Application of dissimilatory iron-reducing bacteria for the remediation of soil and water polluted with chlorinated organic compounds: Progress, mechanisms, and directions.

Chlorinated organic compounds are widely used as solvents, but they are pollutants that can have adverse effects on the environment and human health. Dissimilatory iron-reducing bacteria (DIRB) such as Shewanella and Geobacter have been applied to treat a wide range of halogenated organic compounds due to their specific biological properties. Until now, there has been no systematic review on the mechanisms of direct or indirect degradation of halogenated organic compounds by DIRB. This work summarizes the discussion of DIRB's ability to enhance the dechlorination of reaction systems through different pathways, both biological and biochemical. For biological dechlorination, some DIRB have self-dechlorination capabilities that directly dechlorinate by hydrolysis. Adjustment of dechlorination genes through genetic engineering can improve the dechlorination capabilities of DIRB. DIRB can also adjust the capacity for the microbial community to dechlorinate and provide nutrients to enhance the expression of dechlorination genes in other bacteria. In biochemical dechlorination, DIRB bioconverts Fe(III) to Fe(II), which is capable of dichlorination. On this basis, the DIRB-driven Fenton reaction can efficiently degrade chlorinated organics by continuously maintaining anoxic conditions to generate Fe(II) and oxic conditions to generate H2O2. DIRB can drive microbial fuel cells due to their electroactivity and have a good dechlorination capacity at low levels of energy consumption. The contribution of DIRB to the removal of pesticides, antibiotics and POPs is summarized. Then the DIRB electron transfer mechanism is discussed, which is core to their ability to dechlorinate. Finally, the prospect of future work on the removal of chlorine-containing organic pollutants by DIRB is presented, and the main challenges and further research directions are suggested.

Efficient low-concentration phosphate removal from sub-healthy surface water by adsorbent prepared based on functional complementary strategy.

The remediation of low-concentration phosphorus polluted surface water (LP-SW) is one of most challenging environmental issues worldwide. Adsorption is more suitable for LP-SW remediation due to its low cost and operability. Based on the strategy of functional complementation among industrial solid wastes (ISWs), ISW-based phosphate absorbent material (PAM) was prepared from coal ash (CA, binder), rich­calcium (Ca) carbide slag (CS, active component) and iron salt (functional reagent) by optimizing materials ratios and roasting conditions. PAM prepared under optimal conditions (Fe/CC-2opt) had good phosphate adsorption efficiency. Notably, Fe/CC-2opt not only ensured that the effluent met Environmental Quality Standards for Surface Water (pH = 6.0-9.0), but also facilitated the formation of brushite instead of hydroxyapatite due to FeSO4 addition. Compared with hydroxyapatite, brushite had greater potential application value as fertilizer due to its solubility and high P/Ca ratio. The possible mechanisms of phosphate adsorption by PAM included surface precipitation, surface complexation, electrostatic adsorption and release of Ca2+/OH-. Preparation cost of PAM was 80 US$/ton, and treatment cost was 0.07 US$/g P. Regeneration efficiency of PAM was still above 80 % after five cycles. The design idea and result of this study provide theoretical basis and technical support for the preparation of PAM with low cost, commercial production and great adsorption capacity.

Functional materials contributing to the removal of chlorinated hydrocarbons from soil and groundwater: Classification and intrinsic chemical-biological removal mechanisms.

Chlorinated hydrocarbons (CHs) are the main contaminants in soil and groundwater and have posed great challenge on the remediation of soil and ground water. Different remediation materials have been developed to deal with the environmental problems caused by CHs. Remediation materials can be classified into three main categories according to the corresponding technologies: adsorption materials, chemical reduction materials and bioaugmentation materials. In this paper, the classification and preparation of the three materials are briefly described in terms of synthesis and properties according to the different types. Then, a detailed review of the remediation mechanisms and applications of the different materials in soil and groundwater remediation is presented in relation to the various properties of the materials and the different challenges encountered in laboratory research or in the environmental application. The removal trends in different environments were found to be largely similar, which means that composite materials tend to be more effective in removing CHs in actual remediation. For instance, adsorbents were found to be effective when combined with other materials, due to the ability to take advantage of the respective strengths of both materials. The rapid removal of CHs while minimizing the impact of CHs on another material and the material itself on the environment. Finally, suggestions for the next research directions are given in conjunction with this paper.

