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To achieve food security in a contaminated agricultural land, the remediation areas usually need more samples to obtain accurate contamination information and implement appropriate measures. In this study, we propose an optimal encryption sampling design to instead of the detailed survey, which is determined by the variation of heavy metals and the technology capability of remediation, to guide soil sampling for accurately remediation in the potential remediation-effective areas (PRA). The coefficient of screening variation threshold (CSVT), considering spatial variation, technology capacity and acceptable error of sampling, together with the spatial cyclic statistics method of neighbourhood analysis, is introduced to identify and delineate the PRA. Both of the hypothetical analysis and application case studies are conducted to illustrate the advantages and disadvantages of the optimization. The results show that, compared with the detailed survey, the optimal design shows a lower overall accuracy due to its sparsely sampling at the clean area, but it exhibits a similar effect of accurately prediction in boundary delineation and further classification in the PRA in both simulation and application studies. This work provides an effective method for subsequent accurate remediation at the investigation stage and valuable insights into application combination of technology capacity and contaminated agricultural land investigation.
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Agricultura , Monitoreo del Ambiente , Restauración y Remediación Ambiental , Contaminantes del Suelo , Contaminantes del Suelo/análisis , Restauración y Remediación Ambiental/métodos , Monitoreo del Ambiente/métodos , Suelo/química , Metales Pesados/análisisRESUMEN
Coix lacryma-jobi L. is a traditional medicinal plant in east Asia and is an important crop in Guizhou province, southwest China, where there are elevated levels of soil mercury and arsenic (As). Exposure to multiple potentially toxic elements (PTEs) may affect plant accumulation of metal(loid)s and food safety in regions with high geological metal concentrations. Field experiments were conducted to study the effects of PTEs on metal(loid) accumulation and physiological response of C. lacryma in different plant parts at three pollution levels. Total root length, number of root tips, number of branches, and number of root crosses increased with increasing pollution level, with increases in highly polluted areas of 44.2, 57.0, 79.6, and 97.2%, respectively, compared to lightly polluted areas. Under multi-element stress the activity of C. lacryma antioxidant oxidase showed an increase at low and medium PTE concentrations and inhibition at high concentrations. The As contents were all below the maximum limit of cereal food contaminants in China (GB 2762-2022, As < 0.5 mg kg-1). The stems had high Tl bioconcentration factors but the translocation factors from stem to grain were very low, indicating that the stems may be a key plant part restricting Tl transport to the grains. C. lacryma increased root retention and reduced the transport effect, thus reducing metal accumulation in the grains. C. lacryma adapted to PTE stress through root remodeling and enhanced antioxidant enzyme activities.
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Minería , Contaminantes del Suelo , Contaminantes del Suelo/toxicidad , Contaminantes del Suelo/metabolismo , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Suelo/química , China , Arsénico/toxicidad , Arsénico/metabolismo , Mercurio/toxicidad , Mercurio/metabolismo , Mercurio/análisisRESUMEN
Soil thallium (Tl) contamination is of major public concern but little is known about soil Tl ecological toxicity or potential ecological remediation strategies. Here, two soil animal species with different ecological niches, Folsomia candida and Enchytraeus crypticus, were used to test Tl toxicity and modification by exogenous organic materials (i.e. maize straw and biochar). The endpoints of Tl ecotoxicity to F. candida and E. crypticus were studied at two biological levels, i.e., the individual (body Tl concentrations) and the population (survival, reproduction, and growth). Thallium concentrations in F. candida and E. crypticus increased with increasing soil Tl concentration, and their survival and reproduction rates decreased with increasing soil Tl concentration. The LC50 value of Tl effects on F. candida mortality (28 d) was 24.0 mg kg-1 and the EC50 value of reproduction inhibition was 6.51 mg kg-1. The corresponding values were 4.15 mg kg-1 and 2.31 mg kg-1 respectively for E. crypticus showing higher sensitivity to soil Tl than F. candida. These effective values are comparable to or much lower than the environmental Tl concentrations in field soils, suggesting high potential ecological risk. Both biochar and straw can decrease animal body Tl concentrations in different ways, i.e. reducing Tl availability or offering clean food sources, and addition of exogenous organic materials clearly mitigated Tl ecotoxicity in highly polluted soil. The results highlight the potential Tl ecological risk to soil animals and the potential use of organic materials to control the toxicity.
