Differences in psychological distress, resilience and cognitive emotional regulation strategies in adults during the Coronavirus pandemic: A cross-cultural study of Poland and Spain

El objetivo de este estudio transcultural fue analizar las diferencias en resiliencia, estrategias de regulación cognitiva de las emociones (CERS) y distress psicológico durante la pandemia del COVID-19 en Polonia y España. Método. Se realizó una encuesta online en una muestra de 1,182 adultos. Se llevó a cabo un análisis MANOVA para examinar las diferencias en las variables estudiadas entre estos países. Esto fue seguido de un análisis MANCOVA controlando el sexo y la edad. Se realizaron análisis de regresión lineal segmentados por país con el fin de identificar modelos predictivos de distrés psicológico. Resultados. La población polaca se caracterizó por niveles más altos de ansiedad, menos resiliencia y más uso de CERS desadaptativas durante el confinamiento. La población española sufrió más estrés pero utilizó CERS adaptativas y fue más resiliente. La edad y el sexo femenino aparecieron como factores de riesgo de malestar psicológico en España. Se encontró que los modelos de predicción de distrés psicológico fueron diferentes en ambos países: las CERS adaptativas fueron predictivas en España y la resiliencia fue predictiva en Polonia. Conclusiones. Este estudio podría guiar en iniciativas para la promoción del bienestar psicológico como vía para prevenir trastornos psicopatológicos durante la pandemia.(AU)

Background.The objective of this cross-cultural study was to ana-lyze the differences in resilience, cognitive emotion regulation strategies (CERS), as well as psychological distress during the COVID-19 pandemic between Poland and Spain. Method.An online survey was conducted on a sample of 1,182 adults. A MANOVA analysis was carried out to examine the differences in the studied variables between these countries. This was followed by MANCOVA analysis to control for gender and age. Linear re-gression analyses segmented by country were conducted in order to identi-fy psychological distress prediction models. Results.Polish population was characterized by higher levels of anxiety, less resilience and more maladap-tive CERS during the lockdown. Spanish population was more affected by stress but used more adaptable CERS and was more resilient. Age and fe-male gender appeared as risk factors of psychological distress in Spain. The psychological distress prediction models were found to be different be-tween both countries: adaptive CERS was predictive only in Spain, and re-silience was predictive only in Poland. Conclusions.This study could guide in initiatives for the promotion of psychological well-being as a way to pre-vent psychopathological disorders during the pandemic.(AU)

Personal Variables of Protection against Cannabis Use in Adolescence: The Roles of Emotional Intelligence, Coping Styles, and Assertiveness as Associated Factors.

(1) Background: Many public bodies have warned of the increased consumption of cannabis, particularly among adolescents. From the Positive Youth Development approach, the promotion of personal protective factors takes on special importance against some risks, such as the consumption of addictive substances. (2) Methods: This research is one of the first to study the role of trait and ability emotional intelligence in relation to cannabis use and with respect to other personal variables of protection, such as coping styles and assertiveness. For this purpose, a final sample of 799 schoolchildren was obtained. (3) Results: After controlling for age and gender, the results of the regression analyses revealed that emotional perception, emotional facilitation, emotional clarity, emotional repair, active coping style, and assertiveness were inversely and significantly associated with cannabis use behaviors. On the other hand, the emotional attention and avoidant coping style factors were positively and significantly associated with these behaviors. (4) Conclusions: These findings provide new evidence that could be useful in terms of guiding health-promoting clinical and educational interventions at an early age.

¿Qué Aporta la Inteligencia Emocional al Estudio de los Factores Personales Protectores del Consumo de Alcohol en la Adolescencia? / How does emotional intelligence contribute to the study of personal protective factors for alcohol consumption in adolescence?

Diversos organismos alertan sobre el incremento del consumo de alcohol y de su uso extendido en los adolescentes. Algunas investigaciones dan cuenta de la relevancia que posee la inteligencia emocional (IE) como nuevo constructo que puede ayudar a explicar esta problemática. El objetivo de este estudio es analizar la contribución de la IE rasgo y habilidad a la comprensión del consumo de alcohol respecto a otros factores protectores personales previamente estudiados como la autoestima, la autoeficacia y la asertividad. Esta investigación desarrolla una metodología de corte cuantitativo y de tipo correlacional en una muestra de 799 estudiantes. Los resultados revelan que los factores de percepción emocional, claridad emocional, reparación emocional, autoestima y asertividad se asociaron de manera significativa e inversamente con las conductas de consumo de alcohol, al contrario que el componente de atención emocional. Estos hallazgos proporcionan nueva evidencia empírica que podría orientar intervenciones preventivas a edades tempranas

