OBJECTIVES: Chronic low back pain (CLBP) can seriously impair the quality of life of patients and has a remarkable comorbidity with psychological symptoms, which, in turn, can further exacerbate the symptoms of CLBP. Psychological treatments are critical and nonnegligent for the management of CLBP, and thus, should attract sufficient attention. However, current evidence does not suggest the superiority and effectiveness of nonpharmacological interventions in reducing psychological symptoms among patients with CLBP.Thus, this study was designed to compare the effectiveness of nonpharmacological interventions for depression, anxiety, and mental health among patients with CLBP and to recommend preferred strategies for attenuating psychological symptoms in clinical practice. METHODS: In this systematic review and network meta-analysis (NMA), PubMed, Embase Database, Web of Science, and Cochrane Library were searched from database inception until March 2022. Randomized clinical trials (RCTs) that compare different nonpharmacological interventions for depression, anxiety, and mental health among patients with CLBP were eligible. The Preferred Reporting Items for Systematic Reviews and Meta-analyses statement was used. Four reviewers in pairs and divided into two groups independently performed literature selection, data extraction, and risk of bias, and certainty of evidence assessments. This NMA was conducted with a random effects model under a frequentist framework. The major outcomes were depression, anxiety, and mental health presented as the standardized mean difference (SMD) with the corresponding 95% CI. RESULTS: A total of 66 RCTs that randomized 4806 patients with CLBP met the inclusion criteria. The quality of evidence was typically low or some risks of bias (47 out of 66 trials, 71.3%), and the precision of summary estimates for effectiveness varied substantially. In addition, 7 categories of interventions with 26 specific treatments were evaluated. For depression, mind body therapy (pooled SMD = -1.20, 95% CI: -1.63 to -0.78), biopsychosocial approach (pooled SMD = -0.41, 95% CI: -0.70 to -0.12), and physical therapy (pooled SMD = -0.26, 95% CI: -0.50 to -0.02) exhibited remarkable effectiveness in reducing depression compared with the control group. For managing anxiety, mind body therapy (pooled SMD = -1.35, 95% CI: -1.90 to -0.80), multicomponent intervention (pooled SMD = -0.47, 95% CI: -0.88 to -0.06), and a biopsychosocial approach (pooled SMD = -0.46, 95% CI: -0.79 to -0.14) were substantially superior to the control group. For improving mental health, multicomponent intervention (pooled SMD = 0.77, 95% CI: 0.14 to 1.39), exercise (pooled SMD = 0.60, 95% CI: 0.08 to 1.11), and physical therapy (pooled SMD = 0.47, 95% CI: 0.02-0.92) demonstrated statistically substantial effectiveness compared with the control group. The rank probability indicated that mind body therapy achieved the highest effectiveness in reducing depression and anxiety among patients with CLBP. Besides, the combined results should be interpreted cautiously based on the results of analyses evaluating the inconsistency and certainty of the evidence. CONCLUSION: This systemic review and NMA suggested that nonpharmacological interventions show promise for reducing psychological symptoms among patients with CLBP. In particular, mind body therapy and a biopsychosocial approach show considerable promise, and mind body therapy can be considered a priority choice in reducing depression and anxiety. These findings can aid clinicians in assessing the potential risks and benefits of available treatments for CLBP comorbidity with psychological symptoms and provide evidence for selecting interventions in clinical practice. More RCTs involving different interventions with rigorous methodology and an adequate sample size should be conducted in future research.
