The Chemical Residues in Secondary Beekeeping Products of Environmental Origin.

Natural products of bee origin, despite their complex composition and difficulties in standardization, have been of high interest among scientists representing various disciplines from basic sciences to industrial and practical implementation. As long as their use is monitored and they do not impact human health, they can be considered valuable sources of many chemical compounds and are potentially useful in medicine, food processing, nutrition, etc. However, apart from honey, the general turnover of bee products lacks precise and detailed legal requirements ensuring their quality. The different residues in these products constitute a problem, which has been reported in numerous studies. All products derived from beekeeping are made by bees, but they are also influenced by the environment. Such a dual pathway requires detailed surveillance of hazards stemming from outside and inside the apiary. This should be ensured via harmonized requirements arising from the binding legal acts, especially in international and intercontinental trade zones.

The Enterotoxin Production and Antimicrobial Resistance of Campylobacter Strains Originating from Slaughter Animals.

The pathogenicity of animal-origin Campylobacter strains, including antimicrobial resistance and enterotoxigenicity, was determined in this study. Overall, 149 Campylobacter isolates originating from cattle, swine and poultry were tested. The antimicrobial resistance profiles were examined by the diffusion disk method. The dominant resistance pattern was CIP_TET. The resistance rates for ciprofloxacin among swine, cattle and poultry isolates were 84%, 51% and 66%, respectively; for tetracycline, they were 82%, 57.1% and 76%, respectively. None of the obtained isolates was resistant to all four antimicrobials tested. The ability to produce enterotoxins was assessed by the use of a suckling mouse bioassay, with intestinal fluid accumulation as a positive result, and by CHO assay, with the elongation of cells as a positive result. The ability to produce enterotoxins was significantly higher among cattle isolates (61.2% and 71.4% positive isolates, respectively, in the bioassay and the CHO assay) than among swine (16% and 32% positive isolates, respectively) or poultry isolates (14% and 22% positive isolates, respectively). A strong positive correlation between in vitro and in vivo enterotoxicity tests was demonstrated.

Propolis Contra Pharmacological Interventions in Bees.

In addition to wax, propolis is a mixture of resins, terpenes, and etheric and aromatic oils. This composition supports its very strong biochemical activity that affects bee health. Bee colonies are externally exposed to the activity of other different pharmacologically active substances and toxic agents used in beekeeping procedures, veterinary interventions, and the environment. Even if free form common diseases, they may suffer from parasites or toxins. In any such case the abundance and variety of honeyflow, besides proper therapy, is crucial for the maintenance of bee health. Propolis itself cannot be considered as food but can be considered as micro-nutrients for bees. This is due to the fact that some of its compounds may penetrate different bee products, and this way be consumed by bees and their larvae, while stored in the hive. This perspective shows propolis as natural agent reducing the toxicity of pyrethroid acaricides, stimulating production of detoxification enzymes, enhancing the action of antibiotics, and increasing expression of genes that encode proteins responsible for detoxication. The aim of this review is to summarize current data on the possible impact on veterinary public health of the introduction into propolis of residues of pharmacological agents approved in the EU for use in the treatment of bee colonies and their environment.

High contamination rates of shoes of veterinarians, veterinary support staff and veterinary students with Clostridioides difficile spores.

Clostridioides difficile is often found in animals and their environment. However, not much has been reported on veterinary clinics environment in terms of the spore load, prevalence and PCR ribotype diversity. The aim of this study was to assess the prevalence of C. difficile on shoe soles of veterinarians, veterinary support staff and veterinary students at the Veterinary Faculty campus. Altogether, 50 shoe sole swabs were collected, and the positivity rates ranged from 86.7% in swabs from veterinarians to 100% in swabs from support staff and students. Non-toxigenic and toxigenic strains representing toxinotypes 0, IV and XIX were isolated and distributed into 17 different PCR ribotypes, most common being 010, 014/020, SLO002 and 009. PCR ribotype 010 was the most prevalent and isolated from shoe soles sampled in 6/7 areas. Students' shoes had highest ribotype diversity (15/17 PCR ribotypes) but showed a low overlap with ribotype isolated from vets and support staff shoes. Veterinary students are likely the main vectors of C. difficile spores transmissions among veterinary teaching clinics and the hospital.

