Control of nitrogen and odor emissions during chicken manure composting with a carbon-based microbial inoculant and a biotrickling filter.

Although aerobic composting is usually utilized in livestock manure disposal, the emission of odorous gases from compost not only induces harm to the human body and the environment, but also causes loss of nitrogen, sulfur, and other essential elements, resulting in a decline in product quality. The impact of biotrickling filter (BTF) and insertion of carbon-based microbial agent (CBMA) on compost maturation, odor emissions, and microbial population during the chicken manure composting were assessed in the current experiment. Compared with the CK group, CBMA addition accelerated the increase in pile temperature (EG group reached maximum temperature 10 days earlier than CK group), increased compost maturation (GI showed the highest increase of 41.3% on day 14 in EG group), resulted in 36.59% and 14.60% increase in NO3--N content and the total nitrogen retention preservation rate after composting. The deodorization effect of biotrickling filter was stable, and the removal rates of NH3, H2S, and TVOCs reached more than 90%, 96%, and 56%, respectively. Furthermore, microbial sequencing showed that CBMA effectively changed the microbial community in compost, protected the ammonia-oxidizing microorganisms, and strengthened the nitrification of the compost. In addition, the nitrifying and denitrifying bacteria were more active in the cooling period than they were in the thermophilic period. Moreover, the abundance of denitrification genes containing nirS, nirK, and nosZ in EG group was lower than that in CK group. Thus, a large amount of nitrogen was retained under the combined drive of BTF and CBMA during composting. This study made significant contributions to our understanding of how to compost livestock manure while reducing releases of odors and raising compost quality.

Fe-doped g-C3N4 with duel active sites for ultrafast degradation of organic pollutants via visible-light-driven photo-Fenton reaction: Insight into the performance, kinetics, and mechanism.

The photo-Fenton process provides a sustainable and cost-effective strategy for removing refractory organic contaminants in wastewater. Herein, a high-efficient Fe-doped g-C3N4 photocatalyst (Fe@CN10) with a unique 3D porous mesh structure was prepared by one-pot thermal polymerization for ultrafast degradation of azo dyes, antibiotics, and phenolic acids in heterogeneous photo-Fenton systems under visible light irradiation. Fe@CN10 exhibited a synergy between adsorption-degradation processes due to the co-existence of Fe3C and Fe3N active sites. Specifically, Fe3C acted as an adsorption site for pollutant and H2O2 molecules, while Fe3N acted as a photocatalytic active site for the high-efficient degradation of MO. Resultingly, Fe@CN10 showed a photocatalytic degradation rate of MO up to 140.32 mg/L min-1. The dominant ROS contributed to the removal of MO in the photo-Fenton pathway was hydroxyl radical (•OH). Surprisingly, as the key reactive species, singlet oxygen (1O2) generated from superoxide radical (•O2-) also efficiently attacked MO in a photo-self-Fenton pathway. Additionally, sponge/Fe@CN10 was prepared and filled in the continuous flow reactors for nearly 100% degradation of MO over 150 h when treating artificial organic wastewater. This work provided a facile route to prepare highly-active Fe-doped photocatalysts and develop a green photocatalytic system for wastewater treatment in the future.

Ball-milled magnetic sludge biochar enables fast aerobic granulation in anoxic/oxic process for the treatment of coal chemical wastewater.

