Hepatocellular carcinoma (HCC) is the main histological subtype of primary liver cancer and remains one of the most common solid malignancies globally. Ferroptosis was recently defined as an iron-catalyzed form of regulated necrosis. Because cancer cells exhibit higher iron requirements than noncancer cells, treatment with ferroptosis-inducing compounds may be a feasible strategy for cancer therapy. However, cancer cells develop acquired resistance to evade ferroptosis, and the mechanisms responsible for ferroptosis resistance are not fully clarified. In the current study, we reported that DDX39B was downregulated during sorafenib-induced ferroptosis in a dose- and time-dependent manner. Exogenous introduction of DDX39B ensured the survival of HCC cells upon exposure to sorafenib, while the opposite phenomenon was observed in DDX39B-silenced HCC cells. Mechanistically, we demonstrated that DDX39B increased GPX4 levels by promoting the splicing and cytoplasmic translocation of GPX4 pre-mRNA, which was sufficient to detoxify sorafenib-triggered excess lipid ROS production, lipid peroxidation accumulation, ferrous iron levels, and mitochondrial damage. Inhibition of DDX39B ATPase activity by CCT018159 repressed the splicing and cytoplasmic export of GPX4 pre-mRNA and synergistically assisted sorafenib-induced ferroptotic cell death in HCC cells. Taken together, our data uncover a novel role for DDX39B in ferroptosis resistance by modulating the maturation of GPX4 mRNA via a posttranscriptional approach and suggest that DDX39B inhibition may be a promising therapeutic strategy to enhance the sensitivity and vulnerability of HCC cells to sorafenib.
Tire-road characteristics are a critical focus of research in the automotive and transportation industries. On the one hand, the research can help optimize tires' structural design; on the other hand, it can analyze the mechanical response of the pavement structure under the vehicle load. In addition, the non-uniformity distribution of the tire ground stress will also have a direct impact on the skid resistance, which determines the driving safety. Due to the limitation of testing technology, the measurement of tire ground pressure was mainly carried out on a flat test platform, ignoring the roughness of the actual pavement surface texture. The tire-road contact characteristics research on the macro-texture and micro-texture of asphalt pavement needs to be broken through. A high-precision pressure-sensitive film measurement system is utilized to examine the actual contact characteristics between two types of automobile tires and three types of asphalt pavement in this paper. The influence law of pavement texture and patterned tires on the contact area and stress was explored, and the concentration effect of tire-road contact stress was evaluated. The results indicate that the contact area of grounding tires exhibits a nearly linear relationship with tire inflation pressure and load. Notably, the change in load has a more significant influence on the contact area than tire inflation pressure. On asphalt pavement, the contact reduction rate decreases by approximately 5-10% for block pattern tires and 10-15% for longitudinal pattern tires. Furthermore, as the texture depth of the pavement increases, the contact area between tires and the pavement texture decreases. The actual tire-road interface experiences significant stress concentration due to the embedding and meshing effects between the tire and road surface. Even on a flat steel surface, the peak stress at the edge of the tread block exceeds the 0.7 MPa design load, which is about 2.5-3 times higher than the design uniform load. The peak stress between the tire and asphalt pavement reaches 4-10 times the design uniform load, with a rising trend as the pavement texture depth increases. This study can provide relevant experimental technical support for tire design and functional design of asphalt pavement.
Stone matrix asphalt (SMA) mixture has been widely used in pavement engineering for its preferable in-service performance. However, deterioration of SMA pavement in skid resistance is apparent under traffic loading. There remains lacking attention on skid resistance attenuation of SMA pavement, which in turn is important for skid durability design in practice. Hence, this study aims to perform a thorough investigation to reveal the skid resistance attenuation law of SMA pavement. Multiple types of SMA-13 mixtures prepared by different material designs were selected to conduct a kneading test that simulates real surface states of in-service SMA pavement. Pressure-sensitive film and a 3D laser scanner were utilized for evaluating anti-skid performance and skid durability. The finite element (FE) method is introduced to simulate vehicle braking distance for skid-resistance evaluation. The results show that skid resistance attenuation of SMA pavement consists of two stages: In the first stage, the skid resistance of SMA pavement experiences a short enhancement, followed by a long-term weakening stage. Abundant surface texture of SMA helps to mitigate the impact of traffic load on skid resistance. The FE analysis and pressure-sensitive film results demonstrate the potential of skid durability design of SMA pavement based on the skid resistance attenuation law.