Low Back Pain , Humans , Low Back Pain/therapy , Anxiety/etiology , Anxiety/therapy , Comorbidity , Quality of Life
To investigate the responses of resin canal area of Pinus tabuliformis with different ages to climate change, we analyzed the relationship between the resin canal area and climate factors from 1972 to 2017 in P. tabuliformis plantation at young age, middle age, near-mature, mature, and over-mature periods in Taiyue Mountains, Shanxi Province. The results showed that 55.7% to 75.2% of resin canal occurred in earlywood. The mature and over-mature periods had the largest resin channel area, sequentially followed by near mature, middle age, and young age periods. Annual mean latewood resin canal area increased significantly in near-mature period and mature period, but not in other periods. There was a significant negative correlation between earlywood resin canal area and minimum temperature during the growing season (May to July) for trees at middle age period. Earlywood resin canal area of mature trees showed significant negative correlation with minimum temperature during the growing season, but significant positive correlation with drought (PDSI) in the non-growing season (previous September to current April). The total area of earlywood resin canal at the young, near-mature, and mature periods was less influenced by climate than other periods. The minimum growing season temperature was a limiting factor to earlywood resin canal growth, while the non-growing season drought gradually became a limiting factor for earlywood resin canal with increasing tree age. The total area of earlywood resin canal decreased with increasing non-growing season drought and increasing growing season minimum temperature. In the context of global warming, the earlywood resin canal area may gradually decrease at all five periods, and the most reduction in the over-mature stage.
Climate Change , Pinus , Global Warming , China , Droughts , Trees
To understand the responses of radial growth to climatic factors and the differences in ecological resilience to drought between a heliophilous species Larix principis-rupprechtii and a shade species Picea meyeri in mixed forests, we developed the tree-ring width chronologies of L. principis-rupprechtii and P. meyeri in three mixed forests based on the samples collected from Toudaogou of Saihanba in Hebei, Ningwu County and Kelan County in Shanxi Province. We analyzed the correlation between climatic factors and various chronologies and examined the differences in resistance (Rc), recovery (Rt), and resilience (Rs) of L. principis-rupprechtii and P. meyeri in response to drought stress. The results showed that the radial growth of L. principis-rupprechtii and P. meyeri was negatively correlated with the mean and maximum air temperature from May to July in three mixed forests, and was positively correlated with the Palmer drought index (PDSI) from May to September. Radial growth decline in trees due to drought stress was significantly different between the two species among the three sites, indicating different physiological and ecological regulation strategies. The resistance of P. meyeri was stronger than that of L. principis-rupprechtii at the three study sites, with stronger resilience and resilient elasticity of L. principis-rupprechtii than P. meyeri. As a result, P. meyeri exhibited greater drought resistance than L. principis-rupprechtii. Under global warming condition, L. principis-rupprechtii might be at greater risk of growth decline than P. meyeri in this region.
Larix , Picea , Droughts , Drought Resistance , Forests , Trees
Zinc isotopic ratios serve as powerful tools for tracing biochemical cycling of metals at Earth's surface, including the distribution, transportation, and enrichment of zinc (Zn) in soil. To conduct such studies and enable inter-laboratory comparisons, high-precision Zn isotopic measurements require the use of soil reference materials (RMs). However, there have been limited reports on the high-precision Zn isotope ratios of soil RMs thus far. In this study, we have developed a two-step Zn chemical separation protocol utilizing Bio-Rad AG MP-1M resin columns. This method has demonstrated excellent reproducibility for measuring the external δ66Zn values (relative to JMC-Lyon) of standard soil reference materials over an extended time period, with a better than 0.06 (2SD) precision. Remarkably, this study is the first to report the Zn isotopic compositions of 20 soil reference materials from various soil types in China. With the exception of one sample obtained from a mining area, the Zn isotopic compositions of all the analyzed soil reference materials exhibit remarkable similarity, with an average δ66Zn value of 0.31 ± 0.12, which aligns closely with the values observed in igneous rocks. The exceptional sample, with a higher δ66Zn value of 0.61 ± 0.02, indicates potential contamination during mining activities.
