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1.
Sensors (Basel) ; 23(24)2023 Dec 05.
Artículo en Inglés | MEDLINE | ID: mdl-38139488

RESUMEN

To achieve omnidirectional sensitive detection of partial discharge (PD) in transformers and to avoid missing PD signals, a fiber optic omnidirectional sensing method for PD in transformers combined with the fiber Bragg grating (FBG) and Fabry-Perot (F-P) cavity is proposed. The fiber optic omnidirectional sensor for PD as a triangular prism was developed. The hollow structure of the probe was used to insert a single-mode fiber to form an F-P cavity. In addition, the three sides of the probe were used to form a diaphragm-type FBG sensing structure. The ultrasound sensitization diaphragm was designed based on the frequency characteristics of PD in the transformer and the vibration model of the diaphragm in the liquid environment. The fiber optic sensing system for PD was built and the performance test was conducted. The results show that the resonant frequency of the FBG acoustic diaphragm is around 20 kHz and that of the F-P cavity acoustic diaphragm is 94 kHz. The sensitivity of the developed fiber optic sensor is higher than that of the piezoelectric transducer (PZT). The lower limit of PD detection is 68.72 pC for the FBG sensing part and 47.97 pC for the F-P cavity sensing part. The directional testing of the sensor and its testing within a transformer simulation model indicate that the proposed sensor achieves higher detection sensitivity of PD in all directions. The omnidirectional partial discharge ultrasound sensing method proposed in this paper is expected to reduce the missed detection rate of PD.

2.
Sci Total Environ ; 838(Pt 4): 156476, 2022 Sep 10.
Artículo en Inglés | MEDLINE | ID: mdl-35679942

RESUMEN

Typhoon disasters have caused casualties, property loss, and other negative impacts to social and economic development. Vulnerability is an important component of typhoon risk. However, little is known about the contributions of vulnerability factors and their interaction effects on typhoon-induced losses at a fine scale. Focusing on the vulnerability measures of Typhoon Hato in 2017 and Typhoon Mangkhut in 2018, this study aims to quantify the contribution and interactive effects of physical and socioeconomic factors on vulnerability based on the GeoDetector method and determine the factors that account for most of the change in vulnerability. The results show that from Typhoon Hato in 2017 to Typhoon Mangkhut in 2018, the vulnerability of the economy and houses decrease on average. Rain intensity and wind intensity are the dominant factors of disaster loss for Typhoon Hato and Typhoon Mangkhut, respectively. Vegetation cover and landform explain vulnerability better than average slope in most instances. For different loss types, the dominant socioeconomic vulnerability factor is different. For both typhoons, emergency transfer has a higher determining power (q) ranking for the population vulnerability, while the percentage of the GDP made up of primary industry have higher q ranking for economic vulnerability. The dominant interaction effects between two vulnerability factors differ depending on the typhoon and loss type but show a nonlinear enhancement effect in most cases. Moreover, changes in the maximum 4-hour accumulated rainfall account for most of the change in vulnerability between Hato and Mangkhut. Overall, the results can be conducive to understanding the complexity of vulnerability to typhoons and provide a reference for possible indicators for vulnerability assessment models, and determining the reasons for changes in vulnerability can be constructive to the formulation of specific policies for disaster prevention and mitigation.


Asunto(s)
Tormentas Ciclónicas , Desastres , Vulnerabilidad Social , Vivienda/clasificación , Vivienda/normas , Lluvia , Factores Socioeconómicos , Viento
3.
Mol Biotechnol ; 64(1): 57-65, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34532832

RESUMEN

Arginine decarboxylase (ADC) catalyzes the decarboxylation of arginine to form agmatine, an important physiological and pharmacological amine, and attracts attention to the enzymatic production of agmatine. In this study, we for the first time overexpressed and characterized the marine Shewanella algae ADC (SaADC) in Escherichia coli. The recombinant SaADC showed the maximum activity at pH 7.5 and 40 °C. The SaADC displayed previously unreported substrate inhibition when the substrate concentration was higher than 50 mM, which was the upper limit of testing condition in other reports. In the range of 1-80 mM L-arginine, the SaADC showed the Km, kcat, Ki, and kcat/Km values of 72.99 ± 6.45 mM, 42.88 ± 2.63 s-1, 20.56 ± 2.18 mM, and 0.59 s/mM, respectively, which were much higher than the Km (14.55 ± 1.45 mM) and kcat (12.62 ± 0.68 s-1) value obtained by assaying at 1-50 mM L-arginine without considering substrate inhibition. Both the kcat values of SaADC with and without substrate inhibition are the highest ones to the best of our knowledge. This provides a reference for the study of substrate inhibition of ADCs.


Asunto(s)
Proteínas Bacterianas/metabolismo , Carboxiliasas/metabolismo , Escherichia coli/genética , Shewanella/enzimología , Agmatina/metabolismo , Arginina/metabolismo , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Carboxiliasas/química , Carboxiliasas/genética , Carboxiliasas/aislamiento & purificación , Codón , Expresión Génica , Concentración de Iones de Hidrógeno , Cinética , Modelos Moleculares , Estabilidad Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Temperatura
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