Efficacy and association of hyperbaric oxygen therapy combined with butylphthalide and oxiracetam with serum levels of inflammatory markers in vascular cognitive impairment after acute ischemic stroke.

Objectives: This study evaluated the efficacy of hyperbaric oxygen therapy (HBOT) combined with butylphthalide (NBP) and oxiracetam (OXR) for vascular cognitive impairment after acute ischemic stroke and investigated the association between such combination therapy and the serum levels of inflammatory markers. Methods: This was a prospective study which included eighty patients with post-AIS cognitive impairment (PAISCI) treated in Dongguan City People's Hospital from January 2020 to January 2022. They were randomized into study group and control group. The control group was provided with conventional therapy consisting of NBP for intravenous transfusion and oral OXR, while the study group received combination therapy of HBOT, NBP, and OXR. A comparison was drawn between the two groups regarding clinical outcomes, levels of recovery of cognitive and neurological function and intelligence, changes in inflammatory markers, and incidence of adverse drug reactions (ADRs). Results: The response rate of the study group was significantly higher than that of the control group (p=0.04). The cognitive function scores of the study group were significantly better than those of the control group at the end of treatment (p<0.05). The post-treatment levels of inflammatory markers were significantly reduced in the study group when compared with the control group (p<0.05). At two weeks after treatment, the ADR rate of study group was significantly lower than the control group (p=0.03). Conclusions: The combination therapy of HBOT, NBP, and OXR demonstrates robust efficacy in patients with PAISCI. It is deemed to be a safe and effective treatment regimen.

Influence of Postoperative Serum Inflammatory Factors, Stress Indicators, Urination Function, Sexual Function and Clinical Efficacy of Laparoscopic Pelvic Floor Reconstruction without Mesh Implantation in the Treatment of Pelvic Organ Prolapse.

Pelvic organ prolapse is seriously harmful to women's health and daily activities, and the incidence rate increases with age, which is more common among middle-aged and elderly women. Common treatment schemes are prone to relapse or complications. The purpose of this article was to study the clinical effect of laparoscopic pelvic floor reconstruction without mesh implantation in the treatment of pelvic organ prolapse and the influence of postoperative serum inflammatory factors, stress indicators, urination function and sexual function. The clinical curative effect of the operation plan was evaluated by the determination of POP-Q value and objective cure rate. The enzyme-linked immunosorbent assay determined the serum inflammatory factors and stress indexes before and after the operation. Urination function was detected by a urodynamics detector, and sexual function was investigated by a PISQ-12 questionnaire. The results show that laparoscopic pelvic floor reconstruction without mesh implantation has good clinical efficacy in the treatment of pelvic organ prolapse, with less stimulation to patients and less inflammation. After the operation, the patient's maximum urine flow rate exceeded 18mL/s, the sexual function score exceeded 45 points, and the urination function and sexual function were effectively improved.

[Review on the speciation analysis methods of platinum group metals in environmental media]. / çŽ¯å¢ƒä»‹è´¨ä¸­é“‚æ—é‡‘å±žå½¢æ€åˆ†æžæ–¹æ³•çš„ç ”ç©¶è¿›å±•.

Platinum group metals (PGMs) present a variety of forms in the environment, and analysis of speciation is essential for identifying their ecological risk. Here, we reviewed the methods for the morphological analysis of three major PGMs (platinum, palladium and rhodium) in the environment, including chemical sequential extraction, hyphenated techniques for instruments, computer simulations. We outlined the types, characteristics and applications of these methods, elaborated the weaknesses, and provided prospects for future development. Among them, chemical sequential extraction is universally applied in the morphological analysis of solid-phase samples, with diverse extraction conditions and procedures proposed in the current study. However, it has not been well standardized. The hyphenated techniques for instruments have significant advantages for the determination of elemental forms in solution, of which capillary electrophoresis system can separate similar substances with the same electrophoresis ability. Liquid chromatography systems have better performance in terms of separation capacity and detection limit. The computer simulations further expand the access to morphological analysis, enabling complex morphological calculations. It was proposed to combine multiple methods in the future to continuously improve the accuracy of analytical techniques by complementing and optimizing each other.