OBJECTIVE: Hounsfield units (HU), vertebral bone quality (VBQ), and bone mineral density (BMD) can all serve as predictive indicators for thoracolumbar fragility fractures. This study aims to explore which indicator provides better risk prediction for thoracolumbar fragility fractures. METHODS: Patients who have received medical attention from The First Affiliated Hospital of Anhui Medical University for thoracolumbar fragility fractures were selected. A total of 78 patients with thoracolumbar fragility fractures were included in the study. To establish a control group, 78 patients with degenerative spinal diseases were matched to the fracture group on the basis of gender, age, and body mass index. The lumbar vertebral HU, the VBQ, and the BMD were obtained for all the 156 patients through computed tomography, magnetic resonance imaging, and dual-energy x-ray absorptiometry (DEXA). The correlations among these parameters were analyzed. The area under curve (AUC) analysis was employed to assess the predictive efficacy and thresholds of lumbar vertebral HU, VBQ, and BMD in relation to the risk of thoracolumbar fragility fractures. RESULTS: Among the cohort of 156 patients, lumbar vertebral HU exhibited a positive correlation with BMD (p < 0.01). Conversely, VBQ showed a negative correlation with HU, BMD (p < 0.05). HU and BMD displayed a favorable predictive efficacy for thoracolumbar fragility fractures (p < 0.01), with HU (AUC = 0.863) showcasing the highest predictive efficacy, followed by the DEXA-measured BMD (AUC = 0.813). VBQ (AUC = 0.602) ranked lowest among the 3 indicators. The thresholds for predicting thoracolumbar fragility fractures were as follows: HU (88),VBQ (3.37), and BMD (0.81). CONCLUSION: All 3 of these indicators, HU, VBQ, and BMD, can predict thoracolumbar fragility fractures. Notably, lumbar vertebral HU exhibits the highest predictive efficacy, followed by the BMD obtained through DEXA scanning, with VBQ demonstrating the lowest predictive efficacy.
To analyze the effects of forest edge on radial growth and cell characteristics in different stand types of Larix principis-rupprechtii, we investigated the differences on radial growth, cell size and numbers between edge trees and inner trees of L. principis-rupprechtii in pure L. principis-rupprechtii forests and mixed forests of L. principis-rupprechtii and Betula platyphylla in Saihanba mechanical forest farm, China. The results showed that radial growth of the edge trees was significantly faster than that of the inner trees in pure forests, with the total ring width, earlywood width and latewood width of edge trees being 48.9%, 58.9% and 29.6% higher than those of inner trees, respectively. However, there was no difference in radial growth between edge trees and inner trees in mixed forest. The total number of earlywood cells, the number of large cells and small cells in earlywood of edge trees were increased by 63.3%, 55.6% and 70.0%, while the total number of latewood cells, the number of large cells and small cells in latewood of edge trees were increased by 35.4%, 37.5% and 28.5% compared with those of inner trees. There was no significant difference in the cell sizes between edge trees and inner trees. The cell numbers of earlywood and latewood of edge trees were not significantly different from those of inner trees in mixed forest, but the cell size in the earlywood of edge trees was 50.0% larger than those of inner trees in mixed forest. The sizes of the largest cells, the smallest cells, the large cells and the small cells in the earlywood of edge trees were increased by 28.6%, 33.3%, 16.6% and 25.0% compared with those of inner trees, respectively. The fast growth of edge trees and slow growth of inner trees in the pure forests could be effectively alleviated by cultivating mixed forests.
Larix , Betula , China , Forests , Trees
Gossypium hirsutum (upland cotton) is one of the most economically important crops worldwide, which has experienced the long terms of evolution and domestication process from wild species to cultivated accessions. However, nucleotide evolution, domestication selection, and the genetic relationship of cotton species remain largely to be studied. In this study, we used chloroplast genome sequences to determine the evolutionary rate, domestication selection, and genetic relationships of 72 cotton genotypes (36 cultivated cotton accessions, seven semi-wild races of G. hirsutum, and 29 wild species). Evolutionary analysis showed that the cultivated tetraploid cotton genotypes clustered into a single clade, which also formed a larger lineage with the semi-wild races. Substitution rate analysis demonstrated that the rates of nucleotide substitution and indel variation were higher for the wild species than the semi-wild and cultivated tetraploid lineages. Selection pressure analysis showed that the wild species might have experienced greater selection pressure, whereas the cultivated cotton genotypes underwent artificial and domestication selection. Population clustering analysis indicated that the cultivated cotton accessions and semi-wild races have existed the obviously genetic differentiation. The nucleotide diversity was higher in the semi-wild races compared with the cultivated genotypes. In addition, genetic introgression and gene flow occurred between the cultivated tetraploid cotton and semi-wild genotypes, but mainly via historical rather than contemporary gene flow. These results provide novel molecular mechanisms insights into the evolution and domestication of economically important crop cotton species.
