Covalent organic frameworks (COFs) are featured with large specific surface areas, good thermal stability, and abundant pores. These properties are exactly what the sorbents used for extraction or adsorption of interest substances are desired with. While, the low density and hydrophobicity of COFs often makes them difficult to be dispersed evenly and recovered from the aqueous solution. Magnetic covalent organic frameworks (MCOFs) inherit magnetic property of the magnetic particles and porous structure of COFs. They have improved dispersity in aqueous solution and phase separation can be rapidly achieved via external magnetic fields. This review summarized the synthesis strategies for MCOFs, and their application in trace environmental organic pollutants analysis by chromatography techniques. The selection of COFs types and modification with active groups for a certain adsorption purpose is discussed, along with the exploration of adsorption mechanisms, which is beneficial for the design and synthesis of MCOFs.
We demonstrate a multichannel entropy loading mechanism in an optical frequency comb-based coherent communication system. In high-capacity wavelength division multiplexing communications, the individual laser sources can be replaced by an optical frequency comb, thus reducing the complexity and energy consumption of the transmitter. However, the power variation among different comb lines will lead to performance discrepancies. Spectral flattening filters can be adopted to suppress the variation at the expense of an additional system loss. Alternatively, by applying probabilistic shaping, we have implemented multichannel entropy loading to facilitate a continuous adaptation of the source entropy. The data rate can be dynamically allocated according to the performance of each channel. Through the loading scheme, the non-uniform performances across the channels are effectively mitigated, achieving a capacity enhancement of 34.91â Gbit/s.
Patients on maintenance haemodialysis (MHD) face social isolation due to the far-reaching effects of their disease and treatment. Based on the Transactional Model of Stress and Coping, we analysed the relationship between perceived stress and social isolation in patients, and the mediating role of internal health-related locus of control (IHLC) from the perspective of co-existence of perceived helplessness (HEL) and perceived self-efficacy (SEL). We used structured questionnaires to investigate 332 cases of young and middle-aged MHD patients in four tertiary hospitals in Guangdong, China. Polynomial regression and response surface analysis were conducted, along with tests of mediating effects on the data. The research was based on STROBE guidelines. The results showed that perceived stress is significantly and positively associated with social isolation. When the HEL and SEL of perceived stress were congruent, the joint effect of the two was in a positive curvilinear relationship to social isolation, when the two were incongruent, the risk of social isolation was lower for "low HEL-high SEL" patients compared to the "high HEL-low SEL" combination. The more congruent in HEL -SEL, the lower the risk of social isolation for the patients, with the IHLC playing a mediating role in this association. This study reveals that the coexistence of perceived stress's HEL and SEL affects social isolation through congruent and incongruent matching mechanism, providing additional explanations for the mechanism of occurrence of patients' social isolation.
Emotions , Social Isolation , Middle Aged , Humans , Surveys and Questionnaires , Stress, Psychological , Renal Dialysis
Abundant biomass resources provide us with sufficient material basis, while a large amount of bio-waste is also produced and the high-value utilization of bio-waste is still highly desirable. Herein, we reported a facile one-pot fabrication approach towards efficient utilization of sugarcane bagasse via carboxymethylation to adsorb and recycle Cu2+ ions. The modified sugarcane bagasse possessed outstanding adsorption efficiency, with a maximum capacity of 263.7 mg g-1, owing to the functional groups such as carboxyl and hydroxyl groups, as well as aromatic structure. It was noted that the carboxymethylated sugarcane bagasse (MSB40) swelled rapidly when suffering Cu2+ ions solution, and more adsorption sites were available since the physical diffusion barrier was removed, thereby enhancing the absorption capacity. Interestingly, Cu2+ ions could induce the aggregation of MSB40 due to the Cu2+ ions compress colloid double layer, neutralizes surface charges, which benefited the following separation process. Ultimately, copper oxide was recovered and the purity reached 97.9%. Additionally, in the presence of both Ca2+ and Mg2+ ions, MSB40 exhibited excellent selectivity for the adsorption of Cu2+ ions. This strategy offers a facile and novel clue for the high-value utilization of bio-waste and the recovery of copper for biomaterial and environmental applications.
