Purpose: The aim of this study is to investigate the effects of a preoperative combined with postoperative moderate-intensity progressive resistance training (PRT) of the operative side in patients with hip osteoarthritis (HOA) who are undergoing total hip arthroplasty (THA). The study seeks to evaluate the impact of this combined intervention on muscle strength, gait, balance, and hip joint function in a controlled, measurable, and objective manner. Additionally, the study aims to compare the outcomes of this combined intervention with those of preoperative or postoperative muscle strength training conducted in isolation. Methods: A total of 90 patients with HOA scheduled for unilateral primary THA were randomly assigned to three groups: Pre group (preoperative PRT), Post group (postoperative PRT), and Pre& Post group (preoperative combined with postoperative PRT) focusing on hip flexion, extension, adduction, and abduction of operated side. Muscle strength, gait parameters, balance, and hip function were assessed at specific time points during a 12-month follow-up period. Results: All three groups showed significant improvements in muscle strength, with the Pre& Post group demonstrating the most pronounced and sustained gains. Gait velocity and cadence were significantly improved in the Pre& Post group at 1-month and 3-month postoperative follow-ups compared to the other groups. Similarly, the Pre& Post group exhibited superior balance performance at 3-month and 12-month postoperative follow-ups. The Harris Hip Score also showed better outcomes in the Pre& Post group at all follow-up intervals. Conclusion: Preoperative combined with postoperative moderate-intensity PRT in HOA patients undergoing THA led to superior improvements in muscle strength, gait, balance, and hip joint function compared to preoperative or postoperative PRT alone. This intervention shows significant promise in optimizing postoperative rehabilitation and enhancing patient outcomes following THA.
Arthroplasty, Replacement, Hip , Gait , Muscle Strength , Osteoarthritis, Hip , Postural Balance , Resistance Training , Humans , Arthroplasty, Replacement, Hip/rehabilitation , Male , Female , Resistance Training/methods , Aged , Middle Aged , Osteoarthritis, Hip/surgery , Prospective Studies , Range of Motion, Articular , Treatment Outcome , Hip Joint/surgery , Postoperative Period
P2-type Na0.67Ni0.33Mn0.67O2 has attracted considerable attraction as a cathode material for sodium-ion batteries owing to its high operating voltage and theoretical specific capacity. However, when the charging voltage is higher than 4.2 V, the Na0.67Ni0.33Mn0.67O2 cathode undergoes a detrimental irreversible phase transition of P2-O2, leading to a drastic decrease in specific capacity. To address this challenge, we implemented a Cu-doping strategy (Na0.67Ni0.23Cu0.1Mn0.67O2) in this work to stabilize the structure of the transition metal layer. The stabilization strategy involved reinforcing the transition metal-oxygen (TMO) bonds, particularly the MnO bond and inhibiting interlayer slip during deep desodiation. As a result, the irreversible phase transition voltage is delayed, with the threshold voltage increasing from 4.2 to 4.4 V. Ex-situ X-ray diffraction measurements revealed that the Na0.67Ni0.23Cu0.1Mn0.67O2 cathode maintains the P2 phase within the voltage window of 2.5-4.3 V, whereas the P2-Na0.67Ni0.33Mn0.67O2 cathode transforms entirely into O2-type Na0.67Ni0.33Mn0.67O2 when the voltage exceeds 4.3 V. Furthermore, absolute P2-O2 phase transition of the Na0.67Ni0.23Cu0.1Mn0.67O2 cathode occurred at 4.6 V, indicating that Cu2+ doping enhances the stability of the layer structure and increases the threshold voltage. The resulting Na0.67Ni0.23Cu0.1Mn0.67O2 cathode exhibited superior electrochemical properties, demonstrating an initial reversible specific capacity of 89.1 mAh/g at a rate of 2C (360 mA g-1) and retaining more than 78 % of its capacity after 500 cycles.