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Oligoquetos , Contaminantes del Suelo , Talio , Animales , Talio/toxicidad , Contaminantes del Suelo/toxicidad , Oligoquetos/efectos de los fármacos , Suelo/química , Carbón Orgánico , Zea maysRESUMEN
Sedum plumbizincicola is a renowned hyperaccumulator of cadmium (Cd), possesses significant potential for eco-friendly phytoremediation of soil contaminated with Cd. Nevertheless, comprehension of the mechanisms underpinning its Cd stress response remains constrained, primarily due to the absence of a comprehensive genome sequence and an established genetic transformation system. In this study, we successfully identified a novel protein that specifically responds to Cd stress through early comparative iTRAQ proteome and transcriptome analyses under Cd stress conditions. To further investigate its structure, we employed AlphaFold, a powerful tool for protein structure prediction, and found that this newly identified protein shares a similar structure with Arabidopsis AtSIZ1. Therefore, we named it Sedum plumbizincicola SIZ1 (SpSIZ1). Our study revealed that SpSIZ1 plays a crucial role in positively regulating Cd tolerance through its coordination with SpABI5. Overexpression of SpSIZ1 significantly enhanced plant resistance to Cd stress and reduced Cd accumulation. Expression pattern analysis revealed higher levels of SpSIZ1 expression in roots compared to stems and leaves, with up-regulation under Cd stress induction. Importantly, overexpressing SpSIZ1 resulted in lower Cd translocation factors (Tfs) but maintained relatively constant Cd levels in roots under Cd stress, leading to enhanced Cd stress resistance in plants. Protein interaction analysis revealed that SpSIZ1 interacts with SpABI5, and the expression of genes responsive to abscisic acid (ABA) through SpABI5-dependent signaling was significantly up-regulated in SpSIZ1-overexpressing plants with Cd stress treatment. Collectively, our results illustrate that SpSIZ1 interacts with SpABI5, enhancing the expression of ABA downstream stress-related genes through SpABI5, thereby increasing Cd tolerance in plants.
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Cadmium (Cd) is a heavy metal pollutant mainly originating from the discharge of industrial sewage, irrigation with contaminated water, and the use of fertilizers. The phytoremediation of Cd polluted soil depends on the identification of the associated genes in hyperaccumulators. Here, a novel Cd tolerance gene (SpCTP3) was identified in hyperaccumulator Sedum plumbizincicola. The results of Cd2+ binding and thermodynamic analyses, revealed the CXXC motif in SpCTP3 functions is a Cd2+ binding site. A mutated CXXC motif decreased binding to Cd by 59.93%. The subcellular localization analysis suggested that SpCTP3 is primarily a cytoplasmic protein. Additionally, the SpCTP3-overexpressing (OE) plants were more tolerant to Cd and accumulated more Cd than wild-type Sedum alfredii (NHE-WT). The Cd concentrations in the cytoplasm of root and leaf cells were significantly higher (53.75% and 71.87%, respectively) in SpCTP3-OE plants than in NHE-WT. Furthermore, malic acid levels increased and decreased in SpCTP3-OE and SpCTP3-RNAi plants, respectively. Moreover, SpCTP3 interacted with malate dehydrogenase 1 (MDH1). Thus, SpCTP3 helps regulate the subcellular distribution of Cd and increases Cd accumulation when it is overexpressed in plants, ultimately Cd tolerance through its interaction with SpMDH1. This study provides new insights relevant to improving the Cd uptake by Sedum plumbizincicola.
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Biodegradación Ambiental , Cadmio , Proteínas de Plantas , Sedum , Contaminantes del Suelo , Cadmio/toxicidad , Cadmio/metabolismo , Sedum/metabolismo , Sedum/genética , Sedum/efectos de los fármacos , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Contaminantes del Suelo/toxicidad , Contaminantes del Suelo/metabolismo , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Plantas Modificadas Genéticamente/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Malato Deshidrogenasa/metabolismo , Malato Deshidrogenasa/genéticaRESUMEN
Abundant naturally and anthropogenically exposed surrounding rocks (NESRs and AESRs) in mining areas may pose persistent threats as sources of potentially toxic elements (PTEs), but this has been historically overlooked, especially for thallium (Tl) and arsenic (As). Here, the release risks of Tl and As from both NESRs and AESRs in a typical TlAs sulfide mining area were investigated. In a single leaching process, AESRs released 10.4 % of total Tl (157 µg L-1) and 32.5 % of total As (4089 µg L-1), 2-3 orders of magnitude higher than NESRs. Prolonged multiple leaching tests revealed notable and long-term risks of release of Tl and As from AESRs, associated with oxidation and dissolution of iron/sulfur-bearing minerals. Substantial release of PTEs was linked to the transformation/degradation of the -OH functional group and extensive dissolution of secondary sulfate minerals in AESRs. Ultrafiltration and STEM-EDS indicate that 18.4 % of water-extracted As released from AESRs existed as natural nanoparticles consisting of iron/sulfur-bearing minerals. This study highlights the high risks of Tl and As release from anthropogenically exposed surrounding rocks and the importance of nanoparticles in PTE transport, and provides insights into the control of PTEs in mining areas.
