Silver nanoparticles promote osteogenic differentiation of mouse embryonic fibroblasts in vitro.

OBJECTIVE: This study investigated if silver nanoparticles (AgNps) could promote the proliferation and osteogenic differentiation of mouse embryonic fibroblasts. METHODS: Mouse embryonic fibroblasts were divided into two groups: Group 1 cells were cultured in DMEM/F12 medium and Group 2 cells were cultured in osteogenic medium. Both groups were then treated with 16, 32, or 100 µM AgNps. Fibroblast proliferation and viability were measured using BrdU and MTT methods at varying time points. Alizarin red staining and alkaline phosphatase (ALP) activity were measured to observe fibroblast differentiation into osteoblasts. Proteomics (cytokine array) was used to detect 111 different cytokines during differentiation. RESULTS: AgNps stimulated proliferation of mouse embryonic fibroblasts at a concentration of 16 µM. Marked enhancement of calcium mineralization was observed in cells cultured with AgNps compared with cells cultured without AgNps. Group 2 cells displayed nodules around the center where the cell density was high. ALP activity of mouse embryonic fibroblasts cultured in osteogenic medium increased during the whole culture period. Addition of AgNps at concentrations of 32 µM and 100 µM induced higher ALP activity at days 7 and 14. Proteomic array results show that low density lipoprotein receptor (LDL-R) and proprotein convertase subtilisin/kexin type 9 (PCSK-9) were significantly increased, while osteoprotegerin (OPG) was significantly reduced in medium containing 16 µM AgNPs. CONCLUSION: AgNps could promote differentiation of mouse embryonic fibroblasts into osteoblastic cells. LDL-R and PCSK-9, as well as OPG, may play a critical role in this process.

Direct cellular reprogramming and transdifferentiation of fibroblasts on wound healing-Fantasy or reality?

Induced pluripotent stem cell (iPSC) technology is one of the de novo approaches in regeneration medicine and has led to new research applications for wound healing in recent years. Fibroblasts have attracted wide attention as the first cell line used for differentiation into iPSCs. Researchers have found that fibroblasts can be induced into different types of cells in variable mediums or microenvironments. This indicates the potential "stem" characteristics of fibroblasts in terms of direct cellular reprogramming compared with the iPSC detour. In this review, we described the morphology and biological function of fibroblasts. The stem cell characteristics and activities of fibroblasts, including transdifferentiation into myofibroblasts, osteogenic cells, chondrogenic cells, neurons, and vascular tissue, are discussed. The biological values of fibroblasts are then briefly reviewed. Finally, we discussed the potential applications of fibroblasts in clinical practice.

Detecting gender as a moderator in meta-analysis: The problem of restricted between-study variance.

Meta-analyses in the psychological sciences typically examine moderators that may explain heterogeneity in effect sizes. One of the most commonly examined moderators is gender. Overall, tests of gender as a moderator are rarely significant, which may be because effects rarely differ substantially between men and women. While this may be true in some cases, we also suggest that the lack of significant findings may be attributable to the way in which gender is examined as a meta-analytic moderator, such that detecting moderating effects is very unlikely even when such effects are substantial in magnitude. More specifically, we suggest that lack of between-primary study variance in gender composition makes it exceedingly difficult to detect moderation. That is, because primary studies tend to have similar male-to-female ratios, there is very little variance in gender composition between primaries, making it nearly impossible to detect between-study differences in the relationship of interest as a function of gender. In the present article, we report results from two studies: (a) a meta-meta-analysis in which we demonstrate the magnitude of this problem by computing the between-study variance in gender composition across 286 meta-analytic moderation tests from 50 meta-analyses, and (b) a Monte Carlo simulation study in which we show that this lack of variance results in near-zero moderator effects even when male-female differences in correlations are quite large. Our simulations are also used to show the value of single-gender studies for detecting moderating effects. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Task-related and task-unrelated thoughts in runners and equestrians: Measurement issues in evaluations of thought content.