Coal chemical wastewater (CCW) containing toxic and hazardous matters requires to be treated prior to discharge. Promoting the in-situ formation of magnetic aerobic granular sludge (mAGS) in continuous flow reactor process has a great potential for CCW remediation. However, long granulation time and low stability limit the application of AGS technology. In this study, Fe3O4/sludge biochar (Fe3O4/SC) with biochar matrix derived from coal chemical sludge were applied to facilitate the aerobic granulation in two-stage continuous flow reactors, containing separated anoxic and oxic reaction units (abbreviated as A/O process). The performance of A/O process was evaluated at various hydraulic retention times (HRTs) (42 h, 27 h, and 15 h). Magnetic Fe3O4/SC with porous structures, high specific surface area (BET = 96.69 m2/g), and abundant functional groups was successfully prepared by ball-milled method. Adding magnetic Fe3O4/SC to A/O process could promote aerobic granulation (85 days) and the removal of chemical oxygen demand (COD), ammonia nitrogen (NH4+-N), and total nitrogen (TN) from CCW at all tested HRTs. Since the formed mAGS had high biomass, good settling ability, and high electrochemical activities, mAGS-based A/O process had high tolerance to the decrease of HRT from 42 h to 15 h for CCW treatment. The optimized HRT for A/O process was 27 h, at which Fe3O4/SC addition can result in the increase of COD, NH4+-N and TN removal efficiencies by 2.5 %, 4.7 % and 10.5 %, respectively. Based on 16S rRNA genes sequencing, the relative abundances of genus Nitrosomonas, Hyphomicrobium/Hydrogenophaga and Gaiella in mAGS accounting for nitrification, denitrification as well as COD removal were increased during aerobic granulation. Overall, this study proved that adding Fe3O4/SC to A/O process was effective for facilitating aerobic granulation and CCW treatment.

Enhancing extracellular electron transfer through selective enrichment of Geobacter with Fe@CN-modified carbon-based anode in microbial fuel cells.

Microbial fuel cells (MFCs) have been demonstrated as a renewable energy strategy to efficiently recover chemical energy stored in wastewater into clean electricity, yet the limited power density limits their practical application. Here, Fe-doped carbon and nitrogen (Fe@CN) nanoparticles were synthesized by a direct pyrolysis process, which was further decorated to fabricate Fe@CN carbon paper anode. The modified Fe@CN anode with a higher electrochemically active surface area was not only benefit for the adhesion of electrochemically active microorganisms (EAMs) and extracellular electron transfer (EET) between the anode and EAMs but also selectively enriched Geobacter, a typical EAMs species. Accordingly, the MFCs with Fe@CN anode successfully achieved a highest voltage output of 792.76 mV and a prolonged stable voltage output of 300 h based on the mixed culture feeding with acetate. Most importantly, the electroactive biofilms on Fe@CN anode achieved more content ratio of proteins to polysaccharides (1.40) in extracellular polymeric substances for the balance between EET and cell protection under a harsh environment. This work demonstrated the feasibility of development on anode catalysts for the elaboration of the catalytic principle about interface modification, which may contribute to the practical application of MFC in energy generation and wastewater treatment.

An ozone catalytic oxidation system for the degradation of organic compounds in secondary wastewater from refining and chemical processes.

To overcome the low catalytic efficiency, insufficient catalyst strength, and poor ozone circulation in the advanced treatment of secondary wastewater, a hollow cylindrical Fe-Cu-Ce-Mn/Al2O3 catalyst was prepared by the step impregnation method. Compared with the common impregnation method, the step impregnation method produced a more uniform and compact distribution of each metal element, which was more conducive to generating the synergistic effect of various metals; thus, increasing the mineralization rate of organic matter. The hollow cylindrical design ensured the strength of the catalyst and the circulation of ozone. The reduction of the chemical oxygen demand (COD) was compared under different experimental conditions, with the optimal conditions found to be an ozone contact time of 40 min and ozone dosage of 40 mg/L. The average COD, ammonia nitrogen, and ultraviolet absorbance at 254 nm (UV254) removal rates were 36%, 19%, and 20%, respectively. After 3 weeks of continuous experiment, the removal rate was still high. Following analysis by three dimensional fluorescence, GC-MS, and the molecular weight detection of water samples before and after treatment, it was found that the catalyst enhanced the effect of ozone on wastewater treatment, with a significant removal of tryptophan-like aromatic proteins and soluble microbial metabolites, and the removal of most of the small molecular organic matter. In addition, part of the refractory organic matter could be converted into easily degradable organic matter, which greatly improved the biodegradability and mineralization rate of wastewater, and provided good conditions for subsequent treatment.