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Accurate prediction of cadmium (Cd) ecotoxicity to and accumulation in soil biota is important in soil health. However, very limited information on Cd ecotoxicity on naturally contaminated soils. Herein, we investigated soil Cd ecotoxicity using Folsomia candida, a standard single-species test animal, in 28 naturally Cd-contaminated soils, and the back-propagation neural network (BPNN) model was used to predict Cd ecotoxicity to and accumulation in F. candida. Soil total Cd and pH were the primary soil properties affecting Cd toxicity. However, soil pH was the main factor when the total Cd concentration was < 3 mg kg-1. Interestingly, correlation analysis and the K-spiked test confirmed nutrient potassium (K) was essential for Cd accumulation, highlighting the significance of studying K in Cd accumulation. The BPNN model showed greater prediction accuracy of collembolan survival rate (R2 = 0.797), reproduction inhibitory rate (R2 = 0.827), body Cd concentration (R2 = 0.961), and Cd bioaccumulation factor (R2 = 0.964) than multiple linear regression models. Then the developed BPNN model was used to predict Cd ecological risks in 57 soils in southern China. Compared to multiple linear regression models, the BPNN models can better identify high-risk regions. This study highlights the potential of BPNN as a novel and rapid tool for the evaluation and monitoring of Cd ecotoxicity in naturally contaminated soils.
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Artrópodos , Contaminantes del Suelo , Animales , Cadmio/toxicidad , Cadmio/análisis , Suelo/química , Contaminantes del Suelo/toxicidad , Contaminantes del Suelo/análisis , Redes Neurales de la Computación , ReproducciónRESUMEN
East Yunnan province in southwest China is a region with elevated natural abundance (high geological background levels) of Cd due to high metal (loid) contents in the soils. Enzyme activities are useful indicators of metal (loid) toxicity in contaminated soils and whether Cd inhibits enzyme activities in paddy soils in high geological background areas is of considerable public concern. A pot experiment combined with field investigation was conducted to assess the effects of Cd on six soil enzymes that are essential to the cycling of C, N, and P in soils. Inhibitory effects of Cd fractions on enzyme activities were assessed using ecological dose-response models. The impact of soil properties on the inhibition of sensitive soil enzymes by Cd were assessed using linear and structural equation models. Cadmium was enriched in the paddy soils with 72.2 % of soil samples from high geological background areas exceeding the Chinese threshold values (GB 15618-2018) of Cd. Enzyme responses to Cd contamination varied markedly with a negative response by catalase but a positive response by invertase. Urease, ß-glucosidase, and alkaline phosphatase activities were stimulated at low Cd concentrations and inhibited at high concentrations. The average inhibition ratios of ß-glucosidase, urease, and catalase in high Cd levels were 19.9, 38.9, and 51.9%, respectively. Ecological dose-response models indicate that catalase and urease were the most Cd-sensitive of the enzymes studied and were suitable indicators of soil quality in high geological background areas. Structural equation modeling (SEM) indicates that soil properties influenced sensitive enzymes through various pathways, indicating that soil properties were factors determining Cd inhibition of enzyme activities. This suggests that Cd concentrations and soil physicochemical properties under a range of environmental conditions should be considered in addressing soil Cd pollution.