Cotton is one of the most important fiber and oil crops in the world. Chloroplast genomes harbor their own genetic materials and are considered to be highly conserved. Transfer RNAs (tRNAs) act as "bridges" in protein synthesis by carrying amino acids. Currently, the variation and evolutionary characteristics of tRNAs in the cotton chloroplast genome are poorly understood. Here, we analyzed the structural variation and evolution of chloroplast tRNA (cp tRNA) based on eight diploid and two allotetraploid cotton species. We also investigated the nucleotide evolution of chloroplast genomes in cotton species. We found that cp tRNAs in cotton encoded 36 or 37 tRNAs, and 28 or 29 anti-codon types with lengths ranging from 60 to 93 nucleotides. Cotton chloroplast tRNA sequences possessed specific conservation and, in particular, the Ψ-loop contained the conserved U-U-C-X3-U. The cp tRNAs of Gossypium L. contained introns, and cp tRNAIle contained the anti-codon (C-A-U), which was generally the anti-codon of tRNAMet. The transition and transversion analyses showed that cp tRNAs in cotton species were iso-acceptor specific and had undergone unequal rates of evolution. The intergenic region was more variable than coding regions, and non-synonymous mutations have been fixed in cotton cp genomes. On the other hand, phylogeny analyses indicated that cp tRNAs of cotton were derived from several inferred ancestors with greater gene duplications. This study provides new insights into the structural variation and evolution of chloroplast tRNAs in cotton plants. Our findings could contribute to understanding the detailed characteristics and evolutionary variation of the tRNA family.
Evolution, Molecular , Genes, Chloroplast , Genomic Structural Variation , Gossypium/genetics , RNA, Transfer/genetics , Codon/genetics , Gossypium/classification , Phylogeny
BACKGROUND: Giant cell tumor (GCT) is a benign lesion and rarely involves the patella. This disease is characterized by a relatively high recurrence rate after primary treatment. En bloc resection has been a predominant option for recurrent GCT. However, total patellectomy can lead to disruption of the knee. Therefore, exploration of functional reconstruction of the extensor mechanism is worthwhile. CASE SUMMARY: A 54-year-old woman presented with right knee pain and swelling, and was diagnosed as having a GCT in the patella following curettage and autograft. Medical imaging revealed a lytic and expanded lesion involving the whole patella with focal cortical breaches and pathological fracture. Based on the combination of histological, radiological, and clinical features, a diagnosis of recurrent GCT in the patella was made (Campanacci grade III). After a multidisciplinary team discussion, three-dimensional (3D)-printed custom-made patellar endoprosthesis was performed following en bloc resection for reconstructing the extensor mechanism. The patient was followed for 35 mo postoperatively. No evidence of local recurrence, pulmonary metastasis, or osteoarthritis of the right knee was observed. The active flexion arc was 0°-120°, and no extension lag was detected. A favorable patellar tracking and height (Insall-Salvati ratio 0.93) were detected by radiography. CONCLUSION: We depict a case of a GCT at the right patella, which was successfully treated by patellectomy and 3D-printed custom-made endoprosthetic replacement. The patella normal reconstruction, the precise-fit articular design, and gastrocnemius flap augmentation could lead to satisfactory knee function and a low rate of complications in the short-term follow-up.
