Dynamics of ecosystem services and nonlinear responses to increased anthropogenic pressure.

Escalating global human activities elicit diverse ecosystem service responses, yet understanding remains limited. This study establishes a framework to clarify these responses, focusing on the Yangtze River Economic Belt in China. Analyzing 2000-2020 data, it calculates ecosystem service economic value and human footprint index. It introduces the ecosystem services response index and comprehensive responsiveness index to assess response characteristics and intensity to anthropogenic pressures. Results show a fluctuating decline in ecosystem services and an increase in anthropogenic pressures. There is a nonlinear relationship: ecosystem services decline with rising pressures, following a U-shaped trend. Notably, nonurban agglomerations experience more significant ecosystem service evolution than urban agglomerations due to differing environmental conditions. This highlights regional disparities in human activity impacts on ecosystems, crucial for planning.

Improving the Efficiency of Inferences From Hybrid Samples for Effective Health Surveillance Surveys: Comprehensive Review of Quantitative Methods.

BACKGROUND: Increasingly, survey researchers rely on hybrid samples to improve coverage and increase the number of respondents by combining independent samples. For instance, it is possible to combine 2 probability samples with one relying on telephone and another on mail. More commonly, however, researchers are now supplementing probability samples with those from online panels that are less costly. Setting aside ad hoc approaches that are void of rigor, traditionally, the method of composite estimation has been used to blend results from different sample surveys. This means individual point estimates from different surveys are pooled together, 1 estimate at a time. Given that for a typical study many estimates must be produced, this piecemeal approach is computationally burdensome and subject to the inferential limitations of the individual surveys that are used in this process. OBJECTIVE: In this paper, we will provide a comprehensive review of the traditional method of composite estimation. Subsequently, the method of composite weighting is introduced, which is significantly more efficient, both computationally and inferentially when pooling data from multiple surveys. With the growing interest in hybrid sampling alternatives, we hope to offer an accessible methodology for improving the efficiency of inferences from such sample surveys without sacrificing rigor. METHODS: Specifically, we will illustrate why the many ad hoc procedures for blending survey data from multiple surveys are void of scientific integrity and subject to misleading inferences. Moreover, we will demonstrate how the traditional approach of composite estimation fails to offer a pragmatic and scalable solution in practice. By relying on theoretical and empirical justifications, in contrast, we will show how our proposed methodology of composite weighting is both scientifically sound and inferentially and computationally superior to the old method of composite estimation. RESULTS: Using data from 3 large surveys that have relied on hybrid samples composed of probability-based and supplemental sample components from online panels, we illustrate that our proposed method of composite weighting is superior to the traditional method of composite estimation in 2 distinct ways. Computationally, it is vastly less demanding and hence more accessible for practitioners. Inferentially, it produces more efficient estimates with higher levels of external validity when pooling data from multiple surveys. CONCLUSIONS: The new realities of the digital age have brought about a number of resilient challenges for survey researchers, which in turn have exposed some of the inefficiencies associated with the traditional methods this community has relied upon for decades. The resilience of such challenges suggests that piecemeal approaches that may have limited applicability or restricted accessibility will prove to be inadequate and transient. It is from this perspective that our proposed method of composite weighting has aimed to introduce a durable and accessible solution for hybrid sample surveys.

Contradictions in human-nature relationships threaten coastal resilience and sustainability in the Bohai Rim Region, China.

Under the challenge of global environmental change and rapid development, tremendous risks brought about by natural disasters and human activities have increased environmental pressures for sustainable development. How to improve coastal resilience in the process of urban development has become an important topic in academia. In this study, a variable fuzzy recognition model was used to measure the level of coastal resilience in 17 cities in the Bohai Rim region, and then the kernel density, thiel index, and random forest model were used to explore the spatiotemporal characteristics and influencing factors of coastal resilience. The results show that (1) The overall resilience level of the Bohai Rim region is increasing over time, but at a relatively slow rate. (2) Coastal resilience has significant spatial unevenness, with high-level cities dominated by Tianjin, Qingdao, Yantai, etc. and low-level cities dominated by Cangzhou, Panjin, Yingkou, Binzhou, etc. (3) The influence of economic development, infrastructure, innovation ability, technology investment, and government regulation on coastal resilience decreases in order. Based on the research findings, the study can not only make suggestions for the actual regulation strategy but also provide empirical and theoretical experience for other coastal countries.