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Celulasas , Oryza , Contaminantes del Suelo , Cadmio/análisis , Suelo/química , Catalasa , Ureasa/metabolismo , Contaminantes del Suelo/análisis , China , Oryza/metabolismoRESUMEN
BACKGROUND AND AIMS: Kalanchoideae is one of three subfamilies within Crassulaceae and contains four genera. Despite previous efforts, the phylogeny of Kalanchoideae remains inadequately resolved with persistent issues including low support, unstructured topologies and polytomies. This study aimed to address two central objectives: (1) resolving the pending phylogenetic questions within Kalanchoideae by using organelle-scale 'barcodes' (plastomes) and nuclear data; and (2) investigating interspecific diversity patterns among Kalanchoideae plastomes. METHODS: To explore the plastome evolution in Kalanchoideae, we newly sequenced 38 plastomes representing all four constituent genera (Adromischus, Cotyledon, Kalanchoe and Tylecodon). We performed comparative analyses of plastomic features, including GC and gene contents, gene distributions at the IR (inverted repeat) boundaries, nucleotide divergence, plastomic tRNA (pttRNA) structures and codon aversions. Additionally, phylogenetic inferences were inferred using both the plastomic dataset (79 genes) and nuclear dataset (1054 genes). KEY RESULTS: Significant heterogeneities were observed in plastome lengths among Kalanchoideae, strongly correlated with LSC (large single copy) lengths. Informative diversities existed in the gene content at SSC/IRa (small single copy/inverted repeat a), with unique patterns individually identified in Adromischus leucophyllus and one major Kalanchoe clade. The ycf1 gene was assessed as a shared hypervariable region among all four genera, containing nine lineage-specific indels. Three pttRNAs exhibited unique structures specific to Kalanchoideae and the genera Adromischus and Kalanchoe. Moreover, 24 coding sequences revealed a total of 41 lineage-specific unused codons across all four constituent genera. The phyloplastomic inferences clearly depicted internal branching patterns in Kalanchoideae. Most notably, by both plastid- and nuclear-based phylogenies, our research offers the first evidence that Kalanchoe section Eukalanchoe is not monophyletic. CONCLUSIONS: This study conducted comprehensive analyses on 38 newly reported Kalanchoideae plastomes. Importantly, our results not only reconstructed well-resolved phylogenies within Kalanchoideae, but also identified highly informative unique markers at the subfamily, genus and species levels. These findings significantly enhance our understanding of the evolutionary history of Kalanchoideae.
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Crassulaceae , Filogenia , Crassulaceae/genética , Plastidios/genética , Evolución Biológica , Evolución Molecular , Genoma de PlastidiosRESUMEN
Sedum plumbizincicola is a cadmium (Cd) and zinc hyperaccumulator that can activate Cd by rhizosphere acidification. However, there is little understanding of the Cd leaching risk from polluted soil during phytoextraction process. Here, pot and column experiments were conducted to monitor soil Cd leaching characteristics under different rainfall simulation conditions during S. plumbizincicola phytoextraction. Soil Cd leaching increased significantly with increasing simulated rainfall intensity. Compared with normal rainfall (NR), weak rainfall (WR) resulted in a 34.3% decrease in Cd uptake by S. plumbizincicola and also led to a 68.7% decline in Cd leaching. In contrast, Cd leaching under heavy rainfall (HR) was 2.12 times that of NR in the presence of S. plumbizincicola. After two successive growing periods, phytoextraction resulted in a 53.5-66.4% decline in the amount of soil Cd leached compared with controls in which S. plumbizincicola was absent. Even compared with maize cropping as a control, S. plumbizincicola did not instigate a significant increase in Cd leaching. The contribution of Cd leaching loss to the decline in soil total Cd concentration was negligible after phytoextraction in the pot experiment. Overall, the results contribute to our understanding of soil Cd leaching risk by phytoextraction with S. plumbizincicola.
Repeated phytoextraction by hyperaccumulator Sedum plumbizincicola is an important remediation technology to remove Cd from contaminated soils. At the same time, Sedum plumbizincicola can also activate soil Cd by rhizosphere acidification. However, studies on the leaching risk of soil activated Cd during the phytoextraction process are very few. This study looked at the effects of Sedum plumbizincicola growth on soil Cd leaching with the changes in rainfall simulation and plant type. Results showed that repeated phytoextraction with Sedum plumbizincicola did not increase Cd leaching from contaminated soil.
