Fanconi Anemia Complementary Group A (FANCA) Facilitates the Occurrence and Progression of Liver Hepatocellular Carcinoma.

BACKGROUND: Liver hepatocellular carcinoma (LIHC) is a serious liver disease worldwide, and its pathogenesis is complicated. AIMS: This study investigated the potential role of FANCA in the advancement and prognosis of LIHC. METHODS: Public databases, quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blot (WB) and immunohistochemistry (IHC) were employed to measure FANCA expression between tumor and normal samples. The relationship between FANCA expression and prognosis of LIHC patients were examined. Functional enrichment of FANCA-related genes was performed. Furthermore, univariate and multivariate analyses were conducted to determine the independent prognosis value of FANCA in LIHC. Finally, influence of FANCA knockout on the proliferation, migration, and invasion of HepG2 cell was validated with cloning formation, CCK8, and Transwell assays. RESULTS: Expression analysis presented that FANCA had high expression level in LIHC tissues and cells. Receiver operating characteristic (ROC) curve analysis showed that FANCA was of great diagnosis value in LIHC. Clinicopathological analysis revealed that FANCA was significantly greater expressed in the advanced stage than in the early stage of LIHC. Univariate, multivariate, and Kaplan-Meier survival analysis confirmed that high expression of FANCA was strongly associated with poor survival of LIHC patients. In addition, high level of FANCA in LIHC showed a negative association with immunoinfiltrated B cells, T cells, and stromal scores. Moreover, Knockout of FANCA significantly inhibited HepG2 cell proliferative activity, migration, and invasion ability. CONCLUSIONS: Our data revealed that high level of FANCA was closely associated with LIHC malignant progression, suggesting its potential utility as a diagnostic, predictive indicator, and therapeutic target.

Machine learning algorithms for a novel cuproptosis-related gene signature of diagnostic and immune infiltration in endometriosis.

Endometriosis (EMT) is an aggressive disease of the reproductive system, also called "benign cancer". However, effective treatments for EMT are still lacking in clinical practice. Interestingly, immune infiltration is significantly involved in EMT pathogenesis. Currently, no studies have shown the involvement of cuproptosis-related genes (CRGs) in regulating immune infiltration in EMT. This study identified three CRGs such as GLS, NFE2L2, and PDHA1, associated with EMT using machine learning algorithms. These three CRGs were upregulated in the endometrium of patients with moderate/severe EMT and downregulated in patients with infertility. Single sample genomic enrichment analysis (ssGSEA) revealed that these CRGs were closely correlated with autoimmune diseases such as systemic lupus erythematosus. Furthermore, these CRGs were correlated with immune cells such as eosinophils, natural killer cells, and macrophages. Therefore, profiling patients based on these genes aid in a more accurate diagnosis of EMT progression. The mRNA and protein expression levels of GLS, NFE2L2 and PDHA1 were validated by qRT-PCR and WB studies in EMT samples. These findings provide a new idea for the pathology and treatment of endometriosis, suggesting that CRGs such as GLS, NFE2L2 and PDHA1 may play a key role in the occurrence and development of endometriosis.

Exploring the diagnostic value, prognostic value, and biological functions of NPC gene family members in hepatocellular carcinoma based on a multi-omics analysis.

Liver cancer is a cunning malignancy with a high incidence and mortality rate among cancers worldwide. The NPC gene family members (NPCs: NPC1, NPC2, and NPC1L1) are closely linked to the development of multiple cancers, but their role in liver cancer remains unclear. As a result, we must investigate their functions in liver hepatocellular carcinoma (LIHC). NPCs were significantly differentially expressed between normal and LIHC tissues, with a high mutation frequency in LIHC. The ROC curve analysis revealed that NPC1/NPC2 had high diagnostic and prognostic values in LIHC. NPC1 expression was also found to be negatively correlated with its methylation level. The differentially expressed genes between high and low NPC1 expression groups in LIHC were mainly related to channel activity, transporter complexes, and plasma membrane adhesion molecules. Additionally, NPC1 expression was significantly associated with multiple immune cells and immunization checkpoints. It was hypothesized that a TUG1/SNHG4-miR-148a-3p-NPC1 regulatory axis is associated with hepatocarcinogenesis. Finally, the protein expression of NPC1 in LIHC tissues and paraneoplastic tissues was detected, and NPC1-knockdown HepG2 cells (NPC1KO) inhibited the proliferation, migration, and invasion. This study helped to identify new prognostic markers and potential immunotherapeutic targets for LIHC and revealed the molecular mechanisms underlying NPC1 regulation in LIHC. The NPCs play a key role in the prognosis and diagnosis of LIHC and may be an important indicator for LIHC prognosis and diagnosis; NPC1 might be a potential therapeutic target in LIHC.