We propose a probability mass function (PMF) optimization scheme for quadrature amplitude modulation (QAM) signals by considering the parametric characteristic of the training sequence. The training sequence for optimization is mapped in standard Maxwell-Boltzmann (M-B) distribution, and the considered characterizing parameters incorporate either the noise variance or the error matrix of the received symbols. The proposed PMF optimization is based on independent reallocation within each constellation ring, generating new distribution with almost the same entropy and transmitted power as the original distribution. This reallocation mechanism is model-free and iterative-free with very low computational complexity. By characterizing the channel in terms of constellation performance asymmetry, PMF reallocation can be effectively implemented to supplement the existing equalization algorithm. The effectiveness of this approach is experimentally verified in a 40-km transmission system with 24 Gbaud 64-QAM signals under three different scenarios. Through PMF reallocation, we achieve generalized mutual information (GMI) improvement of â¼0.06 and throughput improvement of â¼1.5 Gbit/s before forward error correction in comparison with the standard M-B distribution. The proposed mechanism provides a solution to obtain the optimal PMF in practical communication channels, which suffer from various types of noises and distortions.
Electroacupuncture (EA) is an efficient treatment for visceral hypersensitivity (VH). However, the mechanism underlying VH remains obscure. This study aimed to examine the effect of EA at Housanli acupoint on PAR2 and PAR4 expression in the periaqueductal gray (PAG), rostral ventromedial medulla (RVM), and spinal cord dorsal horn (SCDH) axes, as well as on expression of the proinflammatory cytokines IL-1ß and TNF-α, COX-2 enzyme, c-Fos, and the neuropeptides CGRP and SP in the same areas of the descending pain modulatory system. To induce VH in male goats, a 2,4,6-trinitrobenzene-sulfonic acid (TNBS)-ethanol solution was administered to the ileal wall. The visceromotor response (VMR) and nociceptive response at different colorectal distension pressures were measured to evaluate VH. Goats in the TNBS group displayed significantly increased VMR and nociceptive response scores, and elevated protein and mRNA levels of PAR2 and PAR4 in the descending pain modulatory system compared to those in the control group. EA alleviated VMR and nociceptive responses, decreased the protein and mRNA expression levels of PAR2, and elevated those of PAR4 in the descending pain modulatory system. EA may relieve VH by reducing PAR2 expression and increasing PAR4 expression in the descending pain modulatory system.
A broadband photonic-assisted microwave receiver with high cross-channel interference suppression and image rejection is proposed and experimentally demonstrated. At the input of the microwave receiver, a microwave signal is injected into an optoelectronic oscillator (OEO), which functions as a local oscillator (LO) to generate a low-phase noise LO signal as well as a photonic-assisted mixer to down-convert the input microwave signal to the intermediate frequency (IF). A microwave photonic filter (MPF), realized by the joint operation of a phase modulator (PM) in the OEO and a Fabry-Perot laser diode (FPLD), is used as a narrowband filter to select the IF signal. Thanks to the wide bandwidth of the photonic-assisted mixer and the wide frequency tunable range of the OEO, the microwave receiver can support broadband operation. The high cross-channel interference suppression and image rejection are enabled by the narrowband MPF. The system is evaluated experimentally. A broadband operation from 11.27 to 20.85â GHz is demonstrated. For a multi-channel microwave signal with a channel spacing of 2â GHz, a cross-channel interference suppression ratio of 21.95â dB and an image rejection ratio of 21.51â dB are realized. The spurious-free dynamic range (SFDR) of the receiver is also measured to be 98.25â dB·Hz2/3. The performance of the microwave receiver for multi-channel communications is also experimentally evaluated.
Two-dimensional ferromagnetic Weyl half-metals that are robust against spin-orbital coupling were theoretically proposed recently, in which the nodal points and the nodal loops are protected by specific symmetries. As the symmetry of a ferromagnetic material is highly dependent on the magnetization orientation, here we predict a family of two-dimensional ferromagnetic Weyl half-metals, Mn2X3 (X = S, Se, Te) monolayers, to investigate the band topology under different magnetization orientations in the presence of spin-orbital coupling. The Curie temperatures (â¼1000 K) were estimated to be much higher than room temperature due to the strong double exchange interaction and the suppression of spin fluctuation for the two-sublayer structure. Taking a Mn2Te3 monolayer as an example, we demonstrated the evolution of the nodal points and the nodal loops in the presence of spin-orbital coupling via manipulating magnetization orientation. Our work provides a family of high temperature two-dimensional ferromagnetic Weyl half-metals for investigating the nontrivial band topology.