Upgrading the peroxi-coagulation treatment of complex water matrices using a magnetically assembled mZVI/DSA anode: Insights into the importance of ClO radical.

The electrochemical technologies for water treatment have flourished over the last decades. However, it is still challenging to treat the actual complex water effluents by a single electrochemical process, often requiring coupling of technologies. In this study, an upgraded peroxi-coagulation (PC) process with a magnetically assembled mZVI/DSA anode has been devised for the first time. COD, NH3-N and total phosphorous were simultaneously and effectively removed from livestock wastewater. The advantages, influence of key parameters and evolution of electrogenerated species were systematically investigated to fully understand this novel PC process. The fluorescent substances in livestock wastewater could also be almost removed under optimal conditions (300 mA, 0.2 g ZVI particles and pH 6.8). The interaction between OH and active chlorine yielded ClO with a high steady-state concentration of 6.85 × 10-13 M, which did not cause COD removal but accelerated the oxidation of NH3-N. The Mulliken population suggested that OH and NH3-N had similar electron-donor behavior, whereas ClO acted as an electron-withdrawing species. Besides, although the energy barrier for the reaction between OH and NH3-N (17.0 kcal/mol) was lower than that with ClO (18.8 kcal/mol), considering the tunneling in the H abstraction reaction, the Skodje-Truhlar method adopted for calculations evidenced a 17-fold faster NH3-N oxidation rate with ClO. In summary, this work describes an advantageous single electrochemical process for the effective treatment of a complex water matrix.

Biochar-supported nZVI for the removal of Cr(VI) from soil and water: Advances in experimental research and engineering applications.

Over the past decade, biochar-supported nZVI composites (nZVI/biochar) have been developed and applied to treat various pollutants due to their excellent physical and chemical properties, especially in the field of chromium (VI) removal. This paper reviewed the factors influencing the preparation and experiments of nZVI/biochar composites, optimization methods, column experimental studies and the mechanism of Cr(VI) removal. The results showed that the difference in raw materials and preparation temperature led to the difference in functional groups and electron transfer capabilities of nZVI/biochar materials. In the experimental process, pH and test temperature can affect the surface chemical properties of materials and involve the electron transfer efficiency. Elemental doping and microbial coupling can effectively improve the performance of nZVI/biochar composites. In conclusion, biochar can stabilize nZVI and enhance electron transfer in nZVI/biochar materials, enabling the composite materials to remove Cr(VI) efficiently. The study of column experiments provides a theoretical basis for applying nZVI/biochar composites in engineering. Finally, the future work prospects of nZVI/biochar composites for heavy metal removal are introduced, and the main challenges and further research directions are proposed.

Recent advances and trends of trichloroethylene biodegradation: A critical review.

Trichloroethylene (TCE) is a ubiquitous chlorinated aliphatic hydrocarbon (CAH) in the environment, which is a Group 1 carcinogen with negative impacts on human health and ecosystems. Based on a series of recent advances, the environmental behavior and biodegradation process on TCE biodegradation need to be reviewed systematically. Four main biodegradation processes leading to TCE biodegradation by isolated bacteria and mixed cultures are anaerobic reductive dechlorination, anaerobic cometabolic reductive dichlorination, aerobic co-metabolism, and aerobic direct oxidation. More attention has been paid to the aerobic co-metabolism of TCE. Laboratory and field studies have demonstrated that bacterial isolates or mixed cultures containing Dehalococcoides or Dehalogenimonas can catalyze reductive dechlorination of TCE to ethene. The mechanisms, pathways, and enzymes of TCE biodegradation were reviewed, and the factors affecting the biodegradation process were discussed. Besides, the research progress on material-mediated enhanced biodegradation technologies of TCE through the combination of zero-valent iron (ZVI) or biochar with microorganisms was introduced. Furthermore, we reviewed the current research on TCE biodegradation in field applications, and finally provided the development prospects of TCE biodegradation based on the existing challenges. We hope that this review will provide guidance and specific recommendations for future studies on CAHs biodegradation in laboratory and field applications.

Comparative effects of environmental factors on bacterial communities in two types of indoor dust: Potential risks to university students.