Identifying loss threshold and migration trajectory in the management of Suaeda salsa wetland under coastal squeeze.

The coastal salt marsh wetland is mainly located in the tidal area where sea and land are intertwined, which has the functions of promoting silt and consolidating beach, wave reduction and disaster reduction, and is an ecological barrier against erosion in the coastal zone, which has important ecological value. Nevertheless, owing to the intricate nature of wetland species formation mechanisms and community distribution patterns, the impact of coastal squeeze on Suaeda salsa wetlands loss may not exhibit a linear relationship, necessitating further elucidation of the specific processes involved. Precisely understanding the extent to which coastal squeeze affects the threshold of loss in the Suaeda salsa wetland holds immense importance in safeguarding the distinctive red beach landscape. Furthermore, it represents a pivotal scientific challenge that necessitates resolution in the management of coastal wetlands for their protection and restoration. Hence, drawing upon the theoretical framework concerning the impact of coastal squeeze on the degradation of coastal Suaeda salsa wetland, this study employs the representative silty coastal region of China's northern Liaodong Bay as a case study to conduct empirical investigation. The research integrates Landsat imagery and digital elevation data, and incorporates spatial autocorrelation, elastic coefficient analysis, and barycenter model analysis to examine the threshold of degradation and spatial variations of Suaeda salsa wetland in response to coastal squeeze. The results show that: (1) The risk intensity of coastal squeeze in the study area exhibited a pattern of initial decline followed by an increase from 1995 to 2020, with the highest risk areas predominantly located in the development zone on the eastern bank of the Liao River Estuary. (2) There was a positive correlation between the growth of the coastal squeeze index and the loss of the Suaeda salsa wetland. When the proportion of area in which the coastal squeeze deteriorates reached 43.9%, the Suaeda salsa wetland lose resilience against the impact of coastal squeeze, leading to an intensified rate of loss. (3) Compared with the west side of the Liao River, the critical area of coastal squeeze and the loss barycenter of the Suaeda salsa wetland on the east side of the Liao River have a greater range of changes. The planning and management of coastal ecological restoration necessitates considering the occurrence conditions of the coastal squeeze impact threshold on the loss threshold of wetland and the migration characteristics of the wetland loss area. This approach enables the timely control of coastal squeeze risk intensity in the area and the preservation of wetlands' resistance to external disturbances. Consequently, it holds immense importance for the sustainable development of coastal wetlands.

Spatiotemporal assessment of ecosystem services supply-demand relationships to identify ecological management zoning in coastal city Dalian, China.

Integrating ecosystem services supply-demand relationships into ecological management zoning is a hot topic. Most studies have focused on the matching relationship between the supply and demand of ecosystem services. However, the extent to which both are coordinated at different matching levels is ignored, that is, whether ecosystem services supply and demand tend to reinforce each other at high levels or constrain each other at low levels. Therefore, taking Dalian as an example, this study constructed a research framework for ecological management zoning by integrating the matching and coupling coordination relationship of ecosystem services supply-demand. We found that the supply of ecosystem services in Dalian decreased by 23.70% and the demand increased by 22.54% from 2005 to 2019. There was an obvious mismatch and disharmony in the supply and demand of ecosystem services, and the matching and coordination often did not exist simultaneously. Overlay analysis was used to divide Dalian into four ecological management zones: eco-conservation, eco-development, eco-improvement, and eco-restoration zones. This study helped in integrating the matching and coupling coordination relationship of ecosystem services supply-demand into the environmental management system, which has practical significance for the sustainable development of ecosystem services.

Late­onset immune checkpoint inhibitor­related pneumonitis after cessation of sintilimab: A case report and literature review.