Effective degradation of chloramphenicol in wastewater by activated peroxymonosulfate with Fe-rich porous biochar derived from petrochemical sludge.

Excess sludge produced from biological wastewater treatment plant in petroleum industry is a kind of hazardous solid waste. Converting the sludge into biochar catalysts may help to reduce its environmental risk, recover resources and increase economic efficiency. However, the role of the sludge biochar in persulfate activation remains unclear, limiting its application in removing organic pollutants from water body. In this study, metal-rich petrochemical sludge was used to produce activated sludge biochar (ASC) via a two-step method of pyrolytic carbonization (400 °C-800 °C) and subsequent KOH activation (abbreviated as ASC 400-800). The physio-chemical properties of ASC 400-800 were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) and Raman. The chloramphenicol (CAP) removal performances of ASC 400-800/peroxymonosulfate (PMS) systems were evaluated. Results showed that porous sludge biochar was successfully prepared by the two-step method. At 800 °C, the specific surface area of ASC reached the highest value of 202.92 m2 g-1. At 600-800 °C, Fe3O4, Fe0, and graphitized carbon were formed in ASC. Among ASC 400-800, ASC 800 exhibited the best CAP removal performance in ASC 800/PMS system by adsorption combined with catalytic degradation. The optimal conditions identified for 0.31 mM CAP removal were ASC 800 2.0 g L-1, PMS 6.2 mM, and pH 2.0. SO4•-, •OH, and 1O2 may contribute to CAP degradation. The degradation pathways of CAP were proposed based on the identified degradation intermediates. Overall, this study confirmed that porous biochar derived from petrochemical sludge was an effective adsorbent or PMS catalyst to remove organic pollutants from wastewater.

Effects of hydrochar and biogas slurry reflux on methane production by mixed anaerobic digestion of cow manure and corn straw.

This study aimed to enhance methane production from mixed anaerobic digestion of cow manure and corn straw by adding hydrochar and biogas slurry reflux. The hydrochar characterization revealed that it can provide attachment for microbial growth, and abundant surface functional groups (such as C-O, CO, C-OH, and C-N) for adsorption. Direct interspecies electron transfer (DIET) mediated by surface oxygen-containing functional groups on hydrochar increased the methane yield. The experimental group added with hydrochar and biogas slurry reflux had the highest methane and biogas production (34.40% and 36.98% higher than the control group, respectively). Results demonstrate hydrochar and biogas slurry reflux can improve microorganism species richness in anaerobic digestion systems, in which hydrochar can also improve microorganism species uniformity. Distance-based redundancy analysis showed that the VFAs, and pH had the greatest effects on the composition of the microbial community. The dominant microorganism at the phylum level in AD system were Bacteroidetes, Firmicutes, and Proteobacteria. The addition of hydrochar and biogas slurry reflux can significantly increase the species abundance of Methanobacterium. These results indicate that the addition of hydrochar and biogas slurry reflux can improve the corresponding microbial abundance, in which hydrochar can enhance the redox characteristics and DIET between microorganism, biogas slurry reflux can also increase nutrient content of anaerobic digestion system, and collectively promote the methane yield.

Quorum sensing signals improve the power performance and chlortetracycline degradation efficiency of mixed-culture electroactive biofilms.

Electroactive biofilms (EABs) play an important role in bioelectrochemical systems due to their abilities to generate electrons and perform extracellular electron transfer (EET). Here, we investigated the effects of quorum sensing (QS) signals on power output, chlortetracycline degradation, and structure of EABs in MFCs treating antibiotic wastewater. The voltage output of MFCs with C4-HSL and PQS increased by 21.57% and 13.73%, respectively, compared with that without QS signals. The chlortetracycline degradation efficiency in closed-circuit MFCs with C4-HSL and PQS increased by 56.53% and 50.04%, respectively, which resulted from the thicker biofilms, higher biomass, and stronger activities. Additionally, QS signals induced the heterogeneous distribution of EPS for a balance between self-protection and EET under environmental pressure. Geobacter prevailed by the addition of QS signals to resist high chlortetracycline concentration. Our results provided a broader understanding on regulating EABs within electrode interface to improve their performance for environmental remediation and clean energy development.

