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Carboxymethyl cellulose (CMC) is a well-known cellulose derivative used in biomedical applications due to its biocompatibility and biodegradability. In this work, novel porous CMC materials, aerogels, were prepared and tested as a drug delivery device. CMC aerogels were made from CMC solutions, followed by non-solvent induced phase separation and drying with supercritical CO2. The influence of CMC characteristics and of processing conditions on aerogels' density, specific surface area, morphology and drug release properties were investigated. Freeze-drying of CMC solutions was also used as an alternative process to compare the properties of the as-obtained "cryogels" with those of aerogels. Aerogels were nanostructured materials with bulk density below 0.25 g/cm3 and high specific surface area up to 143 m2/g. Freeze drying yields highly macroporous materials with low specific surface areas (around 5-18 m2/g) and very low density, 0.01 - 0.07g/cm3. Swelling and dissolution of aerogels and cryogels in water and in a simulated wound exudate (SWE) were evaluated. The drug was loaded in aerogels and cryogels, and release kinetics in SWE was investigated. Drug diffusion coefficients were correlated with material solubility, morphology, density, degree of substitution and drying methods, demonstrating tuneability of new materials' properties in view of their use as delivery matrices.
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Carboximetilcelulosa de Sodio , Criogeles , Geles , Sistemas de Liberación de Medicamentos , CelulosaRESUMEN
Ulva prolifera macroalgae blooming caused by water eutrophication seriously affects the marine ecological environment. Exploring an efficient approach to turning algae biomass waste into high-value-added products is significant. The present work aimed to demonstrate the feasibility of the bioactive polysaccharide extraction from Ulva prolifera and to evaluate its potential biomedical application. A short autoclave process was proposed and optimized using the response surface methodology to extract Ulva polysaccharides (UP) with high molar mass. Our results indicated that UP with high molar mass (9.17 × 105 g/mol) and competitive radical scavenging activity (up to 53.4 %) could be effectively extracted with the assistance of Na2CO3 (1.3 %, wt.) at a solid-liquid ratio of 1/10 in 26 min. The obtained UP mainly composes of galactose (9.4 %), glucose (73.1 %), xylose (9.6 %), and mannose (4.7 %). The biocompatibility of the UP and its potential application as a bioactive ingredient in 3D cell culture has been evaluated and confirmed by confocal laser scanning microscopy and fluorescence microscope imaging inspection. This work demonstrated the feasibility of extracting bioactive sulfated polysaccharides with potential applications in biomedicine from biomass waste. Meanwhile, this work also provided an alternative solution to deal with the environmental challenges incurred by algae blooming worldwide.
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Algas Marinas , Ulva , Biomasa , Polisacáridos/farmacología , GlucosaRESUMEN
Nanocellulose has great potential in the biomedical field due to its biocompatibility, large specific surface area, and customizable surface chemistry. However, due to the bioinert nature and mismatch of the mechanical strength, nanocellulose itself has no cell adhesion ability and cannot directly promote cell growth and reproduction. Recently, surface functionalization of nanocellulose has been reported as an indispensable strategy for improving its bioactivities or other physic-chemical properties. In this paper, functionalization strategies of nanocellulose based on its inherent hydroxyl, aldehyde, carboxyl, and sulfate group reactions are reviewed. Biomacromolecules, such as peptides, proteins, and DNA that are commonly used in functionalization for different biomedical applications are summarized. Prospects and ongoing challenges of nanocellulose-based biomaterials application, as well as these advanced processing technologies such as additive manufacturing, nanomanufacturing, and bio-manufacturing are also discussed. This review is supposed to serve as a guideline for the development of nanocellulose-based biomaterials in biomedical applications.
