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Synapses are critical actors of neuronal transmission as they form the basis of chemical communication between neurons. Accurate computational models of synaptic dynamics may prove important in elucidating emergent properties across hierarchical scales. Yet, in large-scale neuronal network simulations, synapses are often modeled as highly simplified linear exponential functions due to their small computational footprint. However, these models cannot capture the complex non-linear dynamics that biological synapses exhibit and thus, are insufficient in representing synaptic behavior accurately. Existing detailed mechanistic synapse models can replicate these non-linear dynamics by modeling the underlying kinetics of biological synapses, but their high complexity prevents them from being a suitable option in large-scale models due to long simulation times. This motivates the development of more parsimonious models that can capture the complex non-linear dynamics of synapses accurately while maintaining a minimal computational cost. We propose a look-up table approach that stores precomputed values thereby circumventing most computations at runtime and enabling extremely fast simulations for glutamatergic receptors AMPAr and NMDAr. Our results demonstrate that this methodology is capable of replicating the dynamics of biological synapses as accurately as the mechanistic synapse models while offering up to a 56-fold increase in speed. This powerful approach allows for multi-scale neuronal networks to be simulated at large scales, enabling the investigation of how low-level synaptic activity may lead to changes in high-level phenomena, such as memory and learning.
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The topographic organization of afferents to the hippocampal CA3 subfield are well-studied, but their role in influencing the spatiotemporal dynamics of population activity is not understood. Using a large-scale, computational neuronal network model of the entorhinal-dentate-CA3 system, the effects of the perforant path, mossy fibers, and associational system on the propagation and transformation of network spiking patterns were investigated. A correlation map was constructed to characterize the spatial structure and temporal evolution of pairwise correlations which underlie the emergent patterns found in the population activity. The topographic organization of the associational system gave rise to changes in the spatial correlation structure along the longitudinal and transverse axes of the CA3. The resulting gradients may provide a basis for the known functional organization observed in hippocampus.
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Dysfunction in cholinergic modulation has been linked to a variety of cognitive disorders including Alzheimer's disease. The important role of this neurotransmitter has been explored in a variety of experiments, yet many questions remain unanswered about the contribution of cholinergic modulation to healthy hippocampal function. To address this question, we have developed a model of CA1 pyramidal neuron that takes into consideration muscarinic receptor activation in response to changes in extracellular concentration of acetylcholine and its effects on cellular excitability and downstream intracellular calcium dynamics. This model incorporates a variety of molecular agents to accurately simulate several processes heretofore ignored in computational modeling of CA1 pyramidal neurons. These processes include the inhibition of ionic channels by phospholipid depletion along with the release of calcium from intracellular stores (i.e., the endoplasmic reticulum). This paper describes the model and the methods used to calibrate its behavior to match experimental results. The result of this work is a compartmental model with calibrated mechanisms for simulating the intracellular calcium dynamics of CA1 pyramidal cells with a focus on those related to release from calcium stores in the endoplasmic reticulum. From this model we also make various predictions for how the inhibitory and excitatory responses to cholinergic modulation vary with agonist concentration. This model expands the capabilities of CA1 pyramidal cell models through the explicit modeling of molecular interactions involved in healthy cognitive function and disease. Through this expanded model we come closer to simulating these diseases and gaining the knowledge required to develop novel treatments.
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Biological realism of dendritic morphologies is important for simulating electrical stimulation of brain tissue. By adding point process modeling and conditional sampling to existing generation strategies, we provide a novel means of reproducing the nuanced branching behavior that occurs in different layers of granule cell dendritic morphologies. In this study, a heterogeneous Poisson point process was used to simulate branching events. Conditional distributions were then used to select branch angles depending on the orthogonal distance to the somatic plane. The proposed method was compared to an existing generation tool and a control version of the proposed method that used a homogeneous Poisson point process. Morphologies were generated with each method and then compared to a set of digitally reconstructed neurons. The introduction of a conditionally dependent branching rate resulted in the generation of morphologies that more accurately reproduced the emergent properties of dendritic material per layer, Sholl intersections, and proximal passive current flow. Conditional dependence was critically important for the generation of realistic granule cell dendritic morphologies.