Much of the thought content and mind-wandering literature examines self-reported thought content's relationship with performance criteria in limited ways. Furthermore, retrospective reports about thought content may be influenced by the quality of one's performance. We explored these method issues in a cross-sectional study of individuals competing in a trail race and an equestrian event. Our results demonstrated that self-reports of thought content differed based on the performance context: whereas runners' task-related and task-unrelated thoughts were negatively correlated, equestrians' thought content showed no relationship. Moreover, equestrians in general reported fewer task-related and task-unrelated thoughts than runners. Finally, objective performance predicted task-unrelated thought (but not task-related thought) among runners, and an exploratory mediation test suggested the effect was partially mediated by performance awareness. We discuss the applied implications of this research for human performance practitioners.

Assessing the Impact of Community Factors on Acute Care Nurses' Job Satisfaction and Turnover Intention.

BACKGROUND: Community factors may affect nurses' job behavior and decision making. There is a gap in the literature regarding the impact of community satisfaction, family ties, and community preferences on acute care nurses' turnover intention and job satisfaction. Furthermore, no studies have examined the differences in community satisfaction, community preferences, and family ties among nurses working in rural and urban settings. PURPOSE: To identify the impact of family ties, community satisfaction, and community preferences on turnover intention and job satisfaction among acute care nurses working in Ontario's urban and rural areas. METHODS: Descriptive correlational survey design was used in this study. A targeted stratified sampling technique was used to recruit acute care nurses working in Ontario's urban and rural areas (N = 349) between May 2019 and July 2019. Dillman's approach was used to guide data collection. Parametric and non-parametric tests were used for data analysis. RESULTS: A significant association was found between working settings and community preferences. A statistically significant positive relationship between community satisfaction and nurses' job satisfaction was identified. Furthermore, community satisfaction had a negative impact on turnover intention. Neither community preference nor family ties were significantly associated with turnover intention or job satisfaction. CONCLUSION: The study suggests that community satisfaction can influence important nurse work-related outcomes. Future studies should replicate and validate these results in different contexts and cultures. Retaining nurses may be difficult if they are not satisfied with their communities.

The influence of authentic leadership on nurses' turnover intentions and satisfaction with quality of care in Saudi Arabia: A structural equation modelling study.

AIM(S): The study aimed to test a model that examined the relationships between authentic leadership, psychological safety, work engagement and team effectiveness and subsequent effects of team effectiveness on job turnover intentions and nurse satisfaction with quality of care. BACKGROUND: Nurse leaders who exhibit authentic leadership have been shown to contribute to the development of healthy work environments. In workplaces with demonstrated authentic leadership, nurses are more engaged and have lower job turnover intentions. METHOD(S): A non-experimental, cross-sectional design was used to test the hypothesized model via structural equation modelling. A total of 456 nurses were included. RESULTS: Structural equation modelling analysis indicated a good fit for the hypothesized model. Authentic leadership had a positive, significant and direct relationship with team effectiveness, nurses' work engagement and psychological safety. Team effectiveness was found to be negatively related to job turnover intentions but positively related to nurse satisfaction with quality of care. CONCLUSION(S): Results of this study may help nursing leaders have a better understanding of the essential role of leadership style in increasing healthy work environments. IMPLICATIONS FOR NURSING MANAGEMENT: Applying authentic leadership style in nursing practice could help to reduce the shortage of nurses stemming from the high turnover intentions of nurses.

Structural and construct validity of the Quality of Life Scale among Canadian women with histories of intimate partner violence.

