RESUMEN
BACKGROUND: Oral squamous cell carcinoma is a malignant tumor which is particularly common in the developing world, mostly in older males. OBJECTIVE: Although gene expression analyses had been performed previously, to our best knowledge, systemic co-expression analysis for this disease is still lacking to date. METHODS: In this study, we built the co-expression modules with the help of Weighted Correlation Network Analysis (WGCNA) and investigated the function enrichment of co-expression genes from important modules by bioinformatics analysis. RESULTS: A total of 10 co-expression modules were conducted for 4500 genes from 167 oral squamous cell carcinoma samples. Number of genes for each module ranged from 52 to 1112, with the mean of 450. Interaction relationships of hub-genes between pairwise modules showed great differences, suggesting the high confidence of modules. Functional enrichments of the co-expression modules exhibited great differences. Furthermore, genes in the module ME blue and module ME magenta significantly enriched in hsa05332 (Graft-versus-host disease) and hsa05330 (Allograft rejection), and the two pathways were associated with the oral squamous cell carcinoma. CONCLUSION: Together, our findings provided the framework of co-expression gene modules of oral squamous cell carcinoma and further understanding of these modules at functional aspect.
Asunto(s)
Carcinoma de Células Escamosas/genética , Neoplasias de la Boca/genética , Carcinoma de Células Escamosas/patología , Biología Computacional , Bases de Datos Genéticas , Perfilación de la Expresión Génica , Regulación Neoplásica de la Expresión Génica/genética , Redes Reguladoras de Genes/genética , Rechazo de Injerto/genética , Rechazo de Injerto/patología , Enfermedad Injerto contra Huésped/genética , Enfermedad Injerto contra Huésped/patología , Humanos , Neoplasias de la Boca/patología , Proteínas de Neoplasias/genéticaAsunto(s)
Virus de la Hepatitis B/efectos de los fármacos , Hepatitis B Crónica/tratamiento farmacológico , Inhibidores de la Transcriptasa Inversa/administración & dosificación , Adenina/administración & dosificación , Adenina/análogos & derivados , Adulto , ADN Viral/sangre , Esquema de Medicación , Farmacorresistencia Viral , Quimioterapia Combinada , Femenino , Antígenos e de la Hepatitis B/sangre , Hepatitis B Crónica/sangre , Humanos , Lamivudine/administración & dosificación , Masculino , Cumplimiento de la Medicación , Organofosfonatos/administración & dosificación , Respuesta Virológica Sostenida , Telbivudina , Timidina/administración & dosificación , Timidina/análogos & derivadosRESUMEN
Two cryoprotectant solutions, including trimethylamine oxide (TMAO) and dimethyl sulfoxide (DMSO), and several loading durations were used to evaluate the cryopreservation of the shoot tip of Eriobotrya plants. The best results for regrowth (59.91%) were obtained from 10% TMAO compared to 10% DMSO as cryoprotectant, although nonsignificant differences were found for survival between the two cryoprotectants. We detected pronounced effects of loading duration on survival and regrowth rates of shoot tips. The maximum regrowth (56.36%) was observed at 9 h of loading duration. The cryoprotectants and loading durations greatly affected the regrowth of Eriobotrya shoot tips, and TMAO could be introduced as a nontoxic and efficient cryoprotectant. These results could lay a foundation for the cryopreservation of Eriobotrya.