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Bacterial infections are closely correlated with the genesis and progression of cancer, and the elimination of cancer-related bacteria may improve the efficacy of cancer treatment. However, the combinatorial therapy that utilizes two or more chemodrugs will increase potential adverse effects. Image-guided photodynamic therapy is a highly precise and potential therapy to treat tumor and microbial infections. Herein, four donor-acceptor-π-bridge-acceptor (D-A-π-A) featured near-infrared (NIR) aggregation-induced emission luminogens (AIEgens) (TQTPy, TPQTPy, TQTC, and TPQTC) with type I and type II reaction oxygen species (ROS) generation capabilities are synthesized. Notably, TQTPy shows mitochondria targeted capacity, the best ROS production efficiency, long-term tumor retention capacity, and more importantly, the three-in-one fluorescence imaging guided therapy against both tumor and microbial infections. Both in vitro and in vivo results validate that TQTPy performs well in practical biomedical application in terms of NIR-fluorescence imaging-guided photodynamic cancer diagnosis and treatment. Moreover, the amphiphilic and positively charged TQTPy is able to specific and ultrafast discrimination and elimination of Gram-positive (G+) Staphylococcus aureus from Gram-negative (G-) Escherichia coli and normal cells. This investigation provides an instructive way for the construction of three-in-one treatment for image-guided photodynamic cancer therapy and bacteria elimination.
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OBJECTIVE: The shallow underwater environment is complex, with problems of color shift, uneven illumination, blurring, and distortion in the imaging process. These scenes are very unfavorable for the reasoning of the detection network. Additionally, typical object identification algorithms struggle to maintain high resilience in underwater environments due to picture domain offset, making underwater object detection problematic. METHODS: This paper proposes a single-stage detection method with the double enhancement of anchor boxes and features. The feature context relevance is improved by proposing a composite-connected backbone network. The receptive field enhancement module is introduced to enhance the multi-scale detection capability. Finally, a prediction refinement strategy is proposed, which refines the anchor frame and features through two regressions, solves the problem of feature anchor frame misalignment, and improves the detection performance of the single-stage underwater algorithm. RESULTS: We achieved an effect of 80.2 mAP on the Labeled Fish in the Wild dataset, which saves some computational resources and time while still improving accuracy. On the original basis, UWNet can achieve 2.1 AP accuracy improvement due to the powerful feature extraction function and the critical role of multi-scale functional modules. At an input resolution of 300 × 300, UWNet can provide an accuracy of 32.4 AP. When choosing the number of prediction layers, the accuracy of the four and six prediction layer structures is compared. The experiments show that on the Labeled Fish in the Wild dataset, the six prediction layers are better than the four. CONCLUSION: The single-stage underwater detection model UWNet proposed in this research has a double anchor frame and feature optimization. By adding three functional modules, the underwater detection of the single-stage detector is enhanced to address the issue that it is simple to miss detection while detecting small underwater targets.
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Algoritmos , Peces , AnimalesRESUMEN
The application of face detection and recognition technology in security monitoring systems has made a huge contribution to public security. Face detection is an essential first step in many face analysis systems. In complex scenes, the accuracy of face detection would be limited because of the missing and false detection of small faces, due to image quality, face scale, light, and other factors. In this paper, a two-level face detection model called SR-YOLOv5 is proposed to address some problems of dense small faces in actual scenarios. The research first optimized the backbone and loss function of YOLOv5, which is aimed at achieving better performance in terms of mean average precision (mAP) and speed. Then, to improve face detection in blurred scenes or low-resolution situations, we integrated image superresolution technology on the detection head. In addition, some representative deep-learning algorithm based on face detection is discussed by grouping them into a few major categories, and the popular face detection benchmarks are enumerated in detail. Finally, the wider face dataset is used to train and test the SR-YOLOv5 model. Compared with multitask convolutional neural network (MTCNN), Contextual Multi-Scale Region-based CNN (CMS-RCNN), Finding Tiny Faces (HR), Single Shot Scale-invariant Face Detector (S3FD), and TinaFace algorithms, it is verified that the proposed model has higher detection precision, which is 0.7%, 0.6%, and 2.9% higher than the top one. SR-YOLOv5 can effectively use face information to accurately detect hard-to-detect face targets in complex scenes.