Transition metal selenides are a research hotspot in sodium-ion batteries (SIBs). However, slow kinetics and rapid capacity decay due to volume changes during cycling limit their commercial applications. Heterostructures have the ability to accelerate charge transport and are widely used in energy storage devices due to their abundant active sites and lattice interfaces. A rational design of heterojunction electrode materials with excellent electrochemical performance is essential for SIBs. Herein, a novel anode material heterostructured FeSe2/MoSe2 (FMSe) nanoflower for SIBs was successfully prepared through a facile co-precipitation and hydrothermal route. The as-prepared FMSe heterojunction exhibits excellent electrochemical performance, including a high invertible capacity (493.7 mA h g-1 after 150 cycles at 0.2 A g-1), long-term cycling stability (352.2 mA h g-1 even after 4200 cycles at 5.0 A g-1) and competitive rate capability (361.2 mA h g-1 at 20 A g-1). By matching with a Na3V2(PO4)3 cathode, it can even exhibit ideal cycling stability (123.5 mA h g-1 at 0.5 A g-1 after 200 cycles). Furthermore, the sodium storage mechanism of the FMSe electrodes was systematically determined by ex situ electrochemical techniques. Theoretical calculation also reveals that the heterostructure on the FMSe interface enhances charge transport and promotes reaction kinetics.
The phosphate interphase of anisotropic amorphous vanadium oxide (VO-PO@CNPs) can prevent structural reorganization and enrich ion diffusion paths in cathode materials for aqueous zinc ion batteries. VO-PO@CNPs cathodes achieve a high initial capacity, the average capacity reduction rate is just 0.13% for each cycle, and durability is prolonged to 280 hours at 0.2 A g-1.
Lithium (Li) metal is an excellent anode of Li ion batteries because of its high theoretical capacity and the low redox potential compared to other anodes. However, the uncontrollable growth of Li dendrites still incurs serious safety issues and poor electrochemical performances, leading to its limited practical application. An oxygen and boron codoped honeycomb carbon skeleton (OBHcCs) is reported and a stable Li metal-based anode is realized. It can be coated on a copper foil substrate to be used as a current collector for a dendrite-free Li metal anode. OBHcCs effectively reduces the local current density owing to the high surface area and inhibits Li dendrite growth, which is explored by scanning electron microscopy and an X-ray photoelectron spectra depth profile. The abundant lithiophilic oxygen and boron-containing functional groups reduce the potential barrier of nucleation and lead to the homogeneous Li ions flux as confirmed by the density functional theories. Therefore, the Li metal anode based on OBHcCs (OBHcCs@Li) stably runs for 700 h in a symmetric cell with a Li stripping capacity of 1 mAh cm-2 at 1 mA cm-2 . Furthermore, the OBHcCs@Li|LiFePO4 full cell shows a good capacity retention of 84.6% with a high coulombic efficiency of 99.6% at 0.5 C for 500 cycles.
As for hybrid supercapacitors, it is important to enhance the long cycling performance and high specific capacitance. In this paper, cobalt vanadate (Co2V2O7) hexagonal nanosheets on nickel foam are manufactured by a facile hydrothermal method and then transformed into numerous smaller size interconnected hierarchical nanosheets without any shape change via electrochemical reconstruction. Benefiting from the favorable architecture of hierarchical nanosheets via electrochemical reconstruction, the Co2V2O7 hexagonal nanosheet electrode exhibits a remarkable long cycling performance with 272% specific capacitance retention after 100,000 cycles at a current density of 5 A g-1 and then displays an increasing specific capacitance of 1834 F g-1 (tested at 1 A g-1). Furthermore, an aqueous hybrid supercapacitor device based on the Co2V2O7 hexagonal nanosheet electrode exhibits a high energy density of 35.2 Wh kg-1 at a power density of 1.01 kW kg-1 and an excellent cyclic stability with 71.4% capacitance retention after 10,000 cycles at 5 A g-1. These results offer a practicable pathway for enhancing the electrochemical properties of other metal oxides through electrochemical reconstruction.
H2O-H-Na co-intercalation is beneficial to enhance the structure of open framework V-based materials, leading to the improvement of Zn2+ transport kinetics and solid-state solubility in V-based electrodes. Zn|Zn(CF3SO3)2|HNaV6O16·4H2O exhibits an excellent specific capacity of 432.4 mA h g-1 and a long-term cyclic performance up to 800 cycles at 2.0 A g-1 with a reversible phase transformation. For the fast charge capability, a high specific capacity of 411.2 mA h g-1 is achieved with a coulomb efficiency of 100%. The co-intercalation structure can provide a new strategy for the design of fast charge cathode materials in aqueous zinc ion batteries (AZIBs).