A novel fluorescence probe for the recognition of Cd2+ and its application.

A facile fluorescence probe BQBH was synthesized and investigated on its spectrum property. The result showed that the BQBH had high sensitivity and selectivity for Cd2+ with lowest detection determined as 0.14 µM by fluorescence response. The 1: 1 binding ratio between BQBH and Cd2+ was determined by Job's plot, and the binding details were further confirmed by 1H NMR titration, FT-IR spectrum and HRMS analysis. The applications including on test paper, smart phone and cell image were all also investigated.

A novel dual-function probe for fluorescent turn-on recognition and differentiation of Al3+ and Ga3+ and its application.

In this study, a novel dual-function probe BMP based on benzothiazole was easily synthesized and characterized through common optical technique. In the system consisting of DMF/H2O (v/v, 2/3), probe BMP showed azure and blue-green to Al3+ and Ga3+, respectively. Besides, the binding ratios of BMP to Al3+ and Ga3+ were determined as 1:1, which confirmed by Job's plot. Furthermore, for Al3+ and Ga3+, the limit of detection (LOD) was determined to be 1.51 × 10-6 M and 4.28 × 10-6 M, respectively. Moreover, it was worth noting that BMP showed good performances in paper colorimetry, cell phone colorimetric identification and cell imaging.

Design of five two-dimensional Co-metal-organic frameworks for oxygen evolution reaction and dye degradation properties.

Two-dimensional (2D) metal-organic frameworks (MOFs) have been extensively investigated as oxygen evolution reaction (OER) materials because of their numerous advantages such as large specific surface areas, ultrathin thicknesses, well-defined active metal centers, and adjustable pore structures. Five Co-metal-organic frameworks, namely, [Co(L) (4.4'-bbidpe)H2O]n [YMUN 1 (YMUN for Youjiang Medical University for Nationalities)], {[Co2(L)2 (4.4'-bbibp)2]·[Co3(L) (4.4'-bbibp)]·DMAC}n (YMUN 2), [Co(L) (3,5-bip)]n (YMUN 3), [Co(L) (1,4-bimb)]n (YMUN 4), and [Co(L) (4.4'-bidpe)H2O]n (YMUN 5), were designed and fabricated from flexible dicarboxylic acid 1,3-bis(4'-carboxylphenoxy)benzene (H2L) and rigid/flexible imidazole ligands. Their frameworks consist of two-dimensional lamellar networks with a number of differences in their details. Their frameworks are discussed and compared, and their oxygen evolution reaction electrochemical activities and photocatalysis dye degradation properties are investigated.

A Significant Fluorescence Turn-On Probe for the Recognition of Al3+ and Its Application.

An easy prepared probe, BHMMP, was designed and synthesized, which displayed a significant fluorescence enhancement (over 38-fold) and obvious color change in the recognition of Al3+. The binding ratio of probe BHMMP to Al3+ was determined as 1:1, according to Job plot. The binding mechanism was fully clarified by the experiments, such as FT-IR spectrum, ESI-MS analysis, and 1H NMR titration. A DFT study further confirmed the binding mode of BHMMP to Al3+. The limit of detection (LOD) for Al3+ was determined as low as 0.70 µM, based on the fluorescence titration of BHMMP. Moreover, the results from real sample experiments, including real water samples, test papers, and cell images, well-demonstrated that BHMMP was capable of sensing Al3+ in environmental and biological systems.

Detection and correlation analysis of shellfish pathogens in Dadeng Island, Xiamen.

