Advances in Nanotechnology for Enhancing the Solubility and Bioavailability of Poorly Soluble Drugs.

This manuscript offers a comprehensive overview of nanotechnology's impact on the solubility and bioavailability of poorly soluble drugs, with a focus on BCS Class II and IV drugs. We explore various nanoscale drug delivery systems (NDDSs), including lipid-based, polymer-based, nanoemulsions, nanogels, and inorganic carriers. These systems offer improved drug efficacy, targeting, and reduced side effects. Emphasizing the crucial role of nanoparticle size and surface modifications, the review discusses the advancements in NDDSs for enhanced therapeutic outcomes. Challenges such as production cost and safety are acknowledged, yet the potential of NDDSs in transforming drug delivery methods is highlighted. This contribution underscores the importance of nanotechnology in pharmaceutical engineering, suggesting it as a significant advancement for medical applications and patient care.

Construction and practice of the integrated medical course of Molecular Medicine / 中华医学教育探索杂志

With the core of "molecules and cells", the integrated curriculum group of School of Basic Medical Sciences, Lanzhou University, focuses on the transfer of life molecules, reorganizes teaching content, and integrates Medical Cell Biology, Biochemistry and Molecular Biology, and Medical Genetics to construct a new integrated course of Molecular Medicine. The curriculum group actively explores and practices the mode of medical integration through reconstruction of the curriculum system and optimization of the course content. On the basis of establishing the online course system, the group explores the diversified teaching methods and evaluation systems suitable for Molecular Medicine and discusses the problems in curriculum construction.

Effect of high fat diet on stability of mitochondrial DNA/mRNA in the nerve cells of mice / 中华神经医学杂志

Objective To investigate the effects of high fat diet on stability of mitochondrial DNA/mRNA of frontal cortex tissue in mice.Methods Forty male mice were randomly divided into regular diet group and high fat diet group (n=20).All animals were sacrificed at 4 months (n=10) or 6 months (n=10) after experiment.The serum glucose,lipid and cholesterol levels were evaluated.And mitochondria were isolated in the frontal cortex of mice,and the changes ofmitochondrial DNA/mRNA were tested using PCR and real-time quantitative PCR.Results The serum glucose,lipid and cholesterol levels showed no significant changes in the mice of high fat diet group after feeding for 4 months as compared with those in the mice of regular diet group (P＞0.05),while the lipid level in the high fat diet group was increased significantly as compared with that in the regular diet group after feeding for 6 months (P＜0.05).The D-Loop level showed no significant changes after feeding for 4 and 6 months between the high fat diet group and the regular diet group (P＞0.05).Decreased ATPase6 and increasd Cyt3 and Cytb expression levels in the high fat diet group were noted as compared with those in the regular diet group after feeding for 6 months(P＜0.05).Conclusion The stability of mitochondrial DNA decreases in the brain of mice at the persistent feeding with high fat diet for 6 months; especially,pattem of mitochondrial mRNA expression changes obviously at early stage.

Effect of ovariectomy combined bondage stress on depression-like behavior and the hippocampal dentate gyrus progenitors in mice / 中华行为医学与脑科学杂志

Objective To explore whether synergistic action occurs in development of depression-like behaviors using ovariectomy combined bondage stress,and whether hippocampal neural progenitor state is changed following depression-like behaviors in mice.Methods Forty female C57B/L mice were divided into four groups randomly,which included ovariectomy,bondage stress,ovariectomy combined bondage stress and sham groups.Depression-like behaviors were evaluated by open field test,sucrose preference and forced swimming test after animals had been treated for 21 days.All animals were fixed by 4％ PA perfusion through left ventricle,then brain was removed,and coronal serials sections were made.Expression of Ki67 and DCX was tested by immunohistochemistry or immunofluorescence in subgranular zone of hippocampal dentate gyrus (SGZ).Results Body weight((18.70± 0.25) g vs (20.96±0.24)g),novelty-seeking behavior and sucrose preference decreased in ovariectomy combined bondage stress-group compared with those in sham group (P＜0.05),but forced swimming time increased in shame group((165.6±9.6)s vs (140.28±12.3) s).Likewisely,Ki67-positive cells((8.6±2.4)/section) and DCX-positive cells((4.2±1.4)/section) in SGZ decreased in ovariectomy combined bondage stress-group compared with those in sham group(Ki67:(16.7±2.5/section),DCX:(12.6±2.3/section) (P＜0.05).Unsimilarly,depressionlike behaviors had little change in ovariectomy-or bondage stress-groups compared with those in sham-group.Conclusion Ovariectomy combined bondage stress has synergistic action in development of depression,and inhibits the active state of progenitor in SGZ in mice.

Effect of Local Insulin Injection on Crushed Injury of Facial Nerve in Rats / 中国康复理论与实践

Objective To investigate the effect of local insulin injection on neurological function after crushed injury of facial nerve in rats. Methods Facial nerve was crushing injured in 30 Sprague-Dawley rats, from which 20 selected rats were divided into treatment group (n=10) and control group (n=10). The treatment group was injected with insulin 250 ng/kg at innervated muscle, and the control group was injected with normal saline. They were assessed with neurologic deficit score everyday within 7 days after injection, and the regeneration of nerve fiber was observed with retrograde fluorogold labeling. Results Compared with that in the control group, the neurological deficit score was improved more 5~7 days after injection in the treatment group, and the count of fluorogold labeling cells increased more in the treatment group. Conclusion Local injection of insulin can promote the recovery of neurological function after crushed injury of facial nerve.

Effect of Local Insulin Injection on Crushed Injury of Facial Nerve in Rats / 中国康复理论与实践

@#Objective To investigate the effect of local insulin injection on neurological function after crushed injury of facial nerve in rats. Methods Facial nerve was crushing injured in 30 Sprague-Dawley rats, from which 20 selected rats were divided into treatment group (n=10) and control group (n=10). The treatment group was injected with insulin 250 ng/kg at innervated muscle, and the control group was injected with normal saline. They were assessed with neurologic deficit score everyday within 7 days after injection, and the regeneration of nerve fiber was observed with retrograde fluorogold labeling. Results Compared with that in the control group, the neurological deficit score was improved more 5~7 days after injection in the treatment group, and the count of fluorogold labeling cells increased more in the treatment group. Conclusion Local injection of insulin can promote the recovery of neurological function after crushed injury of facial nerve.

Experimental Study of Effect of Aluminum on Learning and Memory and the Changes of Synaptic Interface Constructure in Hippocampus CA3 Area in Rats / 解剖科学进展

Objective　To study the effect of aluminum on learning and memory and the synaptic interface constructure in hippocampus CA3 area in rats. Methods　The electrical maze were used to test the behavioral reaction of rats. Some parameters of synaptic interface structure were measured by the method of ultrastructural quantitative analysis in hippocampus CA3 area in the rats. Results　The time coming through the E-maze prolonged siginificantly in the experimental group compared with the normal control (P＜0.01), meanwhile the cyto-apparatus demonstrated the pathogenetic changes. Amounts of synapse obviously reduced and the post-synaptic density turned thinner in experimental group than in the control group (P＜0.01). Conclusion　Aluminum can cause the malfunction on learning and memory, which related to the plastic of synapse.