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Sedum , Contaminantes del Suelo , Cadmio , Contaminantes del Suelo/análisis , Biodegradación Ambiental , SueloRESUMEN
The root-associated microbiome assembly substantially promotes (hyper)accumulator plant growth and metal accumulation and is influenced by multiple factors, especially host species and environmental stress. Athyrium wardii (Hook.) is a phytostabilizer that grows in lead (Pb)-zinc (Zn) mine tailings and shows high root Pb accumulation. However, there remains little information on the assembly of the root-associated microbiome of A. wardii and its role in phytostabilization. A field study investigated the structural and functional variation in the root-associated bacterial microbiome of Athyrium wardii (Hook.) exposed to different levels of contamination in Pb-Zn mine tailings. The root compartment dominated the variation in the root-associated bacterial microbiome but the levels of contaminants showed less impact. Bacterial co-occurrence was enhanced in the rhizosphere soil and rhizoplane but tended to be much simpler in the endosphere in terms of network complexity and connectivity. This indicates that the microbial community assembly of A. wardii was non-random and shaped by root selective effects. Proteobacteria, Chloroflexi, Actinobacteria, Cyanobacteria, and Acidobacteriota were generally the dominant bacterial phyla. The genera Crossiella and Bradyrhizobium were enriched in the rhizosphere and cyanobacterial genera were enriched in the endosphere, demonstrating substantial advantages to plant survival and adaptation in the harsh mine environment. Functional categories involved in amino acid and carbohydrate metabolism were abundant in the rhizosphere soil, thus contributing to metal solubility and bioavailability in the rhizosphere. Membrane transporters, especially ATP-binding cassette transporters, were enriched in the endosphere, indicating a potential role in metal tolerance and transportation in A. wardii. The study shows substantial variation in the structure and function of microbiomes colonizing different compartments, with the rhizosphere and endophytic microbiota potentially involved in plant metal tolerance and accumulation during phytostabilization.
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Microbiota , Tracheophyta , Plomo/toxicidad , Plomo/metabolismo , Plantas , Bacterias , Zinc/toxicidad , Zinc/metabolismo , Suelo/química , Rizosfera , Raíces de Plantas/metabolismo , Microbiología del SueloRESUMEN
Water-dispersible colloids (WDCs) are vital for trace element migration, but there is limited information about the abundance, size distribution and elemental composition of WDC-bound thallium (Tl) and arsenic (As) in mining-contaminated soils and sediments solutions. Here, we investigated the potential mobilization of WDC-bound Tl and As in soils and sediments in a typical Tl/As-contaminated area. Ultrafiltration results revealed on average > 60% of Tl and As in soil solution (< 220 nm) coexisted in colloidal form whereas Tl and As in sediment solution primarily existed in the truly dissolved state (< 10 kDa) due to increased acidity. Using AF4-UV-ICP-MS and STEM-EDS, we identified Fe-bearing WDCs in association with aluminosilicate minerals and organic matter were main carriers of Tl and As. SAED further verified jarosite nanoparticles were important components of soil WDC, directly participating in the migration of Tl and As. Notably, high pollution levels and solution pH promoted the release of Tl/As-containing WDCs. This study provides quantitative and visual insights into the distribution of Tl and As in WDC, highlighting the important roles of Fe-bearing WDC, soil solution pH and pollution level in the potential mobilization of Tl and As in contaminated soils and sediments.
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To explore the safe utilization technology of farmland polluted by the heavy metals cadmium (Cd) and lead (Pb) and to realize the safe production of agricultural products, a pot experiment was conducted to investigate the effects of two soil passivators and five foliar inhibitors on Cd and Cd-accumulation and quality of lettuce with low Pb and Cd accumulation (KCW). The results showed that different control measures had different effects on the soil pH value of lettuce, and the application of 45 g·m-2biochar-based passivator had the most significant difference in improving the soil pH value, which was increased by 0.8 units compared with that in CK. By using 72 g·m-2 of humic acid passivator yielded notable difference in reducing the soil pH value of lettuce. A reduction of 0.25 units was achieved compared with that in CK. Among all the control measures, the application of 45 g·m-2 biocharcoal-based passivation agent had the best effect on reducing soil available Cd content, which was significantly reduced by 53% compared with that in CK, and the application of 135 g·m-2biocharcoal-based passivation agent had the best effect on reducing soil available Pb content, which was significantly reduced by 64% compared with that in CK. Spraying 0.8% FAK-Zn foliar inhibitor not only had the best control effect on reducing Cd and Pb contents in the edible parts of lettuce, which were significantly reduced by 77% and 60%, respectively, compared with that in CK, but it also significantly reduced Cd and Pb enrichment coefficients and transport coefficients from the root to the edible parts of the lettuce. Different control measures had different effects on the nutritional quality of lettuce, and 0.4% FAK-Zn foliar inhibitor had the best effect on soluble protein. The 0.6% FAK-Zn had the best effect on soluble sugar, and the 0.4% FAK-Zn inhibitor had the best effect on vitamin C content. The application of biocarbon-based passivator could effectively repair lettuce soil polluted by Cd and Pb, whereas the application of FAK-Zn leaf surface inhibitor could effectively inhibit the accumulation, absorption, and transfer of Cd and Pb in lettuce; improve the nutritional quality of lettuce; provide a theoretical basis for safe production of vegetables polluted by heavy metals; and promote the recycling of resources and environment.
