RESUMEN
Intervertebral disc degeneration is the main cause of lumbago disease, in which the extracellular matrix structure and moisture in the nucleus pulposus is lost continuously. In this study, we aimed to detect differential expression of silence mating type information regulation 2 homolog 1 (SIRT1) and matrix metalloproteinase-1 (MMP-1) in human intervertebral disc nucleus pulposus cells and to explore the effects of SIRT1 and MMP-1 on the development of the intervertebral disc degeneration. Intervertebral disc nucleus pulposus specimens from 41 patients who underwent lumbar protrusion resection at HuiZhou Municipal Central Hospital, during the period from October 2011 to December 2013, were studied in comparison with 23 control cases from patients who underwent fractured lumbar resection. In degenerated human intervertebral disc nucleus pulposus cells, the expression of SIRT1 is decreased and MMP-1 is increased compared with that of the control cells. Resveratrol could reverse these effects, thereby increasing the expression of SIRT1 (0.87 ± 0.07 vs 0.54 ± 0.04), Coll2α1 (0.90 ± 0.08 vs 0.38 ± 0.01), and aggrecan (0.69 ± 0.07 vs 0.42 ± 0.05) and decreasing the expression of MMP-1 (0.61 ± 0.03 vs 0.93 ± 0.08). These results suggest that resveratrol could possibly reverse the process of intervertebral disc degeneration and thus could be applied as a potential drug for the disease.
Asunto(s)
Regulación de la Expresión Génica/efectos de los fármacos , Metaloproteinasa 1 de la Matriz/genética , Sirtuina 1/genética , Estilbenos/farmacología , Adulto , Anciano , Agrecanos/metabolismo , Células Cultivadas , Colágeno Tipo II/metabolismo , Femenino , Humanos , Inmunohistoquímica , Técnicas In Vitro , Masculino , Metaloproteinasa 1 de la Matriz/metabolismo , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Resveratrol , Sirtuina 1/metabolismo , Adulto JovenRESUMEN
To identify genes of potential importance to alkalinity tolerance, RNA sequencing (RNA-Seq) was performed to survey gill transcriptome profiles from freshwater (FW) and alkaline water (AW) exposed Nile tilapia (Oreochromis niloticus). A total of 22,724,036 (AW)/16,461,040 (FW) single-end reads were generated in which 20,304,348 (AW)/14,681,290 (FW) reads (90.0/89.72%) were aligned to the reference genome. Differential expression analysis revealed 302 up-regulated and 193 down-regulated genes between AW- and FW-exposed fish. These differentially expressed genes were enriched in several Gene Ontology (GO) terms related to "stress response", "heme binding", and "carbonate dehydratase activity". Meanwhile, significant KEGG pathways were enriched in energy metabolism, including nitrogen and sulfur metabolism. These results demonstrate the response of Nile tilapia exposed to alkaline-water and might provide valuable information to further understand the molecular mechanisms of adaptation of fish to alkaline environments.
Asunto(s)
Perfilación de la Expresión Génica , Regulación de la Expresión Génica/efectos de los fármacos , Tilapia/genética , Transcriptoma/genética , Adaptación Fisiológica/efectos de los fármacos , Adaptación Fisiológica/genética , Álcalis/toxicidad , Animales , Branquias/efectos de los fármacos , Branquias/metabolismo , Estrés Fisiológico/efectos de los fármacos , Transcriptoma/efectos de los fármacosRESUMEN
Paulownia kawakamii is a fast-growing timber tree. In this study, 21 primer sets were developed using an enriched genomic library. The genetic diversity was measured in one P. kawakamii population. The number of alleles per locus ranged from 2 to 19. The observed and expected heterozygosities varied from 0.158 to 0.842 (mean = 0.421) and from 0.376 to 0.952 (mean = 0.771), respectively. All 21 loci were also polymorphic in closely related species (P. tomentosa, P. elongata, and P. fortunei). The described markers will be useful in future population genetic studies and molecular breeding of these Paulownia species.