In order to improve the prediction accuracy regarding low compaction level of asphalt pavement, this paper carries out indoor tests to detect the voids and dielectric constants of AC-13, AC-16 and AC-25 asphalt mixtures, obtaining their relationship equations via linear fitting and determining the dielectric constant judgment threshold of low compaction level segregation risk points ε1. Based on the common mid-point method, three-dimensional ground-penetrating radar is used to obtain the dielectric constant of the physical engineering test section. The researcher can draw the distribution map of the low compaction level segregation risk area according to the judgment threshold ε1 of the rough segregation risk points; divide the connected risk areas; determine the regional convex hull; and calculate the regional indicators such as the regional area, the ratio of the convex risk points and the mean value of the regional dielectric constant. The response surface analysis method is used to acquire the model of risk area index and core void ratio. The model is employed to predict and verify the core void ratio in the risk area of the road section and verify the accuracy of the model. The results show that the error range between the predicted voids and the measured voids is -0.4%~+0.4%, and the mean absolute value of the error is 0.25%. Compared with the mean measured voids of 6.63%, the relative error is 3.77%, indicating that the model can accurately predict the regional low compaction level segregation degree.
Ultra-thin wearing course (UTWC) as an asphalt overlay is widely used in pavement maintenance for extending pavement service life. Researchers focused on improving and evaluating its performance, yet few researchers compare the performance of typical UTWCs. Moreover, some traditional asphalt mixture tests are improper for UTWC due to the thicknesses of UTWC, which is thinner than the traditional asphalt overlay. This study further evaluated the advantages and disadvantages of typical UTWCs. A series of tests were conducted to compare the comprehensive performance of three typical UWTC products, including SMA-10, Novachip-B, and GT-10. Moreover, this study improved the rutting test to evaluate its rutting performance more accurately. Rutting specimens of 20 mm thick and 50 mm thick composite specimens (20 mm UTWC + 30 mm Portland cement concrete slabs) were prepared. Two types of PCC slabs were used, including unprocessed PCC slabs and PCC slabs with preset cracks. The test results showed that Novachip-B showed the best water stability and weakest raveling resistance, while GT-10 showed the best fatigue and anti-skid performance. The rutting performance of UTWCs was reduced because of the influence of preset cracks. The rutting depth of GT-10 was only 60-90% of that of others, showing the comprehensive performance of GT-10 was better than that of others. These results provide a significant reference for the research and application of UTWC.
In the past three decades, researchers have engaged in the relationship between the composition, macro performance, and microstructure of asphalt. There are many research results in the use of atomic force microscopy (AFM) to study the microstructure and related mechanisms of asphalt. Based on previous studies, the performance of asphalt from its microstructure has been observed and analyzed, and different evaluation indices and modification methods have been proposed, providing guidance toward improving the performance of asphalt materials and benefiting potential applications. This review focuses on the typical application and analysis of AFM in the study of the aging regeneration and modification properties of asphalt. Additionally, this review introduces the history of the rheological and chemical testing of asphalt materials and the history of using AFM to investigate asphalt. Furthermore, this review introduces the basic principles of various modes of application of AFM in the microstructure of asphalt, providing a research direction for the further popularization and application of AFM in asphalt or other materials in the future. This review aims to provide a reference and direction for researchers to further popularize the application of AFM in asphalt and standardize the testing methods of AFM. This paper is also helpful in further exploring the relationship between the microstructure and macro performance of asphalt.
To mitigate excessively accumulated carbon dioxide (CO2) in the atmosphere and tackle the associated environmental concerns, green and effective approaches are necessary. The electrocatalytic CO2 reduction reaction (CO2RR) using sustainable electricity under benign reaction conditions represents a viable way to produce value-added and profitable chemicals. In this minireview, recent studies regarding unary Bi electrocatalysts and binary BiSn electrocatalysts are symmetrically categorized and reviewed, as they disclose high faradaic efficiencies toward the production of formate/formic acid, which has a relatively higher value of up to 0.50 $·per kg and has been widely used in the chemical and pharmaceutical industry. In particular, the preparation methodologies, electrocatalyst morphologies, catalytic performances and the corresponding mechanisms are comprehensively presented. The use of solid-state electrolytes showing high economic prospects for directly obtaining high-purity formic acid is highlighted. Finally, the remaining questions and challenges for CO2RR exploitations using Bi-related electrocatalysts are proposed, while perspectives and the corresponding strategies aiming to enhance their entire catalytic functionalities and boost their performance are provided.