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OBJECTIVE: The network architecture connecting neural regions is defined by the organization and anatomical properties of the projecting axons, but its contributions to neural encoding and system function are difficult to study experimentally. METHODS: Using a large-scale, spiking neuronal network model of rat dentate gyrus, the role of the anatomy of the entorhinal-dentate axonal projection was evaluated in the context of spatial encoding by incorporating grid cell activity to provide physiological, spatially-correlated input. The dorso-ventral extents of the entorhinal axon terminal fields were varied to generate different feedforward architectures, and the resulting spatial representations and spatial information scores of the network were evaluated. Position was decoded from the population activity using a point process filter to investigate the contributions of network architecture on spatial encoding. RESULTS: The model predicted the emergence of anatomical gradients within the dentate gyrus for place field size and spatial information along its dorso-ventral axis, which were dependent on the extents of the entorhinal axon terminal fields. The decoding results revealed an optimal performance at an axon terminal field extent of 2 mm that lies within the biological range. CONCLUSION: The axonal anatomy mediates a tradeoff between encoding multiple place field sizes or achieving a high spatial information score, and the combination of both properties is necessary to maximize spatial encoding by a network. SIGNIFICANCE: In total, this paper establishes a mechanistic neuronal network model that, in concert with information-theoretic and statistical methods, can be used to investigate how lower level properties contribute to higher level function.
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Axones/fisiología , Giro Dentado/fisiología , Corteza Entorrinal/fisiología , Algoritmos , Animales , Axones/ultraestructura , Conducta Animal , Mapeo Encefálico , Simulación por Computador , Giro Dentado/ultraestructura , Corteza Entorrinal/ultraestructura , Modelos Neurológicos , Vías Nerviosas/fisiología , Vías Nerviosas/ultraestructura , Ratas , Navegación Espacial/fisiologíaRESUMEN
Connectivity between neural regions, particularly in the hippocampus, is seldom all-to-all or random, yet it is the predominant method by which connectivity is implemented in most models of neuronal networks. We have been developing a computational platform for simulating the trisynaptic circuit of rat hippocampus with which we have constructed a large-scale, biologically-realistic, spiking neuronal network model of the entorhinal-dentate-CA3 system. Using the model, we had demonstrated a non-trivial effect of topographic connectivity on network dynamics and function. In this work, we detail the introduction of the CA1 subregion to the large-scale model. Using anatomical data, we constrained the distribution of axon collaterals, i.e., Schaffer collaterals, projected from CA3 to CA1 and preserved the topographic organization of the projections. Using a simplified multi-compartmental model of CA1 pyramidal cells and a single compartment model of CA1 parvalbumin basket cells, that were connected with disynaptic feedforward inhibition and feedback inhibition, we demonstrate the network activity of the CA1 network given a topographic organization of Schaffer collaterals. From this introduction of CA1 to the large-scale model, we can then observe the successive transformation of spatio-temporal, spiking neural activity as it propagates through the trisynaptic circuit.
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Región CA1 Hipocampal/fisiología , Modelos Neurológicos , Red Nerviosa , Células Piramidales/fisiología , Animales , Axones/fisiología , Neuronas/fisiología , RatasRESUMEN
Current parametric approaches to dendritic morphology generation are limited in their ability to replicate realistic branching. A non-parametric approach applying a point process filter and the expectation-maximization algorithm offers a data-based solution that estimates the dendritic branching rate based on observations of bifurcation events in real neurons. Point processes can then be simulated using this branching rate estimate to indicate when a generated morphology should branch. Morphologies generated using this technique match both basic and emergent property distributions of the real neurons used as input into the algorithm. Further refinement of branching angles will allow for a flexible tool to generate realistic morphologies of a variety of neuronal stereotypes.
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Simulación por Computador , Dendritas , Neuronas/citología , Algoritmos , HumanosRESUMEN
Synapses are key components in signal transmission in the brain, often exhibiting complex non-linear dynamics. Yet, they are often crudely modelled as linear exponential equations in large-scale neuron network simulations. Mechanistic models that use detailed channel receptor kinetics more closely replicate the nonlinear dynamics observed at synapses, but use of such models are generally restricted to small scale simulations due to their computational complexity. Previously, we have developed an ``input-output'' (IO) synapse model using the Volterra functional series to estimate nonlinear synaptic dynamics. Here, we present an improvement on the IO synapse model using the extbf{Laguerre-Volterra network (LVN) framework. We demonstrate that utilization of the LVN framework helps reduce memory requirements and improves the simulation speed in comparison to the previous iteration of the IO synapse model. We present results that demonstrate the accuracy, memory efficiency, and speed of the LVN model that can be extended to simulations with large numbers of synapses. Our efforts enable complex nonlinear synaptic dynamics to be modelled in large-scale network models, allowing us to explore how synaptic activity may influence network behavior and affects memory, learning, and neurodegenerative diseases.