Immune-related adverse events following treatment with immune checkpoint inhibitors (ICIs) can occur at any time during therapy, with onset occurring most frequently during the first 3 months of treatment. However, they rarely occur after treatment cessation. An awareness of delayed immune-related events following the termination of immunotherapy is paramount for optimal tumour management. The present study reports a case of a 69-year-old male patient with right lung adenocarcinoma. He suffered from psoriasis for ~40 years and was suspected of developing immune checkpoint inhibitor-related pneumonitis (CIP) 6 months after the cessation of treatment with the anti-programmed cell death-1 receptor antibody sintilimab. The present case study is, to the best of our knowledge, the first case of late-onset CIP after the cessation of sintilimab. Subsequently, the report also reviews previously reported cases of late-onset CIP after the cessation of ICI treatment. The present report highlights the finding that CIP can develop, although rarely reported, months or even years after the termination of immunotherapy. Therefore, CIP should always be considered as one of the possibilities and addressed accordingly once the pulmonary infection is ruled out. Careful monitoring, timely diagnosis and administration of corticosteroids are essential in controlling this condition, particularly for patients with pre-existing autoimmune diseases.

New insight into blue carbon stocks and natural-human drivers under reclamation history districts for sustainable coastal development: A case study from Liaohe River Delta, China.

Blue carbon is a vital aspect of climate change mitigation, which necessitates the identification of stocks and drivers for implementing mitigation strategies. However, reclamation may be among the most invasive forms, and the question of its influence has not been addressed well in blue carbon research. Therefore, the effects of reclamation on carbon stocks and the interaction of crucial drivers from reclamation time areas (1930s, 1960s, 1990s) were evaluated in the Liaohe River Delta (LRD) and compared with natural reserves (core, buffer, experimental areas). Carbon stocks based on InVEST model were lower than preexisting conditions (1.930 × 106 Mg-1.893 × 106 Mg). One-way Analysis of Variance showed that average carbon stocks accumulated 55 years after reclamation and reached the lowest value (13.19 Mg·ha-1) in 85 years. The interaction analysis of dominant drivers affecting carbon showed the difference between reclaimed areas and reserves regarding potential effect pathways. In the 1930s and 1960s reclamation time areas, crop yield and industrial output determined blue carbon by changing NO3--N and AP. In the 1990s reclamation time area, population density played an important role. In defining the impact of vegetation cover on carbon within the reserves, the distance to the coast and residence were significant factors. This study demonstrated that coastal management practices, such as the size of industry and population control and the balanced fertilization techniques in reclamation areas, maintaining adequate vegetation cover in reserve, played a crucial role in the improvement of blue carbon sinks.

Reduced Expression of RBP7 is Associated with Resistance to Tamoxifen In Luminal A Breast Cancer.

BACKGROUND: Tamoxifen is the most commonly used hormonal treatment for ERα-positive breast cancer. Tamoxifen resistance is still a big problem for ERα target therapy. RBP7 is a member of the cellular retinol-binding protein family. OBJECTIVE: This study aims to investigate the prognostic role of RBP7 and the relationship between RBP7 expression and sensitivity or resistance to tamoxifen in ERα-positive breast cancer. METHODS: A bioinformatics method was used to investigate RBP7 expression and the prognostic value of RBP7 in different subtypes of breast cancer. The relationship between RBP7 expression and sensitivity or resistance to tamoxifen was studied using clinical data (GSE1379) and cell line data (GSE27473, GSE2645923, GSM3715281, and GSM3715282). Transfection of RBP7 experiments was used to testify to the function of RBP7 in MCF7 cell. RESULTS: RBP7 is a member of the family of cellular retinol-binding proteins. RBP7 expression was down-regulated at both mRNA and protein levels in breast cancer and was not associated with different TNM (Tumor, Node, Metastasis) stages. High expression of RBP7 was significantly related to good relative percent survival in the luminal A subtype, but in negative breast cancer, the result was opposite. The ROC plot showed that RBP7 had a significant predictive value for the tamoxifen response in the luminal A subtype. The expression of RBP7 from patients with recurrence treated with tamoxifen was significantly reduced. Gene Expression Omnibus showed that RBP7 expression was reduced considerably in tamoxifen-resistant MCF7 cells and T47D cells. The expression of RBP7 was positively correlated with some microRNAs involved in negatively regulating tamoxifen-resistant breast cancer. We also found that the expression of RBP7 decreased significantly in tamoxifen-resistant MCF7 cells, and transfection of RBP7 increased the sensitivity of resistant cells to tamoxifen. CONCLUSION: Reduced expression of RBP7 is associated with resistance to tamoxifen in luminal A breast cancer. Our research may help to explore the mechanisms of resistance of breast cancer to tamoxifen.