Coarse aggregate is the main component of asphalt mixtures, and differences in its morphology directly impact road performance. The utilization of standard aggregates can benefit the standard design and performance improvement. In this study, 3D printing technology was adopted to prepare artificial aggregates with specific shapes for the purpose of making the properties of artificial aggregates to be similar to the properties of natural aggregates. Through a series of material experiments, the optimal cement-based material ratio for the preparation of high-strength artificial aggregates and corresponding manufacturing procedures have been determined. The performance of the artificial aggregates has been verified by comparing the physical and mechanical properties with those of natural aggregates. Results indicate that using 3D printing technology to generate the standard coarse aggregate is feasible, but its high cost in implementation cannot be ignored. The 3D shape of the artificial aggregate prepared by the grouting molding process has a good consistency with the natural aggregate, and the relative deviation of the overall macro-scale volume index of the artificial aggregate is within 4%. The average Los Angeles abrasion loss of artificial cement-based aggregate is 15.2%, which is higher than that of diabase aggregate, but significantly lower than that of granite aggregate and limestone aggregate. In a nutshell, 3D printed aggregates prepared using the optimized cement-based material ratio and corresponding manufacturing procedures have superior physical and mechanical performance, which provides technical support for the test standardization and engineering application of asphalt pavements.
Boron nitride (BN) aerogels are three-dimensional bulk materials with exceptional performances in a wide range of areas. However, detailed investigations into the relationship of synthesis, structure, and properties are rare. This study demonstrates the feasibility of tuning the performance of the aerogel by simply altering the relative amount of the precursors in the synthesis, which subsequently leads to the formation of aerogels with distinctive properties such as specific surface areas, porosity, and compressibility. The applications of these structurally different aerogels are exemplified by investigating in a series of important industrial-related areas, such as oil absorption/desorption, direct combustion, adsorptive desulfurisation, and CO2 capture. The study raises the application prospects of BN aerogels in gas-phase catalysis and thermal superinsulation materials.
In this article, two different types of spacer cations, 1,4-butanediamonium (BDA2+) and 2-phenylethylammonium (PEA+) are co-used to prepare the perovskite precursor solutions with the formula of (BDA)1- a (PEA2) a MA4Pb5X16. By simply mixing the two spacer cations, the self-assembled polycrystalline films of (BDA)0.8(PEA2)0.2MA4Pb5X16 are obtained, and BDA2+ is located in the crystal grains and PEA+ is distributed on the surface. The films display a small exciton binding energy, uniformly distributed quantum wells and improved carrier transport. Besides, utilizing mixed spacer cations also induces better crystallinity and vertical orientation of 2D perovskite (BDA)0.8(PEA2)0.2MA4Pb5X16 films. Thus, a power conversion efficiency (PCE) of 17.21% is achieved in the optimized perovskite solar cells with the device structure of ITO/PEDOT:PSS/Perovskite/PCBM/BCP/Ag. In addition, the complementary humidity and thermal stability are obtained, which are ascribed to the enhanced interlayer interaction by BDA2+ and improved moisture resistance by the hydrophobic group of PEA+. The encapsulated devices are retained over 95% or 75% of the initial efficiency after storing 500 h in ambient air under 40 ± 5% relative humidity or 100 h in nitrogen at 60 °C.
Lignin accounts for approximately 30% of the weight of herbaceous biomass. Utilizing lignin in asphalt pavement industry could enhance the performance of pavement while balancing the construction cost. This study aims to evaluate the feasibility of utilizing lignin as a bitumen performance improver. For this purpose, lignin derived from aspen wood chips (labeled as KL) and corn stalk residues (labeled as CL) were selected to prepare the lignin modified bituminous binder. The properties of the lignin modified binder were investigated through rheological, mechanical and chemical tests. The multiple stress creep recovery (MSCR) test results indicated that adding lignin decreased the Jnr of based binder by a range of 8% to 23% depending on the stress and lignin type. Lignin showed a positive effect on the low temperature performance of asphalt binder, because at -18 °C, KL and CL were able to reduce the stiffness of base binder from 441 MPa to 369 MPa and 378 MPa, respectively. However, lignin was found to deteriorate the fatigue life and workability of base binder up to 30% and 126%. With bituminous mixture, application of lignin modifiers improved the Marshall Stability and moisture resistance of base mixture up to 21% and 13%, respectively. Although, adding lignin modifiers decreased the molecular weight of asphalt binder according to the gel permeation chromatography (GPC) test results. The Fourier-transform infrared spectroscopy (FTIR) test results did not report detectable changes in functional group of based binder.