University students are constantly exposed to potential bacterial pathogens and environmental pollutants in indoor environment because they spend most of their time indoors. University dormitory and printing shop are two typical indoor environments frequented by university students. However, little is known about the characteristics of bacterial community as well as the effect of indoor environmental factors on them. 16S rRNA gene sequencing was used to reveal the bacterial community in indoor dust, electronic devices were recorded during dust sampling, and polybrominated diphenyl ethers (PBDEs) were detected by gas chromatography mass spectrometry (GC-MS). Proteobacteria, Actinobacteria and Firmicutes were leading phyla, and Acinetobacter, Paracoccus and Kocuria were dominating genera. The predominant genera showed Acinetobacter > Paracoccus > unidentified Corynebacteriaceae in indoor dusts from university dormitories, whereas Paracoccus > unidentified Cyanobacteria > Acinetobacter in printing shops. The occurrence of Acinetobacter, Kocuria, Corynebacterium, Pseudomonas, and Bacillus suggested the health risks of potential pathogenic bacteria to university students. Significant differences of microbial composition and diversity were proved between university dormitories and printing shops. Chemoheterotrophy and aerobic chemoheterotrophy were dominant bacterial functions, and the seven primary bacterial functions displayed university dormitory > printing shop. BDE 138 and BDE 66 were main environmental parameters affecting the indoor dust bacterial community in university dormitory, while printer and BDE 47 played dominating role in shaping microorganism in printing shop. The complex biotic (potential bacterial pathogens) and abiotic factors (electronic equipment and chemical pollutants) in indoor dusts may pose potential health risks to university students.

A novel fate and transport model for evaluating the presence and environmental risk of per-/poly-fluoroalkyl substances (PFASs) among multi-media in Lingang hybrid constructed wetland, Tianjin, China.

Accurately revealing and predicting the presence and risks of per-/poly-fluoroalkyl substances (PFASs) in constructed wetlands (CWs) is great significant for the construction and management of CWs, but very challenging. In this work, a novel fate and transport model was for the first time established to evaluate the spatially continuous distribution and environmental risks of PFASs among multi-media in Lingang hybrid CW fed by industry tailwater. 20 PFASs were detected from the Lingang CW, and the total concentration of the detected PFASs in water and sediments were in the range of 38.94-81.65 ng/L and 1.23-4.31 ng/g, respectively. PFOA, PFOS and PFBS were the main pollutants in water and sediments. A fate and transport model describing the distribution characteristics and fate of PFASs in Lingang hybrid CW was constructed, and its reliability was verified. The simulated results suggested that PFASs were mainly accumulated in sediments and long-chain PFASs were more easily adsorbed by sediments compared with short-chain PFASs. According to the principal component analysis-multiple linear regression (PCA-MLR), PFASs mainly came from the tailwater from the surrounding sewage treatment plants. Besides, the environmental risks were predicted by this novel model, suggesting that the risks still cannot be neglected due to the accumulation and continuous input of PFASs although the environmental risks of Lingang CW were low. This work provides a novel model for the understanding of presence and risks of PFASs among multi-media in CWs.

Graphene oxide promoted chromium uptake by zebrafish embryos with multiple effects: Adsorption, bioenergetic flux and metabolism.

The increasing production and application of graphene oxide (GO, a popular carbon nanomaterial), makes their release into aqueous environment inevitably. The capability of GO to enhance the toxicity of background contaminants has been widely concerned. However, the effect of GO on heavy metal accumulation in fish embryos remains unclear. Here, we show that GO-promoted chromium (Cr) uptake by zebrafish embryos with multiple effects. The adsorption accelerated the aggregation and settlement of Cr6+-adsorbed GO and decreased the Cr6+ concentration in the upper water, which enhanced the interaction of chorions and contaminants (Cr6+, GO and Cr6+-adsorbed GO). In the presence of GO, the Cr content in chorions and intra-chorion embryos was increased by four times and 57% respectively, compared to that of the single Cr6+ exposure. Furthermore, GO+Cr6+ increased the oxygen consumption rates, embryonic acid extrusion rates and ATP production, induced more serious oxidative stress, and disturbed amino acid metabolism, fatty acid metabolism and TCA cycle. These findings provide new insights into the effect of GO on heavy metal bioaccumulation and toxicity during embryogenesis.

Providing a view for toxicity mechanism of tetracycline by analysis of the connections between metabolites and biologic endpoints of wheat.