Effect of Polyaniline and Graphene Oxide Modified Carbon Felt on Adsorption and Immobilization of Acidithiobacillus ferrooxidans.

Biological desulfurization plays an increasingly important role in desulfurization industry. A strain of Acidithiobacillus ferrooxidans ZJ-2 with high Fe2+ oxidizing efficiency was in this study isolated and screened to remove hydrogen sulfide from biogas. To further improve its oxidation efficiency, A. ferrooxidans ZJ-2 was immobilized using carbon felt (CF), modified with graphene oxide (GO) and polyaniline (PANI), as immobilized carrier. The effects of immobilization on strain's Fe2+ oxidation efficiency and impact of PANI and GO on CF were also investigated. Raman spectra and atomic force microscopy showed that CF was successfully modified using GO and PANI. Cyclic voltammetry and electrochemical impedance spectroscopy measurements revealed that the electrochemical properties of modified CF were improved, presenting the following trend in conductivity: CF< GO-modified CF (GO-CF) < PANI-modified CF (PANI-CF) < PANI/GO-modified CF (PANI/GO-CF). The resistance of modified CF was lower than that of unmodified CF, and exhibited the following trend: CF > GO-CF > PANI-CF > GO/PANI-CF. While PANI-CF inhibited growth of free and immobilized A. ferrooxidans ZJ-2, GO-CF was conducive to microbial growth and increased cell density and oxidation ability of A. ferrooxidans ZJ-2. Thus, the present study developed an immobilized bacterial carrier that had better conductivity and lower resistance and was efficient in immobilizing A. ferrooxidans and could be used for biogas desulfurization in biological and biochemical combined reactors.

Effect of biochar combined with a biotrickling filter on deodorization, nitrogen retention, and microbial community succession during chicken manure composting.

The high-nitrogen content and dense structure of poultry manure compost cause volatilization of N to ammonia (NH3). This study evaluated the combined application of biochar and biotrickling filtration (BTF) to remove of odor in chicken manure mixed straw compost (w/w, 2.5:1). Adding of 10% biochar reduced NH3, hydrogen sulfide (H2S), and total volatile organic compounds (TVOCs) contents by 20.04%, 16.18%, and 17.55% respectively, and decreased the N loss rate by 8.27%, compared with those observed in control. The organic matter content decreased by 28.11% and germination index reached 97.36% in the experimental group. Meanwhile, the N-cycling microorganisms such as Pusillimonas and Pseudomonas became more active, and the relative abundance of sulfur-cycling microorganisms Hydrogenispora decreased in the experimental group. Following BTF application, the NH3, H2S, and TVOCs removal rates reached 95%, 97%, and 53%, respectively.

Effects of granular activated carbon and temperature on the viscosity and methane yield of anaerobically digested of corn straw with different dry matter concentrations.

Anaerobic digestion (AD) systems with high substrate concentrations are characterized by high viscosity, which affects material and energy transfer efficiencies, thereby influencing methane production efficiency. In this study, adding granular activated carbon (GAC) and increasing the temperature decreased the viscosity by 4.56-10.19% and 27.13-28.85%, respectively, and improved AD efficiency. Adding GAC and increasing the temperature enhanced the methane yields by 34.37-38.15% and 25.60-28.31%, respectively. Distance-based redundancy analysis showed that the viscosity, temperature, and GAC had the greatest effects on the composition of the microbial community. The dominant bacteria in the medium-temperature AD system at the phylum level belonged to Firmicutes, Bacteroidetes, and Euryarchaeota. In addition to the dominant bacteria in the medium-temperature AD system, the thermophilic phylum Thermotogae was abundant in the high-temperature AD system. Moreover, the relative abundance of Euryarchaeota, which contained most of the methanogens, was higher in the high-temperature AD system than in the medium-temperature AD system.