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Dinámicas no Lineales , Sinapsis , Encéfalo , Simulación por Computador , Cinética , Modelos NeurológicosRESUMEN
Spatial information is encoded by the hippocampus, and the factors that contribute to the amount of information that can be encoded and the transformation of spatial information through the trisynaptic circuit remain an important issue. A large-scale neuronal network model of the rat entorhinal-dentate system was developed with multicompartmental representations of the neurons within the dentate gyrus. Spatial information was introduced to the network via grid cell activity, and the spatial information encoding capabilities of the network were assessed using a recursive decoding algorithm to estimate the position of a virtual rat using the dentate activity. To obtain a measure for the information that the network could convey, decoding error was calculated for different decoding population sizes. Decoding error decreased exponentially as a function of population size. Therefore, the time constant and the asymptote of the error curve could be used as metrics to compare the changes in encoding performance. In conjunction with the large-scale model, this paradigm can be used to characterize how neural properties, network composition, and the interactions between different subfields affect spatial information encoding.
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Giro Dentado , Neuronas , Algoritmos , Animales , Corteza Entorrinal , RatasRESUMEN
OBJECTIVE: The ideal form of a neural-interfacing device is highly dependent upon the anatomy of the region with which it is meant to interface. Multiple-electrode arrays provide a system that can be adapted to various neural geometries. Computational models of stimulating systems have proven useful for evaluating electrode placement and stimulation protocols, but have yet to be adequately adapted to the unique features of the hippocampus. METHODS: As an approach to understanding potential memory restorative devices, an admittance method-NEURON model was constructed to predict the direct and synaptic response of a region of the rat dentate gyrus to electrical stimulation of the perforant path. RESULTS: A validation of estimated local field potentials against experimental recordings is performed and results of a bilinear electrode placement and stimulation amplitude parameter search are presented. CONCLUSION: The parametric analysis presented herein suggests that stimulating electrodes placed between the lateral and medial perforant path, near the crest of the dentate gyrus, yield a larger relative population response to given stimuli. SIGNIFICANCE: Beyond deepening understanding of the hippocampal tissue system, establishment of this model provides a method to evaluate candidate stimulating devices and protocols.
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Giro Dentado , Estimulación Eléctrica/métodos , Modelos Neurológicos , Animales , Giro Dentado/fisiología , Giro Dentado/efectos de la radiación , Capacidad Eléctrica , Impedancia Eléctrica , Electrodos , Neuronas/citología , RatasRESUMEN
PURPOSE: Encephalitis is an inflammation affecting brain parenchyma. At the time of presentation, it may be difficult to differentiate between viral encephalitis and altered mental status or seizures during febrile illness. The aim of the present study is to identify the predictive factors and to determine the prognostic factors of viral encephalitis in children presenting as seizure with fever. METHODS: From the retrospective review of the medical records, children with seizures or altered mental status during febrile illness who presented to Samsung Medical Center between January 2008 and May 2013 were included in the study. RESULTS: 81 patients were enrolled in this study (female:male=32:49). The mean age at admission was 4.9±4.3 years (range 0–14 years old). The patients were categorized into two groups according to the clinical diagnosis: (1) Viral encephalitis (VIRAL ENC, n=66), (2) Complex febrile seizures imitating viral encephalitis(C-FS, n=15). The predictive factors of viral encephalitis were focal and/or lateralized abnormalities in electroencephalography (EEG) (P<0.001). CONCLUSION: EEG can be helpful to predict the viral encephalitis, in pediatric patient who shows delayed restoration of consciousness after seizure during febrile illness.