Metabolomics is an implement for testing the toxicity of antibiotics, and provides a comprehensive view of the overall response to stress; however, the connections between metabolites and biologic endpoints keep unclear in response to antibiotics. In this study, wheat seeds were exposed to tetracycline for 5 days. The results proved that tetracycline restrained growth, reduced chlorophyl and carotinoid contents and cell permeability, and increased reactive oxygen species (ROS) levels and malondialdehyde (MDA) content. Orthogonal partial least squares (OPLS) was used to analyze the connections between metabolites and biologic endpoints, which discovered that 11 metabolic pathways were significantly affected by tetracycline, and amino acid metabolism could largely apply to root growth and ROS accumulation, while carbohydrate metabolism could have a ruling effect on tetracycline-induced cell permeability. 13 metabolites all played active roles in mediating tetracycline's effects on root length, root fresh weight and cell permeability but had no significant effects on ROS levels. The majority of metabolites with passive effects on root length, root fresh weight and cell permeability had active effects on ROS levels. These results offer a view about stress reaction of wheat to tetracycline.

Acute effects of ambient air pollution on outpatients with chronic rhinitis in Xinxiang, China.

Air pollution exposure leads to increased mortality and morbidity rates of respiratory diseases. Most of the evidence was founded on acute diseases such as acute lower respiratory diseases. However, limited studies have been conducted to evaluate the effects of air pollution on chronic respiratory diseases. This time-series study was conducted to examine the acute effects of 6 criteria ambient air pollutants on hospital outpatients with chronic rhinitis (CR) in Xinxiang, China. We retrieved 223,826 outpatient records of patients with respiratory diseases, of which 62,901 were those of patients with CR. Results showed that the current 10-µg/m3 increase in fine particulate matter (PM2.5), inhalable particulate matter (PM10), sulfur dioxide (SO2), nitrogen dioxide (NO2), and carbon monoxide (CO) corresponds to 0.67% (95% confidence interval [CI]: 0.15-1.18%), 0.58% (95% CI: 0.24-0.92%), 1.89% (95% CI: 0.52-3.27%), 3.01% (95% CI: 1.66-4.35%), and 0.06% (95% CI: 0.03-0.10%) increments in outpatients with CR, respectively. In addition, the effects in the male were stronger than those in the female. Higher effect estimates were observed in the old (≥ 65 years of age) and younger (< 15 years of age) groups. Our study confirmed the association between air pollution and outpatients with CR in Xinxiang, China. More stringent air pollution control measures must be implemented.

Acute effects of ambient air pollution on hospital outpatients with chronic pharyngitis in Xinxiang, China.

We present results on a time-series study that analyzed the acute effects of six criteria air pollutants on hospital outpatient with chronic pharyngitis (CP) in Xinxiang, China. Data on the concentration of air pollutants and CP outpatient records were collected daily in Xinxiang, China, from January 1, 2015 to December 31, 2018. This study identified 62,823 outpatients with CP. The annual average concentrations of PM2.5, PM10, SO2, NO2, CO, and O3 are 75.7, 132.1, 33.2, 48.4, 1377, and 59.4 µg/m3, respectively. Further, a 10 µg/m3 increment in the concentration of PM10, SO2, NO2, and CO corresponds to an increase of 0.28% (95% confidence interval (CI): 0.03-0.53%), 1.10% (95% CI: 0.09-2.11%), 1.82% (95% CI: 0.84-2.80%), and 0.03% (95% CI: 0.01-0.06%) in daily CP hospital outpatients, respectively. Furthermore, results indicated that outpatients under the age of 15 are more susceptible to the air pollutants, excluding O3. Meanwhile, males might be more susceptible, and effect estimates appear slightly stronger in the cool season. Therefore, we should implement effective measures to manage air pollutants and reinforce protection of the high-risk population.

Exposure pathways, levels and toxicity of polybrominated diphenyl ethers in humans: A review.

Polybrominated diphenyl ethers (PBDEs) are extensively used as brominated flame retardants (BFRs) in different types of materials, which have been listed as Persistent Organic Pollutants (POPs) by the Stockholm Convention in 2009 and 2017. Due to their ubiquities in the environment and toxicities, PBDEs have posed great threat to both human health and ecosystems. The aim of this review is to offer a comprehensive understanding of the exposure pathways, levels and trends and associated health risks of PBDEs in human body in a global scale. We systematically reviewed and described the scientific data of PBDE researches worldwide from 2010 to March 2020, focusing on the following three areas: (1) sources and human external exposure pathways of PBDEs; (2) PBDE levels and trends in humans; (3) human data of PBDEs toxicity. Dietary intake and dust ingestion are dominant human exposure pathways. PBDEs were widely detected in human samples, especially in human serum and human milk. Data showed that PBDEs are generally declining in human samples worldwide as a result of their phasing out. Due to the common use of PBDEs, their levels in humans from the USA were generally higher than that in other countries. High concentrations of PBDEs have been detected in humans from PBDE production regions and e-waste recycling sites. BDE-47, -153 and -99 were proved to be the primary congeners in humans. Human toxicity data demonstrated that PBDEs have extensively endocrine disruption effects, developmental effects, and carcinogenic effects among different populations.