Production of poly-γ-glutamic acid (γ-PGA) from xylose-glucose mixtures by Bacillus amyloliquefaciens C1.

Due to the promising applications, the demand to enhance poly-γ-glutamic acid (γ-PGA) production while decreasing the cost has increased in the past decade. Here, xylose/glucose mixture and corncob hydrolysate (CCH) was evaluated as alternatives for γ-PGA production by Bacillus amyloliquefaciens C1. Although both have been validated to support cell growth, glucose and xylose were not simutaneously consumed and exhibited a diauxic growth pattern due to carbon catabolite repression (CCR) in B. amyloliquefaciens C1, while the enhanced transcription of araE alleviated the xylose transport bottleneck across a cellular membrane. Additionally, the xyl operon (xylA and xylB), which was responsible for xylose metabolism, was strongly induced by xylose at the transcriptional level. When cultured in a mixed medium, xylR was sharply induced to 3.39-folds during the first 8-h while reduced to the base level similar to that in xylose medium. Finally, pre-treated CCH mainly contained a mixture of glucose and xylose was employed for γ-PGA fermentation, which obtained a final concentration of 6.56 ± 0.27 g/L. Although the glucose utilization rate (84.91 ± 1.81%) was lower than that with chemical substrates, the xylose utilization rate (43.41 ± 2.14%) and the sodium glutamate conversion rate (77.22%) of CCH were acceptable. Our study provided a promising approach for the green production of γ-PGA from lignocellulosic biomass and circumvent excessive non-food usage of glucose.

Enhancing biomethane production and pyrene biodegradation by addition of bio-nano FeS or magnetic carbon during sludge anaerobic digestion.

Pyrene exerts toxic effects on methanogens during anaerobic digestion of sludge, thus affecting the efficiency of sludge treatment. This study evaluated the facilitated direct interspecific electron transfer (DIET) between bacteria and methanogens when bio-nano FeS or magnetic carbon is added into anaerobic reactors. Results showed that adding 200 mg/L bio-nano FeS or magnetic carbon clearly reduced the accumulation of short-chain fatty acids and avoided acidification during 25 days of anaerobic digestion. The methane productions were 98.38 L/kg total solid (TS) and 73.69 L/kg TS in the bio-nano FeS and magnetic carbon systems, respectively, which accelerated methane production by 58.1% and 33.4%, respectively, compared with the control system (40.26 L/kg TS). The pyrene removal rates reached 77.5% and 72.1% in the bio-nano FeS and magnetic carbon systems, whereas it was only 40.8% in the control system. Analysis of microbial community structure revealed that methanogens (e.g. Methanosarcina and Methanosaeta) and extracellular electron-transfer bacteria (e.g. Pseudomonas, Cloastridia, and Synergistetes) were enriched in the reactors added with bio-nano FeS or magnetic carbon. This result indicates that the addition of bio-nano FeS or magnetic carbon may promote the activity and growth of microorganisms to improve the efficiency of methane production and pyrene degradation by enhancing DIET.

A novel core-shell Fe@Co nanoparticles uniformly modified graphite felt cathode (Fe@Co/GF) for efficient bio-electro-Fenton degradation of phenolic compounds.

In this study, a core-shell Fe@Co nanoparticles uniformly modified graphite felt (Fe@Co/GF) was fabricated as the cathode by one-pot self-assembly strategy for the degradation of vanillic acid (VA), syringic acid (SA), and 4-hydroxybenzoic acid (HBA) in the Bio-Electro-Fenton (BEF) system. The Fe@Co/GF cathode showed dual advantages with excellent electrochemical performance and catalytic reactivity not only due to the high electron transfer efficiency but also the synergistic redox cycles between Fe and Co species, both of which significantly enhanced the in situ generation of H2O2 and hydroxyl radicals (OH) to 152.40 µmol/L and 138.48 µmol/L, respectively. In this case, the degradation rates of VA, SA, and HBA reached 100, 94.32, and 100%, respectively, within 22 h. Representatively, VA was degraded and ultimately mineralized via demethylation, decarboxylation and ring-opening reactions. This work provided a promising approach for eliminating typical recalcitrant organic pollutants generated by the pre-treatment of lignocellulose resources.