A lot of public bodies have warned against an increase in alcohol intake and its extended use among adolescents. Some studies have revealed the relevance of emotional intelligence (EI) as a novel construct that may be useful in explaining this problem. The objective of this study is to analyze the contribution of trait and ability EI to the understanding of alcohol consumption with respect to other personal protective factors such as self-esteem, self-efficacy, and assertiveness previously studied. A correlational and quantitative methodology was used on a sample of 799 students. The results suggest that the factors of emotional perception, emotional clarity, emotional repair, self-esteem, and assertiveness were significantly and inversely related to alcohol consumption behaviors, unlike the emotional attention component. These findings offer new empirical evidence that may assist in establishing preventive interventions targeting young people

Cognitive emotion regulation strategies mediate the relationships between Dark Triad traits and negative emotional states experienced during the COVID-19 pandemic.

COVID-19 has become a major source of stress as it puts individuals at risk of a range of mental health problems. Personality traits may predispose people to use adaptive or maladaptive coping strategies that lead to different health-related outcomes. The goal of the present study was to examine whether the use of distinct coping strategies during this stressful COVID-19 outbreak mediates the relationships between Dark Triad (DT) traits and stress, depression, and anxiety. The study was conducted in Poland (N = 1086) and Spain (N = 582), thus cross-culturally validated measures were used to assess depression, anxiety and stress (DASS-21), cognitive emotion regulation strategies (CERQ) and socially aversive traits covered by DT (Dirty Dozen scale). The study shows that maladaptive CERS mediates the relationships between narcissism/Machiavellianism and stress, anxiety and depression. Additionally, adaptive CERS mediates the relationship between psychopathy and depression. The results provide a better understanding of the mediating role of CERS on the relationships between DT traits and the stress, anxiety and depression experienced during the COVID-19 pandemic.

Trait and ability emotional intelligence as factors associated with cannabis use in adolescence. / Inteligencia emocional rasgo y habilidad como factores asociados al consumo de cannabis en la adolescencia.

Many international organisms have warned of the increased consumption of cannabis and its extensive use by adolescents. This study is one of the first with the aim of analyzing the role of ability and trait emotional intelligence, based on the model of Mayer and Salovey, with regards to the consumption of cannabis by adolescents. The study participants were 799 Spanish nationals aged 12 to 16. They were administered a self-report on trait emotional intelligence (EI), a test of maximum EI performance and were asked about their habits relating to cannabis consumption. This cross-sectional study used a quantitative, correlational methodology. The main results obtained from the regression analysis once gender, age and context of residence were controlled for, revealed negative associations between the factors of understanding and emotional repair of trait EI and the cannabis consumption variables, in contrast to emotional attention. On the other hand, with regards to ability EI, the factors of perception and facilitation were inversely associated with cannabis consumption in adolescents. The results suggest that both trait and ability EI are complementary constructs that help to explain cannabis consumption during this life stage. These findings offer empirical evidence that may help guide clinical and educational interventions focused on prevention of consumption during this period.

Diversos organismos internacionales alertan sobre el incremento de consumo de cannabis y de su uso extendido entre los adolescentes. El presente estudio ha sido uno de los primeros con el objetivo de analizar el papel de la inteligencia emocional rasgo y habilidad, basada en el modelo de Mayer y Salovey, en relación al consumo de cannabis en adolescentes. En este estudio participaron 799 jóvenes españoles con edades comprendidas entre los 12 y los 16 años. Se administró un autoinforme de inteligencia emocional (IE) rasgo, un test de rendimiento máximo de IE y se preguntó sobre los hábitos relacionados con el consumo de cannabis. Este estudio de tipo transversal se llevó a cabo a través de una metodología de corte cuantitativo y de tipo correlacional. Los principales resultados obtenidos mediante los análisis de regresión una vez controlados el género, la edad y el contexto de centro, revelaron asociaciones negativas entre los factores de comprensión y reparación emocional de la IE rasgo y las variables de consumo de cannabis, al contrario que la atención emocional. Por otro lado, en relación con la IE habilidad, los factores de percepción y facilitación se asociaron de manera inversa al consumo de cannabis en los adolescentes. Los resultados de este estudio sugieren que tanto la IE rasgo como la IE habilidad son constructos complementarios que ayudan a explicar el consumo de cannabis. Estos hallazgos proporcionan evidencias empíricas que podrían orientar intervenciones clínicas y educativas enfocadas a la prevención del consumo en esta etapa.