A porous aromatic framework (PAF-47) synthesized through Suzuki coupling reaction was introduced to prepare PAF-47/polydimethylsiloxane (PDMS) coated stir bar by sol-gel technique. PAF-47/PDMS coating provided high extraction recovery (77.6-90.6%, the ratio of actual enrichment factor (EF) to theoretical EF) for five polychlorinated biphenyls (PCBs) in a relatively short time (60 min), exhibiting a faster extraction kinetics over commercial PDMS coating (12/24 h). Based on this, a new method based on PAF-47/PDMS coated stir bar sorptive extraction and high-performance liquid chromatography-diode array detection was proposed for trace analysis of target PCBs in environmental water. Under the optimized conditions, the limits of detection for five PCBs were within 44-70 ng/L, with actual EF of 64.0-71.5-fold (maximal EF of 83.3-fold). This method was successfully used to detect trace PCBs in Yangtze River water and East Lake water, with recoveries of 81.0-113% and 86.1-111%, respectively.
Polychlorinated Biphenyls , Water Pollutants, Chemical , Polychlorinated Biphenyls/analysis , Chromatography, High Pressure Liquid/methods , Porosity , Limit of Detection , Water , Dimethylpolysiloxanes/analysis , Reproducibility of Results , Water Pollutants, Chemical/analysis
Hepatic steatosis is a highly prevalent liver disease, yet research on it is hampered by the lack of tractable cellular models in poultry. To examine the possibility of using organoids to model steatosis and detect it efficiently in leghorn male hepatocellular (LMH) cells, we first established steatosis using different concentrations of oleic acid (OA) (0.05-0.75 mmol/L) for 12 or 24 h. The subsequent detections found that the treatment of LMH cells with OA resulted in a dramatic increase in intracellular triglyceride (TG) concentrations, which was positively associated with the concentration of the inducing OA (R2 > 0.9). Then, the modeled steatosis was detected by flow cytometry after NileRed staining and it was found that the intensity of NileRed-A was positively correlated with the TG concentration (R2 > 0.93), which demonstrates that the flow cytometry is suitable for the detection of steatosis in LMH cells. According to the detection results of the different steatosis models, we confirmed that the optimal induction condition for the establishment of the steatosis model in LMH cells is OA (0.375 mmol/L) incubation for 12 h. Finally, the transcription and protein content of fat metabolism-related genes in steatosis model cells were detected. It was found that OA-induced steatosis could significantly decrease the expression of nuclear receptor PPAR-γ and the synthesis of fatty acids (SREBP-1C, ACC1, FASN), increasing the oxidative decomposition of triglycerides (CPT1A) and the assembly of low-density lipoproteins (MTTP, ApoB). Sterol metabolism in model cells was also significantly enhanced (HMGR, ABCA1, L-BABP). This study established, detected, and analyzed an OA-induced steatosis model in LMH cells, which provides a stable model and detection method for the study of poultry steatosis-related diseases.