Coordinated response of Au-NPs/rGO modified electroactive biofilms under phenolic compounds shock: Comprehensive analysis from architecture, composition, and activity.

Electroactive biofilms (EABs) can be integrated with conductive nanomaterials to boost extracellular electron transfer (EET) for achieving efficient waste treatment and energy conversion in bioelectrochemical systems. However, the in situ nanomaterial-modified EABs of mixed-culture, and their response under environmental stress are rarely revealed. Here, two nanocatalyst-decorated EABs were established by self-assembled Au nanoparticles-reduced graphene oxide (Au-NPs/rGO) in mixed-biofilms with different maturities, then their multi-property were analyzed under long-term phenolic shock. Results showed that the power density of Au-NPs/rGO decorated EABs was significantly enhanced by 28.66-42.82% due to the intensified EET pathways inside biofilms. Meanwhile, the electrochemical and catalytic performance of EABs were controllably regulated by 0.3-3.0 g/L phenolic compounds, which, however, resulted in differential alterations in their architecture, composition, and viability. EABs originated with higher maturity displayed more compact structure, lower thickness (110 µm), higher biomass (8.67 mg/cm2) and viability (0.85-0.91), endowing it better antishock ability to phenolic compounds. Phenolic-shock also induced the heterogeneous distribution of extracellular polymeric substances in terms of both spatial and bonding degrees of the decorated EABs, which could be regarded as an active response to strike a balance between self-protection and EET under environmental pressure. Our findings provide a broader understanding of microbe-electrode interactions in the micro-ecology interface and improve their performance in the removal of complex contaminants for sustainable remediation and new-energy development.

Biosynthesized iron sulfide nanoparticles by mixed consortia for enhanced extracellular electron transfer in a microbial fuel cell.

The bioanode of mixed consortia was for the first time used to in-situ synthesize iron sulfide nanoparticles in a microbial fuel cell (MFC) over a long-term period (46 days). These poorly crystalline nanoparticles with an average size of 29.97 ± 7.1 nm, comprising of FeS and FeS2, significantly promoted extracellular electron transfer and thus the electricity generation of the MFC. A maximum power density of 519.00 mW/m2 was obtained from the MFC, which was 1.92 times as high as that of the control. The cell viability was promoted by a small amount of iron sulfide nanoparticles but inhibited by the thick nanoparticle "shell" covered on the bacterial cells. Some electroactive and sulfur reducing bacteria (eg. Enterobacteriaceae, Desulfovibrio, and Geobacter) were specifically enriched on the anode. This study provides a novel insight for improving the performance of bioelectrochemical systems through in-situ sustainable nanomaterials biofabrication by mixed consortia.

Effects of copper salts on performance, antibiotic resistance genes, and microbial community during thermophilic anaerobic digestion of swine manure.

This study investigated methane production and ARGs reduction during thermophilic AD of swine manure with the addition of different Cu salts (cupric sulfate, cupric glycinate, and the 1:1 mixture of these two salts). Results showed methane production was increased by 28.78% through adding mixed Cu salts. The mixed Cu group effectively reduced total ARGs abundance by 26.94%, suggesting mixed Cu salts did not promote the potential ARGs risk. The positive effects of mixed Cu salts on AD performance and ARGs removal might be ascribed to the low bioavailability. Microbial community analysis indicated the highest abundances of Clostridia_MBA03 and Methanobacterium in the mixed Cu group might cause the increased methane production. Spearman's rank correlation analysis elucidated the succession in microbial community induced by environmental factors was the main driver for shaping ARGs profiles. Thus, mixed Cu salts could be an alternative to replace the inorganic Cu salt in animal feed additives.