Effect of Mixed Invasions of Hypoderma bovis and Ostertagia ostertagi in Cattle on Milk Yield and Contents in Polish Dairy Farms.

Wide distribution of ecto- and endoparasites in cattle is a serious problem in the sustainability of a farm, due to the negative impact on animals' health and productivity. The aim of this study was to determine the presence of antibodies against Ostertagia and Hypoderma in udder milk samples and the comparison of milk yield and content of the basic components of milk in ELISA-positive and ELISA-negative cows. Milk samples were collected from 148 lactating cows from 3 herds. Antibody detection was performed using specific ELISAs for Ostertagia ostertagi and Hypoderma bovis. Milk yield and content of protein, fat, and dry matter were studied in samples from each individual cow 11 times per year at 4 week intervals. The extensiveness of dual parasitic invasions in individual herds, estimated on the basis of udder milk testing with the ELISA test, varied and amounted to 3.22%, 11.36%, and 4.76% in the three studied herds, respectively. No antibodies were found in 61.2%, 22.7%, and 57.1% of the milk samples from the cows in each herd. The milk yield of ELISA-positive cows was significantly lower in comparison to the efficiency of ELISA-negative cows and amounted to 294 kg and even to 3672 kg of milk per year, per cow. No significant differences were found between the fat and protein contents of milk between ELISA-positive and -negative cows for both parasites.

Causes of stress and conflict in the veterinary professional workplace - a perspective from Poland.

BACKGROUND: The problems of burnout and the moral and ethical distress resulting from various kinds of conflict have been raised in the veterinary profession. However, their sources and inter-relationships have not been thoroughly recognized mainly due to the multidimensional nature of human interactions related to animal breeding, farming, welfare, prophylaxis and therapy. For the first time in Poland, an analysis of conflict and conflict-causing factors in veterinary practice has been conducted with the participation of veterinarians of various specialties and the owners of different animal species. RESULTS: Conflict in the course of work is most often experienced by young veterinarians. The problems associated with communication between veterinarians and animal owners and unforeseen random situations are the general causes of conflict. Approved Veterinarians were identified by animal owners as the most common professional group associated with the conflict experienced . CONCLUSIONS: There is a lack of professional preparation by veterinary surgeons to cope with unpredicted stressful situations at work, resulting from an absence of appropriate educational input in this area. The animal owners do not understand the role and duties of Approved Veterinarians.

Antimicrobial Resistance and Virulence-Associated Markers in Campylobacter Strains From Diarrheic and Non-diarrheic Humans in Poland.

Campylobacteriosis is one of the most common causes of bacterial gastroenteritis. However, the clinical course of the illness varies in symptoms and severity. The aim of this study was to characterize Campylobacter jejuni (34 isolates) and C. coli (9 isolates) from persons with diarrheal and non-diarrheal stools at the time of examination and fecal sampling, in Poland by using whole-genome sequencing (WGS). Multilocus sequence typing (MLST) analysis revealed a high diversity with a total of 20 sequence types (STs) among 26 Campylobacter isolates from diarrheic and 13 STs among 17 isolates from non-diarrheic persons. ST-50 and ST-257 were most common in both groups. The phenotypic resistance rate was 74.4% for ciprofloxacin, 67.4% for sulfamethoxazole/trimethoprim, 58.1% for amoxicillin, 48.8% for tetracycline, and 46.5% for ceftriaxone. Only single isolates were resistant to erythromycin, gentamicin, and amoxicillin/clavulanic acid. Overall genotypic resistance toward amoxicillin, fluoroquinolones, tetracyclines, and aminoglycosides was predicted to occur in 93.1, 67.4, 48.8, and 11.6% of the isolates, respectively. None of the isolates showed the presence of the erm(B) gene or mutation in 23S rRNA. Neither was variation found in the important target region in L4 and L22 ribosomal proteins. In regard to the CmeABC efflux pump, a set of variable mutations affecting the regulatory region was noted. All Campylobacter isolates possessed genes associated with adhesion (cadF, jlpA, porA, and pebA) and invasion (ciaB, pldA, and flaC). The type IV secretion system (T4SS) was found in isolates from both diarrheic (15.4%, CI 95%: 6.1-33.5%) and non-diarrheic (23.5%, CI 95%: 9.6-47.3%) persons. The rates of the presence of cytolethal distending toxin cdtABC gene cluster and type VI secretion system (T6SS) were higher in Campylobacter isolates obtained from persons with diarrhea (96.2%, CI 95%: 81.7-99.3% and 26.9%, CI 95%: 13.7-46.1%) compared to isolates from non-diarrheic persons (76.5%, CI 95%: 52.7-90.4% and 11.8%, CI 95%: 3.3-34.3%). The lack of statistically significant differences between two groups in tested virulence factors suggests that individual susceptibility of the host might play more determining role in the disease outcome than characteristics of the infecting strain.