OBJECTIVES: To evaluate the structural and construct validity, and internal consistency of the Quality of Life Scale among Canadian women with histories of intimate partner violence. METHODS: Consistent with COSMIN Guidelines, a secondary analysis was conducted using data from a community sample of 250 adult (over 18 years) Canadian women with histories of partner violence and who participated in Wave 5 of the longitudinal Women's Health Effects Study. Data were collected 4 years after baseline using structured interviews that included the Quality of Life Scale and two mental symptom scales (Center for Epidemiological Depression Scale and Davidson Trauma Scale) used to assess construct validity of the Quality of Life Scale. RESULTS: Confirmatory factor analysis in MPLUS 8 with maximum likelihood estimation supported the hypothesized unidimensional structure of the 9-item Quality of Life Scale based on acceptable fit indices. Internal consistency, estimated using Cronbach's alpha and composite reliability, were .91 and .92, respectively, with item-total correlations ranging from .46 to .84. Inter-item correlation coefficients (range = .30-.79), suggesting that all items contribute to the total score. As hypothesized, the quality of life total score was negatively related to the total scores on both the Center for Epidemiologic Studies-Depression (r = -.739) and Davidson Trauma Scale (r = -.537), providing evidence of construct validity of the Quality of Life Scale. CONCLUSION: The Quality of Life Scale is a brief, reliable, valid, unidimensional self-report measure appropriate for use with women who have experienced partner violence. By addressing an important measurement gap, results of this study have potential to advance research on women's quality of life in the context of partner violence, including improving the evaluation of a growing body of advocacy and health interventions designed to support women's healing and well-being.

A Psychometric Analysis of the Nurse Satisfaction with the Quality of Care Scale.

The concept of quality of nursing care can vary across healthcare organizations, and many different factors may affect the quality of nursing care as perceived by nurses. Measuring satisfaction with quality of nursing care from the nurse's perspective is important as a valid and reliable indicator of care quality. The purpose of this study was to measure the psychometric properties of a researcher-developed instrument measuring nurse satisfaction with quality of care. A sample of 200 nurses was randomly selected from three different cities in Saudi Arabia and surveyed with the Nurse Satisfaction with Quality of Care Scale, which is a self-administrated five-item scale. Exploratory factor analysis, confirmatory factor analysis, and internal consistency analysis were conducted to assess aspects of the validity and reliability of the instrument. The results of exploratory factor analysis supported a one-factor structure that consisted of the five items. Confirmatory factor analysis results confirmed that the five items were integral to nurse satisfaction with quality of care. The Cronbach internal consistency of the scale was acceptable. The scale appeared to be a reliable and valid tool for assessing nurse perceptions of their satisfaction with the quality of care provided. Additional studies to further test psychometric properties of this scale in different contexts are warranted.

An integrative review of nursing leadership in Saudi Arabia.

AIMS: The aims of this integrative review were to describe leadership styles from the nursing literature in Saudi Arabia and to identify the current state of evidence about relationships between leadership styles and nurse, patient and organization outcomes in Saudi Arabia. DESIGN: Integrative review was used as a design for this study. METHODS: The following search terms were used with databases: 'Saudi Arabia', 'leadership theory*', 'leadership style*', 'leadership model*', 'management style' and 'nurse*'. Methodological quality was assessed using two different quality rating tools for quantitative and qualitative studies. Databases used for this review included Nursing & Allied Health Database, Cochrane Database of Systematic Reviews, PubMed, CINAHL, Embase, PsycINFO, Scopus, Web of Science and ProQuest Dissertations & Theses. RESULTS: Nine manuscripts representing eight studies were included in this review. The papers reviewed included quantitative (n = 6), qualitative (n = 2) and mixed methods studies (n = 1). Results were grouped into different themes, identified as nursing leadership styles in Saudi Arabia, leadership styles and nurses' outcomes, and demographics and leadership styles.

Meconium peritonitis: A 22-year review in a tertiary referral center.

BACKGROUND: Meconium peritonitis (MP) is a sterile, chemical peritonitis resulting from in-utero fetal bowel perforation. Severe cases may lead to serious morbidities and mortalities. OBJECTIVE: To review the common antenatal ultrasound abnormalities associated with MP, and identify radiological and clinical prognostic factors. MATERIALS AND METHODS: Retrospective review of all neonates with MP from January 1997 to December 2019 treated in our hospital was performed. Antenatal ultrasound findings, clinical presentations and outcomes were analyzed. RESULTS: Thirty-five neonates (17 males, 18 females) were included in the study. Thirty-two (91.4%) attended antenatal screening, and 27 (84.4%) of them had abnormalities identified on antenatal ultrasound. The most common abnormality was polyhydramnios (43.8%). Nineteen (54.3%) patients were inborn. Twenty (57.1%) patients were born prematurely. Laparotomy was required in 85.7% of patients. The median time to laparotomy was shorter in the inborn group [1 day (0-9 days) vs 4 days (2-34 days), p = 0.001], but the duration of post-operative hospital stay was comparable [71 days (16-423 days) vs 73.5 days (23-231 days)]. However, such duration was found to be significantly longer in the pre-term group when compared to full-term [58.5 days (16-89 days) vs 85 (21-423 days), p = 0.01]. The most common pathology was small bowel atresia and there were two mortalities. CONCLUSION: Due to the advancement in prenatal detection, pediatric anesthesia, intensive care and surgical techniques, the morbidity and mortality of MP has much decreased. Effective multi-disciplinary antenatal counseling facilitated the perinatal management of MP and resulted in comparable prognosis and outcome in inborn and outborn neonates.

