Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(l-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity.

PURPOSE: Nanoparticle (NP)-based drug delivery approaches have tremendous potential for enhancing treatment efficacy and decreasing doses of chemotherapeutics. Idarubicin (IDA) is one of the most common chemotherapeutic drugs used in the treatment of acute myeloid leukemia (AML). However, severe side effects and drug resistance markedly limit the application of IDA. METHODS: In this study, we encapsulated IDA in polymeric NPs and validated their antileukemia activity in vitro and in vivo. RESULTS: NPs with an average diameter of 84 nm was assembled from a methoxy poly(ethylene glycol)-b-poly(l-lactide-co-glycolide) (mPEG-PLGA). After loading of IDA, IDA-loaded mPEG-PLGA NPs (IDA/mPEG-PLGA NPs) were formed. The in vitro release data showed that the IDA/mPEG-PLGA NPs have excellent sustained release property. IDA/mPEG-PLGA NPs had exhibited the lower IC50 than pure IDA. Moreover, IDA/mPEG-PLGA NPs in the same concentration substantially induced apoptosis than did pure IDA. Most importantly, IDA/MPEG-PLGA NPs significantly decreased the infiltration of leukemia blasts and improved the overall survival of MLL-AF9-induced murine leukemia compared with free IDA. However, the blank NPs were nontoxic to normal cultured cells in vitro, suggesting that NPs were the safe carrier. CONCLUSION: Our data suggest that IDA/mPEG-PLGA NPs might be a suitable carrier to encapsulate IDA. Low dose of IDA/mPEG-PLGA NPs can be used as a conventional dosage for antileukemia therapy to reduce side effect and improve survival.

Skelate changes induced by orthodontic in class II division 1 by CBCT: a long-term follow-up prospective study.

OBJECTIVE: To identify and evaluate changes in the sagittal position of point B due to orthodontic treatment using CBCT. MATERIALS AND METHODS: The subjects comprised 80 patients received fixed appliance. In this population, group 1 consisting of 40 patients with Class II division 2 malocclusion and group 2 consisting of 40 patients with minor crowding in the beginning of the treatment and required no or minimal maxillary anterior tooth movement. Treatment changes in incisor inclination, sagittal position of point B, SNB and movement of incisor root apex and incisal edge were calculated on pretreatment and post treatment on CBCT. RESULTS: Assessment of local changes in point B revealed that the point had moved backward. No significant change was observed in the value of the sella-nasion-point B angle (SNB) in both the study and control groups. However, the changes of horizontal displacement after treatment in SNB between the two groups were found to be significant. There were no significant changes in the vertical and Z position of points B in both group. CONCLUSIONS: The position of point B was affected by local bone remodeling during orthodontic treatment. These changes significantly affect the SNB angle.

[Soft tissue changes of upper lip in patients with class I malocclusion with different premolar extraction patterns].

OBJECTIVE: The purpose of this retrospective cephalometric study was to compare changes of the upper lip soft tissue in patients with Class I malocclusion after extraction of either first premolars or second premolars. METHODS: The pretreatment and posttreatment records of 50 patients were used in the study. And 25 patients involved extraction of first premolars and 25 patients involved the extraction of second premolars. The lateral cephalometric radiographs taken before and after treatment were traced and measured. 15 typical values of soft tissue were measured in Winceph 7.0 software and statistical analysis was carried by SPSS 10.0 software. RESULTS: 1) The horizontal and vertical lines were not significant different in the two extraction group before the treatment. But after the treatment the vertical changes of upper lip (A'-X, UL-X) were more apparent in the second premolar extraction group (P < 0.05). 2)In the soft tissue measurements, the values of Sn-A'-UL in the first premolar extraction group were less than those in the second premolar extraction group significantly before the treatment. After the treatment all the patients had a significant increase in length of upper lip. At the same time, UL-EP, A'-EP distances were diminished obviously (P < 0.05). But the changes were not significant difference between the two premolar extraction groups (P > 0.05). CONCLUSION: The upper lips both changed remarkably after the treatment and there was no significant difference between the two premolar extraction groups.

[Soft tissue changes of chin with different premolar extraction patterns].

PURPOSE: The purpose of this retrospective cephalometric study was to compare the change of chin soft tissue in patients with Class I malocclusion after extraction of either first premolars or second premolars. METHODS: The pretreatment and posttreatment records of 50 patients were used in the study. Twenty-five patients underwent extraction of first premolars and 25 patients underwent extraction of second premolars. All the patients were skeletal Class I and matched by the gender, age, facial divergence and crowding. The lateral cephalometric radiographs taken before and after treatment were traced and measured. The data was analyzed using SPSS 10.0 software package for Student's t test. RESULTS: (1)The horizontal and vertical changes was both significant in the second extraction group compared with first extraction group, and the horizontal change was more apparent. But only vertical change was apparent in the first extraction group. (2)In the soft tissue measurements, the value of soft tissue facial angle and B'-Pos-FH in the first extraction group was less than those in the second extraction group significantly. After treatment all the patients had an increase in S-Ns-Pos angle, soft tissue facial angle, B'-Pos-FH and depth of mentolabial groove. At the same time, soft tissue convexity angle was diminished obviously. But the change was not significant between the two premolar extraction groups. CONCLUSIONS: The growth pattern tends to vertical pattern in the first premolar extraction group and the horizontal growth of chin is more obvious in the second premolar extraction group. There is no significant difference of chin soft tissue changes between the two premolar extraction groups.