Pathogenicity of Campylobacter strains of poultry and human origin from Poland.

The aim of this study was to determine the pathogenic markers associated with Campylobacter infection in humans. A total of 104 Campylobacter isolates obtained from poultry and humans were examined for the presence of nine virulence genes and their ability to adhere to, invade and produce cytotoxin were defined using HeLa cells. The diversity of the Campylobacter spp. isolates was studied based on sequencing of the SVR-region of flaA gene. Altogether 45 flaA-SVR alleles were identified among 104 Campylobacter isolates of poultry and human origin. All Campylobacter isolates possessed flaA, cadF and racR genes involved in adherence. Accordingly, all poultry and human isolates exhibited adherence towards HeLa cells at mean levels of 0.95% and 0.82% of starting viable inoculum, respectively. The genes involved in invasion (iam and pldA) and cytotoxin production (cdtA, cdtB and cdtC) were also widely distributed among the human and poultry Campylobacter isolates. Significantly higher invasiveness was observed for poultry isolates (mean level of 0.002% of starting bacterial inoculum) compared to human isolates (0.0005%). Interestingly the iam gene, associated with invasion, was more common in human (100%) than poultry (84%) isolates, and the poultry isolates lacking the iam gene showed a marked reduction in their ability to invade HeLa cells. Moreover, virB11 was present in 22% of the poultry and 70.4% of the human isolates. Strains lacking virB11 showed a slight reduction in invasion, however in the absence of iam even the poultry isolates containing virB11 were unable to invade HeLa cells. The mean cytotoxicity of Campylobacter isolates from poultry and human was 26.7% and 38.7%, respectively. Strains missing both the cdtB and cdtC genes were non-cytotoxic compared to strains containing all three cdtABC genes, which were the most cytotoxic among the C. jejuni and C. coli isolates from both sources. No cytotoxic effect was observed in only 4% of poultry and 5.6% of human isolates.

Short communication: Occurrence and differentiation of Mycobacterium avium ssp. paratuberculosis (MAP) strains from milk of cows from herd with low prevalence of MAP.

Mycobacterium avium ssp. paratuberculosis (MAP) is an important pathogen responsible for the chronic progressive granulomatous enteritis known as paratuberculosis. None of the detection methods of MAP infection based on isolation of the bacterium is 100% sensitive or specific. In this article, we describe the comparison of 2 MAP detection methods: direct isolation of genetic material and culture, in individual and pooled milk samples. The genetic types of MAP detected in the samples were also identified. The study was performed in a herd of 321 cows; apparent herd seroprevalence was 3.43%. Seven of 11 individual milk samples from seropositive cows were positive by culture (and confirmed by PCR), whereas all 11 were positive by direct PCR. Of the 62 milk pools from seronegative animals, 15 were positive by culture (and confirmed by PCR) and 13 were positive by direct PCR. Using multiplex PCR and PCR-restriction enzyme analysis (PCR-REA) methods, C (cattle) and S (sheep)-types of mycobacteria were identified. Most of the genetic material tested belonged to C-type. Detection of the MAP type occurring in an infected herd can help track the source of infection. We suggest using genetic material isolated directly from pooled milk samples for quick diagnosis, identification of MAP type, and tracking of infection, without the need to sequence the entire genome.