Positive effects of concomitant heavy metals and their reduzates on hexavalent chromium removal in microbial fuel cells.

Cr(vi) laden wastewaters generally comprise a range of multiple heavy metals such as Au(iii) and Cu(ii) with great toxicity. In the present study, cooperative cathode modification by biogenic Au nanoparticles (BioAu) reduced from aqueous Au(iii) and in situ Cu(ii) co-reduction were investigated for the first time to enhance Cr(vi) removal in microbial fuel cells (MFCs). With the co-existence of Cu(ii) in the catholyte, the MFC with carbon cloth modified with nanocomposites of multi-walled carbon nanotubes blended with BioAu (BioAu/MWCNT) obtained the highest Cr(vi) removal rate (4.07 ± 0.01 mg L-1 h-1) and power density (309.34 ± 17.65 mW m-2), which were 2.73 and 3.30 times as high as those for the control, respectively. The enhancements were caused by BioAu/MWCNT composites and deposited reduzates of Cu(ii) on the cathode surface, which increased the adsorption capacity, electronic conductivity and electrocatalytic activity of the cathode. This study provides an alternative approach for efficiently remediating co-contamination of multiple heavy metals and simultaneous bioenergy recovery.

Effects of amino-modified biofilm carriers on biogas production in the anaerobic digestion of corn straw.

This paper studied the property of three different biofilm carriers added into the anaerobic digestion systems, a granular activated carbon, a polyacrylonitrile, and a polyacrylonitrile modified with diethylenetriamine (PAN-NH2). The PAN-NH2 system kept the maximum biogas and methane production, which were 42.69% and 37.29% higher than the control system, respectively. The value of pH and chemical oxygen demand, the content of total solid and volatile solid, volatile fatty acids concentration, coenzyme F420 concentration, and microbial community analysis were investigated during the anaerobic digestion process. The PAN-NH2 system had the highest removal efficiency of the pollutants and regulated the pH of the system better than other systems. The result of high-throughput sequencing analysis showed that the addition of biofilm carriers and mediation with amino-groups adjusted system pH and improved biogas and CH4 production by reducing the relative abundance of bacteria in the hydrolysis/acidogenesis stages. Methanosarcina gradually replaced other methanogens during the experimental runs and was the dominant methanogen at the end of the anaerobic digestion process.

Anaerobic biodegradation of pyrene by Klebsiella sp. LZ6 and its proposed metabolic pathway.

Pyrene is one of the polycyclic aromatic hydrocarbons, which are a potential threat to ecosystems due to their mutagenicity, carcinogenicity, and teratogenicity. In this study, several bacteria were isolated from oil contaminated sludge and their capacity to biodegrade pyrene was investigated. Of these bacteria, the monoculture strain LZ6 showed the highest pyrene anaerobic biodegradation rate of 33% after 30 days when the initial concentration was 50 mg/L, and was identified as Klebsiella sp. LZ6 by morphological observation, the GENIII technology of Biolog, and 16S rDNA gene sequence analysis. The influence of various culture parameters on the biodegradation of pyrene were evaluated, and Klebsiella sp. LZ6 all showed the high degradation rate at an inoculum of 10-20% (v/v), pH 6.0-8.4, temperature 30-38°C, and initial pyrene concentration of 50-150 mg/L. The intermediate metabolites of the anaerobic biodegradation were analyzed by GC-MS. Several metabolites were identified, such as pyrene, 4,5-dihydro-, phenanthrene, dibenzo-p-dioxin, and 4-hydroxycinnamate acid. The anaerobic metabolic pathway for the degradation of pyrene was inferred by the products. It seems that pyrene was first reduced to pyrene,4,5-dihydro- by the adding of two hydrogen atoms, and then the carbon-carbon bond cleavage at saturated carbon atoms generated phenanthrene.