CircPIM3 regulates taxol resistance in non-small cell lung cancer via miR-338-3p/TNFAIP8 axis.

Numerous work has revealed the involvement of circular RNA (circRNA) in regulating chemotherapy resistance. Here, we investigate circPIM3 role in taxol (Tax) resistance in non-small cell lung cancer (NSCLC). CircPIM3, microRNA (miR)-338-3p and tumor necrosis factor-alpha-induced protein-8 (TNFAIP8) expression were detected via quantitative real-time PCR, western blot or immunohistochemistry assay. Tax resistance was evaluated using cell counting kit-8, cell proliferation was measured by colony formation assay, cell cycle and apoptosis were examined via flow cytometry. The interplay between miR-338-3p and circPIM3 or TNFAIP8 was confirmed by dual-luciferase reporter assay. Finally, the effect of circPIM3 on Tax resistance in NSCLC in vivo was investigated by xenograft models. CircPIM3 and TNFAIP8 were upregulated in Tax-resistant NSCLC tissue and cell samples. Reducing circPIM3 expression inhibited Tax resistance, proliferation and induced cycle arrest and apoptosis in Tax-resistant NSCLC cells. Mechanically, circPIM3 absence led to downregulation of TNFAIP8 via absorbing miR-338-3p. Additionally, circPIM3 depletion increased Tax sensitivity of NSCLC in vivo. Silencing of circPIM3 suppressed Tax resistance in Tax-resistant NSCLC cells through regulation of the miR-338-3p/TNFAIP8 axis.

Integrating Spatial Heterogeneity into an Analysis between Ecosystem Service Value and Its Driving Factors: A Case Study of Dalian, China.

Human demand for natural resources has grown, leading to ecological debasement and related ecological system administration. Using Dalian as an example, we estimated the changes in the ecosystem service value (ESV) in 2005 and 2020. We used ArcGIS and spatial statistics to conduct estimations and change analyses of the ESV. Based on the results of the ESV, the geographical detector and geographically weighted regression (GWR) elucidated the contributions of different driving factors of the ESV in a 2 km grid. In summary, these results indicated that: (1) from a holistic perspective, the ESV of Dalian fell by 206.8009 billion CNY over 15 years, and the hot spots were concentrated in both the northern and the western parts, whereas the cold spots were distributed in the central part; (2) according to the results from the geographical detector, land use structure factors influenced the ESV most significantly, followed by socio-economic factors, and the impact of natural factors was relatively small; and (3) according to the results of the GWR, land use structure factors negatively affected the ESV, and the positive impact of the proportion of the natural land area was the most obvious. We conclude that the decline in the ESV reflects the impact of human activities on the ecosystem in the studied landscape. Understanding ESV changes should be made a priority in ecosystem management, and evaluating ESV drivers can contribute to developing land use strategies for policy-making.

Scales and Historical Evolution: Methods to Reveal the Relationships between Ecosystem Service Bundles and Socio-Ecological Drivers-A Case Study of Dalian City, China.