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Cadmio , Lactuca , Plomo , Verduras , SueloRESUMEN
Phytoextraction with Sedum plumbizincicola is an in-situ, environmentally friendly and highly efficient remediation technique for slightly Cd-polluted soils but it remains a challenge to remediate highly Cd-polluted soils under field conditions. Here, an 8-ha field experiment was conducted to evaluate the feasibility of repeated phytoextraction by S. plumbizincicola of a highly Cd-polluted acid agricultural soil (pH 5.61, [Cd] 2.58 mg kg-1) in Yunnan province, southwest China. Mean shoot dry biomass production, Cd concentration and Cd uptake were 1.95 t ha-1, 170 mg kg-1 and 339 g ha-1 at the first harvest, and 0.91 t ha-1, 172 mg kg-1 and 142 g ha-1 at the second harvest. After two seasons of phytoextraction, soil total and CaCl2-extractable Cd concentrations decreased from 2.58 ± 0.69 to 1.53 ± 0.43 mg kg-1 and 0.22 ± 0.12 to 0.14 ± 0.07 mg kg-1, respectively. Stepwise multiple linear regression analysis shows that the shoot Cd concentration and uptake of S. plumbizincicola were positively related to soil CaCl2-extractable Cd concentrations, especially in the first crop. A negative relationship indicates that soil organic matter content played an important role in soil Cd availability and shoot Cd concentration in the first crop. In addition, the rhizosphere effect on soil CaCl2-extractable Cd concentration was negatively correlated with soil pH in the first crop. The accuracy of the calculation of soil Cd phytoextraction efficiency at field scale depends on all of the following factors being considered: shoot Cd uptake, cropping pattern, standardized sampling points, and the leaching and surface runoff of Cd. Phytoextraction with S. plumbizincicola is a feasible technique for efficient Cd removal from highly polluted soils and wide variation in soil properties can influence phytoextraction efficiency at the field scale.
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Sedum , Contaminantes del Suelo , Cadmio/análisis , Zinc/análisis , Sedum/química , Cloruro de Calcio , Contaminantes del Suelo/análisis , Biodegradación Ambiental , China , Suelo/químicaRESUMEN
The association of arsenic (As) with colloidal particles could facilitate its transport to adjacent water systems or alter its availability in soil-rice systems. However, little is known about the size distribution and composition of particle-bound As in paddy soils, particularly under changing redox conditions. Here, we incubated four As-contaminated paddy soils with distinctive geochemical properties to study the mobilization of particle-bound As during soil reduction and subsequent reoxidation. Using transmission electron microscopy-energy dispersive spectroscopy and asymmetric flow field-flow fractionation, we identified organic matter (OM)-stabilized colloidal Fe, most likely in the form of (oxy)hydroxide-clay composite, as the main arsenic carriers. Specifically, colloidal As was mainly associated with two size fractions of 0.3-40 and >130 kDa. Soil reduction facilitated the release of As from both fractions, whereas reoxidation caused their rapid sedimentation, coinciding with solution Fe variations. Further quantitative analysis demonstrated that As concentrations positively correlated with both Fe and OM concentrations at nanometric scales (0.3-40 kDa) in all studied soils during reduction and reoxidation, yet the correlations are pH-dependent. This study provides a quantitative and size-resolved understanding of particle-bound As in paddy soils, highlighting the importance of nanometric Fe-OM-As interactions in paddy As geochemical cycling.