Food poisoning is caused by pathogenic bacteria in water and aquatic products, especially bivalves (e.g., oysters, clams), which can bioaccumulate pathogenic bacteria. Polluted water and aquatic products thus pose a serious threat to human health and safety. In this study, the types of pathogenic bacteria in water samples and shellfish collected from the Dadeng offshore area in Xiamen were examined. We also analyzed the relationships between dominant pathogens and major climate and water quality parameters. Our objective was to provide reference data that may be used to help prevent bacterial infections and to improve aquatic food hygiene in Xiamen and its surrounding areas to safe levels, thus ensuring the health of Xiamen residents. We found that the main pathogenic bacteria were Vibrio and Bacillus, with the dominant pathogen being Vibrio parahaemolyticus. Physical and chemical indexes (water temperature, salinity, pH, dissolved oxygen, and turbidity) of water bodies and the 3-day accumulated rainfall were found to be important factors affecting the occurrence and abundance of V. parahaemolyticus.

A Facile Probe for Fluorescence Turn-on and Simultaneous Naked-Eyes Discrimination of H2S and biothiols (Cys and GSH) and Its Application.

Hydrogen sulfide and biothiol molecules such as Cys and GSH acted important roles in many physiological processes. To simultaneously detect and distinguish them was quite necessary by a suitable fluorescent probe. A novel chemosensor 4-(4-(benzo[d]thiazol-2-yl)-2-methoxyphenoxy)-7-nitrobenzo[c][1,2,5]oxadiazole (BMNO) was designed to detect H2S/Cys/GSH using the combination of nitrobenzofurazan (NBD) and benzothiazole fluorophores linked by a facile ether bond. The probe BMNO was developed for simultaneous identification of H2S, Cys and GSH. Noticeably, the color changes (from colorless to light purple, light orange and light yellow) of probe BMNO solutions for sensing H2S, Cys and GSH could be observed by naked eyes, respectively. The probe BMNO exhibited high selectivity and sensitivity for H2S, Cys and GSH showing distinct optical signal with detection limit as low as 0.15 µM, 0.03 µM and 0.14 µM, respectively. The sensing mechanism was clarified by spectrum analysis and some controlled experiments. In addition, these outstanding properties of probe BMNO enabled its practical applications in detection H2S in beer, and in cell imaging for Cys and GSH as well.

A dual-functional probe for sensing pH change and ratiometric detection of Cu2.

A series of benzothiazole-based compounds were synthesized and characterized. Among them, probe Z showed significant dual-functional performance which was capable of sensing pH change and Cu2+. Probe Z displayed fluorescent turn-on under alkaline conditions due to deprotonation of the hydroxyl group along with the obviously color change from colorless to mint green. Interestingly, it further achieved in ratiometric detection of Cu2+ through absorbance or fluorescence signals in strong alkaline condition. The limit of detection was calculated correspondingly as 0.37 µM and 1.35 µM, respectively. Especially, the combination of the XNOR and INHIBIT logic gates could be used to confirm that one medium was in neutrality or alkalinity condition. Moreover, Z was successfully used in real water samples and test paper for fast identification of Cu2+, respectively.

A novel dual-function probe for recognition of Zn2+ and Al3+ and its application in real samples.

A dual-function probe NAHH based on naphthalene was synthesized and characterized. Based on the combination effects derived from the inhabitation of photo-induced electron transfer (PET) and CN isomerization, probe NAHH achieved in the recognition of Zn2+ and Al3+ both through obvious fluorescence enhancement and color changes detected by naked eye, respectively. Probe NAHH showed high sensitivity with the limit of detection as low as 3.02 × 10-7 M for Zn2+ and 7.55 × 10-8 M for Al3+, indicated the capability of probe NAHH in trace detection for Zn2+ and Al3+. The binding ratio of NAHH with Zn2+ and Al3+ were all 1:1 determined by Job plot, and the corresponding association constant was calculated as 8.48 × 104 M-1 and 4.45 × 105 M-1, respectively. The mechanism was further confirmed by FT-IR, 1H NMR titration and ESI-MS analysis. Furthermore, probe NAHH was successfully applied in logic gate construction and the detection of Zn2+ and Al3+ in Songhua River and test stripe. Fluorescence imaging experiments confirmed that NAHH could be used to monitor Zn2+ in plant root.

