RESUMEN
This study was aimed to explore the inhibitory effect of pumpkin protein (cucurmosin, CUS) on proliferation of RPMI8226 myeloma cells in vitro and its mechanism. Western blot was used to detect the expression level of Notch-1, Jagged-2, P-Akt and NF-KB in the myeloma cells treated by different concentrations of CUS. The results demonstrated that CUS could down-regulate the protein expression levels of Notch1, Jagged-2, P-Akt and NF-KB in the myeloma cells and with time-and concentration-dependent way, at the same time CUS could also decrease the expressions of BCL-2 and P-Akt. It is concluded that CUS can obviously inhibit the RPMI8226 cell proliferation in vitro, down-regulate the expression levels of Notch signal and its down-stream target genes. Therefore, Notch signaling pathway can be used as a new treatment target for multiple myeloma, and CUS may be become a potential new drug for regulating Notch signaling pathway.
Asunto(s)
Mieloma Múltiple/metabolismo , Proteínas de Plantas/farmacología , Receptores Notch/metabolismo , Transducción de Señal/efectos de los fármacos , Línea Celular Tumoral , Humanos , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Proteína Jagged-2 , Proteínas de la Membrana/metabolismo , FN-kappa B/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Receptor Notch1/metabolismoRESUMEN
This study was aimed to investigate the antitumor effect of pumpkin protein (cucurmosin, CUS) on subcutaneous transplant tumor in chronic myeloid leukemia K562 cell-NOD/SCID mice and leukemia model. The subcutaneous transplant tumor in K562-NOD/SCID mice and leukemia model were established; using two models, the antitumor activity of CUS in mice was evaluated. The results indicated that the inhibitory rate of 0.5 mg/kg and 1 mg/kg CUS on subcutaneous transplant tumor were 53.45% and 59.43% respectively; survival time of mice received 0.25 mg/kg and 0.5 mg/kg CUS was 39.8 ± 5.5 d and 43.4 ± 6.6 d, antitumor rate was 24.9% and 36% respectively. It is concluded that CUS has significant inhibitory effect on mice with CML cell line K562.
Asunto(s)
Leucemia Mielógena Crónica BCR-ABL Positiva/patología , Proteínas de Plantas/farmacología , Animales , Femenino , Humanos , Células K562 , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Ratones , Ratones Endogámicos NOD , Ratones SCID , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
OBJECTIVE: To study the chemical constituents of Vitex trifolia. METHODS: Chromatography and spectroscopic analysis were employed to isolate and elucidate the chemical constituents in the plant. RESULTS: Five triterpenoids were obtained and identified as ursolic acid (I),2alpha,3alpha-dihydroxyurs-12-en-28-oic acid (II), betulinic acid (11), taraxerol (IV), 2alpha,3beta, 19-trihydroxyurs-12-en-28-oic acid (V). CONCLUSION: Compounds 1-5 are isolated from this plant for the first time.
Asunto(s)
Plantas Medicinales/química , Triterpenos/aislamiento & purificación , Vitex/química , Etanol/química , Estructura Molecular , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Ácido Oleanólico/aislamiento & purificación , Triterpenos Pentacíclicos , Espectrofotometría Infrarroja , Espectrofotometría Ultravioleta , Triterpenos/química , Ácido Betulínico , Ácido UrsólicoRESUMEN
OBJECTIVE: To study the chemical constituents of the root of Rhaponticum uniflorum. METHOD: Separation and purification were performed on silica gel and Sephadex LH-20 column chromatography. Their structure were elucidated on the basis of physicochemical and spectral analysis. RESULT: Five triterpenoid compounds were isolated and identified as ursolic acid (1), 3-oxo-19alpha-hydroxyurs-12-en-28-oic acid (2), pomolic acid (3), arjunic acid (4) and tormentic acid (5), respectively. CONCLUSION: Compounds 1 approximately 5 were isolated from the genus Rhaponticum for the first time.