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Celulosa , Nanoestructuras , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Celulosa/química , Nanoestructuras/químicaRESUMEN
Excessive bleeding in traumatic hemorrhage is the primary concern for natural wound healing and the main reason for trauma deaths. The three-dimensional (3D) bioprinting of bioinks offers the desired structural complexity vital for hemostasis activity and targeted cell proliferation in rapid and controlled wound healing. However, it is challenging to develop suitable bioinks to fabricate specific 3D scaffolds desirable in wound healing. In this work, a 3D composite scaffold is designed using bioprinting technology and synergistic hemostasis mechanisms of cellulose nanofibrils (TCNFs), chitosan, and casein to control blood loss in traumatic hemorrhage. Bioinks that consist of casein bioconjugated TCNF (with a casein content of 104.5 ± 34.1 mg/g) using the carbodiimide cross-linker chemistry were subjected to bioprinting for customizable 3D scaffold fabrication. Further, the 3D composite scaffolds were in situ cross-linked using a green ionic complexation approach. The covalent conjugation among TCNF, casein, and chitosan was confirmed by Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and X-ray diffraction (XRD) studies. The in vitro hemostasis activity of the 3D composite scaffold was analyzed by a human thrombin-antithrombin (TAT) assay and adsorption of red blood cells (RBCs) and platelets. The 3D composite scaffold had a better swelling behavior and a faster whole blood clotting rate at each time point than the 3D TCNF scaffold and commercial cellulose-based dressings. The TAT assay demonstrated that the 3D composite scaffold could form a higher content of thrombin (663.29 pg/mL) and stable blood clot compared to a cellulosic pad (580.35 pg/mL), 3D TCNF (457.78 pg/mL), and cellulosic gauze (328.92 pg/mL), which are essential for faster blood coagulation. In addition, the 3D composite scaffold had a lower blood clotting index (23.34%) than the 3D TCNF scaffold (41.93%), suggesting higher efficiencies for RBC entrapping to induce blood clotting. The in vivo cytocompatibility was evaluated by a 3D cell culture study, and results showed that the 3D composite scaffold could promote growth and proliferation of NIH 3T3 fibroblast cells, which is vital for wound healing. Cellulase-based in vitro deconstruction of the 3D composite scaffold showed significant weight loss (80 ± 5%) compared to the lysozyme hydrolysis (22 ± 5%) after 28 days of incubation, suggesting the biodegradation potential of the composite scaffold. In conclusion, this study proposes efficient prospects to develop a 3D composite scaffold from bioprinting of TCNF-based bioinks that can accelerate blood clotting and wound healing, suggesting its potential application in reducing blood loss during traumatic hemorrhage.
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Materiales Biocompatibles/farmacología , Caseínas/farmacología , Celulosa/farmacología , Nanofibras/química , Impresión Tridimensional , Cicatrización de Heridas/efectos de los fármacos , Materiales Biocompatibles/química , Caseínas/química , Celulosa/química , Humanos , Ensayo de Materiales , Andamios del Tejido/químicaRESUMEN
Biomass waste comes from a wide range of sources, such as forest, agricultural, algae wastes, as well as other relevant industrial by-products. It is an important alternative energy source as well as a unique source for various bioproducts applied in many fields. For the past two decades, how to reuse, recycle and best recover various biomass wastes for high value-added bioproducts has received significant attention, which has not only come from various academia communities but also from many civil and medical industries. To summarize one of the cutting-edge technologies applied with nanocellulose biomaterials, this review focused on various preparation methods and strategies to make nanocellulose from diverse biomass wastes and their potential applications in biomedical areas and other promising new fields.
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3D printing enables the complex or customized structures production in high speed and resolution. However, the lack of bio-based materials with user-defined biochemical and mechanical property is a significant barrier that limits the widespread adoption of 3D printing for products fabrication. Development of eco-friendly natural-derived biopolymers for 3D printing technologies and their promising application in different areas are of huge academic, and environmental interests. This paper reviews the state-of-the-art in terms of 3D printing technology using natural-derived feedstocks, including lignocellulose, starch, algae, and chitosan-based biopolymers. Special consideration is given to the development of lignocellulosic materials, i.e. cellulose, hemicellulose, lignin, and their derivatives as 3D printing feedstocks. A strategical development roadmap with identified material property requirements, key challenges, as well as possible solutions was proposed. It serves as guideline aiming to explore natural-derived biopolymers as novel feedstocks for different 3D printing technologies that will be potentially applied in various areas.