A Benzothiazole-Based Fluorescent Probe for Ratiometric Detection of Al3+ and Its Application in Water Samples and Cell Imaging.

An easily prepared benzothiazole-based probe (BHM) was prepared and characterized by general spectra, including 1H NMR, 13C NMR, HRMS, and single-crystal X-ray diffraction. Based on the synergistic mechanism of the inhabitation of intramolecular charge transfer (ICT), the BHM displayed high selectivity and sensitivity for Al3+ in DMF/H2O (v/v, 1/1) through an obvious blue-shift in the fluorescent spectrum and significant color change detected by the naked eye, respectively. The binding ratio of BHM with Al3+ was 1:1, as determined by the Job plot, and the binding details were investigated using FT-IR, 1H NMR titration, and ESI-MS analysis. Furthermore, the BHM was successfully applied in the detection of Al3+ in the Songhua River and on a test stripe. Fluorescence imaging experiments confirmed that the BHM could be used to monitor Al3+ in human stromal cells (HSC).

A Naphthalimide-Based Fluorescence "Off-on-Off" Chemosensor for Relay Detection of Al3+ and ClO.

A novel Al3+ chemosensor NPA was designed and synthesized on basis of the mechanism of ICT and CHEF. Upon addition of Al3+, the probe NPA displayed a bright green fluorescence under UV radiation and visual color change from yellow to colorless. Spectrum titrations showed that NPA could be recognized as a fluorescent turn-on probe with 10-8 M detection level. The probe was successfully applied in real water sample and test paper. More important, NPA-Al3+ complex were used as a fluorescent turn-off probe for the detection of ClO- with the detection as low as 2.34 × 10-8 M. The performance of NPA to Al3+ and NPA-Al3+ complex to ClO- demonstrated that NPA could be served as a sensitive probe and exhibit INHIBIT logic gate behavior with Al3+ and ClO- as inputs.

A Highly Selective Fluorescence "Turn on" and Absorbance-Ratiometric Detection of Al3+ in Totally H2O and its Application in Test Paper.

A novel naphthalene based fluorescence probe NBDH was designed and synthesized. Probe NBDH exhibited highly selective and sensitive responses towards Al3+ in HEPES-NaOH buffer solution (pH = 7.4). In addition, the detection of NBDH to Al3+ could be achieved through dual channels embodied in significant fluorescent turn-on signal and ratiometric absorbance response. The stoichiometry ratio of NBDH-Al3+ was 1:1 by fluorescence job' plot and binging mechanism was further varified by the FT-IR, NMR titration and HRMS. Furthermore, NBDH was achieved in real sample detection, and a series of color test paper were developed for visual detecting Al3+ ions.

A dual-function probe based on naphthalene for fluorescent turn-on recognition of Cu2+ and colorimetric detection of Fe3+ in neat H2O.

A simple naphthalene derivative, 6-hydroxy-2-naphthohydrazide (NAH), was designed and synthesized through two facile steps reactions with the 6-hydroxy-2-naphthoic acid (NCA) as the starting material. In neat H2O (10% 0.01 M HEPES buffer, v/v, pH = 7.4), probe NAH showed a highly selective and sensitive response towards Fe3+ via perceptible color change and displayed "turn-on" dual-emission fluorescence response for Cu2+. The binding stoichiometry ratio of NAH/Cu2+ and NAH/Fe3+ were all confirmed as 1:1 by the method of fluorescence job's plot and UV-Vis job's plot, respectively. Probe NAH can be used over a wide pH range for the determination of Fe3+ (2.0-10.0) and Cu2+ (6.0-10.0) without interference from other co-existing metal ions. A possible detection mechanism was the hydrolysis of NAH upon the addition of Fe3+ or Cu2+, thereby leading to the formation of 6-hydroxy-naphthalene-2-carboxylic acid (NCA) which was further confirmed by the various spectroscopic techniques including FT-IR, 1H NMR titration and HRMS. Moreover, NAH was successfully applied to the detection of Cu2+ and Fe3+ in tap water, ultrapure water and BSA.