Ecosystem service (ES) bundles can be defined as the temporal and spatial co-occurrence of ESs. ES bundles are jointly driven by socio-ecological factors and form at different scales. However, in recent research, a few studies have analyzed the dynamic evolution and driving mechanisms of ES bundles at different scales. Therefore, this study explored the spatial patterns of six ESs supplied in Dalian (China) from 2005 to 2015 at three spatial scales, determining the distribution and evolution patterns of ES bundles and their responses to socio-ecological driving factors. Our results are as follows: (1) We identified four ES bundles representing ecological conservation, water conservation, ecological depletion, and food supply. The developmental trajectory of each ES bundle could be attributed to the combined effects of environmental conditions and urban expansion. In particular, the water conservation bundle and food supply bundle were changed to the ecological depletion bundle. Given the ongoing urbanization, the conflict between ESs has intensified. (2) The impact of socio-ecological driving factors on ES bundles vary with scale. At three spatial scales, the digital elevation model (DEM) and normalized difference vegetation index (NDVI) had a great impact on ES bundles. Urbanization indicators also strongly explain the spatial distribution of ES bundles at the county and grid scales. The interaction factor detector shows that there is no combination of mutual weakening, indicating that the formation of ES bundles is driven by multiple factors in Dalian. Overall, this study used a more holistic approach to manage the ecosystem by studying the temporal-spatial dynamics of the multiple ESs.

Effect of Metformin Therapy on Biological Properties and Prognosis of Breast Cancer Patients Complicated with Type-2 Diabetes.

Objectives: To evaluate the effect of Metformin therapy on patients of breast cancer with complications of Type-2 diabetes. Methods: Altogether 102 cases of breast cancer complicated with Type-2 diabetes admitted into Hebei General Hospital from January 2019 to December 2020 were included in the study. They were divided into two groups per whether Metformin was administered in the regimen, namely Metformin group and non-Metformin group. In the meanwhile, 106 cases of breast cancer without Type-2 diabetes admitted in the same period were selected to form a control group. Three groups were compared in terms of general data (incl. age, body mass, family history, menopause or not), clinical staging, tumor histological differentiation, molecular subtyping (Incl. Luminal A, Luminal B, ERBB2+, Basal-like) and prognosis. Results: Compared with the control group, Metformin group and non-Metformin group presented more patients with an older age and post-menopause state (P<0.05), but the latter two groups were not significantly different (P > 0.05). Patients in Metformin group and non-Metformin group had higher clinical staging and histological differentiation and more cases of Basal-like subtype than those in the control group (P < 0.05), without significant difference between those two groups (P > 0.05). More cases of local relapse, lymphatic and distant metastasis were seen in Metformin and non-Metformin groups, but the differences were not significant (P > 0.05). Both groups had lower 5-year survival rates than the control group (P < 0.05). Metformin group had a higher overall survival rate as well as a survival rate free of other lethal reasons than the non-Metformin group (P < 0.05) but was not significantly different from the control group in the survival rate free of other lethal reasons (P > 0.05). Conclusions: Type-2 diabetes remains one of the risk factors affecting breast cancer development, progress and prognosis, which could lower the 5-year overall survival rate among breast cancer patients. This is especially evident among menopaused women. Metformin therapy may improve the prognosis of patients of breast cancer complicated with Type-2 diabetes.

Effect of glucagon-like peptide-1 receptor agonists on fat distribution in patients with type 2 diabetes: A systematic review and meta-analysis.

AIMS/INTRODUCTION: Glucagon-like peptide-1 receptor agonists (GLP-1Ras) are widely used to treat type 2 diabetes. They not only reduce glucose, but also have a positive effect on weight loss. However, few studies have reported the effect of GLP-1Ras on fat distribution. MATERIALS AND METHODS: PubMed, Cochrane, Embase and ClinicalTrials.gov were searched for randomized controlled trials on GLP-1Ras and type 2 diabetes, published from inception to June 2021. Our main outcomes were the reductions of visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). Other anthropometric outcomes were also assessed. We used the Cochrane Collaboration tools to assess the risk of bias in the included studies. The quality of the evidence was assessed using the Grades of Recommendation, Assessment, Development and Evaluation profiler version 3.6. Review Manager 5.4.1 and Stata 16.0 were used for data analysis. RESULTS: A total of 10 studies involving 541 patients were included. Compared with the control groups, the GLP-1Ras groups showed reductions in VAT (standard mean difference -0.54, 95% confidence interval [CI] -0.92, -0.17, I2 = 79%, P = 0.005) and SAT (standard mean difference -0.44, 95% CI -0.60, -0.27, I2 = 44%, P < 0.00001). In addition, bodyweight (weighted mean difference -3.59, 95% CI -4.30, -2.88, I2 = 0%, P < 0.00001), waist circumference (weighted mean difference -3.09, 95% CI -4.66, -1.52, I2 = 70%, P = 0.0001) and body mass index (weighted mean difference -1.11, 95% CI -1.35, -0.86, I2 = 47%, P < 0.00001) were significantly decreased. According to the Grades of Recommendation, Assessment, Development and Evaluation approach, the level of evidence was low or moderate. CONCLUSION: This study highlights that GLP-1Ras, especially liraglutide and exenatide, might play an active role in fat distribution in patients with type 2 diabetes. After treatment with GLP-1Ras, both VAT and SAT decreased, and the decrease of VAT was numerically greater than that of SAT.