Inteligencia emocional rasgo y habilidad como factores asociados al consumo de cannabis en la adolescencia / Trait and ability emotional intelligence as factors associated with cannabis use in adolescence

Diversos organismos internacionales alertan sobre el incremento deconsumo de cannabis y de su uso extendido entre los adolescentes.El presente estudio ha sido uno de los primeros con el objetivo deanalizar el papel de la inteligencia emocional rasgo y habilidad, basada en el modelo de Mayer y Salovey, en relación al consumo decannabis en adolescentes. En este estudio participaron 799 jóvenesespañoles con edades comprendidas entre los 12 y los 16 años. Seadministró un autoinforme de inteligencia emocional (IE) rasgo, untest de rendimiento máximo de IE y se preguntó sobre los hábitosrelacionados con el consumo de cannabis. Este estudio de tipo transversal se llevó a cabo a través de una metodología de corte cuantitativoy de tipo correlacional. Los principales resultados obtenidos mediantelos análisis de regresión una vez controlados el género, la edad y elcontexto de centro, revelaron asociaciones negativas entre los factoresde comprensión y reparación emocional de la IE rasgo y las variablesde consumo de cannabis, al contrario que la atención emocional. Porotro lado, en relación con la IE habilidad, los factores de percepción yfacilitación se asociaron de manera inversa al consumo de cannabis enlos adolescentes. Los resultados de este estudio sugieren que tanto laIE rasgo como la IE habilidad son constructos complementarios queayudan a explicar el consumo de cannabis. Estos hallazgos proporcionan evidencias empíricas que podrían orientar intervenciones clínicas y educativas enfocadas a la prevención del consumo en esta etapa. (AU)

Many international organisms have warned of the increased consumption of cannabis and its extensive use by adolescents. Thisstudy is one of the first with the aim of analyzing the role of abilityand trait emotional intelligence, based on the model of Mayer andSalovey, with regards to the consumption of cannabis by adolescents.The study participants were 799 Spanish nationals aged 12 to 16.They were administered a self-report on trait emotional intelligence(EI), a test of maximum EI performance and were asked about theirhabits relating to cannabis consumption. This cross-sectional studyused a quantitative, correlational methodology. The main resultsobtained from the regression analysis once gender, age and context ofresidence were controlled for, revealed negative associations betweenthe factors of understanding and emotional repair of trait EI and thecannabis consumption variables, in contrast to emotional attention.On the other hand, with regards to ability EI, the factors of perceptionand facilitation were inversely associated with cannabis consumptionin adolescents. The results suggest that both trait and ability EI arecomplementary constructs that help to explain cannabis consumptionduring this life stage. These findings offer empirical evidence thatmay help guide clinical and educational interventions focused onprevention of consumption during this period. (AU)

Empatía, estilos de afrontamiento y actitudes hacia el consumo como factores de protección del consumo intensivo de alcohol y el policonsumo en la adolescencia / Empathy, coping styles and attitudes towards consumption as protective factors for heavy alcohol consumption and polydrug use in adolescence

Desde el enfoque del desarrollo positivo adolescente cobra especial importancia la promoción de recursos personales de protección frente a algunos riesgos como el consumo de drogas. El objetivo de este estudio fue analizar la influencia de la empatía, los estilos de afrontamiento y las actitudes hacia el consumo respecto al uso intensivo de alcohol y el policonsumo en 799 estudiantes. Los resultados del análisis de regresión revelaron que el afrontamiento improductivo predijo un mayor consumo intensivo de alcohol, mientras que la actitud de rechazo ante el ofrecimiento de alcohol y la actitud de admiración hacia personas no usuarias de drogas institucionalizadas se asociaron con un menor hábito de consumo. Con relación al policonsumo, tanto la empatía afectiva, como el afrontamiento activo, la actitud contraria y de rechazo al consumo de drogas, así como la actitud de admiración hacia personas no usuarias de drogas institucionalizadas predijeron un menor uso simultaneo de alcohol y cannabis. Estos hallazgos aportan nuevas evidencias que podrían ser de utilidad para orientar intervenciones promotoras de la salud a edades tempranas (AU)

From a positive youth development perspective, the promotion of personal protective resources against certain risks such as drug consumption is especially relevant. The objective of this study was to analyze the influence of empathy, coping styles, and attitudes related to consumption with respect to binge drinking and polyconsumption in a sample of 799 students. The results of a regression analysis revealed that unproductive coping predicted binge drinking, while attitudes such as turning down a drink and admiration of non-users of institutionalized drugs were associated with a lower consumption habit. As for polyconsumption, affective empathy, active coping, a negative attitude to, and the rejection of drug consumption, as well as the admiration of non-users of institutionalized drugs, predicted a lower simultaneous use of alcohol and cannabis. These findings offer new evidence that may be useful in guiding interventions to promote healthy habits at early ages (AU)

The Protective Association of Trait and Ability Emotional Intelligence with Adolescent Tobacco Use.