A naphthalene-quinoline based chemosensor for fluorescent "turn-on" and absorbance-ratiometric detection of Al3+ and its application in cells imaging.

A new naphthalene-quinoline based chemosensor L was prepared and structurally characterized. L exhibited excellent selectivity and sensitivity to Al3+ through distinct fluorescence enhancement (335-fold) and ratiometric detection in DMF/H2O (v/v, 1/9) based on the combined mechanisms of ESIPT and CHEF. The recognizing behavior of L toward Al3+ had been investigated in detail through Job's Plot, FT-IR, HNMR, and HRMS analysis. The limit of detection (LOD) for Al3+ was as low as and 3.67 × 10-8 M. L was successfully applied in real sample detection and construction of molecular logic gate. Moreover, L was verified to be of low cytotoxicity and good imaging characteristics for the detection of Al3+ in cells HSC.

A Highly Selective Naphthalimide-Based Chemosensor: "Naked-Eye" Colorimetric and Fluorescent Turn-On Recognition of Al3+ and Its Application in Practical Samples, Test Paper and Logic Gate.

A novel naphthalimide-based colorimetric and fluorescent turn-on chemosensor for Al3+ was synthesized and characterized with spectroscopic techniques. In MeOH solution, BPAM showed high selectivity and sensitivity to Al3+ by a 60-fold fluorescence enhancement and blue-shift absorption with visible color changes attributed to the contribution of chelation enhanced fluorescence (CHEF) and inhibition of intramolecular charge transfer (ICT). A 1:1 BPAM-Al3+ complex confirmed by job's plot and HRMS with a binding constant of 6.37 × 104 M- 1, and the detection limit for Al3+ was as low as 1.59 × 10- 7 M. BPAM was successfully applied in real sample detection and assessing the existence of Al3+ by a colorimetric method on filter paper. Furthermore, the fluorescent signals of BPAM were designed to construct an INHIBIT molecular logic gate.

Synthesis and photocatalytic properties of visible-light-responsive, three-dimensional, flower-like La-TiO2/g-C3N4 heterojunction composites.

We prepared a new three-dimensional, flower-like La-TiO2/g-C3N4 (LaTiCN) heterojunction photocatalyst using a solvothermal method. Analysis and characterization were performed by conducting scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Fourier transform-infrared spectroscopy, ultraviolet-visible spectrophotometry, and nitrogen adsorption and desorption. The prepared g-C3N4 nanosheets could reach 100 nm in size and covered the TiO2 surface. A tightly bound interface formed between the g-C3N4 and TiO2, speeding up the effective transfer of photo-induced electrons. In addition, the incorporation of La3+ reduced the electron-hole recombination efficiency. Consequently, the prepared La-TiO2/g-C3N4 composite material exhibited better visible-light catalytic activity than pure TiO2.

A highly selective colorimetric and fluorescent turn-on chemosensor for Al(3+) based on naphthalimide derivative.

A new chemosensor L based on the naphthalimide moiety was synthesized and characterized. L exhibited the high selectivity and sensitivity for Al(3+) in CH3OH, along with colorimetric and fluorometric dual-signaling responses based on the joint contribution of the ICT and CHEF processes. A 1:1 stoichiometry for the L-Al(3+) complex was formed with an association constant of 7.6×10(4)M(-1), and the limit of detection for Al(3+) was determined as 6.9µM. In addition, L was successfully applied to the determination of Al(3+) in real water samples.

1-Dichloro-acetyl-8a-methyl-1,2,3,4,6,7,8,8a-octa-hydro-pyrrolo-[1,2-a]pyrimidin-6-one.

In the title compound, C(10)H(14)Cl(2)N(2)O(2), the five-membered ring adopts an envelope conformation (with the methylene C atom closest to the C-N bridge as the flap), while the conformation of the six-membered ring is close to a twist-boat. In the crystal, mol-ecules are linked by weak C-H⋯O hydrogen bonds, forming chains along the c-axis direction.