Expressions of IGF-1R and Ki-67 in breast cancer patients with diabetes mellitus and an analysis of biological characteristics.

OBJECTIVES: There is a cross-link of insulin and insulin-like growth factor-1 (IGF-1) with each other's receptors. The present study was carried out to explore the relationship of Type-2 diabetes mellitus (T2DM) with the occurrence and development of breast cancer by analyzing the expression of IGF-1R and Ki-67, as well as the biological characteristics in breast cancer patients with and without diabetes mellitus. METHODS: A total of 102 cases of breast cancer patients with T2DM admitted in Hebei General Hospital from January 2019 to December 2020 were selected and grouped in T2DM group. While the control group included 106 cases of breast cancer patients without diabetes mellitus in the same period. Further comparison was conducted focusing on the general data, clinical stage, tumor histological grade, molecular classification and prognosis, and the expressions of IGF-1R and Ki-67 in breast cancer tissue between groups. RESULTS: Compared with control group, patients in T2DM group were elderly and accounted for a larger proportion of post-menopause (p<0.05), yet with no significant difference in body mass and family history (p>0.05). Compared with control group, T2DM group had advanced clinical stage, higher histological grade, and more common molecular type, with statistical differences between groups (p<0.05). Furthermore, there were higher proportions of local recurrence, lymph node metastasis and distant metastasis in T2DM group than those in control group, yet with no statistical significance (p>0.05). While statistical difference was found in the comparison of the 5-year survival rate, which was lower in T2DM group than that in control group (p<0.05). In addition, compared with control group, there were significant increase in both the expressions of IGF-1R and Ki-67 in T2DM group (p<0.05). CONCLUSIONS: T2DM may be one of the risk factors affecting the occurrence, development and prognosis of breast cancer, which may decrease the 5-year survival of breast cancer patients. Besides, high expressions of IGF-1R and Ki-67 may be the key factors for poor prognosis of breast cancer patients with diabetes mellitus.

Small-Nanostructure-Size-Limited Phonon Transport within Composite Films Made of Single-Wall Carbon Nanotubes and Reduced Graphene Oxides.

Nanocarbon materials have been widely used for nanoelectronics and energy-related applications. In this work, composite films consisting of reduced graphene oxides (rGOs) and single-wall carbon nanotubes (SWCNTs) are synthesized and studied for their in-plane thermal conductivities. Different from pristine carbon nanotubes or graphene with decreased thermal conductivities above 300 K, the in-plane thermal conductivities of these composite films are found to follow the trend of the specific heat of graphene from 100 to 400 K, i.e., monotonously increasing at elevated temperatures. Such a trend can often be found within amorphous solids but has seldom been observed for nanocarbon. This unique temperature dependence of thermal conductivities is attributed to the largely restricted phonon mean free paths within the graphene sheets that mainly contribute to the in-plane thermal transport. The highest in-plane thermal conductivity among samples with different synthesis conditions is 62.8 W/(m·K) at 300 K. Such a high thermal conductivity, combined with its unique temperature dependency, can be ideal for applications such as flexible film-like thermal diodes based on the junction between two materials with a large contrast for their temperature dependence of the thermal conductivity.