Mitochondrial dysfunction is a hallmark of Alzheimer's disease (AD), which may be related to mitophagy failure. Previous reports suggest that treadmill exercise protects against mitochondrial dysfunction in AD. However, few studies have investigated the relationship between mitophagy and mitochondrial adaptation caused by treadmill exercise in AD. The current study aimed to investigate whether exercise-ameliorated AD is associated with changes in mitophagy activity. Both Wild-type and APP/PS1 transgenic mice were divided into sedentary (WTC and ADC) and exercise (WTE and ADE) groups (n = 9 for each group). WTE and ADE mice were subjected to treadmill exercise for 12 weeks, followed by evaluating the effect of treadmill exercise on learning and memory ability, Aß plaques, mitochondrial Aß peptide level, synaptic activity and mitochondrial function. Meanwhile, mitophagy-related proteins PINK1, Parkin, LC3II and P62 were measured in the hippocampal mitochondrial fractions. The results indicated that exercise not only restored learning and memory ability, but also reduced Aß plaque area, mitochondrial Aß peptide level, and increased levels of synaptic markers SYN and GAP43, as well as reversed mitochondrial dysfunction (defective mitochondrial ultrastructure, decreased PGC-1α, TFAM and ATP levels) in APP/PS1 transgenic mice. Moreover, exercise increased mitophagy activity as evidenced by a significant decrease in levels of P62 and PINK1 as well as an increase in levels of LC3II and Parkin in ADE mice. These findings suggest that treadmill exercise can enhance mitophagy activity in the hippocampus, which is efficient in ameliorating pathological phenotypes of APP/PS1 transgenic mice.
Amyloid beta-Peptides/metabolism , Amyloid beta-Protein Precursor , Mitochondria/metabolism , Mitophagy/physiology , Physical Conditioning, Animal/physiology , Presenilin-1 , Amyloid beta-Peptides/antagonists & inhibitors , Amyloid beta-Protein Precursor/genetics , Animals , Exercise Test/methods , Male , Maze Learning/physiology , Mice , Mice, Inbred C57BL , Mice, Transgenic , Mitochondria/pathology , Physical Conditioning, Animal/methods , Presenilin-1/genetics
The deposition of amyloid-ß (Aß) is one of the major pathological hallmarks of Alzheimer's disease (AD), and a higher cholesterol level is involved in the deposition of Aß. Previous evidence suggested that exercise exerts neuroprotective effects in a variety of AD models. In the present study, we investigated the effects of a 12-week treadmill exercise program on Aß deposits in amyloid precursor protein (APP)/presenilin 1 (PS1) transgenic mice and the potential underlying mechanism. After 12 weeks of exercise, Aß deposits were significantly decreased in the hippocampus. Meanwhile, the amyloidogenic pathway of APP metabolism was inhibited, which was associated with a decrease in BACE1 expression. The APP metabolism mediated by the nonamyloidogenic pathway, as indicated by the increase in ADAM10 levels. Coincidentally, exercise reduced the cholesterol level, as evidenced by a significant decrease in the total cholesterol level and the activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, as well as a reduction in the number of lipid rafts, as evidenced by a significant decrease in the flotillin 1 level. These finding suggested that the 12-week treadmill exercise program reduced Aß deposition in the hippocampus of APP/PS1 mice, possibly by regulating ADAM10 and BACE1 levels and by decreasing cholesterol-mediated lipid raft formation, indicating that exercise represents a therapeutic intervention to treat AD.
Alzheimer Disease/metabolism , Amyloid beta-Peptides/biosynthesis , Hippocampus/metabolism , Membrane Microdomains/metabolism , Physical Conditioning, Animal/methods , Alzheimer Disease/pathology , Animals , Cholesterol/metabolism , Hippocampus/pathology , Male , Mice , Mice, Transgenic , Presenilin-1 , Random Allocation
Ravine and slope are the two typical micro-topographies in the Great Xing'an Mountains. Permafrost in the two micro-topographies is extremely sensitive to global warming. To compare the differences in responses of Larix gmelinii radial growth to rapid warming in the recent 30 years, tree-ring samples were collected from northern part of Great Xing'an Mountains in Inner Mongolia and tree ring-width chronologies were developed. The results showed that the divergence between tree growth and temperature occurred in 1980 in Mo'erdaoga and in 2000 in Hanma, respectively. Since 2000, tree growth on ravine sites was greater than that on the slope stands. Chronology of two topographies reached significant level by t test in 1984-2008, the Euclidean distance was 0.937-2.742 between chronologies, and far greater than the average (0.809-1.304). The rapid warming caused a significant radial growth response in L. gmelinii to hydrothermal conditions. On the slope sites, tree growth was mainly controlled by temperature and declined gradually in recent years, while the trees growing in ravines showed an accelerating growth rate with permafrost degeneration. L. gmelinii was undergoing profound environmental pressures caused by rapid warming, and it would experience a complex growth and development process. More seriously, L. gmelinii might disappear in China with a warming climate and permafrost degeneration.