Effects of tetracycline on growth, oxidative stress response, and metabolite pattern of ryegrass.

Tetracycline is an antibiotic that frequently contaminates the environment. In this study, the growth and metabolites of ryegrass seedlings treated with tetracycline (0, 1, 10 or 100 mg/L) for 5 days were investigated. The results showed that the growth of ryegrass and the concentrations of carotenoid and chlorophyll decreased as the tetracycline concentration increased. Tetracycline increased the production of reactive oxygen species (ROS) and cell permeability and triggered mitochondrial membrane potential loss in the roots of ryegrass. The metabolic profiles of ryegrass differed between the control and tetracycline-treated groups. The contents of glucose, shikimic acid, aconitic acid, serine, lactose, phenylalanine, mannitol, galactose, gluconic acid, asparagine, and glucopyranose were positively correlated with root length and had high variable importance projection values. These compounds may have crucial functions in root extension. Tetracycline also affected aminoacyl-tRNA biosynthesis, nitrogen metabolism, and alanine, aspartate and glutamate metabolism in the roots. Tetracycline may affect root extension by regulating the synthesis/degradation of these metabolites or the activity of their biosynthetic pathways. These results provide an insight into the stress response of ryegrass to tetracycline.

The occurrence of polybrominated diphenyl ether (PBDE) contamination in soil, water/sediment, and air.

As a kind of brominated flame retardants (BFRs), polybrominated diphenyl ethers (PBDEs) are extensively used in different types of electronic equipment, furniture, plastics, and textiles. PBDEs are ubiquitous environmental contaminants that may impact human health and ecosystems. Here we highlight recent findings on the occurrence, contamination status, and transport of PBDEs in soil, water/sediment, and air. Four aspects are discussed in detail: (1) sources of PBDEs to the environment; (2) occurrence and transport of PBDEs in soil; (3) PBDEs in aquatic ecosystems (water/sediment) and their water-sediment partitioning; and (4) the occurrence of PBDEs in the atmosphere and their gas-particle partitioning. Future prospects for the investigation on PBDEs occurrence are also discussed based on current scientific and practical needs.

Effects of soil properties, heavy metals, and PBDEs on microbial community of e-waste contaminated soil.

Heavy metals and polybrominated diphenyl ethers (PBDEs) are ubiquitous pollutants at electronic waste (e-waste) contaminated sites, their individual impacts on soil microbial community has attracted wide attention, however, limited research is available on the combined effects of heavy metals and PBDEs on microbial community of e-waste contaminated. Therefore, combined effects of heavy metals and PBDEs on the microbial community in the e-waste contaminated soil were investigated in this study. Samples were collected from Ziya e-waste recycling area in Tianjin, northern China, and the soil microbial communities were then analyzed by the high-throughput MiSeq 16S rRNA sequencing to assess the effects of soil properties, heavy metals, and PBDEs on the soil microbial community. Candidatus Nitrososphaera, Steroidobacter and Kaistobacter were the dominant microbial species in the soils. Similar microbial metabolic functions, including amino acid metabolism, carbohydrate metabolism and membrane transport, were found in all soil samples. Redundancy analysis and variation partition analysis revealed that the microbial community was mainly influenced by PBDEs (including BDE 183, BDE 99, BDE 100 and BDE 154) in horizontal soil samples. However, TN, biomass, BDE 100, BDE 99 and BDE 66 were the major drivers shaping the microbial community in vertical soil samples.

Occurrence and distribution of polybrominated diphenyl ethers in soils from an e-waste recycling area in northern China.