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Algoritmos , Reconocimiento Facial Automatizado/métodos , Cara/anatomía & histología , Redes Neurales de la Computación , Reconocimiento Facial Automatizado/estadística & datos numéricos , Biología Computacional , Aprendizaje Profundo , Humanos , Medidas de Seguridad/estadística & datos numéricosRESUMEN
A novel symmetric bianthracene derivative (D2) containing one benzene ring and two ethyl thioglycolates connecting to the benzene ring on both sides of the bianthracene unit was designed and synthesized. D2 can detect Fe3+ and Hg2+ in acetonitrile/water (6:4, v/v) solution via different changes of absorbance and fluorescence in the pH range from 3 to 10. D2 exhibits high colorimetric sensitivity for Fe3+ with low detection limit (1.87 × 10-5 mol/L). The absorbance intensity of D2 in acetonitrile/water solution show a linear response to Fe3+ in the wide concentration range of 0 -1000 µM, which is beneficial for quantitative analysis. D2 also displays highly selective fluorescent sensing for Hg2+ with a low detection limit of 1.07 µM over other metal ions and can accurately detect the existence of Hg2+ in water samples.
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Prior to the maturation of next-generation energy storage devices, the actual lithium-ion batteries for commercial purposes are still expected to fulfill some critical requirements, among which the high energy density, wide operating temperature range, and related long-term cycling stability are the most challenging issues. Herein a multiple additives strategy is employed to simultaneously optimize the solid electrolyte interphase on the large-area anode and cathode in a 2 Ah artificial graphite (AGr)/LiNi0.5Co0.2Mn0.3O2 (NCM523) pouch cell with high gravimetric (>260 Wh kg-1) and volumetric (>630 Wh L-1) energy density. By introducing a rational mixture of electrolyte additives, a highly sulfurized surface layer and a uniform and thin passivation layer are separately formed on the anode and cathode of the AGr/NCM523 pouch cell, exhibiting high storage stability at 60 °C, much improved discharge capacity at -10 and -20 °C, high anodic stability at high voltage of 4.4 V, and stable cyclic performance with a capacity retention of 85.5% after 500 cycles, significantly outperforming the value of 75.7% after only 200 cycles of the cell without additional additives. These results demonstrate the critical effect of simultaneous optimizations of anode and cathode interphase layers to construct stable high-energy-density lithium-ion pouch cells.
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A new indolo[2,3-a]carbazole based colorimetric chemosensor C1 was designed and synthesized. The optical properties of C1 were investigated by UV-vis spectroscopy, UV-vis titrations, and fluorescence spectroscopy. The HOMO and LUMO values of C1 were obtained using density functional theory (DFT) calculations. The results indicate that C1 has specific selectivity and high sensitivity for detection of Cu2+ and Fe3+ ions compared with metal ions (K+, Na+, Mg2+, Zn2+, Cd2+, Pb2+, Hg2+, Ag+, Co2+, Cr2+, Ni2+, Mn2+, Ba2+). The presence of Cu2+ ions leads to visible color change from yellow to colorless in an aqueous-acetonitrile solution, especially. Moreover, the detection limit for the analysis of Cu2+ and Fe3+ ions is found to be as low as 2.93 × 10-7 M and 4.10 × 10-7 M, respectively. Therefore, C1 should have potential applications as a practical colorimetric chemosensor for simultaneous detection of Cu2+ and Fe3+ in the environment and biological systems.
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Metallo-ß-lactamases (MBLs), such as New Delhi metallo-ß-lactamase (NDM-1) have spread world-wide and present a serious threat. Expression of MBLs confers resistance in Gram-negative bacteria to all classes of ß-lactam antibiotics, with the exception of monobactams, which are intrinsically stable to MBLs. However, existing first generation monobactam drugs like aztreonam have limited clinical utility against MBL-expressing strains because they are impacted by serine ß-lactamases (SBLs), which are often co-expressed in clinical isolates. Here, we optimized novel monobactams for stability against SBLs, which led to the identification of LYS228 (compound 31). LYS228 is potent in the presence of all classes of ß-lactamases and shows potent activity against carbapenem-resistant isolates of Enterobacteriaceae (CRE).
