[Research advances in drug resistance mechanisms of bacterial biofilm and natural drug intervention].

Drug resistance resulting from bacterial biofilms can invalidate antibacterial agents. Therefore,eradicating bacterial biofilms to reverse drug resistance is a hotspot in the pharmaceutical research. In recent years,numerous studies have revealed the complicated mechanism of bacterial biofilm formation and strong drug resistance with multiple influential factors involved. This paper gives a comprehensive review on the process of biofilm formation and intervention by natural drugs,which can provide some reference and evidence for the following studies.

Determination of two main components in bark of Paeonia suffruticosa by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.</p><p><b>METHOD</b>The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.</p><p><b>RESULT</b>The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.</p><p><b>CONCLUSION</b>The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.</p>