RESUMEN
OBJECTIVE: To optimize the prescription of baicalein long circulating liposomes (Baicalein LCLs). METHODS: The liposomes were prepared by the reverse evaporating method. The entrapment efficiency and the carrying amount were determined by HPLC-Sephadex method. The main influencing factors were identified by single factor analysis, then the techniques of the prescription and preparation of liposomes were optimized by orthogonal experiment. RESULTS: The best prescription was as follows: The proportionality of cholesterin to lecithin was 1 : 2; The dosage of PEG 4 000 was 2 mg; The proportionality of water phase to oil phase was 1 : 2; The dosage of baicalein was 30 mg. CONCLUSION: After optimized, the average entrapment efficiency of liposomes is 81.42% and the average carrying amount is 32.25%.
Asunto(s)
Portadores de Fármacos , Composición de Medicamentos/métodos , Flavanonas/administración & dosificación , Liposomas/química , Scutellaria baicalensis/química , Área Bajo la Curva , Colesterol/administración & dosificación , Cromatografía Líquida de Alta Presión , Estabilidad de Medicamentos , Flavanonas/química , Concentración de Iones de Hidrógeno , Lecitinas/administración & dosificación , Polietilenglicoles/administración & dosificaciónRESUMEN
OBJECTIVE: To study the chemical constituents of polyphenols in seed of Litchi chinensis. METHODS: The seeds of Litchi chinensis were extracted by 65% ethanol, then the compounds were separated by repeated silica gel, polyamide and preparative TLC. The structures of polyphones isolated were identified by analysis of their spectral datas and chemical properties. RESULTS: Four polyphones compounds were isolated as protocatechuic aldehyde (I), protocatechuic acid (II), daucosterol (III) and (-) -epicatichin (IV). CONCLUSIONS: Compounds I , III and IV are isolated from this plant for the first time.
Asunto(s)
Flavonoides/química , Litchi/química , Fenoles/química , Semillas/química , Sitoesteroles/química , Benzaldehídos/química , Benzaldehídos/aislamiento & purificación , Catequina/química , Catequina/aislamiento & purificación , Catecoles/química , Catecoles/aislamiento & purificación , Cromatografía en Capa Delgada/métodos , Etanol , Flavonoides/aislamiento & purificación , Hidroxibenzoatos/química , Hidroxibenzoatos/aislamiento & purificación , Estructura Molecular , Fenoles/aislamiento & purificación , Polifenoles , Sitoesteroles/aislamiento & purificaciónRESUMEN
OBJECTIVE: To investigate the process of separating and purifying total flavonoids from Smilax glabra. METHODS: With the contents of total flavonoids, astilbin and engelitin as indices, the optimum process of separating and purifying flavonoids from Smilax glabra was screened by static and dynamic adsorption tests. RESULTS: The static saturated adsorption capacity of polyamide resin to flavonoids of Smilax glabra was 144 mg/g (saturated in 2 h). The dynamic adsorption capacity was 128.125 mg/g, the optimum conditions of elution were as follows: the adsorbed resin was washed by 20BV water firstly, then by 20BV 95% ethanol. The content of obtained total flavonoids reached 71.84%, as the content of astilbin was 14.32%, and the content of engelitin was 1. 08%. CONCLUSIONS: The total flavonoids of Smilax glabra is able to be easily separated and purified by polyamide resin under the optimum conditions above.