ZIC2 upregulates lncRNA SNHG12 expression to promote endometrial cancer cell proliferation and migration by activating the Notch signaling pathway.

It was previously reported that long non­coding RNA (lncRNA) small nucleolar RNA host gene 12 (SNHG12) promoted the proliferation, invasion and migration of endometrial cancer (EC) cells; however, the upstream underlying mechanism remains unclear. The present study aimed to determine the possible underlying mechanism of SNHG12 regulating EC. The Encyclopedia of RNA Interactomes database was used to analyze whether SNHG12 could bind to Zic family member 2 (ZIC2) and the expression levels of ZIC2 in patients with EC. ZIC2 expression levels in EC cell lines were analyzed using western blotting and reverse transcription­quantitative PCR. RL95­2 cells were subsequently transfected with short hairpin RNA targeting ZIC2, or ZIC2 or SNHG12 overexpression plasmids. Cell proliferation, migration and invasion were analyzed using Cell Counting Kit­8, colony formation, wound healing and Transwell assays, respectively. The binding between ZIC2 and SHNG12 was verified using dual luciferase reporter and chromatin immunoprecipitation assays. The results of the present study revealed that the expression levels of ZIC2 were upregulated in the tissues of patients with EC and EC cell lines. ZIC2 knockdown inhibited RL95­2 cell proliferation, migration and invasion. The protein expression levels of Ki67, proliferating cell nuclear antigen, MMP2 and MMP9 were also downregulated following the knockdown of ZIC2. ZIC2 was predicted to bind to SNHG12 and positively regulate SNHG12 expression. Further experiments demonstrated that the effects of the knockdown of ZIC2 on RL95­2 cells were partially reversed by SNHG12 overexpression. In addition, ZIC2 knockdown inhibited Notch signaling activation, while SNHG12 overexpression reversed this effect. In conclusion, the findings of the present study indicated that ZIC2 may upregulate SNHG12 expression to promote EC cell proliferation and migration by activating the Notch signaling pathway.

Long non­coding RNA SNHG12 regulates cell proliferation, invasion and migration in endometrial cancer by targeting miR­4429.

Long non­coding RNA small nucleolar RNA host gene 12 (SNHG12) has been demonstrated to be oncogenic. The aim of the present study was to examine the effects of SNHG12 on the progression of endometrial cancer (EC). The expression levels of SNHG12 and microRNA (miR)­4429 were assessed in EC cell lines by reverse transcription­quantitative PCR. Plasmids, including SNHG12 short hairpin RNAs (shRNAs), shRNA negative control (NC), SNHG12 overexpression (OV), OV­NC, miR­4429 mimic and mimic­NC, were transfected into RL95­2 cells. Post­transfection, Cell Counting Kit­8, Transwell Matrigel and wound­healing assays were performed to assess cell proliferation, invasion and migration, respectively. Cell cycle phase distribution was assessed by flow cytometry. The protein expression levels of matrix metalloproteinase (MMP)2 and MMP9 were detected by western blotting. miR­4429 target genes were predicted by bioinformatics analysis using target prediction online tools; the findings of this analysis were verified using a dual­luciferase reporter system. Identified as a target of miR­4429, SNHG12 was overexpressed in EC cell lines with decreased expression of miR­4429. Further experiments demonstrated that SNHG12 silencing and overexpression of miR­4429 markedly suppressed proliferation, migration and invasion of RL95­2 cells, arrested cells in the G1 phase, and markedly downregulated the expression of MMP2 and MMP9. The opposite effects were observed in miR­4429 mimic­transfected RL95­2 cells after SNHG12 was overexpressed. The findings of the present study established the role of SNHG12 and miR­4429 in EC. Therefore, targeting the SNHG12/miR­4429 axis could serve as a potential future therapeutic target for treatment of EC.