The present study is one of the first to analyze the predictive capacity of both trait and ability Emotional Intelligence (EI) based on the Mayer and Salovey model, in relation to tobacco use in a sample of Spanish adolescents. In this study, 799 students between the ages of 12 and 16 participated. A self-report on trait EI, an EI peak performance test, and questions about habits relating to tobacco use were administered. This cross-sectional study developed a quantitative and correlation-type methodology. The main results of the regression analyses, once the sex and age of the participants were controlled, revealed negative associations between the factors of clarity and emotional repair of the trait EI with respect to the variables of tobacco use, and a positive association was found for them and emotional attention. By comparison, with respect to ability EI, emotional perception and understanding were inversely related to adolescent tobacco use. These results underscore the importance of EI skills as protective factors against early initiation and subsequent tobacco abuse.

Trait and ability emotional intelligence as predictors of alcohol consumption in adolescents / Inteligencia Emocional rasgo y habilidad como predictores del consumo de alcohol en la adolescencia

Background: Past studies have suggested that emotional intelligence (EI) is a new construct that may help to explain alcohol abuse. This study is one of the first to examine the predictive capacity of both trait and ability EI, based on the Mayer and Salovey model, with regards to distinct variables of alcohol consumption in an adolescent population. Method: A survey was conducted on 844 school children who completed a self-report and performance-based EI test, as well as a selection of items on alcohol consumption. Results: Regression analyses revealed that trait EI was the most predictive. Clarity and emotional Repair showed significant negative correlations with alcohol consumption, as opposed to emotional Attention. Likewise, the abilities of Perceiving and Using emotions were found to be protective factors for consumption in schoolchildren. Conclusion: The results suggest that trait and ability EI are complementary dimensions that may help in the understanding and intervention of alcohol abuse in adolescents. The results are discussed in the EI research context, as well as their educational implications and future research lines

Antecedentes: diversas investigaciones muestran que la inteligencia emocional (IE) es un nuevo constructo que puede ayudar a explicar el consumo abusivo de alcohol. El presente estudio es uno de los primeros en examinar la capacidad predictiva de la IE rasgo y de la IE habilidad, basada en el modelo de Mayer y Salovey, sobre diversas variables de consumo de alcohol en población adolescente. Método: se realizó una encuesta a 844 escolares que cumplimentaron un autoinforme y un test de rendimiento máximo de IE, así como una selección de ítems de consumo de alcohol. Resultados: los análisis de regresión revelaron que la IE rasgo fue la más predictiva. La Claridad y la Reparación emocional mostraron relaciones significativas negativas con el consumo de alcohol, al contrario que la Atención emocional. Asimismo, la habilidad de Percepción y Facilitación emocional resultaron ser factores protectores del consumo en los escolares. Conclusión: los resultados sugieren que la IE rasgo y habilidad son medidas complementarias que pueden ayudar en la comprensión e intervención sobre el problema del abuso del alcohol en la adolescencia. Se discuten los resultados en el contexto de la investigación sobre la IE, así como su implicación educativa y futuras líneas de investigación

Trait and ability emotional intelligence as predictors of alcohol consumption in adolescents.

BACKGROUND: Past studies have suggested that emotional intelligence (EI) is a new construct that may help to explain alcohol abuse. This study is one of the first to examine the predictive capacity of both trait and ability EI, based on the Mayer and Salovey model, with regards to distinct variables of alcohol consumption in an adolescent population. METHOD: A survey was conducted on 844 school children who completed a self-report and performance-based EI test, as well as a selection of items on alcohol consumption. RESULTS: Regression analyses revealed that trait EI was the most predictive. Clarity and emotional Repair showed significant negative correlations with alcohol consumption, as opposed to emotional Attention. Likewise, the abilities of Perceiving and Using emotions were found to be protective factors for consumption in schoolchildren. CONCLUSION: The results suggest that trait and ability EI are complementary dimensions that may help in the understanding and intervention of alcohol abuse in adolescents. The results are discussed in the EI research context, as well as their educational implications and future research lines.