Global Warming , Larix/growth & development , China , Climate , Temperature , Trees
Based on BIOME-BGC model and tree-ring data, a modeling study was conducted to estimate the dynamic changes of the net primary productivity (NPP) of Pinus tabulaeformis forest ecosystem in North China in 1952-2008, and explore the responses of the radial growth and NPP to regional climate warming as well as the dynamics of the NPP in the future climate change scenarios. The simulation results indicated the annual NPP of the P. tabulaeformis ecosystem in 1952-2008 fluctuated from 244.12 to 645.31 g C x m(-2) x a(-1), with a mean value of 418.6 g C x m(-2) x a(-1) The mean air temperature in May-June and the precipitation from previous August to current July were the main factors limiting the radial growth of P. tabulaeformis and the NPP of P. tabulaeformis ecosystem. In the study period, both the radial growth and the NPP presented a decreasing trend due to the regional warming and drying climate condition. In the future climate scenarios, the NPP would have positive responses to the increase of air temperature, precipitation, and their combination. The elevated CO2 would benefit the increase of the NPP, and the increment would be about 16.1% due to the CO2 fertilization. At both ecosystem and regional scales, the tree-ring data would be an ideal proxy to predict the ecosystem dynamic change, and could be used to validate and calibrate the process-based ecosystem models including BIOME-BGC.
Carbon Dioxide/analysis , Climate Change , Ecosystem , Pinus/physiology , Plant Stems/anatomy & histology , China , Computer Simulation , Models, Biological , Pinus/growth & development
OBJECTIVE: To study the clinicopathologic and immunohistochemical features of mesenchymal chondrosarcoma. METHODS: The clinical and histologic features of 23 cases of mesenchymal chondrosarcoma were analyzed. Immunohistochemical study was also performed in 14 of the cases. RESULTS: The age of patients ranged from 12 to 47 years. Fourteen of them occurred in males. Thirteen cases involved the bony skeleton and 5 cases affected the soft tissue. The patients presented with pain and/or swelling. Histologically, the tumor consisted of a mixture of undifferentiated small round cells and hyaline cartilage. Transition between the two components was demonstrated and growth plate-like cartilage was observed. Immunohistochemical study showed that the small round cells were positive for Sox9 (14/14), CD99 (12/14), vimentin (6/14), CD56 (4/14), CD57 (4/14), neuron-specific enolase (3/14) and desmin(1/14). They were negative for Coll-II, S-100 protein, epithelial membrane antigen, pan-cytokeratin, synaptophysin, chromogranin A, CD34 and c-erbB2. CONCLUSIONS: Mesenchymal chondrosarcoma is a rare malignant tumor. Thorough histologic examination, when coupled with immunohistochemical findings, is helpful in arriving at a correct diagnosis.