The permanent colouration of a polyester by straightforward azo coupling is disclosed. Uniquely, the chromophore is created only upon successful polymer modification with a non-coloured molecule (in situ colouration), which confirms successful polymer adaptation and ensures that coloured waste is not produced. The method of colouration, which may feasibly be applied for the coloration of a wide-range of step-growth polyesters, yielded a polymer capable of preventing indigo deposition onto a range of fabrics, offering potential use within advanced detergent formulations.
Azo Compounds/chemistry , Color , Polyesters/chemistry , Molecular Structure
The dual stimuli-controlled release of doxorubicin from gel-embedded nanoparticles is reported. Non-cytotoxic polymer nanoparticles are formed from poly(ethylene glycol)-b-poly(benzyl glutamate) that, uniquely, contain a central ester link. This connection renders the nanoparticles pH-responsive, enabling extensive doxorubicin release in acidic solutions (pHâ 6.5), but not in solutions of physiological pH (pHâ 7.4). Doxorubicin-loaded nanoparticles were found to be stable for at least 31â days and lethal against the three breast cancer cell lines tested. Furthermore, doxorubicin-loaded nanoparticles could be incorporated within a thermoresponsive poly(2-hydroxypropyl methacrylate) gel depot, which forms immediately upon injection of poly(2-hydroxypropyl methacrylate) in dimethyl sulfoxide solution into aqueous solution. The combination of the poly(2-hydroxypropyl methacrylate) gel and poly(ethylene glycol)-b-poly(benzyl glutamate) nanoparticles yields an injectable doxorubicin delivery system that facilities near-complete drug release when maintained at elevated temperatures (37 °C) in acidic solution (pHâ 6.5). In contrast, negligible payload release occurs when the material is stored at room temperature in non-acidic solution (pHâ 7.4). The system has great potential as a vehicle for the prolonged, site-specific release of chemotherapeutics.
Doxorubicin , Nanoparticles , Polymers/chemistry , Drug Delivery Systems , Drug Liberation , Humans , Hydrogen-Ion Concentration , Injections
The storage stability concern, caused by phase separation for the density difference between polymers and asphalt fractions, has limited the widespread application of polymer modified asphalt (PMA). Therefore, this study aims to improve the storage concern of PMA by incorporating nano-montmorillonite. To this end, different nano-montmorillonites were incorporated to three PMAs modified with three typical asphalt modifiers, i.e., crumb rubber (CRM), styrene-butadiene-rubber (SBR) and styrene-butadiene-styrene (SBS). A series of laboratory tests were performed to evaluate the storage stability and rheological properties of PMA binders with nano-montmorillonite. As a consequence, the incorporation of nano-montmorillonite exhibited a remarkable effect on enhancing the storage stability of the CRM modified binder, but limited positive effects for the SBR and SBS modified binders. The layered nano-montmorillonite transformed to intercalated or exfoliated structures after interaction with asphalt fractions, providing superior storage stability. Among selected nano-montmorillonites, the pure montmorillonite with Hydroxyl organic ammonium performed the best on enhancing storage stability of PMA. This paper suggests that nano-montmorillonite is a promising modifier to alleviate the storage stability concern for asphalt with polymer modifiers.
Quasi-2D Ruddlesden-Popper perovskites exhibit excellent photostability/environmental stability. However, the main drawback is their relatively low photovoltaic properties compared with three-dimensional perovskites. Herein, we demonstrated that chlorine-based additives via adjusting the proportion of PbI2 and PbCl2 in the precursor (BA)2(MA)3Pb4I13 (n = 4) solutions show an optimized device performance of over 15%, and the devices exhibit much improved humidity stability. Upon PbCl2 addition, the quasi-2D perovskites have larger and more compact grains, which result in high quality of films. The photoluminescence gives rise to a much prolonged lifetime under the PbCl2 additive, indicating fewer trap states to reduce the nonradiative recombination. The capacitance characteristics confirm that the PbCl2 additive can largely decrease the trap states in quasi-2D perovskite films. The capacitance-voltage characteristics indicate that using the PbCl2 additive decreases the charge accumulation toward increasing the charge collection in quasi-2D perovskite solar cells. Our work indicates that the addition of PbCl2 is an effective method to improve the device performance by reducing trap states and increasing charge collection toward developing high-performance quasi-2D perovskite devices.