Analysis of the Factors Influencing Veterinary Food Inspectors in Poland.

The evaluation of the quality of Veterinary Inspection in Poland has received much attention in the past few years. Veterinarians working as Food Inspectors face numerous and newly arising problems in the protection of animal health status, providing surveillance information on the occurrence of diseases, and carrying out risk analyses of the hazards related to food of animal origin. From the 130 active veterinarians attending the post graduate courses in "Hygiene of slaughter animals, meat and animal origin products" in Poland, 119 took part in the survey. The questionnaire consisted of 15 questions that delivered information on demographic features of the respondents, and various aspects of the course of their work: (a) Motivation to undertake work in food safety sector, (b) overall job satisfaction, (c) crucial negative factors and (d) occupational hazards. Participants were mainly under 40 years of age. They were Veterinary Food Inspectors working as Official and Approved Veterinarians. Permanent position and economic reasons were their main motivation in the food safety sector. They indicated problems related to insufficient training in ante and post mortem examination, work with legal acts and risk analysis. They also declared a lack of preparation in coping with crisis situations. One third of the respondents declared their health and lives were endangered, while fulfilling professional duties and pointed at different sources of hazards. The overall evaluation of the work in food safety sector was rated good and satisfactory.

Antimicrobial Resistance and Virulence Properties of Campylobacter Spp. Originating from Domestic Geese in Poland.

A total of 240 samples were evaluated for the presence of Campylobacter spp. Campylobacter was found in 83.3% of the cecum contents samples and 52.5% of the neck skin samples from carcasses. The prevailing species was C. jejuni, accounting for 87.7% of all Campylobacter isolates, and the remaining 12.3% of isolates were C. coli. All Campylobacter isolates, independent of the sample origin and species, were positive for 6 out of 15 tested genes (flaA, flhA, cadF, racR, ciaB, and cdtA genes). The prevalence of dnaJ, docA, pldA, cdtB, cdtC, and iam genes was also very common (ranging from 86.5% to 98.8%). The lowest prevalence was noted for virB11 and wlaN genes, both in Campylobacter isolates from cecum (12% and 19%) and carcasses (11.1% and 17.5%). None of the isolates tested, regardless of the sample origin, carried the cgtB gene. The highest resistance rates were observed for quinolones (90.8%) and tetracyclines (79.8%). Simultaneously, only single Campylobacter isolate was resistant to macrolides (0.6%) and none of the isolates showed resistance to aminoglycosides and amphenicols. The common presence of Campylobacter on geese carcasses as well as the detection of multidrug-resistant isolates indicate that consuming goose meat might cause a potential risk, therefore leading to human campylobacteriosis.

Detection of virulence genes determining the ability to adhere and invade in Campylobacter spp. from cattle and swine in Poland.

The aim of the study was to determine the prevalence of virulence genes responsible for the adhesion (flaA, cadF and racR) and invasion (virB11, iam and pldA) in Campylobacter isolates from cattle and swine and determine their adherence and invasion abilities. The studies conducted revealed high prevalence rate of adherence and invasion associated genes irrespective of the isolates origin. All Campylobacter strains of swine and cattle origin adhered to HeLa cells at mean level 0.1099% ±â€¯SD 0.1341% and 0.0845% ±â€¯SD 0.1304% of starting viable inoculum, respectively. However swine isolates exhibited higher invasion abilities (0.0012% ±â€¯SD 0.0011%) compared to bovine isolates (0.00038% ±â€¯SD 0.00055%). The results obtained revealed significantly positive correlation between invasion and adherence abilities of swine origin isolates (R = 0.4867 in regard to C. jejuni and R = 0.4507 in regard to C. coli) and bovine origin isolates (R = 0.726 in regard to C. jejuni). Bacterial virulence is multifactorial and it is affected by the expression of virulence genes. Moreover the presence of virulence genes determines the ability of Campylobacter isolates to adhere and invade the cells.