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Antibacterianos/farmacología , Proteínas Bacterianas/metabolismo , Enterobacteriaceae Resistentes a los Carbapenémicos/efectos de los fármacos , Monobactamas/farmacología , Resistencia betalactámica/efectos de los fármacos , beta-Lactamasas/metabolismo , Animales , Antibacterianos/efectos adversos , Antibacterianos/química , Antibacterianos/metabolismo , Aztreonam/farmacología , Células CHO , Cricetulus , Estabilidad de Medicamentos , Escherichia coli/efectos de los fármacos , Femenino , Humanos , Meropenem , Ratones , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Monobactamas/efectos adversos , Monobactamas/química , Monobactamas/metabolismo , Pseudomonas aeruginosa/efectos de los fármacos , Receptores de GABA-A/metabolismo , Convulsiones/inducido químicamente , Relación Estructura-Actividad , Tienamicinas/farmacologíaRESUMEN
Herein, a highly selective high-performance liquid chromatography (HPLC) coupled with resonance Rayleigh scattering (RRS) method was developed to detect gatifloxacin (GFLX) and sparfloxacin (SPLX). GFLX and SPLX were first separated by HPLC, then, in pH 4.4 Britton-Robinson (BR) buffer medium, protonic quaternary ammonia cation of GFLX and SPLX reacted with erythrosine (ERY) to form 1:1 ion-association complexes, which resulted in a significant enhancement of RRS signal. The experimental conditions of HPLC and post-column RRS have been investigated, including detection wavelength, flow rate, pH, reacting tube length and reaction temperature. Reaction mechanism were studied in detail by calculating the distribution fraction. The maximum RRS signals for GFLX and SPLX were recorded at λex = λem = 330 nm. The detection limits were 3.8 ng ml-1 for GFLX and 17.5 ng ml-1 for SPLX at a signal-to-noise ratio of 3. The developed method was successfully applied to the determination of GFLX and SPLX in water samples. Recoveries from spiked water samples were 97.56-98.85%.
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Antibacterianos/análisis , Cromatografía Líquida de Alta Presión/métodos , Eritrosina/química , Fluoroquinolonas/análisis , Cromatografía Líquida de Alta Presión/instrumentación , Gatifloxacina , Contaminantes Químicos del Agua/análisisRESUMEN
A novel, highly selective, and sensitive resonance light scattering (RLS) detection approach coupled with high performance liquid chromatography (HPLC) was researched and developed for the synchronous analysis of three kinds of benzimidazole anthelmintics, including mebendazole (MBZ), albendazole (ABZ), and fenbendazole (FBZ) for the first time. In the pH range of 3.5-3.7 Britton-Robinson buffer medium, three kinds of anthelmintics, which were separated by HPLC, reacted with eosin Y (EY) to form 1:1 ion-association complexes, resulting in significantly enhanced RLS signals and the maximum peak located at 335 nm. The enhanced RLS intensity was in proportion to the MBZ, ABZ, and FBZ concentration in the range 0.2-25, 0.2-23, and 0.15-20 µg/mL, respectively. The limit of detection was in the range of 0.064-0.16 µg/mL. In addition, human urine was determined to validate the proposed method by spiked samples and real urine samples. Satisfactory results were obtained by HPLC-RLS method. Graphical Abstract The diagram mechanism of generating resonance between emitted light and scattered light.
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Antihelmínticos/química , Bencimidazoles/química , Cromatografía Líquida de Alta Presión/métodos , Eosina Amarillenta-(YS)/química , Refractometría/métodos , Orina/química , Antihelmínticos/análisis , Bencimidazoles/análisis , Sitios de Unión , Colorantes Fluorescentes/química , Humanos , Concentración de Iones de Hidrógeno , Luz , Unión Proteica , Mapeo de Interacción de Proteínas , Reproducibilidad de los Resultados , Dispersión de Radiación , Sensibilidad y EspecificidadRESUMEN
Five novel metal-free organic dyes DQ1-5 containing a dipentyldithieno[3,2-f:2',3'-h]quinoxaline (DPQ) unit were synthesized and applied in dye-sensitized solar cells (DSSCs), where DPQ was employed as a π-spacer for the first time. Their photophysical, electrochemical, and theoretical calculations and photovoltaic properties were systematically investigated. All the five dyes show broad photoresponse. Especially the absorption edges of DQ3-5 extend to 800 nm on the TiO2 films. The inserted electron-rich unit 3,4-ethylenedioxythiophene or electron-withdrawing group benzothiadiazole (BTD) in DPQ-based dyes can greatly influence the optoelectronic properties of the dyes. In addition, the different electron donors also significantly affect the performance of the DSSCs. Under standard global AM 1.5 solar light conditions, the DQ5 sensitized solar cell obtained a power conversion efficiency of 7.12%. The result indicates that the rigid DPQ-based organic dye is a promising candidate for efficient DSSCs.