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Niño , Humanos , Encéfalo , Estado de Conciencia , Diagnóstico , Electroencefalografía , Encefalitis , Encefalitis Viral , Fiebre , Inflamación , Registros Médicos , Estudios Retrospectivos , Convulsiones , Convulsiones FebrilesRESUMEN
Place cells are neurons in the hippocampus that are sensitive to location within an environment. Simulations of a large-scale, computational model of the rat dentate gyrus using grid cell input have been performed resulting in granule cells that express multiple place fields. The typical method of detecting place fields using a global threshold on this data is unreliable as the characteristics of the place fields from a single neuron can be highly variable. A grid-based implementation of DENCLUE has been developed to calculate local thresholds to identify each place field. An adaptive binning algorithm used to smooth the rate maps was combined with the DENCLUE implementation to adaptively choose the size of the smoothing kernel and reduce the number of free parameters of the total algorithm. A sensitivity analysis was performed using the threshold parameter to demonstrate the robustness of using local thresholds as opposed to using a single global threshold in detecting the place fields resulting from the large-scale simulation. The analysis supports the use of applying local thresholds for place field detection and will be used to further investigate the role of granule cells in hippocampal function.
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Análisis por Conglomerados , Giro Dentado/fisiología , Procesamiento de Imagen Asistido por Computador , Modelos Neurológicos , Potenciales de Acción/fisiología , Algoritmos , Animales , Simulación por Computador , Corteza Entorrinal/fisiología , Hipocampo/fisiología , Modelos Estadísticos , Neuronas/fisiología , Distribución de Poisson , RatasRESUMEN
GOAL: This paper describes a million-plus granule cell compartmental model of the rat hippocampal dentate gyrus, including excitatory, perforant path input from the entorhinal cortex, and feedforward and feedback inhibitory input from dentate interneurons. METHODS: The model includes experimentally determined morphological and biophysical properties of granule cells, together with glutamatergic AMPA-like EPSP and GABAergic GABAA-like IPSP synaptic excitatory and inhibitory inputs, respectively. Each granule cell was composed of approximately 200 compartments having passive and active conductances distributed throughout the somatic and dendritic regions. Modeling excitatory input from the entorhinal cortex was guided by axonal transport studies documenting the topographical organization of projections from subregions of the medial and lateral entorhinal cortex, plus other important details of the distribution of glutamatergic inputs to the dentate gyrus. Information contained within previously published maps of this major hippocampal afferent were systematically converted to scales that allowed the topographical distribution and relative synaptic densities of perforant path inputs to be quantitatively estimated for inclusion in the current model. RESULTS: Results showed that when medial and lateral entorhinal cortical neurons maintained Poisson random firing, dentate granule cells expressed, throughout the million-cell network, a robust nonrandom pattern of spiking best described as a spatiotemporal "clustering." To identify the network property or properties responsible for generating such firing "clusters," we progressively eliminated from the model key mechanisms, such as feedforward and feedback inhibition, intrinsic membrane properties underlying rhythmic burst firing, and/or topographical organization of entorhinal afferents. CONCLUSION: Findings conclusively identified topographical organization of inputs as the key element responsible for generating a spatiotemporal distribution of clustered firing. These results uncover a functional organization of perforant path afferents to the dentate gyrus not previously recognized: topography-dependent clusters of granule cell activity as "functional units" or "channels" that organize the processing of entorhinal signals. This modeling study also reveals for the first time how a global signal processing feature of a neural network can evolve from one of its underlying structural characteristics.
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Mapeo Encefálico/métodos , Giro Dentado/citología , Giro Dentado/fisiología , Modelos Neurológicos , Neuronas/fisiología , Animales , Análisis por Conglomerados , Simulación por Computador , RatasRESUMEN
This paper reports on findings from a million-cell granule cell model of the rat dentate gyrus that was used to explore the contributions of local interneuronal and associational circuits to network-level activity. The model contains experimentally derived morphological parameters for granule cells, which each contain approximately 200 compartments, and biophysical parameters for granule cells, basket cells, and mossy cells that were based both on electrophysiological data and previously published models. Synaptic input to cells in the model consisted of glutamatergic AMPA-like EPSPs and GABAergic-like IPSPs from excitatory and inhibitory neurons, respectively. The main source of input to the model was from layer II entorhinal cortical neurons. Network connectivity was constrained by the topography of the system, and was derived from axonal transport studies, which provided details about the spatial spread of axonal terminal fields, as well as how subregions of the medial and lateral entorhinal cortices project to subregions of the dentate gyrus. Results of this study show that strong feedback inhibition from the basket cell population can cause high-frequency rhythmicity in granule cells, while the strength of feedforward inhibition serves to scale the total amount of granule cell activity. Results furthermore show that the topography of local interneuronal circuits can have just as strong an impact on the development of spatio-temporal clusters in the granule cell population as the perforant path topography does, both sharpening existing clusters and introducing new ones with a greater spatial extent. Finally, results show that the interactions between the inhibitory and associational loops can cause high frequency oscillations that are modulated by a low-frequency oscillatory signal. These results serve to further illustrate the importance of topographical constraints on a global signal processing feature of a neural network, while also illustrating how rich spatio-temporal and oscillatory dynamics can evolve from a relatively small number of interacting local circuits.
