RESUMEN
We aimed to assess the regulatory role of circular RNA (circRNA)-9119 (circ9119) in ovarian cancer (OC) cell viability. The expression of circ9119 was clearly reduced in OC tissues and cell lines, whereas the microRNA-21-5p (miR-21) levels were elevated compared with those in normal healthy control tissues and immortalized fallopian epithelial cell line FTE187. Further, circ9119 was overexpressed, causing a notable decrease in the viability and proliferation of OC cells and an increase in apoptosis. Further study showed that circ9119 upregulation resulted in a decrease in miR-21 levels. Bioinformatics forecasting (starBase and TargetScan) and dual luciferase reporter assay demonstrated that circ9119 acts as an miR-21 sponge. Recovery of miR-21 expression in circ9119-overexpressing OC cells showed that miR-21 exhibited the opposite effect on circ9119; moreover, its recovery could suppress the effects of circ9119 overexpression, recover cell proliferation, and reduce apoptosis. Furthermore, miR-21 was found to target phosphatase and tensin homologue (PTEN) 3' untranslated region. PTEN protein and mRNA expression was reduced in OC tissues and cells, whereas it was increased on transfection with an miR-21 inhibitor. Thus, circ9119 could regulate cell proliferation and apoptosis of OC cells via by acting as an miR-21 sponge and targeting the PTEN-Akt pathway.
Asunto(s)
MicroARNs/genética , Neoplasias Ováricas/genética , Neoplasias Ováricas/patología , ARN Circular/genética , Transducción de Señal/genética , Regiones no Traducidas 3' , Adulto , Animales , Apoptosis/genética , Línea Celular Tumoral , Proliferación Celular , Supervivencia Celular/genética , Femenino , Humanos , Ratones , Ratones Endogámicos BALB C , MicroARNs/antagonistas & inhibidores , Persona de Mediana Edad , Fosfohidrolasa PTEN/genética , Proteínas Proto-Oncogénicas c-akt/genética , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
LncRNA MIR4435-2HG is characterized as an oncogene in lung cancer. However, its role in ovarian carcinoma (OC) is unclear. In this study, we aimed to investigate the role of MIR4435-2HG in OC. We found that both MIR4435-2HG and transforming growth factor beta 1 (TGF-ß1) were upregulated in OC. MIR4435-2HG is associated with tumor metastasis but not with tumor size. Upregulation of MIR4435-2HG distinguished early stage (Stage I and II) OC patients from healthy controls. Correlation analysis showed that plasma levels of MIR4435-2HG and TGF-ß1 were positively correlated only in OC patients. qPCR and western blot analysis results showed that MIR4435-2HG overexpression led to upregulation of TGF-ß1 in OC cells, while TGF-ß1 treatment did not significantly affect MIR4435-2HG expression. Transwell invasion and migration assays showed that MIR4435-2HG and TGF-ß1 promoted the invasion and migration of OC cells while TGF-ß inhibitor suppressed the invasion and migration of these cells. Further analysis of the Transwell invasion and migration assay results showed that TGF-ß inhibitor reduced the effects of MIR4435-2HG overexpression. Therefore, our results suggested that lncRNA MIR4435-2HG may promote OC by upregulating TGF-ß1. Further characterization of the functions of MIR4435-2HG in OC may provide novel targets for cancer therapies.
Asunto(s)
Biomarcadores de Tumor/fisiología , Carcinoma/diagnóstico , MicroARNs/fisiología , Neoplasias Ováricas/diagnóstico , ARN Largo no Codificante/fisiología , Adulto , Anciano , Biomarcadores de Tumor/sangre , Carcinoma/sangre , Línea Celular Tumoral , Femenino , Humanos , MicroARNs/sangre , Persona de Mediana Edad , Neoplasias Ováricas/sangre , ARN Largo no Codificante/sangre , Factor de Crecimiento Transformador beta1/sangreRESUMEN
WiFi fingerprinting indoor positioning systems have extensive applied prospects. However, a vast amount of data in a particular environment has to be gathered to establish a fingerprinting database. Deficiencies of these systems are the lack of universality of multipath effects and a burden of heavy workload on fingerprint storage. Thus, this paper presents a novel Random Forest fingerprinting localization (RFFP) method using channel state information (CSI), which utilizes the Random Forest model trained in the offline stage as fingerprints in order to economize memory space and possess a good anti-multipath characteristic. Furthermore, a series of specific experiments are conducted in a microwave anechoic chamber and an office to detail the localization performance of RFFP with different wireless channel circumstances, system parameters, algorithms, and input datasets. In addition, compared with other algorithms including K-Nearest-Neighbor (KNN), Weighted K-Nearest-Neighbor (WKNN), REPTree, CART, and J48, the RFFP method provides far greater classification accuracy as well as lower mean location error. The proposed method offers outstanding comprehensive performance including accuracy, robustness, low workload, and better anti-multipath-fading.