High fat diet blunts the effects of leptin on ventilation and on carotid body activity.

KEY POINTS: Leptin plays a role in the control of breathing, acting mainly on central nervous system; however, leptin receptors have been recently shown to be expressed in the carotid body (CB), and this finding suggests a physiological role for leptin in the regulation of CB function. Leptin increases minute ventilation in both basal and hypoxic conditions in rats. It increases the frequency of carotid sinus nerve discharge in basal conditions, as well as the release of adenosine from the CB. However, in a metabolic syndrome animal model, the effects of leptin in ventilatory control, carotid sinus nerve activity and adenosine release by the CB are blunted. Although leptin may be involved in triggering CB overactivation in initial stages of obesity and dysmetabolism, resistance to leptin signalling and blunting of responses develops in metabolic syndrome animal models. ABSTRACT: Leptin plays a role in the control of breathing, acting mainly on central nervous system structures. Leptin receptors are expressed in the carotid body (CB) and this finding has been associated with a putative physiological role of leptin in the regulation of CB function. Since, the CBs are implicated in energy metabolism, here we tested the effects of different concentrations of leptin administration on ventilatory parameters and on carotid sinus nerve (CSN) activity in control and high-fat (HF) diet fed rats, in order to clarify the role of leptin in ventilation control in metabolic disease states. We also investigated the expression of leptin receptors and the neurotransmitters involved in leptin signalling in the CBs. We found that in non-disease conditions, leptin increases minute ventilation in both basal and hypoxic conditions. However, in the HF model, the effect of leptin in ventilatory control is blunted. We also observed that HF rats display an increased frequency of CSN discharge in basal conditions that is not altered by leptin, in contrast to what is observed in control animals. Leptin did not modify intracellular Ca2+ in CB chemoreceptor cells, but it produced an increase in the release of adenosine from the whole CB. We conclude that CBs represent an important target for leptin signalling, not only to coordinate peripheral ventilatory chemoreflexive drive, but probably also to modulate metabolic variables. We also concluded that leptin signalling is mediated by adenosine release and that HF diets blunt leptin responses in the CB, compromising ventilatory adaptation.

Hypoxic pulmonary vasoconstriction, carotid body function and erythropoietin production in adult rats perinatally exposed to hyperoxia.

KEY POINTS: Adult animals that have been perinatally exposed to oxygen-rich atmospheres (hyperoxia), recalling those used for oxygen therapy in infants, exhibit a loss of hypoxic pulmonary vasoconstriction, whereas vasoconstriction elicited by depolarizing agents is maintained. Loss of pulmonary hypoxic vasoconstriction is not linked to alterations in oxygen-sensitive K(+) currents in pulmonary artery smooth muscle cells. Loss of hypoxic vasoconstriction is associated with early postnatal oxidative damage and corrected by an antioxidant diet. Perinatal hyperoxia damages carotid body chemoreceptor cell function and the antioxidant diet does not reverse it. The hypoxia-elicited increase in erythropoietin plasma levels is not affected by perinatal hyperoxia. The potential clinical significance of the findings in clinical situations such as pneumonia, chronic obstructive pulmonary disease or general anaesthesia is considered. ABSTRACT: Adult mammalians possess three cell systems that are activated by acute bodily hypoxia: pulmonary artery smooth muscle cells (PASMC), carotid body chemoreceptor cells (CBCC) and erythropoietin (EPO)-producing cells. In rats, chronic perinatal hyperoxia causes permanent carotid body (CB) atrophy and functional alterations of surviving CBCC. There are no studies on PASMC or EPO-producing cells. Our aim is to define possible long-lasting functional changes in PASMC or EPO-producing cells (measured as EPO plasma levels) and, further, to analyse CBCC functional alterations. We used 3- to 4-month-old rats born and reared in a normal atmosphere or exposed to perinatal hyperoxia (55-60% O2 for the last 5-6 days of pregnancy and 4 weeks after birth). Perinatal hyperoxia causes an almost complete loss of hypoxic pulmonary vasoconstriction (HPV), which was correlated with lung oxidative status in early postnatal life and prevented by antioxidant supplementation in the diet. O2 -sensitivity of K(+) currents in the PASMC of hyperoxic animals is normal, indicating that their inhibition is not sufficient to trigger HPV. Perinatal hyperoxia also abrogated responses elicited by hypoxia on catecholamine and cAMP metabolism in the CB. An increase in EPO plasma levels elicited by hypoxia was identical in hyperoxic and control animals, implying a normal functioning of EPO-producing cells. The loss of HPV observed in adult rats and caused by perinatal hyperoxia, comparable to oxygen therapy in premature infants, might represent a previously unrecognized complication of such a medical intervention capable of aggravating medical conditions such as regional pneumonias, atelectases or general anaesthesia in adult life.

