pheochromocytoma (PC12) cells with acetyl-L-carnitine (ALCAR) stimulates the synthesis of nerve growth factor receptors (NGFR). ALCAR has also been reported to prevent some age-related impairments of the central nervous system (CNS). In particular, ALCAR reduces the loss of NGFR in the hippocampus and basal forebrain of aged rodents. On these bases, a study on the effect of NGF on the PC12 cells was carried out to ascertain whether ALCAR induction of NGFR resulted in an enhancement of NGF action. Treatment of PC12 cells for 6 days with ALCAR (10 mM) stimulated [125I]NGF PC12 cell uptake, consistent with increased NGFR levels. Also, neurite outgrowth elicited in PC12 cells by NGF (100 ng/ml) was greatly augmented by ALCAR pretreatment. When PC12 cells were treated with 10 mM ALCAR and then exposed to NGF (1 ng/ml), an NGF concentration that is insufficient to elicit neurite outgrowth under these conditions, there was an ALCAR effect on neurite outgrowth. The concentration of NGF necessary for survival of serum-deprived PC12 cells was 100-fold lower for ALCAR-treated cells as compared to controls. The minimal effective dose of ALCAR here was between 0.1 and 0.5 mM. This is similar to the reported minimal concentration of ALCAR that stimulates the synthesis of NGFR in these cells. The data here presented indicate that one mechanism by which ALCAR rescues aged neurons may be by increasing their responsiveness to neuronotrophic factors in the CNS.