Serological evidence of exposure to Toxoplasma gondii infection in wild birds and local chickens (Gallus gallus domesticus) in Plateau State, North Central Nigeria.

Toxoplasma gondii, the cause of toxoplasmosis is an obligate intracellular protozoan parasite of veterinary and public health importance. Ground feeding birds play an active role in the epidemiology of the disease and are the best indicators of soil contamination with the oocysts. Thirteen (14.1%; 95% CI = 7.7-23.0) of the 92 wild birds (6 of Coliformes; 4 of Columbiformes; 67 of Passeriformes and 15 of Piciformes) captured from Shere hills and 43 (9.3%; 95%CI = 4.9-12.4) of the 460 local chickens obtained across 3 senatorial regions of Plateau State, Nigeria were positive for anti-T. gondii antibodies using enzyme linked immunosorbent assay (ELISA). Varying prevalence (16.7-100%) of anti-T. gondii antibodies were recorded across 10 of 35 wild bird species; 2 (28.6) of 7 African thrush (Turdus pelios), 2 (33.3) of 6 speckled mouse bird (Colius striatus), 1 (20.0%) of 5 speckled fronted weaver (Sporopipes frontalis), 1 (33.3%) of 3 Northern Red bishop (Euplectes franciscanus), 1 (50.0%) of 2 village weaver (Ploceus cucullatus), 2 (28.6%) of 7 common bulbul (Pycnonotus barbatus), 1 (50.0%) of 2 black crowned tchagra (Tchagra senegalus), 1 (50.0%) of 2 black billed wood dove (Turtur abyssinicus), 1 (100.0%) of 1 mocking cliff chat (Thamnolaea cinamomeiventris), 1 (16.7) of 6 vieillot's barbet (Lybius vieilloti). The prevalence did not vary significantly based on the sex (p = 0.14), feeding guild (p = 0.53) and the taxonomic order (p = 0.62) of the wild birds. Regional based prevalence among local chickens ranged between 8.0 and 11.8% across the 3 senatorial zones. The source (p = 0.513; 95% CI = 4.9-16.8) and age (p = 0.797; 95% CI = 4.9-16.8) of local chickens were not associated with T. gondii seropositivity. Seropositivity was higher in the females (95% CI = 9.1-18.3) than in the males (p = 0.004; 95% CI = 3.0-9.4). This is the first report of the serological evidence of exposure to T. gondii infection in a wide variety of wild birds and local chickens in the area. It suggest that birds in this region are important asymptomatic intermediate hosts and play a role in the maintenance of the organism in the ecosystem.

Characterization of Foot-and-Mouth Disease Viruses Collected in Nigeria Between 2007 and 2014: Evidence for Epidemiological Links Between West and East Africa.

This study describes the molecular characterization of 47 foot-and-mouth disease (FMD) viruses recovered from field outbreaks in Nigeria between 2007 and 2014. Antigen ELISA of viral isolates was used to identify FMD virus serotypes O, A and SAT 2. Phylogenetic analyses of VP1 nucleotide sequences provide evidence for the presence of multiple sublineages of serotype SAT 2, and O/EAST AFRICA 3 (EA-3) and O/WEST AFRICA topotypes in the country. In contrast, for serotype A, a single monophyletic cluster of viruses has persisted within Nigeria (2009-2013). These results demonstrate the close genetic relatedness of viruses in Nigeria to those from other African countries, including the first formal characterization of serotype O/EA-3 viruses in Nigeria. The introductions and persistence of certain viral lineages in Nigeria may reflect transmission patterns via nomadic pastoralism and animal trade. Continuous monitoring of field outbreaks is necessary to dissect the complexity of FMD epidemiology in sub-Saharan Africa.

Serological survey of bovine brucellosis in Fulani nomadic cattle breeds (Bos indicus) of North-central Nigeria: Potential risk factors and zoonotic implications.

A cross sectional study was conducted to investigate seroprevalence and associated risk factors of bovine brucellosis in Fulani nomadic herds in the 3 agro-ecological zones of Niger State, North-central Nigeria between January and August 2013. A total of 672 cattle in 113 herds were screened for Brucella antibodies using Rose Bengal Plate Test (RBPT) and confirmed by Lateral flow Assay (LFA). Data on herd characteristics and zoonotic factors were collected using structured questionnaire administered on Fulani herd owners. Factors associated with Brucella infection were tested using Chi-square test and multivariable logistic model. The overall cattle-level seroprevalence was 1.9% (95% CI: 1.1-3.2) with highest in agro-zone C (3.2%). Herd-level seroprevalence was 9.7% (95% CI: 5.23-16.29) and highest in agro-zone C (13.5%). Sex and agro-ecological zones were significantly (P<0.006 and P<0.01, respectively) associated with Brucella abortus seropositivity. Herd composition, abortion in herd, exchange of bulls for mating, introduction of new cattle, and socio-cultural practices were significantly associated with brucellosis occurrence. Inhalation of droplets from milk of infected cows, and drinking raw milk were less likely [OR 0.27; 95% CI: 0.09-0.82 and OR 0.27; 95% CI: 0.08-0.99, respectively] not to predisposed to brucellosis in humans. Eating infected raw meat, and contact with infected placenta were more likely [OR 7.49; 95% CI: 2.06-28.32 and OR 5.74; 95% CI: 1.78-18.47, respectively] to be risks for the disease in humans. These results highlighted the important risk factors for bovine brucellosis in Fulani herds. Thus, brucellosis control programs which take these factors into consideration will be beneficial.