Disclosing caffeine action on insulin sensitivity: effects on rat skeletal muscle.

Caffeine, a non-selective adenosine antagonist, has distinct effects on insulin sensitivity when applied acutely or chronically. Herein, we investigated the involvement of adenosine receptors on insulin resistance induced by single-dose caffeine administration. Additionally, the mechanism behind adenosine receptor-mediated caffeine effects in skeletal muscle was assessed. The effect of the administration of caffeine, 8-cycle-1,3-dipropylxanthine (DPCPX, A1 antagonist), 2-(2-Furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine (SCH58261, A2A antagonist) and 8-(4-{[(4-cyanophenyl)carbamoylmethyl]-oxy}phenyl)-1,3-di(n-propyl)xanthine (MRS1754, A2B antagonist) on whole-body insulin sensitivity was tested. Skeletal muscle Glut4,5'-AMP activated protein kinase (AMPK) and adenosine receptor protein expression were also assessed. The effect of A1 and A2B adenosine agonists on skeletal muscle glucose uptake was evaluated in vitro. Sodium nitroprussiate (SNP, 10nM), a nitric oxide (NO) donor, was used to evaluate the effect of NO on insulin resistance induced by adenosine antagonists. Acute caffeine decreased insulin sensitivity in a concentration dependent manner (Emax=55.54±5.37%, IC50=11.61nM), an effect that was mediated by A1 and A2B adenosine receptors. Additionally, acute caffeine administration significantly decreased Glut4, but not AMPK expression, in skeletal muscle. We found that A1, but not A2B agonists increased glucose uptake in skeletal muscle. SNP partially reversed DPCPX and MRS1754 induced-insulin resistance. Our results suggest that insulin resistance induced by acute caffeine administration is mediated by A1 and A2B adenosine receptors. Both Glut4 and NO seem to be downstream effectors involved in insulin resistance induced by acute caffeine.

Intermittent hypoxia and diet-induced obesity: effects on oxidative status, sympathetic tone, plasma glucose and insulin levels, and arterial pressure.

Obstructive sleep apnea (OSA) consists of sleep-related repetitive obstructions of upper airways that generate episodes of recurrent or intermittent hypoxia (IH). OSA commonly generates cardiovascular and metabolic pathologies defining the obstructive sleep apnea syndrome (OSAS). Literature usually links OSA-associated pathologies to IH episodes that would cause an oxidative status and a carotid body-mediated sympathetic hyperactivity. Because cardiovascular and metabolic pathologies in obese patients and those with OSAS are analogous, we used models (24-wk-old Wistar rats) of IH (applied from weeks 22 to 24) and diet-induced obesity (O; animals fed a high-fat diet from weeks 12 to 24) to define the effect of each individual maneuver and their combination on the oxidative status and sympathetic tone of animals, and to quantify cardiovascular and metabolic parameters and their deviation from normality. We found that IH and O cause an oxidative status (increased lipid peroxides and diminished activities of superoxide dismutases), an inflammatory status (augmented C-reactive protein and nuclear factor kappa-B activation), and sympathetic hyperactivity (augmented plasma and renal artery catecholamine levels and synthesis rate); combined treatments worsened those alterations. IH and O augmented liver lipid content and plasma cholesterol, triglycerides, leptin, glycemia, insulin levels, and HOMA index, and caused hypertension; most of these parameters were aggravated when IH and O were combined. IH diminished ventilatory response to hypoxia, and hypercapnia and O created a restrictive ventilatory pattern; a combination of treatments led to restrictive hypoventilation. Data demonstrate that IH and O cause comparable metabolic and cardiovascular pathologies via misregulation of the redox status and sympathetic hyperactivity.

Fernando de Castro and the discovery of the arterial chemoreceptors.