Bone Neoplasms/pathology , Chondrosarcoma, Mesenchymal/pathology , SOX9 Transcription Factor/metabolism , 12E7 Antigen , Adolescent , Adult , Antigens, CD/metabolism , Bone Neoplasms/diagnostic imaging , Bone Neoplasms/metabolism , Bone Neoplasms/surgery , Cell Adhesion Molecules/metabolism , Child , Chondrosarcoma, Mesenchymal/diagnostic imaging , Chondrosarcoma, Mesenchymal/metabolism , Chondrosarcoma, Mesenchymal/secondary , Chondrosarcoma, Mesenchymal/surgery , Female , Follow-Up Studies , Humans , Immunohistochemistry , Lung Neoplasms/secondary , Male , Mediastinal Neoplasms/diagnostic imaging , Mediastinal Neoplasms/metabolism , Mediastinal Neoplasms/pathology , Mediastinal Neoplasms/surgery , Middle Aged , Neoplasm Recurrence, Local , Orbital Neoplasms/diagnostic imaging , Orbital Neoplasms/metabolism , Orbital Neoplasms/pathology , Orbital Neoplasms/surgery , Radiography , Vimentin/metabolism , Young Adult
OBJECTIVE: To study the effects of urokinase intervention on endotoxin-induced DIC in Wistar rats model. METHODS: Wistar rats were randomly assigned into 4 groups: normal saline (NS) group, urokinase (UK) group, endotoxin (LPS) group and LPS+ UK group. These agents were given to the rats by the tail vein intravenous infusion, NS group was treated with NS 2.5 mL/h x 4 h; UK group with NS 2.5 mL/h, 1 h later UK 4 IU/(g x h) x 3 h; LPS group with LPS 3 mg/(kg x h) x 4 h; LPS+UK group with LPS 3 mg/(kg x h) firstly, 1 h later UK 4 IU/(g x h) ) < 3 h. After the intervention, the function of coagulation and fibrinolysis, the indicators of organ damage and microcirculation fibrin micro-clots were evaluated. RESULTS: One hour after the infusion of 3 mg/(kg x h) of LPS, DIC signs began to appear, and became more apparent over time. After the intervention of urokinase, the values of clotting time (PT), activated partial thromboplastin time (APTT) were significantly shorter, but the platelet count (PLT), the amount of fibrinogen (FIB) changed little. Plasminogen activator inhibitor-1 (PAI-1) level decreased, while the D-dimer level increased. Serum creatinine (Cr), alanine aminotransferase (ALT) also decreased significantly. The biopsy of liver, kidney, and lung showed tissue damage became better, and the organ microcirculation fibrin micro-clots decreased significantly. CONCLUSION: The concentration of 3 mg/(kg x h) endotoxin can successfully induce DIC model in Wistar rats. Urokinase could play a positive role to prevent the LPS-induced DIC.
Disease Models, Animal , Disseminated Intravascular Coagulation/chemically induced , Disseminated Intravascular Coagulation/prevention & control , Endotoxins , Urokinase-Type Plasminogen Activator/therapeutic use , Animals , Female , Random Allocation , Rats , Rats, Wistar , Urokinase-Type Plasminogen Activator/pharmacology
Based on the established tree-ring width chronology of Larix gmelinii in Kuduer, Great Xing' an Mountains, this paper analyzed the relationships between L. gmelinii tree-ring width chronology and related climatic variables, including air temperature, precipitation, and PDSI. In the study area, the L. gmelinii tree-ring width chronology was significantly negatively correlated with the air temperature in May and July (P<0.01), had no significant correlation with precipitation, but significantly positively correlated with the PDSI in June-August (P<0.05), suggesting that hydro-thermal variables had significant coupling effects to the radial growth of L. gmelinii in its growth season, particularly in May and July. The L. gmelinii tree-ring width chronology had significant correlations with large scale climatic regimes such as the Pacific Decadal Oscillation (PDO) in both low and high frequency variations, illustrating the significant effects of the remote oceanic climatic regimes to the local tree growth.