As bituminous binders greatly influence the engineering performance of gussasphalt concrete for bridge deck pavement, selecting appropriate bitumen is a critical procedure for mixture design. In this study, five different combinations of bituminous binder for potential use in the "Hong Kong-Zhuhai-Macao Bridge" project were prepared and tested. To meet the strict requirements of quality control, a new Guss-Mastic Asphalt (GMA) system was developed. Three key indices, Lueer fluidity, impact toughness, and dynamic stability, were used for GMA design and construction quality control. The test results show that the fluidity of the GMA mixtures was affected by the shear thinning phenomenon. After mixing for three hours, the Lueer value of all GMA mixtures met the requirements of pouring construction. Moreover, it was found that the influence of mixing time on GMA with styrene butadiene styrene (SBS) modified bitumen was different to other prepared mixtures. This difference is ascribed to the degradation of SBS during the blending process at an elevated temperature. Finally, the blended bitumen (30% Pen60/70 + 70% Trinidad lake asphalt (TLA)) was applied to the project of the Hong Kong-Zhuhai-Macao Bridge.
Lignocellulosic biomass has gained increasing attention as a performance modifier for bituminous material due to the vast amount available, its low cost and its potential to improve the durability of pavement. However, a comprehensive study concerning both the binder and mixture performance of modified bituminous material with lignocellulose is still limited. This research aims to evaluate the feasibility of applying lignocellulose as bitumen modifier by rheological, chemical and mechanical tests. To this end, two lignocellulosic biomass modified bituminous binders and corresponding mixtures were prepared and tested. The chemical characterization revealed the interaction between lignocellulosic biomass and bitumen fractions. Rheological test results have shown that lignocellulosic modifiers improve the overall performance of bituminous binder at high, intermediate and low temperatures. The findings obtained by mixture mechanical tests were identical to the binder test results, proving the positive effect of lignocellulosic biomass on overall paving performance of bituminous materials. Although lignocellulosic modifier slightly deteriorates the bitumen workability, the modified bitumen still meets the viscosity requirements mentioned in Superpave specification. This paper suggests that lignocellulosic biomass is a promising modifier for bituminous materials with both engineering and economic merits. Future study will focus on field validation and life cycle assessment of bituminous pavement with lignocellulosic biomass.
Asphalt rubber (AR) is a sustainable paving material with merits including waste tire consumption, low traffic noise, and enhanced mechanical performance. However, the poor workability and storage stability limited its further application. This study attempted to alleviate these two concerns of AR simultaneously by incorporating heavy bio oil (HBO). To achieve this goal, bio-AR binders with three different mixing sequences were prepared. A series of rheological and chemical tests were conducted. Test results prove that the bio-AR binders exhibited superior rutting and fatigue resistance compared to AR binder. The viscosity values of bio-ARs were closed to AR modified with commercial warm mix additive, which indicates enhancement in workability. Due to the relatively high density of HBO, the density difference between the asphalt liquid phase and crumb rubber in the bio-AR system narrowed, which brought improved storage stability. Among bio-ARs prepared with different mixing sequences, the direct mixing one (ARB) had the most satisfied overall performance. The early incorporation of HBO had limited negative influence on binder performance, but allowed for more energy saving during the bio-AR binder production. Future study will be conducted on performance of bio-AR mixtures and quantitative estimation of its energy saving during the blending and compacting process.
Oxidative stress caused by free radicals is one of the great threats to inflict intracellular damage. Here, we report a convenient approach to the synthesis, characterization, and evaluation of the radical activity of titanium-based composites. We have investigated the potential of natural antioxidants (curcumin, quercetin, catechin, and vitamin E) as radical scavengers and stabilizers. The titanium oxide composites were prepared via three steps including sol-gel synthesis, carboxylation, and esterification. The characterization of the titanium-phenol composites was carried out by FTIR, PXRD, UV-vis and SEM methods. The radical scavenging ability of the novel materials was evaluated using DPPH and an in vitro LPO assay using isolated rat liver mitochondria. The novel materials exhibit both a higher stability and an antioxidant activity in comparison to bare TiO2. It was found that curcumin and quercetin based composites show the highest antioxidant efficiency among the composites under study followed by catechin and vitamin E based materials. The results from an MTT assay carried out on the Caco-2 cell line indicate that the composites do not contribute to the cytotoxicity in vitro. This study demonstrates that a combination of powerful antioxidants with titanium dioxide can change its functional properties and provide a convenient strategy against oxidative stress.