Fatty Liver , Oleic Acid , Male , Animals , Oleic Acid/metabolism , Lipid Metabolism , Chickens/metabolism , Fatty Liver/chemically induced , Fatty Liver/veterinary , Fatty Liver/metabolism , Fatty Acids/metabolism , Liver/metabolism
Metformin, the first-line oral antidiabetic medicine, has shown great antineoplastic potential in various cancer types, despite an unclear mechanism. This study aimed to elucidate the possible mechanism of metformin as a chemotherapy agent with less reproductive and genetic toxicity in human endometrial cancer. The type I endometrial carcinoma cell lines Ishikawa and RL95-2 were treated with metformin. Cell functions, such as proliferation, migration, and invasion, were analyzed. Flow cytometry was performed for cell cycle and apoptosis analyses. Simultaneously, RT-qPCR and western blotting were performed to explore the possible mechanism. Moreover, YAP1 knockout Ishikawa cells were established via lentivirus to demonstrate the underlying mechanism. The results showed that metformin mediated Ishikawa and RL95-2 cell growth inhibition in a dose- and time-dependent manner. The IC50 values of metformin in Ishikawa and RL95-2 cells were 10 mM and 8 mM, respectively. The migration and invasion abilities were also inhibited in the metformin-treated group using wound healing assays and transwell migration and invasion assays, and Ishikawa and RL95-2 cells were arrested in the G1 or G2 phase, respectively. Moreover, the cell proportions of cells in both early and late apoptosis stages were dramatically elevated when treated with metformin, as was the ratio of Bax/Bcl-2 expression. Additionally, the expression levels of YAP1 mRNA and protein in the treatment group were much lower than those in the control group. The cellular behaviors of YAP1 knockout Ishikawa cells were similar to those in the metformin-treated group. Our results demonstrated that it is an attractive alternative to cytotoxic chemotherapy in human endometrial cancer, and YAP of the Hippo pathway may be a potential molecular target. This study provides novel ideas for the adjuvant therapy of endometrial cancer patients, especially for women with strong fertility desires and demands.
In this work, porous aromatic frameworks (PAFs) with different pore size were evaluated for simultaneous adsorption of 16 polycyclic aromatic hydrocarbons (PAHs) with large difference in polarity and molecular size. Two other porous organic polymers containing electron pushing/withdrawing group were investigated along for a comparison, and PAF-120 with the pore size of appr. 2.1 nm exhibited the highest extraction efficiency. Based on water contact angle and molecular dynamics simulation, the adsorption of 16 PAHs on PAF-120 was attributed to hydrophobic interaction, π-π interaction and molecular sieving effect. PAF-120/PDMS coated stir bar was then prepared by physical adhesion, and a method of stir bar sorptive extraction-gas chromatography-flame ionization detector was established for trace PAHs analysis in environmental samples. Under the optimal experimental conditions, the limits of detection (S/N = 3) for 16 PAHs were found to be in the range of 42-375 ng/L, with the relative standard deviations of 4.1-14.6% (n = 7). The enrichment factors varied from 31 (Indeno[1,2,3-cd]pyrene) to 80-fold (anthracene), with the maximal enrichment factor of 100-fold. The proposed method was applied to the analysis of PAHs in local environmental water and atmospheric particle samples. None of the 16 PAHs were detected in the collected water samples. While for the collected atmospheric particles, 12 PAHs were detected in fine particulate matter (PM2.5) within the range of 0.6-2.8 ng/m3. For inhalable particulate matter (PM10) and total suspended particulate matter (TSP), 16 PAHs were all detected in the range of 0.6-3.8 ng/m3 and 0.6-5.9 ng/m3, respectively. Quantitative recoveries were obtained in recovery test, demonstrating the accuracy and application potential of the proposed method.
Polycyclic Aromatic Hydrocarbons , Water Pollutants, Chemical , Chromatography, Gas/methods , Limit of Detection , Particulate Matter/analysis , Polycyclic Aromatic Hydrocarbons/analysis , Porosity , Water , Water Pollutants, Chemical/analysis
α2-Adrenegic receptors (α2Rs) are important presynaptic modulators of central noradrenergic function (auto receptors) and postsynaptic mediators of many of the widespread effects of catecholamines and related drugs. Studies have shown that ruminants (such as goats and cattle) express special α2DR subtypes in addition to α2BR and α2CR. Real-time quantitative PCR and Western blotting were used to investigate the distribution and density of α2R in different nuclei of the goat central nervous system, selected regions of the spinal cord (L4-L6), and in various peripheral tissues. α2-AR subtype-specific antibodies were injected intrathecally and intracerebroventricularly into the tested goats to block the corresponding subtype of receptors. Pain threshold and physiological parameters were evaluated to explore the functional characteristics of α2BR, α2CR and α2DR in goats. Our results suggest that the expression of the mRNAs and proteins of all three α2R subtypes are widely but unevenly distributed in the goat CNS and peripheral tissues. Furthermore, α2DR plays a more important role in α2R-mediated analgesia in goats than α2BR and α2CR, whereas α2CR activation exerts a greater effect on body temperature than α2BR and α2DR.