[Effects of light intensity on cleaning away heat property of Viola yedoensis].

The current study was conducted to explore the effects of light intensity in cultivating environment on the cleaning away heat property of Viola yedoensis. In the present study, we established the acute inflammation model of ICR mice by injecting carrageenan. We compared the effects of V. yedoensis grown under different light intensities(100%, 80%, 50%, 35% and 5% of full sunlight) on mice body temperature, thermal radiation and the swelling degree of foot tissue before and after modeling observing by thermal infrared imaging technique and weighing method. The changes of energy metabolism related enzymes in liver were detected by enzyme-linked immunosorbent assay(ELISA). In addition, the effects of V. yedoensis grown under different light intensities on human lung cancer cell A549 proliferation were explored with MTT method. The results showed that the body temperature of all groups of mice in V. yedoensis group were significantly lower than that of the blank group, except 5% full sunlight group, and the body temperature declined in positive proportion to light intensity. V. yedoensis group could alleviate foot swelling, reduce SDH activity in liver tissue(especially 100% full sunlight group and 80% full sunlight group were significantly lower than model group), and the degree of alleviating and reducing was positively correlated with light intensity. There was no significant difference in the activity of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase in liver tissue among treatments. The contents of IL-1ß, IL-6, TNF-α, PGE_2 in foot tissue of mice in V. yedoensis groups were significantly lower than those in model group. Among them, the lowest levels of IL-1ß, IL-6, TNF-α, PGE_2 were found in 80% full sunlight group, and there was no significant difference in TNF-α among different groups. The effects of V. yedoensis aqueous extract on A549 cell line proliferation inhibition rate increased with the light intensities of V. yedoensis cultivating environment. And the effects of V. yedoensis grown under 100% of full sunlight showed significantly higher A549 cell line proliferation inhibition rate compared with other groups(P<0.05). In summary, the light intensity of V. yedoensis cultivating environment is positively correlated with the cleaning away heat property of V. yedoensis, which conforms to the "light-cold and heat property" hypothesis,The V. yedoensis should be planted under full light according.

Marine resource congestion in China: Identifying, measuring, and assessing its impact on sustainable development of the marine economy.

Research on the sustainable development of the marine economy has conventionally revolved around the relationship between efficiency and development. However, most studies have neglected examining how excessive marine resource inputs under certain conditions may lead to resource congestion that restricts output efficiency and sustainable development. To fill this research gap, we optimized an index system to evaluate the input level of marine resources. Using the data of 11 coastal provinces and cities in China from 2000 to 2016, we calculated the congestion of marine resources and analyzed its spatiotemporal evolution and primary influencing factors. Finally, we separated the inefficiency driven by congestion from pure technical inefficiency. The results showed the following: (1) Grave, long-term marine resource congestion does exist in China, and it has evolved from fast to slow, strong to weak, and agglomeration to dispersion; (2) Congestion in the coastal areas has gradually weakened from north to south, and the center of gravity has experienced a shift from the center of China toward the north; (3) Marine resource congestion is mainly affected by the input of resource and capital, resource endowment, and industrial structure; (4) Factors leading to inefficiencies include resource congestion and long-term pure technical inefficiency. By combining congestion and efficiency, we produce values for studying inefficiency and the sustainable development of the marine economy, with the benefit of providing targeted strategies.

Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots.

We describe a sensitive turn-on fluorescent assay for antioxidants by using fluorescence-tunable graphene quantum dots (GQDs). GQDs exhibited strong fluorescence without dopamine (DA). DA could self-polymerize to a thin polydopamine (PDA) film on the surface of GQDs under alkaline environment, resulting in the fluorescence quenching of GQDs via fluorescence resonance energy transfer (FRET). However, the self-polymerization of DA could be effectively inhibited in the presence of antioxidants including glutathione (GSH), ascorbic acid (AA), cysteine (Cys), and homocysteine (Hcys). Thus, the fluorescence of GQDs restored. The "turn-on" sensing of antioxidants could be achieved with high sensitivity. The detection limit for GSH, AA, Cys, and Hcys could be achieved as low as 2.4 nM, 1.5 nM, 4.2 nM, and 4.4 nM, respectively. Finally, the GQDs@PDA system was applied for monitoring cerebral antioxidants in rat brain microdialysates. This work promises new opportunities to evaluate antioxidant capacity in physiological and pathological fields.