When de Castro entered the carotid body (CB) field, the organ was considered to be a small autonomic ganglion, a gland, a glomus or glomerulus, or a paraganglion. In his 1928 paper, de Castro concluded: "In sum, the Glomus caroticum is innervated by centripetal fibers, whose trophic centers are located in the sensory ganglia of the glossopharyngeal, and not by centrifugal [efferent] or secretomotor fibers as is the case for glands; these are precisely the facts which lead to suppose that the Glomus caroticum is a sensory organ." A few pages down, de Castro wrote: "The Glomus represents an organ with multiple receptors furnished with specialized receptor cells like those of other sensory organs [taste buds?]…As a plausible hypothesis we propose that the Glomus caroticum represents a sensory organ, at present the only one in its kind, dedicated to capture certain qualitative variations in the composition of blood, a function that, possibly by a reflex mechanism would have an effect on the functional activity of other organs… Therefore, the sensory fiber would not be directly stimulated by blood, but via the intermediation of the epithelial cells of the organ, which, as their structure suggests, possess a secretory function which would participate in the stimulation of the centripetal fibers." In our article we will recreate the experiments that allowed Fernando de Castro to reach this first conclusion. Also, we will scrutinize the natural endowments and the scientific knowledge that drove de Castro to make the triple hypotheses: the CB as chemoreceptor (variations in blood composition), as a secondary sensory receptor which functioning involves a chemical synapse, and as a center, origin of systemic reflexes. After a brief account of the systemic reflex effects resulting from the CB stimulation, we will complete our article with a general view of the cellular-molecular mechanisms currently thought to be involved in the functioning of this arterial chemoreceptor.

The effects of intermittent hypoxia on redox status, NF-κB activation, and plasma lipid levels are dependent on the lowest oxygen saturation.

Obstructive sleep apnea syndrome (OSAS) is described as repetitive obstructions of the upper airways during sleep, causing concomitant episodes of systemic hypoxia and associated cardiovascular and metabolic pathologies. The mechanisms generating these pathologies are controversial. Because recurrent hypoxia is the element of inadequate respiration that leads to the pathology, experimental models of OSAS consist in the exposure of the animals to intermittent hypoxia (IH) by cycling O2 percentages in their habitats. A proposed mechanism linking the IH of OSAS to pathologies is the increased production of reactive oxygen species (ROS). However, it has been argued that many patients seem to lack oxidative stress and that, to augment ROS in IH animals, intense hypoxia, seldom encountered in patients, has to be applied. To solve the controversy, we have exposed rats to two intensities of IH (cycles of 10 or 5% O2, 40s, and then 21% O2, 80s; 8h/day, 15 days). We then measured reduced and oxidized glutathione and lipid peroxide levels, aconitase and fumarase activities, and ROS-disposal enzyme activity in liver, brain, and lung. Liver levels of nuclear NF-κB-p65 and plasma C-reactive protein (CRP), as well as lipid levels, were also assessed. Lowest hemoglobin saturations were 91.7 ± 0.8 and 73.5 ± 1.4%. IH caused tissue-specific oxidative stress related to hypoxic intensity. Nuclear NF-κB-p65 and lipid content in the liver and CRP in the plasma all increased with IH intensity, as did both plasma triglycerides and cholesterol. We conclude that IH, even of moderate intensity, causes oxidative stress probably related to the pathologies encountered in OSAS patients.

Some reflections on intermittent hypoxia. Does it constitute the translational niche for carotid body chemoreceptor researchers?

The views presented in this article are the fruit of reflections and discussion with my colleagues at Valladolid and with the members of the Sleep Apnea Hypopnea Syndrome Group of the CIBERES (Spain). We have assembled the article in three sections. In the first one we provide a mechanistic description of obstructive sleep apnea (OSA) and all of its components, including the repetitive episodes of upper airways (UA) obstruction and accompanying hypoxic hypoxia, the respiratory efforts to fight and overcome the obstruction, and the sleep fragmentation due to the hypoxia-triggered arousal reactions, all events occurring during sleep hours with frequencies that might reach up >40-50 episodes/sleep hour. When OSA is accompanied by some of the elements of a big cohort of associated pathologies (vascular, metabolic, and neuropsychiatric) it conforms the obstructive sleep apnea syndrome (OSAS). The high frequency of OSAS in adults (>35 years old) and the costs in every regard of the treatment makes the syndrome a primary importance socio-sanitary problem. In the second section, we describe the experimental models of OSAS, basically the episodic repetitive hypoxic model described by Fletcher and coworkers in 1992, today named in short intermittent hypoxia (IH). From these lines, we want to call for some kind of consensus among researchers to lessen the dispersion of IH protocols. Finally, in the last section we intend to share our optimism with all ISAC members. The optimism is based on the recognition that carotid body (CB) chemoreceptors are critical elements of one of the main pathophysiologic loops in the genesis of OSAS. Therefore, we believe that all of us, as ISAC members, are well qualified to contribute in multidisciplinary research teams with well defined translational interests.

Dexamethasone down-regulates the inflammatory mediators but fails to reduce the tissue injury in the lung of acute pancreatitis rat models.