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Both animal communication sounds and human speech contain frequency-modulated (FM) sweeps. Although the selectivity for the rate of FM sweeps in neurons has been found in many kinds of animals at different levels of the central auditory structures, the underlying neural mechanism is still not clear. Using extracellular single unit recording techniques, we examined the selectivity for the rate of FM sweeps in the inferior colliculus (IC) neurons of the Kunming mouse (Mus musculus, Km) in the free-field stimulation conditions and determined its affecting factors. Totally, 102 neurons were recorded successfully, among which 42 neurons (41.2%) displayed a duration tuning pattern under pure tone (PT) stimulus. The percentages of short-pass, band-pass, and long-pass neurons were 22.6% (23/10), 8.8% (9/102), 9.8% (10/102), respectively. The other 60 neurons (58.8%) did not show any duration tuning features. Under FM stimulus, the majority of duration tuning neurons (78.6%, 33/42) showed the selectivity for the rate of FM sweeps. For these neurons, the type of rate selectivity was determined by the duration tuning features, but it was not related to the modulation range (MR) of FM. In a small fraction of duration tuning neurons (21.4%, 9/42), the rate selectivity was correlated with the MR, but uncorrelated with the duration tuning features. On the other hand, more than half of the non-duration tuning neurons (53.3%, 32/60) exhibited the rate selectivity under FM stimulus, and almost all of them (31/32) showed fast-rate selectivity. Nevertheless, there were 8 neurons (in 32) displaying the same best rate at different MR, indicating that they were real rate-selective neurons. Our results indicate that the selectivity for the rate of FM sweeps is co-determined by duration tuning features and sweep bandwidth. Only a few of inferior colliculus neurons belong to real rate-selectivity neurons in the mouse.
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Estimulación Acústica , Colículos Inferiores/citología , Neuronas/fisiología , Animales , RatonesRESUMEN
An on-line solid-phase extraction liquid chromatography/tandem mass spectrometry (SPE LC/MS/MS) assay using a newly developed SPE column and a monolithic column was developed and validated for direct analysis of plasma samples containing multiple analytes. This assay was developed in an effort to increase bioanalysis throughput and reduce the complexity of on-line SPE LC/MS/MS systems. A simple column-switching configuration that requires only one six-port valve and one HPLC pumping system was employed for on-line plasma sample preparation and subsequent gradient chromatographic separation. The resulting analytical method couples the desired sensitivity with ease of use. The method was found to perform satisfactorily for direct plasma analysis with respect to assay linearity, specificity, sensitivity, precision, accuracy, carryover, and short-term stability of an eight-analyte mixture in plasma. A gradient LC condition was applied to separate the eight analytes that cannot be distinctly differentiated by MS/MS. With a run time for every injection of 2.8 min, a minimum of 300 direct plasma injections were made on one on-line SPE column without noticeable changes in system performance. Due to the ruggedness and simplicity of this system, generic methods can be easily developed and applied to analyze a wide variety of compounds in a high-throughput manner without laborious off-line sample preparation.
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Cromatografía Liquida/métodos , Espectrometría de Masas/métodos , Preparaciones Farmacéuticas/sangre , Cromatografía Líquida de Alta Presión , Cromatografía Liquida/instrumentación , Espectrometría de Masas/instrumentación , Sensibilidad y EspecificidadRESUMEN
Through solution state NMR spectroscopy and quadrupole-time-of-flight mass spectrometry (Q-TOF MS) studies of the hexane insoluble botryal extract of the algae Botryococcus braunii race A, coupled with high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of the algaenan from this alga, it has been possible to advance the structural understanding of this geochemically important biopolymer. It was found that the hexane insoluble botryals in this study constitute a mixture of low molecular weight unsaturated aliphatic aldehydes and unsaturated hydrocarbons with an average chain length of about 40 carbons. Exact assignments were provided for many of the structural units present and describe the average constitution of the mixture as a whole. Reticulation of the low molecular weight chains via acetal and ester links explains the primary make up of algaenan. In this study, it is concluded that the algaenan results from the reticulation of low molecular weight hexane insoluble botryal species rather than the polyaldehyde as previously observed in studies of algae at alternate stages of their growth cycle.