Goats have been used as animal models in research, and the need for achieving safer anesthesia for research or surgical intervention is gaining much attention. The objective of this study was to evaluate intraoperative effects and the immediate postoperative analgesia of nalbuphine-ketamine regimen in goats. Twenty clinically healthy adult female crossbred goats weighing 14 ± 2 kg were allocated randomly into each of two equally sized groups. All animals were sedated with intramuscular (IM) xylazine (0.07 mg/kg), then anesthesia was intravenously (IV) induced with ketamine alone (10 mg/kg) (XK group), or a combination of nalbuphine (0.5 mg/kg) and ketamine (5 mg/kg) (XNK group). Following induction, left flank laparotomy was performed and then sutured. The quality of anesthesia and immediate postoperative analgesia was evaluated. Immediate postoperative analgesia was assessed up to 5 h after standing, using a modified Unesp-Botucatu acute composite pain scale (USAPS). Serum cortisol, glucose, insulin, and C-reactive protein (CRP) were measured at ½, 1, 2, 4, 6, 12, and 24 h, postoperatively (PO). The USAPS pain scores were significantly lower in the XNK compared to the XK group (p < 0.05). The XNK group exhibited a statistically significant difference in the level of serum cortisol at ½ and 1 h PO (p = 0.018 and 0.045, respectively) compared to the XK group. At 2, 4, 6 h PO, CRP significantly decreased (p = 0.023, 0.040 and 0.005, respectively) in the XNK compared to the XK group. Nalbuphine-ketamine produced an acceptable induction of anesthesia and recovery compared to ketamine. Recovery with nalbuphine-ketamine was faster and better quality. The USAPS pain scores were lower in nalbuphine-ketamine, indicating that this novel combination produces better postoperative pain control than ketamine alone.
A series of LaCoO3 perovskite catalysts substituted by Sr in the A site (La1-xSrxCoO3) were prepared via a facile sol-gel method. The catalytic activity of these perovskite catalysts for the deep oxidation of toluene was evaluated. It was found that Sr substitution significantly enhanced the redox properties, the concentration of oxygen vacancies, and surface Co3+ active species via an electron interaction between Sr and Co from the results of Raman spectroscopy, H2-TPR (temperature programmed reduction), O2-TPD (temperature programmed desorption) and XPS (X-ray photoelectron spectroscopy). Typically, La0.82Sr0.18CoO3 (L0.82S0.18C) exhibited a superior catalytic performance among these samples owing to its best reducibility and highest number of active species. Kinetic analysis further revealed a higher reaction rate (5.1 × 10-7 mol g-1·s-1 at 210 °C) and a lower apparent activation energy (69.1 kJ mol-1) for toluene oxidation on the L0.82S0.18C sample in comparison to those on the others. In situ DRIFTS (diffuse reflectance infrared Fourier transform spectroscopy) confirmed the easy desorption of immediate products from the surface of the L0.82S0.18C sample, which could be responsible for its remarkable performance. These results could provide an efficient strategy for promoting the toluene oxidation through finely tuning the reducibility and surface active phase of the catalysts.
The Fe-Ti-Ox catalysts with the different Fe contents were used for the catalytic hydrolysis of hydrogen cyanide (HCN) in the presence of H2O, which investigated the roles of Fe chemical valence and oxygen species in HCN removal and the production (NH3 and CO). The results implied that more amounts of Fe3+ species over Fe-Ti-Ox could increase the catalytic hydrolysis activity of HCN while Fe2+ species contributed to the formation of NH3 at high temperatures. Furthermore, the abundance of surface oxygen species was in favour of the catalytic performance of HCN.