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Arsénico , Oryza , Contaminantes del Suelo , Arsénico/química , Contaminación Ambiental/análisis , Suelo/química , Coloides/metabolismoRESUMEN
Soil metal(loid)s in high geological background areas occur mainly in the residual form with low bioavailability, and whether these potentially toxic elements (PTEs) in agricultural soils are harmful to human health is of considerable public concern. A paired survey using both soil and crop samples was conducted using 437 contaminated sites in east Yunnan province, southwest China. The concentration, distribution, and source of PTEs (arsenic (As), copper (Cu), zinc (Zn), lead (Pb), cadmium (Cd), and chromium (Cr)) in agricultural soils, and the contamination levels of and potential health risks from PTEs from different pollution sources were evaluated. Soil Pb, Cu, Zn, Cd, Cr, and As concentrations were higher than the Chinese screening values (GB 15618-2018) of 10.98, 36.16, 24.71, 86.96, 14.19, and 6.64 %, respectively, and Cd greatly exceeded the screening values. Spatial distribution maps indicate that areas with high concentrations of Pb, Cu, Cd, and As were located mainly in mining areas. However, the Zn and Cr concentrations were relatively homogeneous and more dependent on natural processes. The source identification of PTEs shows that Zn and Cr in soils were controlled mainly by the geological background, Pb and As were closely related to anthropogenic activities, and Cu and Cd were related to both sources. Different pollution sources affected crop PTE contents, with average concentrations of Zn, Cd, Cr, and As in high geological background areas significantly lower than in anthropogenic activity areas (p < 0.001), while Cu and As did not differ significantly. Although soil PTEs in high geological background areas represent a relatively high potential risk, they had little impact on crop quality. The hazard indices of different crop products for adults and children followed the sequence: cereals > leafy vegetables > rootstalk vegetables > fruit vegetables. Rootstalk and fruit vegetables are recommended to be grown in the study areas because they are safe for human consumption.
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Arsénico , Metales Pesados , Contaminantes del Suelo , Adulto , Niño , Humanos , Metales Pesados/análisis , Suelo , Cadmio , Plomo , Monitoreo del Ambiente , Contaminantes del Suelo/análisis , China , Arsénico/análisis , Zinc , Verduras , Cromo , Medición de RiesgoRESUMEN
This study employs stable isotope analysis to investigate the mechanisms of cadmium (Cd) and zinc (Zn) interaction in the metal hyperaccumulating plant species Sedum plumbizincicola. To this end, the Cd and Zn isotope compositions of root, stem, leaf, and xylem sap samples were determined during metal uptake and translocation at different Cd and Zn concentrations. The enrichment of light isotopes of both elements in plants during uptake was less pronounced at low metal supply levels, likely reflecting the switch from a low-affinity to a high-affinity transport system at lower levels of external metal supply. The lower δ114/110Cd values of xylem sap when treated with a metabolic inhibitor decreasing the active Cd uptake further supports the preference of heavier Cd isotopes during high-affinity transport. The Δ66Znplant-initial solution or Δ66Znplant-final solution values were similar at different Cd concentrations, indicating negligible interaction of Cd in the Zn uptake process. However, decreasing Zn supply levels significantly increased the enrichment of light Cd isotopes in plants (Δ114/110Cd = -0.08) in low-Cd treatments but reduced the enrichment of light Cd isotopes in plants (Δ114/110Cd = 0.08) under high Cd conditions. A systematic enrichment of heavy Cd and light Zn isotopes was found in root-to-shoot translocation of the metals. The Cd concentrations of the growth solutions thereby had no significant impact on Zn isotope fractionation during root-to-shoot translocation. However, the Δ114/110Cdtranslocation values hint at possible competition between Cd and Zn for transporters during root-to-shoot transfer and this may impact the transport pathway of Cd. The stable isotope data demonstrate that the interactions between the two metals influenced the uptake and transport mechanisms of Cd in S. plumbizincicola but had little effect on those of Zn.
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Cadmio , Sedum , Contaminantes del Suelo , Suelo , Biodegradación Ambiental , Cadmio/análisis , Cadmio/metabolismo , Isótopos/análisis , Isótopos/metabolismo , Isótopos/farmacología , Raíces de Plantas/química , Raíces de Plantas/metabolismo , Sedum/metabolismo , Contaminantes del Suelo/análisis , Contaminantes del Suelo/metabolismo , Zinc/análisis , Isótopos de Zinc/análisis , Isótopos de Zinc/metabolismo , Isótopos de Zinc/farmacologíaRESUMEN
Soil pollution represents a threat to soil biodiversity and to soil and human health. However, many ecotoxicological issues, such as the impact of heavy metal pollution on the soil mite community and its spatial distribution in areas with complex environmental factors, are not fully understood. Here, an investigation was conducted in an arable area (about 11 km2) enclosed by surrounding mountains. The study area was contaminated with potentially toxic metals derived from copper smelting that was functioning for over 10 years. The area comprised four land use types: woodlands, dry fields, paddy fields, and wastelands, and was divided into 141 study sites each with an area of 6.25 ha. The soil metal (Cu, Zn, Pb, and Cd) contents, pH, and organic matter were determined and their distributions were established. Furthermore, soil mite (Acari) community properties (species richness, individual abundance, and Shannon-Wiener diversity index) were determined, and the distributions of total species number and abundance were ascertained. Soil metal pollution strongly reduced soil mite community, but the effects depended on mite groups or species and their sensitivity to different metals as well as land use types. CANOCO analysis revealed that the order Oribatida was more highly correlated with soil metal contents, whereas the other three orders responded to soil metal contents depending on land use types, mite properties, or metals. SADIE method indicated that the coordinate relationship between mite species number and metal concentration was more negative (4-25% of the study sites) than positive (4-12%). The metal pollution levels in the soil were evaluated by single and integrated pollution and ecological risk indices.
