RESUMEN
The effects of biochanin A on the growth and differentiation of a recently characterized myeloid leukemia cell line WEHI-3B (JCS) were investigated. Biochanin A not only inhibited the growth of JCS cells in a dose-dependent manner (0 - 200 microM) but also induced the morphological differentiation of JCS cells. The phagocytic activity of biochanin A-treated JCS cells was also increased. Flow cytometric analysis showed that the expression of macrophage differentiation markers Mac-1 and F4/80 was up-regulated in biochanin A-treated JCS cells. The expression level of Mac-1 was higher than that of F4/80. The expression of cytokine genes was studied by reverse transcription-polymerase chain reaction (RT-PCR) and cycle titration. mRNA levels of IL-1alpha, IL-1beta and IL-4 were found to be up-regulated at 46 hours after incubation of JCS cells with biochanin A. Although the expression of LIF was also up-regulated, the LIF receptor gene was not expressed in the uninduced or induced JCS cells. Our results suggest that IL-1alpha, IL-1beta and IL-4 may act on the later stage of biochanin A-mediated differentiation of JCS cells.
Asunto(s)
Diferenciación Celular/efectos de los fármacos , División Celular/efectos de los fármacos , Genisteína , Interleucina-6 , Isoflavonas/farmacología , Leucemia Mieloide/patología , Macrófagos/citología , Animales , Expresión Génica , Inhibidores de Crecimiento/genética , Inhibidores de Crecimiento/fisiología , Interleucina-1/genética , Interleucina-1/fisiología , Interleucina-4/genética , Interleucina-4/fisiología , Factor Inhibidor de Leucemia , Leucemia Mieloide/genética , Linfocinas/genética , Linfocinas/fisiología , Macrófagos/efectos de los fármacos , Ratones , Fenotipo , ARN Mensajero/genética , Células Tumorales Cultivadas , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
In vitro effects of medicinal plant extracts from the pericarpium of Citrus reticulata (cv Jiao Gan) (PCRJ) on the growth and differentiation of a recently characterized murine myeloid leukemic cell clone WEHI 3B (JCS) were investigated. Extracts of PCRJ not only inhibited the proliferation of JCS cells in a dose dependent manner, but also induced differentiation of JCS cells into macrophages and granulocytes. Morphological differentiation of PCRJ treated JCS cells was associated with an increase in phagocytic activity of the cells. Furthermore, both in vitro clonogenicity and in vivo growth of PCRJ treated JCS leukemic cells in syngeneic BALB/c mice were significantly reduced. The survival rate of mice receiving PCRJ treated JCS tumour cells was also increased. Using 1H-NMR, 13C-NMR, and GC/MS, two active components isolated from PCRJ were identified as nobiletin and tangeretin.
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Citrus/química , Leucemia Mieloide/tratamiento farmacológico , Extractos Vegetales/aislamiento & purificación , Animales , Diferenciación Celular/efectos de los fármacos , División Celular/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos BALB C , Extractos Vegetales/farmacología , Células Tumorales CultivadasRESUMEN
Extracts of both sugarcane and soybean wastes supported the growth of Monascus but sugarcane waste was superior for the production of α-galactosidase. An aqueous extract prepared from 5% (w/v) soybean waste and 7% (w/v) sugarcane waste gave the best result and was superior to the standard peptone/glucose/yeast extract medium. Liquid-solid mixtures were slightly less effective. Enzyme production could be enhanced by adding raffinose. Enzymatic hydrolysis of p-nitrophenyl-α-D-galactoside was optimal at pH 4.5. Raffinose and stachyose were hydrolysed to sucrose and galactose.