Hydrogen Cyanide , Titanium , Ammonia/chemistry , Oxidation-Reduction , Oxygen , Titanium/chemistry
The use of porous carrier and coating sorbents in stir bar sorptive extraction (SBSE) contributes to the improvement of extraction efficiency and dynamics. Herein, porous nickel foam (NF) with large surface area and magnetic property was used as the carrier of stir bar. NF was added into the mixture of graphene oxide (GO) and reducing agent, and reduced graphene oxide (RGO) coating was obtained on the surface of NF substrate by in situ hydrothermal reduction. The characterization results of Fourier transform infrared spectroscopy, X-ray power-diffraction and scanning electron microscope showed that GO was partially reduced into RGO, and the RGO coating was uniformly loaded on the NF surface. The obtained RGO-NF composite was used as the stir bar coating for the analysis of six benzotriazole (BZTs) UV absorbents. The extraction efficiency was between 48 and 64% for six BZTs. RGO-NF stir bar exhibited faster adsorption/desorption kinetics than commercial polydimethylsiloxane coated stir bar (50 min vs 120/360 min) due to its porous structure and large specific surface area. On this basis, a method of RGO-NF coated stir bar sorptive extraction combined with high performance liquid chromatography (HPLC)-DAD was established for the determination of six BZTs. Under the optimized conditions, the limits of detection were 0.33-0.50 µg/L for six target BZTs, and the linear range was 1-100 µg/L. The proposed method merits good ability to resist matrix and was used to analyze six BZTs in environmental water samples. The recoveries of target BZTs were obtained within 83.0-112% in the spiked East Lake water and 97.0-111% in the spiked Yangtze River water, respectively.
We demonstrate carrier-to-signal power ratio (CSPR) enhancement by self-seeded stimulated Brillouin scattering to improve the performance of Kramers-Kronig (KK) detection for multichannel single-sideband (SSB) signals. By virtue of low-CSPR transmission and high-CSPR detection, our proposed scheme effectively advances system performance by reducing propagation-induced distortion while maintaining the minimum phase condition. We experimentally demonstrate the improvement in CSPR and bit error rate of 5×10-Gbaud 16-QAM SSB signals by applying the carrier recovery block after 80-km transmission. Under optimum pump power, the average Q factor improvement of all five channels is 3.0 dB. We also analyze the performances of different channels and the major limiting factor. The results verify that our scheme offers a promising solution to enhance SSB self-coherent KK detection in wavelength-division multiplexing systems.
Recent progress of stir bar sorptive extraction (SBSE) in the past six years is reviewed. The preparation methods including electrodeposition, self-assembly, solvent exchange, physical magnetic adsorption and electrostatic spinning, for the coated stir bar are summarized and compared, specifically for a specific material for coatings fabrication, e.g., carbon-based materials and metal organic frameworks. The emerging materials (e.g., graphene, graphene oxide, carbon nanotubes, monolith, metal-organic frameworks and porous organic polymers) applied for coated stir bar fabrication are one of the focus of this review, along with their respective advantages in extraction process and application in trace analysis. The development and application of extraction apparatus of SBSE are also involved. Based on these information, the development status and prospects of SBSE as an efficient sample pretreatment technique in real sample analysis are discussed.
Nanotubes, Carbon/chemistry , Adsorption , Metal-Organic Frameworks/chemistry , Polymers , Porosity , Reproducibility of Results , Solvents
Accumulated evidence has shown that the photosensitizer Verteporfin (VP) may be an ideal agent for various cancer types. However, the effect and mechanism of VP on human cervical carcinoma remain rudimentary. The aim of this study was to investigate the effect of VP on human cervical carcinoma cells (HeLa and SiHa cells) and to elucidate the possible mechanism. CCK-8, wound healing assay, flow cytometry analysis, western blotting, TUNEL staining were performed to evaluate the effects of VP on HeLa and SiHa cells in vitro as well as in vivo on a xenograft model. In addition, the role of endoplasmic reticulum (ER) stress in VP-induced apoptosis was investigated using RT-qPCR and western blotting. The results showed that the viability of HeLa and SiHa cells was suppressed by VP in dose- and time-dependent manners. Compared with the control group, apoptosis rates were higher with stronger TUNEL fluorescence signals in the experimental group, which substantiated that VP induced apoptosis at both 2D and 3D cell levels. Besides, VP can squelch the growth of tumors in both sizes and weights on the xenograft models without impairing ovarian reserve. Mechanism studies demonstrated that VP activated ER stress by upregulating the expression of GRP78, CHOP, and Caspase-12, and VP-induced apoptosis can be alleviated when ER stress pathway was inhibited. Our results provided a foundation for repurposing VP as a promising agent for cervical cancer patients without obvious reproductive toxicity by targeting ER stress pathway, and more researches are required to support its application in clinical practice.