Gastrostomy tube migration causing gastric outlet obstruction and gastric perforation in children-two case reports.

Gastrostomy creation is a common procedure in paediatric surgery. It provides enteral nutrition to patients who cannot achieve adequate nutrition by mouth. Common indications of gastrostomy placement in children include syndromic or neurodevelopmental diseases, severe malformations of the aerodigestive tract, craniofacial abnormalities, and intractable gastroesophageal reflux disease with recurrent aspiration. It is also indicated in patients who suffer from failure to thrive and require extra caloric intake. In contrast to adults, paediatric patients have a weaker ability to complain about problems with their gastrostomy tubes when they occur, in particular when some of these neurological patients are non-communicable. Complications may arise when migration of the catheter goes unnoticed, causing obstruction. Due to the smaller stomach size, thinner gastric wall and narrower gastric outlet in paediatric patients, obstruction from the gastrostomy balloon may result in serious sequelae. Here we present two unusual but serious complications of gastrostomy tubes causing suspected duodenal obstruction and subsequent gastric perforation in paediatric patients, requiring emergency operations. Furthermore, we would like to emphasize the importance of proper gastrostomy tube care and alert the caretakers of the potential sinister complications that can arise. We would also like to take this opportunity to suggest measures to prevent their occurrence.

Development and Testing of an Acute Care Nurses' Job Satisfaction Scale.

BACKGROUND AND PURPOSE: Factors affecting nurses' job satisfaction in the acute care setting may differ from nurses working in other settings. The aim of this study was to develop a new tool that measure the job satisfaction of acute care nurses who provide direct patient care. METHODS: Content validity then exploratory factor analysis (EFA) were used for validation of the new tool using a random sample of 349 acute care nurses. RESULTS: The EFA resulted in 31 items suggesting 6 factors were present in the instrument. The identified factors were: achievement/job interest/responsibility, hospital policy, quality of supervision, peer support/work condition, growth/advancement, and benefits/job security. CONCLUSION: A new tool for measuring acute care nurses' job satisfaction was developed based on a robust theoretical foundation and demonstrated sound psychometric properties.

Relationships among sex, empowerment, workplace bullying and job turnover intention of new graduate nurses.

AIMS AND OBJECTIVES: The purpose of this study was twofold: examine the relationships among new graduate nurses' (NGNs) structural empowerment, experience of workplace bullying, and their job turnover intention and assess the relationships between sex, workplace bullying, and job turnover intention. BACKGROUND: Nursing research has highlighted the issue of workplace bullying and its negative impacts. Despite increased awareness, male nurses and their responses to bullying have not been a significant focus of study. DESIGN: A secondary analysis of data collected from a random sample of 1008 Canadian NGNs from the following: Starting Out: A time-lagged Study of New Graduate Nurses' Transition to Practice. METHODS: All data were analysed using SPSS, and the study model was tested using the SPSS PROCESS macro, specifically Model 4 (for simple mediation). This paper is compliant with the STROBE reporting guideline for cross-sectional studies. RESULTS: Structural empowerment significantly predicted workplace bullying and job turnover intention. Workplace bullying significantly predicted job turnover intention. Structural empowerment mediated job turnover intention through workplace bullying. Male new graduate nurses reported significantly higher workplace bullying than female NGNs yet lower job turnover intention. CONCLUSIONS: The findings demonstrate the positive effects of structural empowerment on both decreasing workplace bullying and job turnover intention. Furthermore, findings showed the influence of sex on workplace bullying and job turnover intention. The findings contribute to literature on male NGNs and suggest that they experience significantly higher rates of workplace bullying than their female counterparts. The findings suggest differences exist in the workplace experience for male and female NGNs that future research may help reveal. RELEVANCE TO CLINICAL PRACTICE: The findings suggest structural empowerment may be used to reduce bullying prevalence and reduce job turnover intention consequently. The findings also suggest that some measures are needed to address the higher frequency of bullying experienced by male NGNs.

