Microstructures, Mechanical Properties and Electromagnetic Wave Absorption Performance of Porous SiC Ceramics by Direct Foaming Combined with Direct-Ink-Writing-Based 3D Printing.

Direct-ink-writing (DIW)-based 3D-printing technology combined with the direct-foaming method provides a new strategy for the fabrication of porous materials. We herein report a novel method of preparing porous SiC ceramics using the DIW process and investigate their mechanical and wave absorption properties. We investigated the effects of nozzle diameter on the macroscopic shape and microstructure of the DIW SiC green bodies. Subsequently, the influences of the sintering temperature on the mechanical properties and electromagnetic (EM) wave absorption performance of the final porous SiC-sintered ceramics were also studied. The results showed that the nozzle diameter played an important role in maintaining the structure of the SiC green part. The printed products contained large amounts of closed pores with diameters of approximately 100-200 µm. As the sintering temperature increased, the porosity of porous SiC-sintered ceramics decreased while the compressive strength increased. The maximum open porosity and compressive strength were 65.4% and 7.9 MPa, respectively. The minimum reflection loss (RL) was -48.9 dB, and the maximum effective absorption bandwidth (EAB) value was 3.7 GHz. Notably, porous SiC ceramics after sintering at 1650 °C could meet the application requirements with a compressive strength of 7.9 MPa, a minimum RL of -27.1 dB, and an EAB value of 3.4 GHz. This study demonstrated the potential of direct foaming combined with DIW-based 3D printing to prepare porous SiC ceramics for high strength and excellent EM wave absorption applications.

Additive Manufacturing of Bioceramic Implants for Restoration Bone Engineering: Technologies, Advances, and Future Perspectives.

Treating bone defects is highly challenging because they do not heal on their own inside the patients, so implants are needed to assist in the reconstruction of the bone. Bioceramic implants based on additive manufacturing (AM) are currently emerging as promising treatment options for restoration bone engineering. On the one hand, additively manufactured bioceramic implants have excellent mechanical properties and biocompatibility, which are suitable for bone regeneration. On the other hand, the designable structure and adjustable pores of additively manufactured bioceramic implants allow them to promote suitable cell growth and tissue climbing. Herein, this review unfolds to introduce several frequently employed AM technologies for bioceramic implants. After that, advances in commonly used additively manufactured bioceramic implants, including bioinert ceramic implants, bioactive ceramic implants, and bioceramic/organic composite implants, are categorized and summarized. Finally, the future perspectives of additively manufactured bioceramic implants, in terms of mechanical performance improvement, innovative structural design, biological property enhancement, and other functionalization approaches, are proposed and forecasted. This review is believed to provide some fundamental understanding and cutting-edge knowledge for the additive manufacturing of bioceramic implants for restoration bone engineering.

Proteomic analysis of skeletal muscle in Chinese hamsters with type 2 diabetes mellitus reveals that OPLAH downregulation affects insulin resistance and impaired glucose uptake.

Type 2 diabetes mellitus (T2DM) is a metabolic disease controlled by a combination of genetic and environmental factors. The Chinese hamster, as a novel animal model of spontaneous T2DM with high phenotypic similarity to human disease, is of great value in identifying potential therapeutic targets for T2DM. Here, we used tandem mass tag (TMT) quantitative proteomics based on liquid chromatography-tandem mass spectrometry to assess the skeletal muscles of a Chinese hamster diabetes model. We identified 38 differentially abundant proteins, of which 14 were upregulated and 24 were downregulated. Further analysis of the differentially abundant proteins revealed that five of them (OPLAH, GST, EPHX1, SIRT5, ALDH1L1) were associated with oxidative stress; these were validated at the protein and mRNA levels, and the results were consistent with the proteomic analysis results. In addition, we evaluated the role of OPLAH in the pathogenesis of T2DM in human skeletal muscle cells (HSKMCs) by silencing it. The knockdown of OPLAH caused an increase in reactive oxygen species content, decreased the GSH content, inhibited the PI3K/Akt/GLUT4 signaling pathway, and reduced glucose uptake. We propose that OPLAH downregulation plays a role in insulin resistance and glucose uptake disorders in HSKMCs possibly via oxidative stress, making it a new therapeutic target for T2DM.

An Innovative Auxetic Honeycomb Sandwich Tube: Fabrication and Mechanical Properties.

In this study, based on the free-rolling mechanism of the auxetic honeycomb, a honeycomb cylindrical shell was successfully prepared to overcome the fracture problem of the hexagonal honeycomb during rolling. Auxetic honeycomb sandwich tubes (AHSTs) with a variable Poisson's ratio were fabricated by molding and bonding. A Poisson's ratio model of the auxetic honeycomb core was developed based on the strain increment ratio of the deformed honeycomb and validated using computed tomography (CT). Four failure modes (progressive stable fold mode I, unstable local buckling mode II, transverse shearing mode III, and mid-length collapse mode IV) of the AHST were summarized by comparing the deformation behavior and force-displacement curves with different geometric parameters. When the aspect ratio R is greater than 3, the AHST will be more easily damaged in instability (Mode IV). Static compression tests showed that the peak force (PF) and crushing force efficiency (CFE) of the AHST were higher than those of the CFRP thin-walled tube of the same diameter by 78% and 115%, respectively. Therefore, the AHST has excellent mechanical properties and it is feasible to use the auxetic honeycomb as a core for sandwich structures.