Polybrominated diphenyl ethers (PBDEs) are widespread persistent organic pollutants (POPs) because of their extensive use in diverse electronic products, which have posed great threats to human health and ecosystem. In this study, a total of 54 soil samples were collected from an e-waste recycling area in Tianjin, northern China for analyzing the occurrence and distribution of 14 PBDE congeners. The concentrations of BDE 209, ∑13PBDEs and ∑14PBDEs in the soils from Ziya e-waste recycling area were 2.9-2666 ng/g dw (dry weight) (average 90 ng/g dw), 3.0-41 ng/g dw (average 13 ng/g dw) and 5.9-2699 ng/g dw (average 103 ng/g dw), respectively. The ∑14PBDEs concentration showed a dramatic decrease from the central area to the surrounding area. Generally, PBDEs in the northern part showed higher levels than the southern part of the e-waste recycling area due to the wind direction in Tianjin. Deep soil was less polluted by PBDEs, which largely comes from the deposition, migration and infiltration of PBDEs in the surface soils. Overall, PBDEs level in the studied area was much lower than some typical e-waste recycling areas in south China, such as Guiyu and Qingyuan, but significantly higher than the non-e-waste recycling areas. BDE 209, BDE 138 and BDE 28 were the three dominant PBDE congeners in the soil. Principal component analysis (PCA) indicated that the commercial penta-BDEs and deca-BDE could be considered as the main sources of PBDEs pollution in this region. Redundancy analysis (RDA) suggested that the local PBDEs sources rather than soil properties influenced the PBDEs distribution in Ziya e-waste recycling area. This study systematically revealed the occurrence and distribution of PBDEs in soils from the biggest established circular economy park in northern China.

Biodegradation of decabromodiphenyl ether (BDE 209) by a newly isolated bacterium from an e-waste recycling area.

Polybrominated diphenyl ethers (PBDEs) have become widespread environmental pollutants all over the world. A newly isolated bacterium from an e-waste recycling area, Stenotrophomonas sp. strain WZN-1, can degrade decabromodiphenyl ether (BDE 209) effectively under aerobic conditions. Orthogonal test results showed that the optimum conditions for BDE 209 biodegradation were pH 5, 25 °C, 0.5% salinity, 150 mL minimal salt medium volume. Under the optimized condition, strain WZN-1 could degrade 55.15% of 65 µg/L BDE 209 under aerobic condition within 30 day incubation. Moreover, BDE 209 degradation kinetics was fitted to a first-order kinetics model. The biodegradation mechanism of BDE 209 by strain WZN-1 were supposed to be three possible metabolic pathways: debromination, hydroxylation, and ring opening processes. Four BDE 209 degradation genes, including one hydrolase, one dioxygenase and two dehalogenases, were identified based on the complete genome sequencing of strain WZN-1. The real-time qPCR demonstrated that the expression level of four identified genes were significantly induced by BDE 209, and they played an important role in the degradation process. This study is the first to demonstrate that the newly isolated Stenotrophomonas strain has an efficient BDE 209 degradation ability and would provide new insights for the microbial degradation of PBDEs.

Complete Genome Sequence of Stenotrophomonas sp. Strain WZN-1, Which Is Capable of Degrading Polybrominated Diphenyl Ethers.

Stenotrophomonas sp. strain WZN-1, isolated from an e-waste recycling area in Tianjin, China, is capable of degrading polybrominated diphenyl ethers (PBDEs). The complete genome of strain WZN-1 consists of 4,512,703 bp. This genome information will provide important information about the biodegradation pathways and mechanisms of PBDEs.

Photochlorination-induced transformation of graphene oxide: Mechanism and environmental fate.

With the increasing production and wide utilization of graphene oxide (GO), the nanomaterials are expected to be released into the environment, and end up in surface waters, and/or wastewater treatment plants. This study explored the changes in the physicochemical properties of GO resulting from photochlorination- simulating the reactions that occur during water and wastewater treatment. Photochlorination resulted in significant changes in the surface oxygen-functionalities of the nanomaterials, and fragmenting of the graphenic carbon sheets was observed. We found that photochlorination can enhance the decomposition of GO through the formation of reduced GO. The changes in surface oxygen-functionalities of GO were attributed to the oxidation by chlorine of the nanomaterials' quinone groups, and further oxidation by and/or radicals. The surface charge of GO, measured by its zeta potential, increased in magnitude with chlorination but decreased in magnitude with photochlorination, leading to the decrease in the colloidal stability of the photochlorinated nanomaterials. The antibacterial effect of the nanomaterials increased with both chlorination and photochlorination. This study clearly shows how the physicochemical properties, and environmental fate and effect of GO are modified by photochlorination.