Transcatheter arterial chemoembolization (TACE) has been widely used in clinical practice as a first-line treatment for unresectable hepatocellular carcinoma (HCC). However, the current therapeuticeffect of TACE is far from satisfactory and thus requires further improvement. TACE combined with multifunctional magnetic particles may be a promising approach for the treatment of HCC. In this study, we designed a new magnetic drug carrier system consisting of micron-sized iron powder, barium ferrite (BaFe12O19), and carbon-coated iron nanocrystals (CCINs). CCINs possess properties, such as high drug loading and sustained release. BaFe12O19 could attract both CCINs and iron powder to form larger clusters after magnetization. Altogether, the triple therapeutic effects of chemotherapeutic enhancement, embolization, and thermal ablation could be realized herein. Further experiments indicate that the system has a high drug-loading capacity, good controlled-release effect, and no significant cytotoxicity. Under the action of a medium-frequency magnetic induction device, the magnetic induction temperature could reach 43 °C in one min while the maximum temperature of 70.8 °C could be reached in 2.5 h. Overall, this new carrier system displayed excellent antitumor effects in a mouse model. Our findings demonstrate the great application prospects of this system in TACE for HCC.
Carcinoma, Hepatocellular , Chemoembolization, Therapeutic , Liver Neoplasms , Nanoparticles , Animals , Barium , Barium Compounds , Carbon , Carcinoma, Hepatocellular/drug therapy , Combined Modality Therapy , Ferric Compounds , Iron , Liver Neoplasms/drug therapy , Mice
OBJECTIVE: To study clinical efficacy of autologous bone graft for acetabular defect of Crowe III and IV hip dysplasia. METHODS: The 22 patients with 25 hips of DDH (Crowe type III, IV) from March 2010 to May 2013 were retrospectively analyzed. Total hip arthroplasty (THA) combined autogenous bone grafting was performed for all these patients with osteoarthritis secondary to DDH. Among them, 19 patients were females (21 hips) and 3 patients were males (4 hips), ranging in age from 43 to 67 years old, averaged 55 years old. There were 6 hips with Crowe type III and 19 hips with Crowe type IV. Before surgery, all the patients had hip pain, limb shortening and hip limited function of hip joint. After 12 months, the degree of recovery about limb length, functional recovery, autogenous bone graft fusion were observed. RESULTS: All the patients were followed up and no dislocation were occurred. At 12 months after operation, the average Harris hip joint llzncation score were 83.30±6.13, and 18 cases got an excellent result and 4 good. The length of lower limbs decreased from preoperative (3.20±0.81) cm to 12 months after operation (0.92±0.23) cm (t=14.864, P<0.05). CONCLUSION: THA combined with structural femoral head autograft for patients with osteoarthritis secondary to DDH can obtain favorable results, significantly improving the effect of operation treatment.
Acetabulum/surgery , Femur Head/transplantation , Hip Dislocation, Congenital/surgery , Osteoarthritis/surgery , Adult , Aged , Arthroplasty, Replacement, Hip , Female , Humans , Male , Middle Aged , Transplantation, Autologous
OBJECTIVE: To observe clinical efficacy of renovation stem revision femoral head arthroplasty for the treatment of unstable intertrochanteric fracture in the elderly. METHODS: Totally 32 elderly patients with unstable intertrochanteric fracture were treated with renovation stem revision femoral head arthroplasty from September 2007 to January 2011. There were 11 males and 21 females with an average age of 83.8 (ranged, 80 to 98) years old,the time from injury to hospital ranged from 4 h to 14 days. According to Evans-Jensen classification, 6 cases were type II a, 20 cases were type II b, and 6 cases were type III. Postoperative mortality, complication rates and Harris hip function score were compared and analyzed to evaluate curative effect. RESULTS: All patients were followed up and no dislocation occurred. Six patients were died during 15 months and 4.5 years; 24 cases recoved to independent wakling at 6 months after operation, and 8 cases walked with stick and walker. The average Harris hip joint function score were (91.56 +/- 2.96), 28 cases got excellent results and 4 cases good. Nine cases occurred complications and healed after treatment. CONCLUSION: Renovation stem revision femoral head arthroplasty is a active and reliable method in treating unstable intertrochanteric fracture in the elderly.