"Worse than I anticipated" or "This isn't so bad"?: The impact of affective forecasting accuracy on self-reported task performance.

Various motivational theories emphasize that desired emotional outcomes guide behavioral choices. Although motivational theory and research has emphasized that behavior is affected by desired emotional outcomes, little research has focused on the impact of anticipated feelings about engaging in behavior. The current research seeks to partly fill that void. Specifically, we borrow from affective forecasting research in suggesting that forecasts about engaging in performance-relevant behaviors can be more or less accurate. Furthermore, we suggest that the degree of accuracy has implications for self-reported task performance. To examine these ideas, we conducted two studies in which individuals made affective predictions about engaging in tasks and then later reported how they actually felt during task engagement. We also assessed their self-reported task performance. In Study 1, 214 workers provided affective forecasts about upcoming work tasks, and in Study 2, 185 students made forecasts about studying for an exam. Results based on polynomial regression were largely consistent across the studies. The accuracy of the forecasts did not conform to the pattern of affective forecasting accuracy typically found outside the performance domain. Furthermore, anticipated and experienced affect jointly predicted self-reported task performance in a consistent manner. Collectively, these findings suggest that taking into account anticipated affect, and its relationship with later experienced affect, provides a more comprehensive account of affect's role in task performance.

Factors affecting job satisfaction among acute care nurses working in rural and urban settings.

AIMS: To: (a) identify the differences and similarities in the extrinsic and intrinsic factors that influence job satisfaction among nurses in urban and rural Ontario; and (b) determine the impact of job satisfaction on nurses' turnover intention among nurses working in rural and urban settings in Ontario. DESIGN: Cross-sectional correlational design was used for this study. METHODS: Data were collected between May 2019-July 2019 in southern Ontario. Participants (N=349) completed the Acute Care Nurses' Job Satisfaction Scale and The Anticipated Turnover Scale. A stratified sampling technique was used for recruiting the sample population and participants were given the option to respond either online or by mailed survey. RESULTS: There was no significant difference between rural and urban nurses in either overall job satisfaction level or turnover intention. Peer support/work conditions, quality of supervision, and achievement/job interest/responsibility were significant predictors of job satisfaction. There was a significant difference between rural and urban nurses in terms of satisfaction from benefits and job security and the nurses' job satisfaction levels correlated negatively with their turnover intention. CONCLUSION: Several extrinsic and intrinsic factors are associated with nurses' job satisfaction in rural and urban settings. Developing strategies that improve satisfaction by modulating these specific factors may improve nurses' job satisfaction and reduce turnover. IMPACT: This study discussed how working in a rural or urban hospital may affect nurses' job satisfaction and turnover intention. The findings can help in improving nurses' job satisfaction and inform workforce planning to increase nurses' retention.

Real-world gaseous and particulate emissions from Euro IV to VI medium duty diesel trucks.