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The correlation due to different topographies was characterized in a large-scale, biologically-realistic, computational model of the rat hippocampus using a spatio-temporal correlation analysis. The effect of the topographical projection between the following subregions of the hippocampus was investigated: the entorhinal to dentate projection, the entorhinal to CA3 projection, and the mossy fiber to CA3 projection. Through this work, analysis was performed on the individual and combined effects of these projections on the activity of the principal neurons of the dentate gyrus and CA3. The simulations show that uncorrelated input transmitted through the entorhinal-to-dentate or entorhinal-to-CA3 projection causes spatio-temporally correlated activity in the principal neurons that manifest as spike clusters. However, if the mossy fiber system provides uncorrelated input to the CA3, then the CA3 activity remains uncorrelated. When considering the transfer of correlation through the dentate, this analysis suggests that the mossy fiber system do not imbue any correlation to the activity as it propagates from the granule cells of the dentate to the CA3. With the spatio-temporal correlation analysis, the influence of each topographical projection on the transfer of correlation can be investigated as additional subregions and neuron types are added to the large-scale model.
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Hipocampo/fisiología , Modelos Neurológicos , Animales , Giro Dentado/fisiología , Canales Iónicos/metabolismo , Neuronas/fisiología , RatasRESUMEN
This paper describes a million-plus granule cell compartmental model of the rat hippocampal dentate gyrus, including excitatory, perforant path input from the entorhinal cortex, and feedforward and feedback inhibitory input from dentate interneurons. The model includes experimentally determined morphological and biophysical properties of granule cells, together with glutamatergic AMPA-like EPSP and GABAergic GABAA-like IPSP synaptic excitatory and inhibitory inputs, respectively. Each granule cell was composed of approximately 200 compartments having passive and active conductances distributed throughout the somatic and dendritic regions. Modeling excitatory input from the entorhinal cortex was guided by axonal transport studies documenting the topographical organization of projections from subregions of the medial and lateral entorhinal cortex, plus other important details of the distribution of glutamatergic inputs to the dentate gyrus. Results showed that when medial and lateral entorhinal cortical neurons maintained Poisson random firing, dentate granule cells expressed, throughout the million-cell network, a robust, non-random pattern of spiking best described as spatiotemporal "clustering". To identify the network property or properties responsible for generating such firing "clusters", we progressively eliminated from the model key mechanisms such as feedforward and feedback inhibition, intrinsic membrane properties underlying rhythmic burst firing, and/or topographical organization of entorhinal afferents. Findings conclusively identified topographical organization of inputs as the key element responsible for generating a spatio-temporal distribution of clustered firing. These results uncover a functional organization of perforant path afferents to the dentate gyrus not previously recognized: topography-dependent clusters of granule cell activity as "functional units" that organize the processing of entorhinal signals.
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Giro Dentado/fisiología , Modelos Neurológicos , Animales , Simulación por Computador , Dendritas/fisiología , Giro Dentado/citología , Corteza Entorrinal/fisiología , Hipocampo/fisiología , Red Nerviosa/fisiología , Neuronas/fisiología , Ratas , Agrupamiento Espacio-Temporal , Transmisión Sináptica , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico/metabolismo , Ácido gamma-Aminobutírico/metabolismoRESUMEN
The topography, or the anatomical connectivity, of the excitatory entorhinal-dentate-CA3 circuit of the rat hippocampus has been implemented for a large-scale, biologically realistic, computational model of the rat hippocampus. The implementation thus far covers only the excitatory synapses for the principal neurons in the hippocampal subregions. Starting from layer II of the entorhinal cortex, the projection of their perforant path axons has been mapped across the full extent of the dentate gyrus as well as to the CA3. The mossy fiber axon trajectories from the dentate granule cells to the CA3 pyramidal cells have been derived, incorporating the transverse route the fibers take through the CA3c and CA3b and the septo-temporal turn in the CA3a. The extensive arborization of the CA3 pyramidal axons have been modeled using 2-D, skewed Gaussian distributions which have been parametrized to exhibit the differences that exist among the CA3a, CA3b, and CA3c auto-associational projections. Using the limited samples available from the literature, key parameters for each projection have been interpolated as a function of transverse and/or septo-temporal position in order to create a more complete representation of the topography.