Climate Change , Larix/anatomy & histology , Larix/growth & development , Plant Stems/anatomy & histology , China , Chronobiology Phenomena
BACKGROUND: Myofibroblastic sarcoma was used to be a controversial neoplasm. This study investigated the clinicopathological features of 20 cases of myofibroblastic sarcoma arising in different locations. METHODS: The paraffin-embedded tissue samples from 20 cases of patients with myofibroblastic sarcoma were stained immunohistochemically, and 5 cases examined by electron microscopy. Student's t test was used to analyze the difference of Ki-67 labeling index between grade 1 and grade 2 myofibroblastic sarcomas. RESULTS: Histologically, the tumors were composed of slender spindle cells with eosinophilic cytoplasm, and fusiform, tapering, wavy, or plump ovoid; vesicular nuclei and a small central eosinophilic nucleoli. Immunohistochemically, the tumor cells expressed smooth muscle actin (18/20), muscle specific actin (16/20), fibronectin (20/20) and desmin (2/20). Ultrastructurally, the tumor cells revealed abundant rough endoplasmic reticulum and longitudinally arranged fine filaments with focal densities in the cytoplasm. A clinical follow-up of 19 patients showed that 2 cases experienced local recurrence and distant metastasis 6 months to 4 years after the initial operation. Nine cases recurred locally 17 to 46 months after the initial excision, and 9 cases were alive with no evidence of disease. CONCLUSIONS: Myofibroblastic sarcomas, which exhibit diverse histological appearance, can easily be misdiagnosed as benign tumors. Myofibroblastic sarcomas are local destructive lesions with frequent recurrence, and may metastase distantly.
Fibrosarcoma/pathology , Myosarcoma/pathology , Soft Tissue Neoplasms/pathology , Adolescent , Adult , Child , Desmin/analysis , Female , Fibrosarcoma/chemistry , Humans , Immunohistochemistry , Male , Middle Aged , Myosarcoma/chemistry , Recurrence , Soft Tissue Neoplasms/chemistry
OBJECTIVE: We describe the clinicopathologic features of 6 cases of myofibroblastic sarcoma (MS) occurring in the nasal cavity and paranasal sinus. STUDY DESIGN: The paraffin-embedded tissues of 6 cases of MS were stained immunohistochemically and examined by electron microscopy. RESULTS: Clinically, a painless enlarging mass was the most common symptom, followed by the nasal obstruction, epistaxis, copious rhinorrhea, and proptosis. Histologically, the tumors showed a diffusely infiltrative growth pattern and consisted mainly of spindle cells with abundant eosinophilic cytoplasm. The hypocellular myxoid areas and the hypercellular fibrous areas were identified. Immunohistochemically, all 6 tumors were positive for vimentin, alpha-smooth muscle actin, calponin, and fibronectin. Ultrastructural examination in 3 cases showed characteristic features of myofibroblast. Follow-up in 6 patients revealed high local recurrence rate (6 out of 6). CONCLUSION: Myofibroblastic sarcoma of the nasal cavity and paranasal sinus exhibit diverse histologic appearances and a strong aggressive behavior.
Myosarcoma/pathology , Nose Neoplasms/pathology , Paranasal Sinus Neoplasms/pathology , Actins/analysis , Adolescent , Adult , Aged , Calcium-Binding Proteins/analysis , Fatal Outcome , Female , Fibronectins/analysis , Humans , Immunoenzyme Techniques , Male , Microfilament Proteins/analysis , Middle Aged , Myosarcoma/chemistry , Myosarcoma/ultrastructure , Neoplasm Invasiveness , Nose Neoplasms/chemistry , Nose Neoplasms/ultrastructure , Paranasal Sinus Neoplasms/chemistry , Paranasal Sinus Neoplasms/ultrastructure , Vimentin/analysis , Calponins
Common Bile Duct Neoplasms/complications , Jaundice, Obstructive/etiology , Neurofibroma/complications , Adult , Biliary Tract Surgical Procedures , Common Bile Duct Neoplasms/pathology , Common Bile Duct Neoplasms/surgery , Female , Humans , Jaundice, Obstructive/surgery , Neurofibroma/pathology , Neurofibroma/surgery
To observe the association between MITEs and genes in details, 82 kb of the rice genomic DNA were sequenced, and 10 genes were identified by similarity search. It was found that miniature inverted-repeat transposable elements (MITEs) were located upstream of the genes identified without exception, regardless of their directions of transcription. One MITE was found to be located between two 5' regions of the genes with opposite orientation of transcription, indicating that it was shared by them. The positional patterns of MITEs may therefore serve as an aid for preliminary screening of genes.