This study offers emission factors for earlier and late technology medium duty diesel particulate filter (DPF) -equipped trucks, operating on real-world conditions. The analysis includes levels of nitrous oxide (N2O) emissions as well as the impacts of DPF regenerations on emission levels. The real-driving gaseous and particulate emissions, as well as fuel consumption of 14 Euro IV, Euro V, and Euro VI medium duty diesel trucks were analysed and the efficiency of different emission control technologies were assessed. Measurements were conducted using portable emission measurement systems (PEMS) over a wide range of driving and operating conditions. Distance-based integration of emission rates over 500 m sections was considered for statistical analysis, providing a large dataset of emission factors to be used for network link-based traffic and emissions modelling. In terms of emissions performance, nitrogen oxides (NOx) levels were in general above the corresponding Euro standard limits, while carbon monoxide (CO), total hydrocarbons (THC) and particulate matter (PM) levels were within limits. Selective catalytic reduction (SCR) -equipped Euro V vehicles were seen to emit more than their non-SCR-equipped counterparts. NOx and fuel consumption were positively correlated with road gradient over the -6% to 6% gradient range. The emission levels of ammonia (NH3) were measured significantly lower than the respective Euro VI provisions for heavy duty engines, while the N2O levels were found to contribute approximately 1% to the respective total greenhouse gases levels. DPF regeneration events in real world seem to have a measurable impact mostly on THC and PM emissions, increasing baseline levels by 8.1% and 29%, respectively, for Euro VI vehicles.

Leader empowering behaviour: relationships with nurse and patient outcomes.

PURPOSE: This study aims to test a model examining the impact of leader empowering behaviour on experienced nurses' self-efficacy, interprofessional collaboration, job turnover intentions and adverse patient outcomes. DESIGN/METHODOLOGY/APPROACH: Structural equation modelling in Mplus was used to analyse cross-sectional survey data from experienced nurses in Alberta, Ontario, and Nova Scotia, Canada (n = 478). FINDINGS: The results supported the hypothesized model: (164) = 333.021, p = 0.000; RMSEA = 0.047; CFI = 0.965; TLI = 0.959; SRMR = 0.051. Indirect effects were observed between leader empowering behaviour and nurses' assessment of adverse events and leader empowering behaviour and nurses' job turnover intentions through interprofessional collaboration. RESEARCH LIMITATIONS/IMPLICATIONS: Leader empowering behaviour plays a role in creating collaborative conditions that support quality patient care and the retention of experienced nurses. PRACTICAL IMPLICATIONS: The findings will be of interest to academic and hospital leaders as they consider strategies to retain experienced nurses, such as nurse manager selection, development and performance management systems. ORIGINALITY/VALUE: The influx of new graduate nurses to the nursing profession and changing models of care requires the retention of experienced nurses in the workforce. The findings suggest that leader empowering behaviour and interprofessional collaboration are important factors in supporting quality patient care and stabilizing the nursing workforce.

Factors affecting nurses' job satisfaction in rural and urban acute care settings: A PRISMA systematic review.

AIMS: This review aimed to systematically assess the findings of primary studies to identify the factors associated with nurse job satisfaction in rural and urban contexts while analysing the findings according to Herzberg's theory. BACKGROUND: While there is evidence linking several factors to nurses' job satisfaction, gaps still exist in understanding the differences between factors affecting job satisfaction for nurses working in rural and urban settings. DESIGN: Systematic review with narrative summary. DATA SOURCES: Six databases were used to identify original studies that discuss the factors associated with the nurse's job satisfaction between 1998-2018. REVIEW METHODS: Two authors independently reviewed each study using the Joanna Briggs Institute's critical appraisal checklists. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement to report and examine the study biases systematically. RESULTS: A total of 38 studies were selected for this review. Physical working environment and authority and freedom were the most frequently reported factors associated with nurses' job satisfaction. Several extrinsic, intrinsic, personal, and community factors were also found to be associated with nurses' job satisfaction. Urban studies tended to focus on extrinsic factors, whereas there was more balance between the two sets of factors in rural studies. CONCLUSION: Both intrinsic and extrinsic factors play an essential role in nurses' job satisfaction. Future research should use more robust research methods and pay more attention to contrasting rural and urban contexts. Herzberg's theory can provide conceptual clarity when investigating the factors associated with nurses' job satisfaction. IMPACT: This review discussed the factors associated with nurses' job satisfaction in rural and urban settings. The findings linked several extrinsic and intrinsic factors to nurses' job satisfaction. Nursing management should search for the perfect blend of intrinsic and extrinsic factors based on nurses' needs and organizational commitment to improve nurses' job satisfaction.