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Región CA3 Hipocampal/citología , Modelos Neurológicos , Algoritmos , Animales , Axones/fisiología , Giro Dentado/citología , Corteza Entorrinal/fisiología , Células Piramidales/fisiología , Ratas , Sinapsis/fisiologíaRESUMEN
A large-scale, biologically realistic, computational model of the rat hippocampus is being constructed to study the input-output transformation that the hippocampus performs. In the initial implementation, the layer II entorhinal cortex neurons, which provide the major input to the hippocampus, and the granule cells of the dentate gyrus, which receive the majority of the input, are modeled. In a previous work, the topography, or the wiring diagram, connecting these two populations had been derived and implemented. This paper explores the consequences of two features of the topography, the distribution of the axons and the size of the neurons' axon terminal fields. The topography converts streams of independently generated random Poisson trains into structured spatiotemporal patterns through spatiotemporal convergence achievable by overlapping axon terminal fields. Increasing the axon terminal field lengths allowed input to converge over larger regions of space resulting in granule activation across a greater area but did not increase the total activity as a function of time as the number of targets per input remained constant. Additional simulations demonstrated that the total distribution of spikes in space depends not on the distribution of the presynaptic axons but the distribution of the postsynaptic population. Analyzing spike counts emphasizes the importance of the postsynaptic distribution, but it ignores the fact that each individual input may be carrying unique information. Therefore, a metric should be created that relates and tracks individual inputs as they are propagated and integrated through hippocampus.
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Giro Dentado/ultraestructura , Modelos Biológicos , Análisis Espacio-Temporal , Animales , Axones/metabolismo , Simulación por Computador , Giro Dentado/fisiología , Corteza Entorrinal/fisiología , Corteza Entorrinal/ultraestructura , Neuronas/citología , Neuronas/fisiología , Neuronas/ultraestructura , RatasRESUMEN
In previously published work, we showed the progress we've made towards creating a large-scale, biologically realistic model of the rat hippocampus, starting with the projection from entorhinal cortex (EC) to the dentate gyrus (DG). We created the model to help us study how the common components of neurobiological systems in mammals - large numbers of neurons with intricate, branching morphologies; active, non-linear membrane properties; nonuniform distributions throughout membrane surface of these non-linear conductances; non-uniform and topographic connectivity between pre- and post-synaptic neurons; and activity-dependent changes in synaptic function - combine and contribute to give a particular brain region its "neural processing" properties. In this work, we report on the results of a series of simulations we ran to test the role of feed-forward and feedback inhibition in the dentate gyrus. We find that a) the system shows rhythmic bands of activity only in the presence of feedback inhibition, b) that the frequency of rhythmicity increases with increasing amounts of feed-forward inhibition, c) that it decreases with increasing amounts of feedback inhibition, and d) that strong excitatory inputs appear to enhance and prolong the amount of rhythmicity in the system.
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Hipocampo/citología , Hipocampo/fisiología , Modelos Neurológicos , Red Nerviosa/fisiología , Neuronas/citología , Animales , Corteza Entorrinal/fisiología , Red Nerviosa/citología , Neuronas/fisiología , RatasRESUMEN
Simultaneous stimulation of ex vivo pancreatic islets with dynamic oxygen and glucose is a critical technique for studying how hypoxia alters glucose-stimulated response, especially in transplant environments. Standard techniques using a hypoxic chamber cannot provide both oxygen and glucose modulations, while monitoring stimulus-secretion coupling factors in real-time. Using novel microfluidic device with integrated glucose and oxygen modulations, we quantified hypoxic impairment of islet response by calcium influx, mitochondrial potentials, and insulin secretion. Glucose-induced calcium response magnitude and phase were suppressed by hypoxia, while mitochondrial hyperpolarization and insulin secretion decreased in coordination. More importantly, hypoxic response was improved by preconditioning islets to intermittent hypoxia (IH, 1 min/1 min 5-21% cycling for 1 h), translating to improved insulin secretion. Moreover, blocking mitochondrial K(ATP) channels removed preconditioning benefits of IH, similar to mechanisms in preconditioned cardiomyocytes. Additionally, the multimodal device can be applied to a variety of dynamic oxygen-metabolic studies in other ex vivo tissues.