Arthroplasty, Replacement, Hip/methods , Femoral Neck Fractures/surgery , Hip Fractures/surgery , Aged, 80 and over , Female , Humans , Male
This paper was designed to study metabonomic characters of the osteoporosis induced by high dose of hydrocortisone and the protective effects of Drynariae Rhizoma, which can replenish the kidney and strengthen the bones. A urinary metabonomics method based on ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) was developed. Clear separation of healthy control group, model group and treatment group was achieved by using the principal components analysis (PCA) and 9 significantly changed metabolites were identified as potential biomarkers of osteoporosis. Compared with the health control group, the model group rats showed lower levels of creatinine, citric acid, azelaic acid, hippurate, tryptophan and indoxyl sulfate together with higher levels of phenylalanine, cresol sulfate and phenaceturic acid. These changes in urinary metabolites suggest that the disorders of amino acid metabolism, energy metabolism, gut microflora and anti-oxidative damage are related to osteoporosis induced by high dose of hydrocortisone and the potential effect of Drynariae Rhizoma on all the four metabolic pathways.
Metabolomics , Osteoporosis/prevention & control , Plant Extracts/pharmacology , Polypodiaceae , Animals , Chromatography, High Pressure Liquid , Male , Osteoporosis/urine , Rats , Rats, Wistar , Tandem Mass Spectrometry
ETHNOPHARMACOLOGICAL RELEVANCE: Rhizoma Drynariae (RD) is an effectively traditional Chinese medicine which is usually used in treating osteoporosis, bone fracture, streptomycin ototoxicity and hyperlipemia. Up to now, studies on pharmacological mechanism of RD mostly focus on cell and gene level, little is known about its metabonomics study. The aim of this study is to establish the rats plasma metabonomic profiles of control, model and treatment group, then to investigate the anti-osteoporosis effect of RD and its action mechanism. METHOD: A total of 21 Wistar rats was divided into three groups: control group, model group and treatment group. The model and treatment rats were injected prednisolone for 12 weeks, at the same time the treatment rats were orally administered RD extract at a therapeutic dose (10g/kg, expressed as the weight of raw material) once daily throughout the experimental period, control group and model group were orally gavaged approximately volume normal saline solution. After 12 weeks, all plasma samples of three groups were collected and their metabolic profiling changes were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The resulting dataset was analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The identification of all potential biomarkers was performed using reference standard by comparing their mass spectra, MS/MS fragmentation and retention time. Furthermore, clinical biochemistry and biomechanics study were also carried out to ensure the success of the osteoporosis model and to investigate the anti-osteoporosis effect of RD. RESULTS: Obvious separation trend between control and model group was found in PCA score plot, the anti-osteoporosis effect of RD can be indicated in PLS-DA score plot among these three groups. Six potential metabolite biomarkers, Lysophosphatidylcholines (C16:0 LPC, C18:0 LPC, C18:1 LPC and C18:2 LPC), tryptophane and phenylalanine, which were proved to be related with osteoporosis, were identified in the rats plasma. Compared with control group, level of all biomarkers increased significantly in model group, while that was much closer to normal in treatment group. CONCLUSION: The anti-osteoporosis effect of RD has been reliably confirmed by the metabonomics method. The osteoporosis might be prevented by RD via intervening antioxidant-oxidation balance, tryptophane metabolism and phenylalanine metabolism in vivo in rats.
Bone Density Conservation Agents/therapeutic use , Drugs, Chinese Herbal/therapeutic use , Osteoporosis/drug therapy , Polypodiaceae , Alkaline Phosphatase/blood , Animals , Biomarkers/blood , Bone Density/drug effects , Chromatography, Liquid/methods , Disease Models, Animal , Femur/drug effects , Femur/physiology , Lysophosphatidylcholines/blood , Metabolomics , Osteocalcin/blood , Osteoporosis/blood , Osteoporosis/chemically induced , Osteoporosis/physiopathology , Phenylalanine/blood , Prednisolone , Protein Carbonylation , Rats , Rats, Wistar , Rhizome , Tandem Mass Spectrometry , Tibia/drug effects , Tibia/physiology , Tryptophan/blood
A high performance liquid chromatographic (HPLC) method using cellulose tris-(3,5-dimethylphenylcarbamate) as chiral stationary phase (Lux Cellulose-1) for the separation of trantinterol hydrochloride enantiomers was developed. The method was based on normal phase model with n-heptane as the basal solvent of mobile phase. The influences of organic modifiers, mobile phase additives and column temperature on the retention and separation of the enantiomers were examined and discussed. It was demonstrated that the mobile phase additives had a dominant effect on the enantiomeric separation. No separation was observed with the addition of diethylamine only in the mobile phase, while the retention was increased and the enatiomeric separation was observed with the addition of trifluoroacetic acid. When both trifluoroacetic acid and diethylamine were added into the mobile phase, the enantioseparation was significantly improved with a resolution up to 4.0. Ethanol and isopropanol were investigated as the organic modifiers, and ethanol offered a better enatiomeric resolution for trantinterol hydrochloride. In the examined temperature range between 15 degrees C and 35 degrees C both separation factor and resolution were decreased with the increase of the column temperature. The optimized chiral HPLC method for the separation of trantinterol hydrochloride enantiomers involved a Lux Cellulose-1 column (250 mm x 4.6 mm, 5 microm), a mobile phase of n-heptane/ethanol/trifluoroacetic acid/diethylamine (88: 12: 0.3: 0.05, v/v/v/v) at a flow rate of 1.0 mL/min, a detection at 246 nm and a column temperature of 25 degrees C. The method is simple and rapid for the enantiomeric impurity determination of (-)-trantinterol hydrochloride bulk samples.