Dynamic Responses of Blast-Loaded Shallow Buried Concrete Arches Strengthened with BFRP Bars.

This paper proposes a prefabricated basalt fiber reinforced polymer (BFRP) bars reinforcement of a concrete arch structure with superior performance in the field of protection engineering. To study the anti-blast performance of the shallow-buried BFRP bars concrete arch (BBCA), a multi-parameter comparative analysis was conducted employing the LS-DYNA numerical method, which was verified by the results of the field explosion experiments. By analyzing the pressure, displacement, acceleration of the arch, and the strain of the BFRP bars, the dynamic response of the arch was obtained. This study showed that BFRP bars could significantly optimize the dynamic responses of blast-loaded concrete arches. The damage of exploded BBCA was divided into five levels: no damage, slight damage, obvious damage, severe damage, and collapse. BFRP bars could effectively mitigate the degree of damage of shallow-buried underground protective arch structures under the explosive loads. According to the research results, it was feasible for BFRP bars to be used in the construction of shallow buried concrete protective arch structures, especially in the coastal environments.

Responses of microbial community to different concentration of perchlorate in the Qingyi River.

Perchlorate is a refractory and mobile contaminant that is wildly distributed in surface water, and due to its tremendous inhibitory effect on mammalian thyroid function, it has gained much attention in recent years. Numerous studies have focused on environmental detection of perchlorate, especially in water. However, less attention has been paid to the effects of perchlorate on the composition of the microbial community in rivers. Upstream of the Qingyi River, an important source of drinking water for local residents, there are two perchlorate manufacturers. In this study, we selected eight study sites from upstream to downstream of the Qingyi River, including sites located upstream and downstream of the perchlorate manufacturers. Our results indicated that perchlorate was detected in all sites except for QYR2, QYR3, and QYR10. The concentration of perchlorate in the five study sites was much higher than the reference dose proposed by the National Academy of Science, and ranged from 187 to 9647.00 µg/L. We utilized 16S rDNA high throughput sequencing to analyze changes in the composition of the microbial community, based on the Illumina 2 × 250 MiSeq platform. The results showed that, when microbial communities were exposed to high concentration of perchlorate, there was an increase in the ratio of Betaproteobacteria, Bacteroidetes, Actinobacteria, and Saccharibacteria in the microbial community along with a decrease in the ratio of Chloroflexi, Verrucomicrobia, and Gammaproteobacteria. Our study has provided a theoretical basis for the alteration of the microbial community caused by the perchlorate pollution, which maybe have a truly important impact on the quality of groundwater.

Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs invitro.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel bunyavirus (SFTS virus, SFTSV). At present there is still no specific antiviral treatment for SFTSV; To understand which cells support SFTSV life cycle and whether SFTSV infection activates host innate immunity, four different cell lines (Vero, Hela, Huh7.5.1, and Huh7.0) were infected with SFTSV. Intracellular/extracellular viral RNA and expression of IFNα, and IFNß were detected by real-time RT- PCR following infection. To confirm the role of non-structural protein (NSs) of SFTSV in exogenous IFNα-induced Jak/STAT signaling, p-STAT1 (Western Blot), ISRE activity (Luciferase assay) and ISG expression (real-time PCR) were examined following IFNα stimulation in the presence or absence of over-expression of NSs in Hela cells. Our study showed that all the four cell lines supported SFTSV life cycle and SFTSV activated host innate immunity to produce type I IFNs in Hela cells but not in Huh7.0, Huh7.5.1 or Vero cells. NSs inhibited exogenous IFNα-induced Jak/STAT signaling as shown by decreased p-STAT1 level, suppressed ISRE activity and down-regulated ISG expression. Suppression of the exogenous Type I IFN-induced Jak/STAT signaling by NSs might be one of the mechanisms of SFTSV to evade host immune surveillance.

Nonstructural protein (NS1) of human parvovirus B19 stimulates host innate immunity and blunts the exogenous type I interferon signaling in vitro.

B19 virus is a non-enveloped DNA virus and belongs to the family of parvoviridae. There are two large open reading frames (ORFs), nonstructural protein (NS1) and two capsid proteins (VP1 and VP2). Host innate immune responses form the first line of defense against many pathogen invasion. How B19 virus, especially its encoded viral proteins interacts with host innate immune system remains unknown. In this study we aim to investigate the effect of NS1 on the host innate immune response and exogenous type I IFN signaling. Here we found that the type I IFN can be stimulated by NS1. Interestingly, NS1 also plays an important role in inhibiting the exogenous type I IFN signaling at p-STAT1, ISRE and ISGs levels. We concluded that NS1 may play pivotal role in evading the host immune surveillance. Our data shed novel light on the pathogenesis of B19 viral infection and virus evasion strategies.