Asunto(s)
Metales Pesados , Ácaros , Contaminantes del Suelo , Animales , Humanos , Suelo/química , Monitoreo del Ambiente/métodos , Contaminantes del Suelo/análisis , Metales Pesados/análisis , Contaminación Ambiental/análisis , China , Medición de RiesgoRESUMEN
Identification of potential toxic element (PTE) sources and their specific human health risk is critical to the management of PTEs in soils. In this study, multi-medium were collected from a mercurythallium polymetallic mining area in Southwestern China. Hg isotope technique together with positive matrix factorization (PMF) model was used to identify PTE sources and assess their source-oriented health risk. Results showed that among the studied PTEs, this study area presented high pollution of Hg, Tl and As, with higher concentrations than their corresponding background values of Guizhou province, yet their average concentrations in covering soils were significantly lower than those in the natural soils. The Tl in coix grains should also be paid more attention due to its high concentration. Both natural and covering soils had different Hg isotope composition with tailings, while sediments have similar Hg isotope fractionation with covering soils. According to the PMF model, three sources in both natural and covering soils were apportioned and Hg, Tl and As were mainly influenced by the historical mining activities, which also confirmed by their Hg isotope signatures. The contributions of historical mining activities accounted for 40 % and 20 % of the PTEs in natural and covering soils, respectively. The assessment of source-specific health risks suggested that the non-carcinogenic risk of Hg, Tl and As was much higher than other elements. Historical mining activities were regarded as the major contributor to health risks (79 % and 76 % for natural soils and 50 % and 59 % for covering soils, respectively). This indicated that the restoration of coveing soils indeed decreased the health risk in this study area. These findings thus highlight the importance of ongoing monitoring of covering soils in the polymetallic mining area, which is imperative for preferably assessing the health risk of PTEs in similar mining area worldwide.
Asunto(s)
Mercurio , Metales Pesados , Contaminantes del Suelo , Humanos , Mercurio/análisis , Talio , Isótopos de Mercurio , Suelo , Monitoreo del Ambiente/métodos , Contaminantes del Suelo/análisis , China , Medición de Riesgo , Metales Pesados/análisisRESUMEN
Microbial communities are closely related to plant performance and numerous studies have shown their involvement with the growth and development of host plants, resistance to pathogen invasion and adaptation to environmental stress. Here we described in detail the ecological process of the microbial community assembly in hyperaccumulator plant Sedum plumbizincicola. We divided the microbiota into four ecological compartments (bulk soil, rhizosphere, root endosphere and aboveground endosphere). The results showed that host selection strongly controlled the aggregation of microbial community. So that microbes occupied different niches from the bulk soil to the aboveground endosphere, and bacterial diversity and network complexity decreased gradually. Soil types were the second influencing factor, especially for the microbial community in the root endosphere. The SourceTracker analysis further confirmed the vertical migration of microbes from bulk soil to aboveground endosphere. In addition, under the condition of heavy metal pollution, the microbial community of S. plumbizincicola tended to form a microbial pool dominated by Proteobacteria and Actinobacteria. Ellin6067, Sphingomonas, Ralstonia, SC-I-84_uncultured bacterium, Burkholderiaceae_Undibacterium and Pedosphaeraceae_uncultured bacterium etc. were identified as the vital biomarker taxa. Among these genera, the relative abundance of last three was significantly positively correlated with the activation and transfer of cadmium, and they mainly enriched in paddy soil. This study provides evidence for the mechanism by which the microbial community assembly occurs and experience for regulating the microbial community and increasing the accumulation efficiency of potentially toxic metals in S. plumbizincicola.