Pulmonary complications are frequent in the course of acute pancreatitis. We investigate the effects of dexamethasone on lung injury in mild and severe AP. Mild and severe acute pancreatitis was induced in rats by bile-pancreatic duct obstruction and infusion of 3.5% sodium taurocholate into the bile-pancreatic duct, respectively. Dexamethasone (1 mg/kg) was given by intramuscular injection 1 h after acute pancreatitis. Plasma amylase activity was measured to evaluate the pancreas damage. Lungs were harvested for analysing mRNA expression of monocyte chemoattractant protein-1 (MCP-1), cytokine-induced neutrophil chemoattractant (CINC), P-selectin and intercellular adhesion molecule-1 (ICAM-1), myeloperoxidase (MPO) activity (as index of neutrophil infiltration) and histological examination. Dexamethasone reduced the hyperamylasemia and hindered the pulmonary upregulation of MCP-1, CINC, P-selectin and ICAM-1, in both mild and severe acute pancreatitis. Despite this, dexamethasone treatment failed to reduce MPO activity and histological alterations developed in lungs during acute pancreatitis, either in bile-pancreatic duct obstruction or sodium taurocholate model. We conclude that pulmonary local factors different from inflammatory mediators contribute to leukocyte recruitment, so that although dexamethasone down-regulated the lung expression of chemokines and adhesion molecules during acute pancreatitis it was not able to prevent leukocyte infiltration, which could be responsible for maintaining the lung injury in either mild or severe acute pancreatitis.

Evaluation of N-acetylcysteine treatment in acute pancreatitis-induced lung injury.

OBJECTIVE: Pulmonary complications are frequent during acute pancreatitis (AP). We investigate the effects of N-acetylcysteine (NAC) on lung injury in mild and severe AP. ANIMALS AND TREATMENT: Mild and severe AP was induced in rats by bile-pancreatic duct obstruction (BPDO) and infusion of 3.5 % sodium taurocholate (NaTc) into the bile-pancreatic duct, respectively. NAC (50 mg/kg) was given 1 h before and 1 h after AP. METHODS: Amylase activity was measured in plasma. Lungs were harvested for mRNA expression analysis of monocyte chemoattractant protein-1 (MCP-1), cytokine-induced neutrophil chemoattractant (CINC), P-selectin and intercellular adhesion molecule-1 (ICAM-1), myeloperoxidase (MPO) activity and histological examination. RESULTS: Hyperamylasemia was reduced by NAC in both AP models. NAC down-regulated MCP-1, CINC and P-selectin in BPDO- but not in NaTc-induced AP. Pulmonary insults did not vary in mild AP and were exacerbated in severe AP by NAC treatment. NAC reduced lung MPO activity in mild but not in severe AP. CONCLUSIONS: Although NAC treatment down-regulated inflammatory mediators in lungs during AP it did not prevent leukocyte infiltration, which could be responsible for maintaining the lung injury. As a result, NAC aggravated the lung damage in severe AP and failed to exert beneficial effects in the mild disease model.

Effects of dexamethasone on intercellular adhesion molecule 1 expression and inflammatory response in necrotizing acute pancreatitis in rats.

OBJECTIVES: Adhesion molecules are involved in the inflammatory response during acute pancreatitis (AP). We investigated the effect of dexamethasone (Dx) on intercellular adhesion molecule 1 (ICAM-1) expression during AP and its consequences on leukocyte recruitment and pancreatic damage. METHODS: Acute pancreatitis was induced in rats by 3.5% sodium taurocholate for 3 hours and 6 hours. Dexamethasone (1 mg/kg) was administered either 30 minutes before or 1 hour after inducing AP. Messenger RNA ICAM-1 expression in pancreas and lung, membrane-bound ICAM-1 in acinar cells, and ICAM-1 plasma levels were analyzed. Histological examination of the pancreas and neutrophil infiltration in pancreas and lung were also measured. RESULTS: Prophylactic and therapeutic administration of Dx down-regulated ICAM-1 expression in pancreas and lung from early AP. Dexamethasone given before AP reduced the pancreatic damage, but lung inflammation was not prevented. Therapeutic Dx treatment was ineffective in avoiding leukocyte recruitment into the pancreas and lung in rats with AP. High ICAM-1 concentration was found in plasma during AP, which was not reduced by Dx treatments. CONCLUSIONS: Dexamethasone down-regulates ICAM-1 expression, but it does not completely prevent leukocyte recruitment during sodium taurocholate-induced AP.