Cellulose/analogs & derivatives , Chromatography, High Pressure Liquid/methods , Clenbuterol/analogs & derivatives , Cellulose/chemistry , Clenbuterol/chemistry , Clenbuterol/isolation & purification , Stereoisomerism
The success of gene therapy depends largely on the efficacy of gene delivery vector systems that can deliver genes to target organs or cells selectively and efficiently with minimal toxicity. Here, we show that by using the HRE.ppET-1 regulatory element, we were able to restrict expression of the transgene of vascular endothelial growth factor (VEGF) to endothelial cells exclusively in hypoxic conditions. Eukaryotic expression vectors such as pEGFP-HRE.ppET-1, pcDNA3.1-VEGF+Pa, pcDNA3.1-ppET-1+ EGF+Pa, and pcDNA3.1-HRE.ppET-1+VEGF+Pa were constructed by using a series of nuclear molecule handling methods like PCR, enzyme digestion. The recombinant vectors were transfected into HUVEC cells and HL7702 cells by the lipofectin method. GFP expression was observed with a fluorescence microscope to validate the specificity of expression in endothelial cells under the regulation of HRE.ppET-1 element. Cobalt chloride (final concentration 100 mumol/L) was added to the medium to mimic hypoxia in vitro. After transfection of vectors, the expression of VEGF mRNA was detected by RT-PCR, and the expression of VEGF was detected by Western blotting and ELISA methods under normoxia and hypoxia, respectively. The cell proliferation rate was detected by the MTT test. The expression of GFP revealed that the exterior gene was transcripted effectively in endothelial cells regulated by the HRE.ppET-1 element, while the expression of GFP was very weak in nonendothelial cells. The results of RT-PCR, Western blotting and ELISA showed that VEGF gene expression in the pcDNA3.1-HRE.ppET-1+VEGF+Pa group and in the pcDNA3.1-ppET-1+VEGF+Pa group was higher in hypoxia than it was in normoxia (P<0.05). The MTT test showed that the proliferation rate of HUVEC transfected with HPVA under hypoxia exceeded that of the control group. We conclude that the HRE.ppET-1 element was expressed specifically in endothelial cells, and can increase the expression of VEGF in hypoxia and stimulate proliferation of endothelial cells. Taking advantage of these facts could greatly improve the efficiency of gene therapy. The vector would be valuable for various gene transfer studies targeting endothelial cells.
Endothelial Cells/metabolism , Genetic Vectors , Vascular Endothelial Growth Factor A/genetics , Base Sequence , Cell Hypoxia , Cell Proliferation , Cells, Cultured , DNA Primers/genetics , Endothelial Cells/cytology , Endothelin-1/genetics , Genetic Therapy , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Humans , Promoter Regions, Genetic , RNA, Messenger/genetics , RNA, Messenger/metabolism , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Transcriptional Activation , Transfection , Vascular Endothelial Growth Factor A/metabolism
In the title complex, [Cu(C(11)H(9)ClN(3)O(2))(2)]·4H(2)O, the Cu(II) atom is in a distorted octa-hedral coordination environment, coordinated by four N atoms and two O atoms from two tridentate 3-chloro-6-(3,5-dimethyl-1H-pyrazol-1-yl)picolinate ligands. The mol-ecules are linked via inter-molecular O-Hâ¯O hydrogen bonds involving water mol-ecules to form extended chains along [010], and there are short Clâ¯Cl contacts [3.153â (4)â Å].