[Effects of MT on growth and gonad development of natural overwintering Whitmania pigra].

The objectives of study were to explore the effects of exogenous methyltestosterone( MT) on the growth and gonadal development of overwintering Whitmania pigra. Before overwintering,0. 1,1. 0,10. 0,100. 0,150. 0 µg·L-1 of MT were added to the aquaculture water for 6 weeks. The changes of growth performance,gonad index,endogenous steroid hormones level and internal quality were measured after hibernate for 60 days. Then the tissue slice technique was used to observe the spermary( ovary) of Wh. pigra.The results showed that the body weight,survival rate and gonadal index increased first and then decreased with the increase of exogenous MT concentration; the male gland index was found the highest at the concentration of MT 10. 0 µg·L-1 and the female gland index was the highest at the concentration of MT 1. 0 µg·L-1. The survival rate of Wh. pigra peaked at the concentration of MT 10. 0 µg·L-1.The weight reaches a peak at a concentration of MT 100. 0 µg·L-1( P<0. 05). The number of primary spermatocytes in the testis was negatively correlated with the concentration of exogenous MT. The number of secondary spermatocytes and sperm cells increased first and then decreased. The concentration of secondary spermatocytes was the highest when the concentration of MT was 100. 0 µg·L-1.The number and volume of oocytes in the ovary and the yolk granules increased first and then decreased with the increase of exogenous MT concentration,and the highest was observed at the MT concentration of 100. 0 µg·L-1. The endogenous steroid hormone of Wh.pigra increased first and then decreased with the increase of exogenous MT concentration. The concentration of androgen and progesterone was the highest in MT 100. 0 µg·L-1 treatment( P<0. 05),and the concentration of estrogen was found the highest in MT 10. 0 µg·L-1 treatment( P<0. 05). After adding exogenous MT,Wh. pigra moisture content,acid-insoluble ash content,p H and anti-thrombin activity met the quality criterion of medicinal Wh. pigra in Chinese Pharmacopoeia( 2015 edition). In conclusion,the short-term addition of 1. 0-100. 0 µg·L-1 exogenous MT before hibernation can promote the growth,the development of sperm cells and the antithrombin activity of Wh. pigra.

[Effects of 17ß-E2 on growth and gonad development of overwintering Whitmania pigra].

The present study was conducted to explores the effects of short-term addition of 17ß-E2 on the growth,gonad development and internal quality of overwintering Whitmania pigra. Before overwintering,0. 0,1. 0,10. 0,25. 0,50. 0,100. 0 µg·L~(-1) of 17ß-E2 were added to the aquaculture water for 6 weeks and then hibernated for 60 days. The changes of growth performance,gonad index,morphological structure of spermary( ovary),endogenous steroid hormones level and internal quality were measured. The results showed that the body weight,weight gain rate,specific growth rate,female gonad index,oocyte development and endogenous estrogen level of the leech increased first and then decreased with the increase of the concentration of exogenous 17ß-E2,which were higher than those of the control group. The body weight,weight gain rate and specific growth rate of the leech at the concentration of 25 µg·L~(-1)17ß-E2 were significantly higher than those of the other groups( P<0. 05),oocyte development and endogenous estrogen levels were significantly higher than those of other groups at the concentration of 50 µg·L~(-1)( P<0. 05). When the concentration of exogenous 17ß-E2 was higher than 50 µg·L~(-1),the levels of male gonad index,spermatocyte development,endogenous androgen and progesterone were significantly inhibited( P< 0. 05). There was no significant difference in endogenous corticosteroid levels among the groups. In conclusion,short-term addition of exogenous 17ß-E2 of 10-25 µg·L~(-1) could promote the growth of overwintering leeches,oocyte development and antithrombin activity without inhibiting the development of male gonads.